JP6556625B2 - 発現配列 - Google Patents

発現配列 Download PDF

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Publication number
JP6556625B2
JP6556625B2 JP2015540103A JP2015540103A JP6556625B2 JP 6556625 B2 JP6556625 B2 JP 6556625B2 JP 2015540103 A JP2015540103 A JP 2015540103A JP 2015540103 A JP2015540103 A JP 2015540103A JP 6556625 B2 JP6556625 B2 JP 6556625B2
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JP
Japan
Prior art keywords
seq
protein
poi
amino acid
nucleic acid
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JP2015540103A
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English (en)
Japanese (ja)
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JP2015533286A (ja
Inventor
ガッセル、ブリジット
マッタノビヒ、ディサールド
ハイス、シルビア
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Lonza AG
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Lonza AG
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2015540103A 2012-10-29 2013-10-29 発現配列 Active JP6556625B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12190361.1 2012-10-29
EP12190361 2012-10-29
PCT/EP2013/072572 WO2014067926A1 (en) 2012-10-29 2013-10-29 Expression sequences

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019069948A Division JP2019141063A (ja) 2012-10-29 2019-04-01 発現配列

Publications (2)

Publication Number Publication Date
JP2015533286A JP2015533286A (ja) 2015-11-24
JP6556625B2 true JP6556625B2 (ja) 2019-08-07

Family

ID=47115533

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2015540103A Active JP6556625B2 (ja) 2012-10-29 2013-10-29 発現配列
JP2019069948A Withdrawn JP2019141063A (ja) 2012-10-29 2019-04-01 発現配列

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019069948A Withdrawn JP2019141063A (ja) 2012-10-29 2019-04-01 発現配列

Country Status (16)

Country Link
US (2) US10160988B2 (enExample)
EP (2) EP3130598B1 (enExample)
JP (2) JP6556625B2 (enExample)
KR (1) KR102079293B1 (enExample)
CN (1) CN104837857B (enExample)
AU (1) AU2013341049B2 (enExample)
BR (1) BR112015008289A2 (enExample)
CA (2) CA2886460C (enExample)
DK (1) DK2912053T3 (enExample)
ES (2) ES2610990T3 (enExample)
HU (1) HUE033282T2 (enExample)
IL (2) IL238420B (enExample)
IN (1) IN2015DN02771A (enExample)
PL (1) PL2912053T3 (enExample)
SG (1) SG11201502745XA (enExample)
WO (1) WO2014067926A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3130598B1 (en) * 2012-10-29 2020-02-12 Lonza Ltd Expression sequences
CN104312933B (zh) * 2014-10-17 2017-03-29 江南大学 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法
CA2992170C (en) * 2015-08-05 2022-08-16 Lonza Ltd Artificial pg1-x promoters
IL261411B2 (en) 2016-03-02 2024-03-01 Lonza Ag Improved fermentation process
KR20250121449A (ko) * 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
EP3630940A1 (en) 2017-05-31 2020-04-08 Universität für Bodenkultur Wien Yeast expressing a synthetic calvin cycle
BR112020016258A2 (pt) 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
WO2020106600A1 (en) 2018-11-19 2020-05-28 Basf Se Leader sequence for yeast
ES2921137T5 (en) 2019-01-11 2025-05-06 Lonza Ag Carbon-source regulated protein production in a recombinant host cell
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
US12270034B2 (en) 2019-04-01 2025-04-08 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
CN110760003A (zh) * 2019-09-10 2020-02-07 广东药科大学 一种抗her3单链抗体的制备方法
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
KR20230075436A (ko) 2020-09-30 2023-05-31 론자 리미티드 번역 인자를 과발현하는 숙주 세포
AU2021411733A1 (en) 2020-12-29 2023-07-06 Bisy Gmbh Novel yeast strains
WO2023007468A1 (en) * 2021-07-30 2023-02-02 Helaina, Inc. Methods and compositions for protein synthesis and secretion
EP4638756A1 (en) 2022-12-20 2025-10-29 Lonza Ltd. Host cells with pat1 knockout for increased specific protein productivity
EP4642916A2 (en) * 2022-12-30 2025-11-05 Biotalys NV Secretion signals
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984001153A1 (en) 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DE69133066T2 (de) 1990-01-16 2003-03-06 Centro De Ingenieria Genetica Y Biotecnologia, Cubanacan Playa Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
JP2010524440A (ja) 2007-04-20 2010-07-22 ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング 発現系
US7741075B2 (en) 2008-06-06 2010-06-22 Board Of Regents Of The University Of Nebraska Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display
US8440456B2 (en) 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
EP2258855A1 (en) * 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DE102009055015B4 (de) 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
EP3590950A1 (en) * 2011-05-09 2020-01-08 Ablynx NV Method for the production of immunoglobulin single varible domains
EP3130598B1 (en) * 2012-10-29 2020-02-12 Lonza Ltd Expression sequences

Also Published As

Publication number Publication date
JP2019141063A (ja) 2019-08-29
AU2013341049B2 (en) 2017-07-20
US20180135092A1 (en) 2018-05-17
CA3212565A1 (en) 2014-05-08
EP3130598A2 (en) 2017-02-15
IL238420A0 (en) 2015-06-30
HUE033282T2 (en) 2017-11-28
US11359223B2 (en) 2022-06-14
DK2912053T3 (en) 2017-01-30
US10160988B2 (en) 2018-12-25
CN104837857B (zh) 2018-11-06
KR102079293B1 (ko) 2020-02-19
EP2912053A1 (en) 2015-09-02
CA2886460C (en) 2023-11-14
BR112015008289A2 (pt) 2017-11-28
EP2912053B1 (en) 2016-11-09
KR20150076160A (ko) 2015-07-06
IL238420B (en) 2019-05-30
PL2912053T3 (pl) 2017-05-31
US20150232904A1 (en) 2015-08-20
ES2610990T3 (es) 2017-05-04
JP2015533286A (ja) 2015-11-24
WO2014067926A1 (en) 2014-05-08
CA2886460A1 (en) 2014-05-08
EP3130598B1 (en) 2020-02-12
HK1208037A1 (en) 2016-02-19
EP3130598A3 (en) 2017-05-03
IL266700B (en) 2021-08-31
SG11201502745XA (en) 2015-05-28
IL266700A (en) 2019-07-31
IN2015DN02771A (enExample) 2015-09-04
CN104837857A (zh) 2015-08-12
AU2013341049A1 (en) 2015-04-02
ES2789299T3 (es) 2020-10-26

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