KR102079293B1 - 발현 서열 - Google Patents

발현 서열 Download PDF

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Publication number
KR102079293B1
KR102079293B1 KR1020157009776A KR20157009776A KR102079293B1 KR 102079293 B1 KR102079293 B1 KR 102079293B1 KR 1020157009776 A KR1020157009776 A KR 1020157009776A KR 20157009776 A KR20157009776 A KR 20157009776A KR 102079293 B1 KR102079293 B1 KR 102079293B1
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seq
amino acid
poi
leader
sequence
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Korean (ko)
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KR20150076160A (ko
Inventor
브리짓 개서
디트하드 마타노비치
실비아 하이스
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론자 리미티드
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76Albumins
    • C07K14/765Serum albumin, e.g. HSA
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020157009776A 2012-10-29 2013-10-29 발현 서열 Active KR102079293B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12190361.1 2012-10-29
EP12190361 2012-10-29
PCT/EP2013/072572 WO2014067926A1 (en) 2012-10-29 2013-10-29 Expression sequences

Publications (2)

Publication Number Publication Date
KR20150076160A KR20150076160A (ko) 2015-07-06
KR102079293B1 true KR102079293B1 (ko) 2020-02-19

Family

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Family Applications (1)

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KR1020157009776A Active KR102079293B1 (ko) 2012-10-29 2013-10-29 발현 서열

Country Status (16)

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US (2) US10160988B2 (enExample)
EP (2) EP2912053B1 (enExample)
JP (2) JP6556625B2 (enExample)
KR (1) KR102079293B1 (enExample)
CN (1) CN104837857B (enExample)
AU (1) AU2013341049B2 (enExample)
BR (1) BR112015008289A2 (enExample)
CA (2) CA3212565A1 (enExample)
DK (1) DK2912053T3 (enExample)
ES (2) ES2610990T3 (enExample)
HU (1) HUE033282T2 (enExample)
IL (2) IL238420B (enExample)
IN (1) IN2015DN02771A (enExample)
PL (1) PL2912053T3 (enExample)
SG (1) SG11201502745XA (enExample)
WO (1) WO2014067926A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013341049B2 (en) * 2012-10-29 2017-07-20 Lonza Ltd Expression sequences
CN104312933B (zh) * 2014-10-17 2017-03-29 江南大学 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法
WO2017021541A1 (en) * 2015-08-05 2017-02-09 Lonza Ltd Promoter variants
WO2017149065A1 (en) 2016-03-02 2017-09-08 Lonza Ltd Improved fermentation process
KR20250121449A (ko) * 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
EP3630940A1 (en) 2017-05-31 2020-04-08 Universität für Bodenkultur Wien Yeast expressing a synthetic calvin cycle
WO2019154686A1 (en) 2018-02-12 2019-08-15 Lonza Ltd Host cell for producing a protein of interest
CN113015782A (zh) * 2018-11-19 2021-06-22 巴斯夫欧洲公司 用于酵母的前导序列
DK3874047T4 (da) 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
EP3947695B1 (en) 2019-04-01 2023-09-06 Universität für Bodenkultur Wien Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
CN110760003A (zh) * 2019-09-10 2020-02-07 广东药科大学 一种抗her3单链抗体的制备方法
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
EP4222266A1 (en) 2020-09-30 2023-08-09 Lonza Ltd. Host cells overexpressing translational factors
AU2021411733A1 (en) 2020-12-29 2023-07-06 Bisy Gmbh Novel yeast strains
AU2022318574B2 (en) * 2021-07-30 2024-03-21 Helaina, Inc. Methods and compositions for protein synthesis and secretion
CN117794941A (zh) * 2021-07-30 2024-03-29 赫莱纳公司 用于蛋白质合成和分泌的方法和组合物
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
WO2024141641A2 (en) * 2022-12-30 2024-07-04 Biotalys NV Secretion signals

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0118551A1 (en) 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
JPH05184352A (ja) 1990-01-16 1993-07-27 Centro De Ing Genetica Y Biotecnol ピヒア パストリス(Pichia pastoris)酵母中での異種遺伝子の発現方法、発現ベクターおよび形質転換微生物
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
AU2008241061A1 (en) 2007-04-20 2008-10-30 Polymun Scientific Immunbiologische Forschung Gmbh Yeast expression systems
US7741075B2 (en) 2008-06-06 2010-06-22 Board Of Regents Of The University Of Nebraska Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display
WO2010135678A1 (en) 2009-05-22 2010-11-25 Research Corporation Technologies, Inc. Nucleic acids of pichia pastoris and use thereof for recombinant production of proteins
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DE102009055015B4 (de) 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
EP3590950A1 (en) 2011-05-09 2020-01-08 Ablynx NV Method for the production of immunoglobulin single varible domains
AU2013341049B2 (en) * 2012-10-29 2017-07-20 Lonza Ltd Expression sequences

Also Published As

Publication number Publication date
ES2610990T3 (es) 2017-05-04
JP6556625B2 (ja) 2019-08-07
PL2912053T3 (pl) 2017-05-31
CA2886460C (en) 2023-11-14
HUE033282T2 (en) 2017-11-28
CA2886460A1 (en) 2014-05-08
IL266700A (en) 2019-07-31
BR112015008289A2 (pt) 2017-11-28
EP3130598A3 (en) 2017-05-03
IL266700B (en) 2021-08-31
DK2912053T3 (en) 2017-01-30
CN104837857B (zh) 2018-11-06
US20180135092A1 (en) 2018-05-17
EP3130598B1 (en) 2020-02-12
US11359223B2 (en) 2022-06-14
JP2019141063A (ja) 2019-08-29
AU2013341049B2 (en) 2017-07-20
EP2912053A1 (en) 2015-09-02
KR20150076160A (ko) 2015-07-06
ES2789299T3 (es) 2020-10-26
WO2014067926A1 (en) 2014-05-08
HK1208037A1 (en) 2016-02-19
AU2013341049A1 (en) 2015-04-02
CN104837857A (zh) 2015-08-12
JP2015533286A (ja) 2015-11-24
CA3212565A1 (en) 2014-05-08
US10160988B2 (en) 2018-12-25
IN2015DN02771A (enExample) 2015-09-04
EP3130598A2 (en) 2017-02-15
SG11201502745XA (en) 2015-05-28
IL238420B (en) 2019-05-30
IL238420A0 (en) 2015-06-30
US20150232904A1 (en) 2015-08-20
EP2912053B1 (en) 2016-11-09

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