JP6297134B2 - プトレシン生産性を有する微生物及びそれを用いたプトレシン生産方法 - Google Patents
プトレシン生産性を有する微生物及びそれを用いたプトレシン生産方法 Download PDFInfo
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- JP6297134B2 JP6297134B2 JP2016504226A JP2016504226A JP6297134B2 JP 6297134 B2 JP6297134 B2 JP 6297134B2 JP 2016504226 A JP2016504226 A JP 2016504226A JP 2016504226 A JP2016504226 A JP 2016504226A JP 6297134 B2 JP6297134 B2 JP 6297134B2
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Description
[1]配列番号21又は23で表されるアミノ酸配列を含むタンパク質の活性が強化されるように改変された、プトレシン生産能を有する微生物。
[2]上記微生物がさらに、オルニチンカルバモイルトランスフェラーゼ(ArgF)及びグルタメート排出に関与するタンパク質(NCgl1221)の活性が内在的活性より低下するように改変され、オルニチンデカルボキシラーゼ(ODC)の活性が強化された、上記[1]に記載のプトレシン生産能を有する微生物。
[3]上記オルニチンカルバモイルトランスフェラーゼ(ArgF)が配列番号29で表されるアミノ酸配列を含み、グルタメート排出に関与するタンパク質(NCgl1221)が配列番号30で表されるアミノ酸配列を含み、オルニチンデカルボキシラーゼ(ODC)が配列番号33で表されるアミノ酸配列を含む、上記[2]に記載のプトレシン生産能を有する微生物。
[4]上記微生物がさらに、アセチルガンマグルタミルホスフェートレダクターゼ(ArgC)、アセチルグルタメートシンターゼ又はオルニチンアセチルトランスフェラーゼ(ArgJ)、アセチルグルタメートキナーゼ(ArgB)、及びアセチルオルニチンアミノトランスフェラーゼ(ArgD)の活性が内在的活性より強化されるように改変された、上記[1]に記載のプトレシン生産能を有する微生物。
[5]上記アセチルガンマグルタミルホスフェートレダクターゼ(ArgC)、アセチルグルタメートシンターゼ又はオルニチンアセチルトランスフェラーゼ(ArgJ)、アセチルグルタメートキナーゼ(ArgB)、及びアセチルオルニチンアミノトランスフェラーゼ(ArgD)がそれぞれ配列番号25、26、27及び28で表されるアミノ酸配列を含む、上記[4]に記載のプトレシン生産能を有する微生物。
[6]上記微生物がさらに、アセチルトランスフェラーゼ(NCgl1469)の活性が低下したものである、上記[1]に記載のプトレシン生産能を有する微生物。
[7]上記アセチルトランスフェラーゼ(NCgl1469)が配列番号31又は32で表されるアミノ酸配列を含む、上記[6]に記載のプトレシン生産能を有する微生物。
[8]上記微生物がエシェリキア属微生物又はコリネ型微生物である、上記[1]に記載のプトレシン生産能を有する微生物。
[9]上記微生物が大腸菌又はコリネバクテリウムグルタミカムである、上記[8]に記載のプトレシン生産能を有する微生物。
[10](i)上記[1]〜[9]のいずれかによるプトレシン生産能を有する微生物を培養して培養物を得るステップと、
(ii)上記培養された微生物又は培養物からプトレシンを回収するステップとを含む、プトレシンの生産方法。
上記目的を達成するために、本発明の一態様は、配列番号21又は23で表されるアミノ酸配列を含むタンパク質の活性が強化されるように改変された、プトレシン生産能を有する微生物を提供する。
プトレシン生成能を有するコリネバクテリウム属微生物を作製するために、特許文献6に記載のとおり、オルニチンからアルギニンへの生合成経路を遮断し、グルタメートからオルニチンを生産する生合成経路の活性を強化し、オルニチンデカルボキシラーゼ(ODC)を外来から導入することにより、プトレシン生産能が付与された微生物を作製した。
プトレシン生成能を有する他のコリネバクテリウム属微生物として、参照例1で作製されたコリネバクテリウムグルタミカムKCCM11138Pにおいて、アセチルトランスフェラーゼ(acetyltransferase)であるNCgl1469をコードする遺伝子を欠失させてN−アセチルプトレシンを生成させないことにより、プトレシン生産量が増加したコリネバクテリウムグルタミカム菌株を作製した。
コリネバクテリウムグルタミカムにはプトレシン生合成経路がないが、外部からオルニチンデカルボキシラーゼを導入してプトレシン合成能を有するようになると、プトレシンが生成されて細胞外にプトレシンが排出される。これは、コリネバクテリウム属微生物の数多くの膜タンパク質の中にプトレシン排出通路として作用するトランスポータータンパク質が存在することを示すものである。
<2−1>ATCC13032ベースのプトレシン生産菌株からNCgl2522欠損菌株の作製
コリネバクテリウムグルタミカムATCC13032由来のNCgl2522がプトレシン排出に関与しているか否かを確認するために、NCgl2522をコードする遺伝子を欠損させるベクターを作製した。
