CA2877823A1 - Methods for nucleic acid assembly and high throughput sequencing - Google Patents
Methods for nucleic acid assembly and high throughput sequencing Download PDFInfo
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- CA2877823A1 CA2877823A1 CA2877823A CA2877823A CA2877823A1 CA 2877823 A1 CA2877823 A1 CA 2877823A1 CA 2877823 A CA2877823 A CA 2877823A CA 2877823 A CA2877823 A CA 2877823A CA 2877823 A1 CA2877823 A1 CA 2877823A1
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- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q2521/00—Reaction characterised by the enzymatic activity
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- 2013-06-24 WO PCT/US2013/047370 patent/WO2014004393A1/en not_active Ceased
- 2013-06-24 JP JP2015520370A patent/JP6509727B2/ja active Active
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| US20150191719A1 (en) | 2015-07-09 |
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| CN104685116A (zh) | 2015-06-03 |
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| CN113512577A (zh) | 2021-10-19 |
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| EP2864531A1 (en) | 2015-04-29 |
| US20200208139A1 (en) | 2020-07-02 |
| EP2864531A4 (en) | 2016-03-23 |
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| AU2021232763A1 (en) | 2021-11-18 |
| EP3483311A1 (en) | 2019-05-15 |
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| AU2019283944B2 (en) | 2021-07-08 |
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