CA2886460C - Leader peptides for expressing a protein of interest in an eukaryotic host cell - Google Patents
Leader peptides for expressing a protein of interest in an eukaryotic host cell Download PDFInfo
- Publication number
- CA2886460C CA2886460C CA2886460A CA2886460A CA2886460C CA 2886460 C CA2886460 C CA 2886460C CA 2886460 A CA2886460 A CA 2886460A CA 2886460 A CA2886460 A CA 2886460A CA 2886460 C CA2886460 C CA 2886460C
- Authority
- CA
- Canada
- Prior art keywords
- poi
- leader
- seq
- amino acid
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3212565A CA3212565A1 (en) | 2012-10-29 | 2013-10-29 | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12190361.1 | 2012-10-29 | ||
| EP12190361 | 2012-10-29 | ||
| PCT/EP2013/072572 WO2014067926A1 (en) | 2012-10-29 | 2013-10-29 | Expression sequences |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3212565A Division CA3212565A1 (en) | 2012-10-29 | 2013-10-29 | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2886460A1 CA2886460A1 (en) | 2014-05-08 |
| CA2886460C true CA2886460C (en) | 2023-11-14 |
Family
ID=47115533
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2886460A Active CA2886460C (en) | 2012-10-29 | 2013-10-29 | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
| CA3212565A Pending CA3212565A1 (en) | 2012-10-29 | 2013-10-29 | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3212565A Pending CA3212565A1 (en) | 2012-10-29 | 2013-10-29 | Leader peptides for expressing a protein of interest in an eukaryotic host cell |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US10160988B2 (enExample) |
| EP (2) | EP3130598B1 (enExample) |
| JP (2) | JP6556625B2 (enExample) |
| KR (1) | KR102079293B1 (enExample) |
| CN (1) | CN104837857B (enExample) |
| AU (1) | AU2013341049B2 (enExample) |
| BR (1) | BR112015008289A2 (enExample) |
| CA (2) | CA2886460C (enExample) |
| DK (1) | DK2912053T3 (enExample) |
| ES (2) | ES2610990T3 (enExample) |
| HU (1) | HUE033282T2 (enExample) |
| IL (2) | IL238420B (enExample) |
| IN (1) | IN2015DN02771A (enExample) |
| PL (1) | PL2912053T3 (enExample) |
| SG (1) | SG11201502745XA (enExample) |
| WO (1) | WO2014067926A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3130598B1 (en) * | 2012-10-29 | 2020-02-12 | Lonza Ltd | Expression sequences |
| CN104312933B (zh) * | 2014-10-17 | 2017-03-29 | 江南大学 | 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法 |
| CA2992170C (en) * | 2015-08-05 | 2022-08-16 | Lonza Ltd | Artificial pg1-x promoters |
| IL261411B2 (en) | 2016-03-02 | 2024-03-01 | Lonza Ag | Improved fermentation process |
| KR20250121449A (ko) * | 2017-03-10 | 2025-08-12 | 볼트 쓰레즈, 인크. | 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 |
| EP3630940A1 (en) | 2017-05-31 | 2020-04-08 | Universität für Bodenkultur Wien | Yeast expressing a synthetic calvin cycle |
| BR112020016258A2 (pt) | 2018-02-12 | 2020-12-15 | Lonza Ltd | Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira |
| WO2020106600A1 (en) | 2018-11-19 | 2020-05-28 | Basf Se | Leader sequence for yeast |
| ES2921137T5 (en) | 2019-01-11 | 2025-05-06 | Lonza Ag | Carbon-source regulated protein production in a recombinant host cell |
| WO2020200415A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Mut- methylotrophic yeast |
| US12270034B2 (en) | 2019-04-01 | 2025-04-08 | Universität Für Bodenkultur Wien | Mut- methylotrophic yeast |
| WO2020200414A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Protein production in mut-methylotrophic yeast |
| CN110760003A (zh) * | 2019-09-10 | 2020-02-07 | 广东药科大学 | 一种抗her3单链抗体的制备方法 |
| ES3034258T3 (en) | 2020-04-01 | 2025-08-14 | Lonza Ag | Helper factors for expressing proteins in yeast |
| KR20230075436A (ko) | 2020-09-30 | 2023-05-31 | 론자 리미티드 | 번역 인자를 과발현하는 숙주 세포 |
| AU2021411733A1 (en) | 2020-12-29 | 2023-07-06 | Bisy Gmbh | Novel yeast strains |
| WO2023007468A1 (en) * | 2021-07-30 | 2023-02-02 | Helaina, Inc. | Methods and compositions for protein synthesis and secretion |