コリネバクテリウムグルタミカムATCC13032ベースのプトレシン生産菌株であるKCCM11138P及びKCCM11240Pと同じ遺伝子型(genotype)を有するコリネバクテリウムグルタミカムATCC13869ベースのプトレシン生産菌株DAB12−a(ArgF欠損,NCgl1221欠損,大腸菌speC導入,argオペロンプロモーター置換,参照例1参照)及びDAB12−b(ArgF欠損,NCgl1221欠損,大腸菌speC導入,argオペロンプロモーター置換,NCgl1469欠損,参照例2参照)を対象にNCgl2522欠損菌株を作製した。
プトレシン生産菌株においてNCgl2522欠損がプトレシン生産に及ぼす効果を確認するために、実施例<2−1>及び<2−2>で作製されたコリネバクテリウムグルタミカム変異株を対象にプトレシン生産能を比較した。
<3−1>ATCC13032の染色体におけるトランスポゾン遺伝子内へのNCgl2522の導入
プトレシン生成能を有するコリネバクテリウム属微生物KCCM11138PにおいてNCgl2522遺伝子(自己プロモーター部位を含む)の染色体内への追加挿入によるプトレシン高生産効果を確認するために、NCgl2522をトランスポゾン遺伝子内に導入した。コリネバクテリウム属微生物のトランスポゾン遺伝子部位を用いて染色体内への遺伝子導入を可能にする形質転換用ベクターpDZTn(特許文献9)を用いた。
プトレシン生産菌株においてNCgl2522活性を強化するために、染色体内のNCgl2522の開始コドンの前にCJ7プロモーター(特許文献5)を導入した。
コリネバクテリウムグルタミカムATCC13869由来のプトレシン菌株からNCgl2522遺伝子の染色体内への追加挿入によるプトレシン高生産効果を確認するために、NCgl2522(プロモーター部位を含む)をトランスポゾン遺伝子内に導入することにした。NCgl2522遺伝子は、ATCC13869菌株の染色体を鋳型とし、配列番号17及び10のプライマー対を用いて約1.97kbの遺伝子断片を増幅した(表6参照)。ここで、PCR反応は、94℃で30秒間の変性、55℃で30秒間のアニーリング、及び72℃で30秒間又は2分間の伸長の過程を30回繰り返した。こうして得られたNCgl2522のPCR断片をXhoIで処理したpDZTnベクターにフュージョンクローニングした。フュージョンクローニングには、In−Fusion HD Cloning Kit(Clontech)を用いた。結果として得られたプラスミドをpDZTn−2’NCgl2522と命名した。
コリネバクテリウムグルタミカムATCC13869由来のNCgl2522の開始コドンの前にCJ7プロモーターを導入するために、実施例<3−2>と同様に、コリネバクテリウムグルタミカムATCC13869のゲノムDNAを鋳型とし、下記表7に示す3対の各プライマーを用いてPCRを行うことにより、CJ7プロモーター部位、そのN末端部位及びC末端部位のPCR断片をそれぞれ増幅し、その後それを電気泳動することにより目的とする断片を得た。ここで、PCR反応は、94℃で30秒間の変性、55℃で30秒間のアニーリング、及び72℃で30秒間の伸長の過程を30回繰り返した。こうして得られたCJ7プロモーター部位、そのN末端部位及びC末端部位のPCR断片をBamHIとXbaIで処理したpDZベクターにフュージョンクローニングした。フュージョンクローニングには、In−Fusion HD Cloning Kit(Clontech)を用いた。結果として得られたプラスミドをpDZ−P(CJ7)−2’NCgl2522と命名した。
プトレシン生産菌株においてプロモーター置換によるNCgl2522活性の強化がプトレシン生産に及ぼす効果を確認するために、実施例<3−1>〜<3−4>で作製した6種のコリネバクテリウムグルタミカム変異株(KCCM11138P Tn:1’NCgl2522、KCCM11138P P(CJ7)−NCgl2522、KCCM11240P P(CJ7)−NCgl2522、DAB12−a Tn:2’NCgl2522、DAB12−a P(CJ7)−NCgl2522、及びDAB12−b P(CJ7)−NCgl2522)と4種の母菌株(KCCM11138P、KCCM11240P、DAB12−a、及びDAB12−b)のプトレシン生産能を比較した。各菌株を実施例2−3と同様に培養し、その後各培養物から生産されたプトレシンの濃度を測定した。その結果を下記表8に示す。
NCgl2522活性が強化されたコリネバクテリウムグルタミカム変異株においてプトレシン排出能が向上することにより細胞内のプトレシン濃度が低下したか否かを確認するために、コリネバクテリウムグルタミカム変異株KCCM11138P Tn:1’NCgl2522及び母菌株KCCM11138Pを対象に有機溶媒を用いた抽出方法で細胞内のプトレシン濃度を測定した。細胞内の代謝物質(intracellular metabolite)の分析は、非特許文献11に記載の方法により行った。
NCgl2522のプトレシン耐性に対する影響を確認するために、前述したように作製したKCCM11240P、KCCM11240P△NCgl2522及びKCCM11240P P(CJ7)−NCgl2522菌株を対象にプトレシン耐性度を評価した。
