CA2877823A1 - Methods for nucleic acid assembly and high throughput sequencing - Google Patents
Methods for nucleic acid assembly and high throughput sequencing Download PDFInfo
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- CA2877823A1 CA2877823A1 CA2877823A CA2877823A CA2877823A1 CA 2877823 A1 CA2877823 A1 CA 2877823A1 CA 2877823 A CA2877823 A CA 2877823A CA 2877823 A CA2877823 A CA 2877823A CA 2877823 A1 CA2877823 A1 CA 2877823A1
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- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
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- 2013-06-24 US US14/408,103 patent/US20150191719A1/en not_active Abandoned
- 2013-06-24 JP JP2015520370A patent/JP6509727B2/ja active Active
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| US12241057B2 (en) | 2025-03-04 |
| CN104685116A (zh) | 2015-06-03 |
| AU2017248573A1 (en) | 2017-11-09 |
| US20210395724A1 (en) | 2021-12-23 |
| AU2019283944A1 (en) | 2020-01-23 |
| JP7322202B2 (ja) | 2023-08-07 |
| EP3483311B1 (en) | 2025-09-17 |
| WO2014004393A1 (en) | 2014-01-03 |
| EP3483311B8 (en) | 2025-10-29 |
| EP2864531A4 (en) | 2016-03-23 |
| IL293796A (en) | 2022-08-01 |
| JP2015523866A (ja) | 2015-08-20 |
| EP2864531B2 (en) | 2022-08-03 |
| US20200208139A1 (en) | 2020-07-02 |
| EP3483311A1 (en) | 2019-05-15 |
| AU2019283944B2 (en) | 2021-07-08 |
| IL236303A0 (en) | 2015-02-26 |
| AU2021232763A1 (en) | 2021-11-18 |
| AU2013280661A1 (en) | 2015-01-22 |
| CN113512577A (zh) | 2021-10-19 |
| US20150191719A1 (en) | 2015-07-09 |
| JP2019150030A (ja) | 2019-09-12 |
| US11072789B2 (en) | 2021-07-27 |
| EP2864531B1 (en) | 2018-10-24 |
| EP2864531A1 (en) | 2015-04-29 |
| IL236303B (en) | 2022-07-01 |
| LT2864531T (lt) | 2019-03-12 |
| JP2022050592A (ja) | 2022-03-30 |
| JP7009409B2 (ja) | 2022-01-25 |
| JP6509727B2 (ja) | 2019-05-15 |
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