| EP4638756A1 (en) | 2022-12-20 | 2025-10-29 | Lonza Ltd. | Host cells with pat1 knockout for increased specific protein productivity |
| EP4642916A2 (en) * | 2022-12-30 | 2025-11-05 | Biotalys NV | Secretion signals |
| WO2026009000A1 (en) | 2024-07-04 | 2026-01-08 | Solasta Bio Limited | Methods for production of insecticidal neuropeptides |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1984001153A1 (en) | 1982-09-15 | 1984-03-29 | Collaborative Res Inc | Production of interferon in yeast by use of invertase promoter |
| US5010182A (en) | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
| CA1340772C (en) | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
| DE69133066T2 (de) | 1990-01-16 | 2003-03-06 | Centro De Ingenieria Genetica Y Biotecnologia, Cubanacan Playa | Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen |
| US5268273A (en) | 1990-12-14 | 1993-12-07 | Phillips Petroleum Company | Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same |
| DK82893D0 (da) | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
| JP2010524440A (ja) | 2007-04-20 | 2010-07-22 | ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング | 発現系 |
| US7741075B2 (en) | 2008-06-06 | 2010-06-22 | Board Of Regents Of The University Of Nebraska | Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display |
| US8440456B2 (en) | 2009-05-22 | 2013-05-14 | Vib, Vzw | Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins |
| EP2258855A1 (en) * | 2009-05-28 | 2010-12-08 | Universität für Bodenkultur Wien | Expression sequences |
| DE102009055015B4 (de) | 2009-12-18 | 2013-12-24 | Technische Universität Dresden | Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei |
| EP3590950A1 (en) * | 2011-05-09 | 2020-01-08 | Ablynx NV | Method for the production of immunoglobulin single varible domains |
| EP3130598B1 (en) * | 2012-10-29 | 2020-02-12 | Lonza Ltd | Expression sequences |
-
2013
- 2013-10-29 EP EP16184950.0A patent/EP3130598B1/en active Active
- 2013-10-29 US US14/435,581 patent/US10160988B2/en active Active
- 2013-10-29 KR KR1020157009776A patent/KR102079293B1/ko active Active
- 2013-10-29 CN CN201380056279.9A patent/CN104837857B/zh active Active
- 2013-10-29 WO PCT/EP2013/072572 patent/WO2014067926A1/en not_active Ceased
- 2013-10-29 BR BR112015008289A patent/BR112015008289A2/pt not_active Application Discontinuation
- 2013-10-29 ES ES13785840.3T patent/ES2610990T3/es active Active
- 2013-10-29 SG SG11201502745XA patent/SG11201502745XA/en unknown
- 2013-10-29 AU AU2013341049A patent/AU2013341049B2/en not_active Ceased
- 2013-10-29 PL PL13785840T patent/PL2912053T3/pl unknown
- 2013-10-29 EP EP13785840.3A patent/EP2912053B1/en active Active
- 2013-10-29 CA CA2886460A patent/CA2886460C/en active Active
- 2013-10-29 DK DK13785840.3T patent/DK2912053T3/da active
- 2013-10-29 CA CA3212565A patent/CA3212565A1/en active Pending
- 2013-10-29 HU HUE13785840A patent/HUE033282T2/en unknown
- 2013-10-29 JP JP2015540103A patent/JP6556625B2/ja active Active
- 2013-10-29 IN IN2771DEN2015 patent/IN2015DN02771A/en unknown
- 2013-10-29 ES ES16184950T patent/ES2789299T3/es active Active
-
2015
- 2015-04-21 IL IL238420A patent/IL238420B/en active IP Right Grant
-
2018
- 2018-01-02 US US15/860,186 patent/US11359223B2/en active Active
-
2019
- 2019-04-01 JP JP2019069948A patent/JP2019141063A/ja not_active Withdrawn
- 2019-05-16 IL IL266700A patent/IL266700B/en unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20180802 |
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| MPN | Maintenance fee for patent paid |
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| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 12TH ANNIV.) - STANDARD Year of fee payment: 12 |
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| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20251023 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251023 |