プトレシン生合成経路を有する大腸菌野生型菌株W3110においてコリネバクテリウムグルタミカムATCC13032のNCgl2522発現時のプトレシン生産増加効果を確認するために、W3110にプトレシン合成酵素であるspeC発現ベクターとNCgl2522発現ベクターをそれぞれ導入した。
Claims (10)
- 改変されていない微生物のタンパク質の活性と比較して、配列番号21又は23で表されるアミノ酸配列を含むタンパク質の活性が強化されるように改変された、プトレシン生産能を有する微生物。
- 前記微生物がさらに、オルニチンカルバモイルトランスフェラーゼ(ArgF)及びグルタメート排出に関与するタンパク質(NCgl1221)の活性が改変されていない微生物のタンパク質の活性より低下するように改変され、オルニチンデカルボキシラーゼ(ODC)の活性が改変されていない微生物のタンパク質の活性と比較して強化された、請求項1に記載のプトレシン生産能を有する微生物。
- 前記オルニチンカルバモイルトランスフェラーゼ(ArgF)が配列番号29で表されるアミノ酸配列を含み、グルタメート排出に関与するタンパク質(NCgl1221)が配列番号30で表されるアミノ酸配列を含み、オルニチンデカルボキシラーゼ(ODC)が配列番号33で表されるアミノ酸配列を含む、請求項2に記載のプトレシン生産能を有する微生物。
- 前記微生物がさらに、アセチルガンマグルタミルホスフェートレダクターゼ(ArgC)、アセチルグルタメートシンターゼ又はオルニチンアセチルトランスフェラーゼ(ArgJ)、アセチルグルタメートキナーゼ(ArgB)、及びアセチルオルニチンアミノトランスフェラーゼ(ArgD)の活性が内在的活性より強化されるように改変された、請求項1に記載のプトレシン生産能を有する微生物。
- 前記アセチルガンマグルタミルホスフェートレダクターゼ(ArgC)、アセチルグルタメートシンターゼ又はオルニチンアセチルトランスフェラーゼ(ArgJ)、アセチルグルタメートキナーゼ(ArgB)、及びアセチルオルニチンアミノトランスフェラーゼ(ArgD)がそれぞれ配列番号25、26、27及び28で表されるアミノ酸配列を含む、請求項4に記載のプトレシン生産能を有する微生物。
- 前記微生物がさらに、アセチルトランスフェラーゼ(NCgl1469)の活性が改変されていない微生物のタンパク質の活性と比較して低下したものである、請求項1に記載のプトレシン生産能を有する微生物。
- 前記アセチルトランスフェラーゼ(NCgl1469)が配列番号31又は32で表されるアミノ酸配列を含む、請求項6に記載のプトレシン生産能を有する微生物。
- 前記微生物がエシェリキア属微生物又はコリネ型微生物である、請求項1に記載のプトレシン生産能を有する微生物。
- 前記微生物が大腸菌又はコリネバクテリウムグルタミカムである、請求項8に記載のプトレシン生産能を有する微生物。
- (i)請求項1〜9のいずれかに記載のプトレシン生産能を有する微生物を培養して培養物を得るステップと、
(ii)前記培養された微生物又は培養物からプトレシンを回収するステップとを含む、プトレシンの生産方法。
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JP2019162126A (ja) * | 2014-04-25 | 2019-09-26 | シージェイ チェイルジェダン コーポレーション | ジアミンを生産する微生物及びそれらを用いてジアミンを生産する方法 |
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US20170002386A1 (en) | 2017-01-05 |
CN105492593A (zh) | 2016-04-13 |
BR112015018599B1 (pt) | 2022-12-13 |
US11053524B2 (en) | 2021-07-06 |
TWI657142B (zh) | 2019-04-21 |
CN105492593B (zh) | 2019-08-09 |
MY172080A (en) | 2019-11-13 |
EP2977443A1 (en) | 2016-01-27 |
JP2016514466A (ja) | 2016-05-23 |
US10221433B2 (en) | 2019-03-05 |
AU2014238707A1 (en) | 2015-07-23 |
US20190136274A1 (en) | 2019-05-09 |
AU2014238707B2 (en) | 2017-08-31 |
TWI632238B (zh) | 2018-08-11 |
HUE048852T2 (hu) | 2020-08-28 |
KR101607741B1 (ko) | 2016-03-31 |
RU2015142261A (ru) | 2017-04-26 |
EP2977443A4 (en) | 2016-09-07 |
RU2665825C2 (ru) | 2018-09-04 |
BR112015018599A2 (pt) | 2017-08-22 |
TW201441367A (zh) | 2014-11-01 |
KR20140115244A (ko) | 2014-09-30 |
EP2977443B1 (en) | 2020-04-08 |
WO2014148743A1 (ko) | 2014-09-25 |
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