CA2898317C - Acyl-acp reductase with improved properties - Google Patents

Acyl-acp reductase with improved properties Download PDF

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Publication number
CA2898317C
CA2898317C CA2898317A CA2898317A CA2898317C CA 2898317 C CA2898317 C CA 2898317C CA 2898317 A CA2898317 A CA 2898317A CA 2898317 A CA2898317 A CA 2898317A CA 2898317 C CA2898317 C CA 2898317C
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CA
Canada
Prior art keywords
mutation
fatty
fatty alcohol
seq
polypeptide
Prior art date
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Active
Application number
CA2898317A
Other languages
English (en)
French (fr)
Other versions
CA2898317A1 (en
Inventor
Mathew Rude
Na Trinh
Andreas Schirmer
Jacob GANO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Priority to CA3143642A priority Critical patent/CA3143642A1/en
Publication of CA2898317A1 publication Critical patent/CA2898317A1/en
Application granted granted Critical
Publication of CA2898317C publication Critical patent/CA2898317C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
CA2898317A 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties Active CA2898317C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3143642A CA3143642A1 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
PCT/US2014/011859 WO2014113571A2 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3143642A Division CA3143642A1 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Publications (2)

Publication Number Publication Date
CA2898317A1 CA2898317A1 (en) 2014-07-24
CA2898317C true CA2898317C (en) 2022-03-01

Family

ID=50473752

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2898317A Active CA2898317C (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties
CA3143642A Pending CA3143642A1 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3143642A Pending CA3143642A1 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Country Status (13)

Country Link
US (6) US9683219B2 (enExample)
EP (3) EP2946009B1 (enExample)
JP (5) JP6063585B2 (enExample)
KR (4) KR20210072147A (enExample)
CN (3) CN109897834A (enExample)
AU (3) AU2014207522B2 (enExample)
BR (1) BR112015017005A2 (enExample)
CA (2) CA2898317C (enExample)
CO (1) CO7461134A2 (enExample)
ES (2) ES2600354T3 (enExample)
MX (2) MX356997B (enExample)
MY (1) MY167434A (enExample)
WO (1) WO2014113571A2 (enExample)

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CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
CN109182238A (zh) 2012-08-10 2019-01-11 嘉吉有限公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
WO2014113571A2 (en) * 2013-01-16 2014-07-24 Ls9, Inc. Acyl-acp reductase with improved properties
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
BR112017001105A2 (pt) * 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
EP3844269A4 (en) * 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONOUNSATURATED FATTY ACIDS IN BACTERIA
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
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ES2528482T3 (es) 2006-05-19 2015-02-10 Ls9, Inc. Producción de ácidos grasos y derivados de los mismos
WO2008119082A2 (en) 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
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Also Published As

Publication number Publication date
JP2017042170A (ja) 2017-03-02
US20200010813A1 (en) 2020-01-09
AU2019202790A1 (en) 2019-05-16
KR20210072147A (ko) 2021-06-16
CA2898317A1 (en) 2014-07-24
JP2021006067A (ja) 2021-01-21
AU2014207522B2 (en) 2016-12-15
US20240117324A1 (en) 2024-04-11
US20170342388A1 (en) 2017-11-30
EP3103867B1 (en) 2018-05-16
ES2675224T3 (es) 2018-07-09
CN105051189A (zh) 2015-11-11
JP7094343B2 (ja) 2022-07-01
US20220090028A1 (en) 2022-03-24
KR20170066694A (ko) 2017-06-14
BR112015017005A2 (pt) 2017-08-15
CN109897834A (zh) 2019-06-18
KR20150105471A (ko) 2015-09-16
KR101746040B1 (ko) 2017-06-13
US9683219B2 (en) 2017-06-20
WO2014113571A2 (en) 2014-07-24
JP2019141083A (ja) 2019-08-29
MY167434A (en) 2018-08-28
MX2015009138A (es) 2015-11-06
CN110387352A (zh) 2019-10-29
JP2016503663A (ja) 2016-02-08
WO2014113571A3 (en) 2014-11-13
AU2014207522A1 (en) 2015-05-07
US20160348080A1 (en) 2016-12-01
EP3385375A1 (en) 2018-10-10
EP2946009A2 (en) 2015-11-25
US20150361454A1 (en) 2015-12-17
KR102082247B1 (ko) 2020-02-28
AU2017200596A1 (en) 2017-02-23
MX356997B (es) 2018-06-22
EP3385375B1 (en) 2021-06-16
US11130944B2 (en) 2021-09-28
KR102265148B1 (ko) 2021-06-15
CA3143642A1 (en) 2014-07-24
JP6523393B2 (ja) 2019-05-29
US12173329B2 (en) 2024-12-24
CN105051189B (zh) 2019-08-06
US10208294B2 (en) 2019-02-19
AU2017200596B2 (en) 2019-01-24
JP2017209118A (ja) 2017-11-30
KR20200020998A (ko) 2020-02-26
US9873865B2 (en) 2018-01-23
ES2600354T3 (es) 2017-02-08
EP3103867A1 (en) 2016-12-14
JP6063585B2 (ja) 2017-01-18
JP6926143B2 (ja) 2021-08-25
CO7461134A2 (es) 2015-11-30
MX346830B (es) 2017-04-03
EP2946009B1 (en) 2016-07-27
JP6211162B2 (ja) 2017-10-11

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