JP2008539759A - 多数の温度で生化学的又は化学的な反応を実施する方法及び装置 - Google Patents
多数の温度で生化学的又は化学的な反応を実施する方法及び装置 Download PDFInfo
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Abstract
Description
装置は、更に、他の反応ゾーンとは異なる温度を維持できる反応ゾーンであって、少なくとも2つの反応ゾーンを備えており、反応通路が、少なくとも2つの反応ゾーンを通っており、エレクトロウェッティングアレイが、反応通路、検出部位、及び戻り通路を通して、反応液滴を操作可能である。
一実施形態において、装置は、多数のエレクトロウェッティング電極を備えた、エレクトロウェッティングアレイを、備えており、方法は、反応を実施するために、1つ以上の反応液滴を、エレクトロウェッティングを用いて、異なる温度を有するエレクトロウェッティングアレイ上の、種々の反応ゾーンを通して移動させる工程を、含んでいる。
装置の反応ゾーンを通して、反応液滴を作動させるために、エレクトロウェッティングアレイ及び電極を用いるのに加えて、他の作動手段が、ここに記載された装置及び方法を用いて、使用されてもよい。すなわち、反応液滴又は反応体積を作動させるための、あらゆる機構が、ここに記載された装置及び方法において、用いられてもよく、その装置及び方法は、熱アクチュエータと、バブルベースアクチュエータと、マイクロバルブベースアクチュエータと、を含んでいるが、これらに限定されない。エレクトロウェッティングが、反応を実施するために液体を操作するのに使用されているという、装置及び方法のこの記述は、他の作動手段を使用する装置及び方法に、等しく、適用可能である。
Claims (13)
- 異なる温度を必要とする核酸増幅反応を実施する方法であって、
(a)少なくとも1つの反応液滴を、少なくとも2つの反応ゾーンを備えたエレクトロウェッティングアレイに供給する、工程;これにおいて、各反応ゾーンは、核酸増幅反応に必要とされる異なる温度を有しており、反応液滴は、標的核酸と、核酸の増幅を生じさせるのに必要とされる試薬と、を含んでいる、
(b)核酸増幅反応の初回サイクルが完了するように、少なくとも1つの反応液滴を、エレクトロウェッティングを用いて、少なくとも2つの反応ゾーンを通して移動させることによって、核酸増幅反応を実施する、工程;
(c)核酸増幅反応の更なるサイクルを実施するために、工程(b)を任意に繰り返す、工程;
を有することを特徴とする、方法。 - 標的核酸を増幅させる方法であって、
(a)少なくとも1つの反応液滴をエレクトロウェッティングアレイに供給する、工程;これにおいて、反応液滴は、標的核酸と、核酸の増幅を生じさせるのに必要とされる試薬と、を含んでおり、試薬は、核酸プライマーを含んでいる、
(b)標的核酸が変性されるように、液滴を、エレクトロウェッティングを用いて、エレクトロウェッティングアレイの第1温度を有する第1反応ゾーンを通して移動させる、工程;
(c)プライマーが標的核酸にアニールされるように、液滴を、エレクトロウェッティングを用いて、エレクトロウェッティングアレイの第2温度を有する第2反応ゾーンを通して移動させる、工程;
(d)核酸プライマーの伸長が生じ、それによって、標的核酸が増幅するように、液滴を、エレクトロウェッティングを用いて、エレクトロウェッティングアレイの第3温度を有する第3反応ゾーンを通して移動させる、工程;
工程(b)、(c)、(d)を任意に繰り返す、工程;
を有することを特徴とする、方法。 - (e)第3反応ゾーンから検出部位へ、エレクトロウェッティングを用いて、液滴を移動させる、工程;
(f)反応液滴中の増幅された核酸の存在を検出する、工程;
を更に有する、請求項2記載の方法。 - 反応液滴を、検出部位から、エレクトロウェッティングアレイの戻り通路に沿って、第1反応ゾーンへ、移動させ、工程(b)、(c)、(d)を繰り返す、工程;
を更に有する、請求項3記載の方法。 - 標的核酸を増幅させる方法であって、
(a)少なくとも1つの反応液滴をエレクトロウェッティングアレイに供給する、工程;これにおいて、反応液滴は、標的核酸と、核酸の増幅を生じさせるのに必要とされる試薬と、を含んでおり、試薬は、核酸プライマーを含んでいる、
(b)標的核酸が変性されるように、液滴を、エレクトロウェッティングを用いて、エレクトロウェッティングアレイの第1温度を有する第1反応ゾーンを通して移動させる、工程;
(c)プライマーが標的核酸にアニールされるように、且つ、核酸プライマーの伸長が生じ、それによって、標的核酸が増幅するように、液滴を、エレクトロウェッティングを用いて、エレクトロウェッティングアレイの第2温度を有する第2反応ゾーンを通して移動させる、工程;
工程(b)、(c)を任意に繰り返す、工程;
を有することを特徴とする、方法。 - 種々の温度で化学的又は生化学的な反応を実施する装置であって、
少なくとも1つの反応通路と、少なくとも1つの検出部位と、少なくとも1つの戻り通路と、を備えた、マイクロフルイディクス装置と、
反応液滴又は反応体積を、反応通路、検出部位、及び戻り通路を通して、作動させる、手段と、
他の反応ゾーンとは異なる温度を維持できる反応ゾーンであって、少なくとも2つの反応ゾーンと、
を備えており、
