CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents

Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDF

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Publication number
CA2876178C
CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
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CA
Canada
Prior art keywords
microorganism
biodiesel
clostridium
nucleic acid
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2876178A
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English (en)
French (fr)
Other versions
CA2876178A1 (en
Inventor
Fungmin Liew
Michael Koepke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzatech NZ Inc
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49769067&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2876178(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of CA2876178A1 publication Critical patent/CA2876178A1/en
Application granted granted Critical
Publication of CA2876178C publication Critical patent/CA2876178C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2876178A 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Active CA2876178C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261662467P 2012-06-21 2012-06-21
US61/662,467 2012-06-21
PCT/NZ2013/000108 WO2013191567A1 (en) 2012-06-21 2013-06-21 Recombinant microorganisms make biodiesel

Publications (2)

Publication Number Publication Date
CA2876178A1 CA2876178A1 (en) 2013-12-27
CA2876178C true CA2876178C (en) 2017-02-28

Family

ID=49769067

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2876178A Active CA2876178C (en) 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel

Country Status (13)

Country Link
US (1) US9347076B2 (oth)
EP (1) EP2864470B2 (oth)
JP (1) JP6466836B2 (oth)
KR (1) KR102097844B1 (oth)
CN (1) CN104640977B (oth)
BR (1) BR112014031876B1 (oth)
CA (1) CA2876178C (oth)
ES (1) ES2655214T5 (oth)
IN (1) IN2014DN10507A (oth)
MY (1) MY164882A (oth)
NO (1) NO2953671T3 (oth)
PL (1) PL2864470T5 (oth)
WO (1) WO2013191567A1 (oth)

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EP4162056A4 (en) 2020-06-06 2024-05-29 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
MY201959A (en) 2021-02-08 2024-03-26 Lanzatech Inc Recombinant microorganisms and uses therefor
CN117098850A (zh) 2021-04-09 2023-11-21 朗泽科技有限公司 用于提供原料的方法和设备
WO2023004293A1 (en) 2021-07-20 2023-01-26 Lanzatech, Inc. Recombinant microorganisms and uses therefor
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Also Published As

Publication number Publication date
BR112014031876A2 (pt) 2017-08-01
EP2864470B2 (en) 2020-12-23
CA2876178A1 (en) 2013-12-27
ES2655214T5 (es) 2021-10-11
JP2015519918A (ja) 2015-07-16
MY164882A (en) 2018-01-30
ES2655214T3 (es) 2018-02-19
JP6466836B2 (ja) 2019-02-06
KR102097844B1 (ko) 2020-04-06
CN104640977B (zh) 2019-04-12
US20130344547A1 (en) 2013-12-26
EP2864470B1 (en) 2017-10-04
WO2013191567A1 (en) 2013-12-27
EP2864470A1 (en) 2015-04-29
PL2864470T5 (pl) 2021-05-17
IN2014DN10507A (oth) 2015-08-21
US9347076B2 (en) 2016-05-24
EP2864470A4 (en) 2015-12-16
KR20150023651A (ko) 2015-03-05
BR112014031876B1 (pt) 2022-05-10
NO2953671T3 (oth) 2018-03-17
PL2864470T3 (pl) 2018-05-30
CN104640977A (zh) 2015-05-20

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