反応通路が、少なくとも2つの反応ゾーンを通っていることを特徴とする、装置。 - 反応通路の少なくとも1つと、検出部位の少なくとも1つと、戻り通路の少なくとも1つとが、流体連結されているループを形成している、請求項6記載の装置。
- 反応液滴又は反応体積を作動させる手段が、1つ以上のエレクトロウェッティング電極、熱アクチュエータ、バブルベースアクチュエータ、又はマイクロバルブベースアクチュエータを、備えている、請求項6記載の装置。
- 種々の温度で化学的又は生化学的な反応を実施する装置であって、
複数の反応通路と、少なくとも1つの検出部位と、少なくとも1つの戻り通路と、を備えた、マイクロフルイディクス装置と、
反応液滴又は反応体積を、反応通路、検出部位、及び戻り通路を通して、作動させる、手段と、
他の反応ゾーンとは異なる温度を維持できる反応ゾーンであって、少なくとも2つの反応ゾーンと、
を備えており、
反応通路のそれぞれが、少なくとも2つの反応ゾーンを通っており、
反応通路の少なくとも1つが、少なくとも1つの検出部位に、流体連結していることを特徴とする、装置。 - 種々の温度で化学的又は生化学的な反応を実施する装置であって、
少なくとも1つの反応通路と少なくとも1つの検出部位と少なくとも1つの戻り通路とを形成する、複数のエレクトロウェッティング電極を、備えたエレクトロウェッティングアレイと、
他の反応ゾーンとは異なる温度を維持できる反応ゾーンであって、少なくとも2つの反応ゾーンと、
を備えており、
反応通路が、少なくとも2つの反応ゾーンを通っており、
エレクトロウェッティングアレイが、反応通路、検出部位、及び戻り通路を通して、反応液滴を操作可能であることを特徴とする、装置。 - 各反応通路が、少なくとも1つの検出部位に隣接している、請求項10記載の装置。
- 異なる温度を必要とする反応を実施する方法であって、
(a)少なくとも1つの反応液滴を、少なくとも2つの反応ゾーンを備えたエレクトロウェッティングアレイに供給する、工程;これにおいて、各反応ゾーンは、反応に必要とされる異なる温度を有しており、反応液滴は、反応を生じさせるのに必要とされる試薬を含んでいる、
(b)反応の初回サイクルが完了するように、少なくとも1つの反応液滴を、エレクトロウェッティングを用いて、少なくとも2つの反応ゾーンを通して移動させることによって、反応を実施する、工程;
(c)反応の更なるサイクルを実施するために、工程(b)を任意に繰り返す、工程;
を有することを特徴とする、方法。 - 異なる温度を必要とする反応を実施する方法であって、
(a)少なくとも1つの反応液滴又は反応体積を、少なくとも2つの反応ゾーン及び少なくとも1つの検出部位を備えたマイクロフルイディクス装置に、供給する、工程;これにおいて、各反応ゾーンは、反応に必要とされる異なる温度を有しており、反応液滴は、反応を生じさせるのに必要とされる試薬を含んでいる、
(b)反応の初回サイクルが完了するように、少なくとも1つの反応液滴又は反応体積を、作動手段を用いて、少なくとも2つの反応ゾーンを通して移動させることによって、反応を実施する、工程;
(c)反応の更なるサイクルを実施するために、工程(b)を任意に繰り返す、工程;
を有することを特徴とする、方法。
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JP2013508129A (ja) * | 2009-10-15 | 2013-03-07 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 放射化学のためのデジタル微小流体プラットフォーム |
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AU2006247752A1 (en) | 2006-11-23 |
US20080274513A1 (en) | 2008-11-06 |
EP1885885A2 (en) | 2008-02-13 |
US9452433B2 (en) | 2016-09-27 |
WO2006124458A3 (en) | 2007-11-29 |
EP1885885A4 (en) | 2008-08-27 |
KR101431775B1 (ko) | 2014-08-20 |
AU2006247752B2 (en) | 2012-04-12 |
US20140329307A1 (en) | 2014-11-06 |
CA2606750C (en) | 2015-11-24 |
JP2013172724A (ja) | 2013-09-05 |
CN101287845A (zh) | 2008-10-15 |
US9216415B2 (en) | 2015-12-22 |
US20120132528A1 (en) | 2012-05-31 |
KR20080011318A (ko) | 2008-02-01 |
WO2006124458A2 (en) | 2006-11-23 |
CA2606750A1 (en) | 2006-11-23 |
US9517469B2 (en) | 2016-12-13 |
CN101287845B (zh) | 2012-07-18 |
US20170080428A1 (en) | 2017-03-23 |
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