CA2842377C - Procede et systeme de detection de cellules cancereuses et/ou de classification de cellules dans un echantillon de cellules - Google Patents
Procede et systeme de detection de cellules cancereuses et/ou de classification de cellules dans un echantillon de cellules Download PDFInfo
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Abstract
La présente invention concerne un procédé de détection de cellules cancéreuses et/ou de classification de cellules dans un échantillon de cellules. Ce procédé comporte les étapes suivantes: réalisation d'un échantillon de cellules; obtention d'information holographique provenant dudit échantillon de cellules, par microscopie holographique numérique ou "DMH" (Digital Holographic Microscopy), dérivant de ladite information holographique au moins un paramètre cellulaire; et classification desdites cellules de l'échantillon de cellules. Ce procédé est caractérisé en ce que ladite classification se fait par affectation d'un facteur de notation auxdites cellules de l'échantillon de cellules, sur la base des paramètres cellulaires considérés. L'invention concerne également, dans un second aspect, un système, qui est destiné à la détection de cellules cancéreuses et/ou à la classification de cellules dans un échantillon de cellules, et qui met en uvre le procédé de l'invention. Dans un dernier aspect, l'invention concerne aussi un procédé de mise à jour et/ou d'amélioration d'une base de données comprenant des seuils reliés à l'information holographique et à la base de données qui s'y rapporte.
Applications Claiming Priority (5)
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EP11174583 | 2011-07-19 | ||
EP11174583.2 | 2011-07-19 | ||
EP2011070571 | 2011-11-21 | ||
EPPCT/EP2011/070571 | 2011-11-21 | ||
PCT/EP2012/063936 WO2013011001A1 (fr) | 2011-07-19 | 2012-07-16 | Procédé et système de détection de cellules cancéreuses et/ou de classification de cellules dans un échantillon de cellules |
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CA2842377A1 CA2842377A1 (fr) | 2013-01-24 |
CA2842377C true CA2842377C (fr) | 2019-08-27 |
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CA2842377A Active CA2842377C (fr) | 2011-07-19 | 2012-07-16 | Procede et systeme de detection de cellules cancereuses et/ou de classification de cellules dans un echantillon de cellules |
Country Status (8)
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US (2) | US9684281B2 (fr) |
EP (1) | EP2734955A1 (fr) |
JP (1) | JP6092206B2 (fr) |
KR (2) | KR20160103175A (fr) |
CN (2) | CN108107197B (fr) |
BR (1) | BR112014001238A2 (fr) |
CA (1) | CA2842377C (fr) |
WO (1) | WO2013011001A1 (fr) |
Families Citing this family (68)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8891851B2 (en) * | 2009-07-15 | 2014-11-18 | Glenn F. Spaulding | Home healthcare management system and hardware |
US9522396B2 (en) | 2010-12-29 | 2016-12-20 | S.D. Sight Diagnostics Ltd. | Apparatus and method for automatic detection of pathogens |
BR112014001238A2 (pt) * | 2011-07-19 | 2017-06-13 | Ovizio Imaging Systems N V | método para a detecção de células cancerosas e/ou classificação de células numa amostra de células de líquido; sistema para a detecção de células e/ou a classificação de células cancerosas numa amostra de células; método de atualização e/ou melhoria de uma base de dados que compreende os limiares ligados á informação holográfica; e base de dados de objetos |
EP2594334A1 (fr) | 2011-11-21 | 2013-05-22 | Drive O2 | Fiole à échantillon pour analyse holographique numérique d'un échantillon de cellules liquides |
BR112014016072B1 (pt) | 2011-12-29 | 2021-01-12 | Sight Diagnostics Ltd. | método e sistema para detectar uma infecção por plasmodium em uma amostra de sangue |
EP2626686A1 (fr) | 2012-02-13 | 2013-08-14 | Ovizio Imaging Systems NV/SA | Cytomètre de flux avec microscope holographique numérique |
EP2898310B1 (fr) | 2012-09-20 | 2019-05-01 | Ovizio Imaging Systems NV/SA | Microscope holographique numérique ayant des systèmes de fluide |
ES2845600T3 (es) | 2013-01-09 | 2021-07-27 | Univ California | Aparato y métodos para la obtención de imágenes de fluorescencia utilizando excitación multiplexada por radiofrecuencia |
EP2999988A4 (fr) | 2013-05-23 | 2017-01-11 | S.D. Sight Diagnostics Ltd. | Procédé et système d'imagerie de prélèvement cellulaire |
IL227276A0 (en) | 2013-07-01 | 2014-03-06 | Parasight Ltd | A method and system for obtaining a monolayer of cells, for use specifically for diagnosis |
EP3039477B1 (fr) | 2013-08-26 | 2021-10-20 | S.D. Sight Diagnostics Ltd. | Systèmes, procédés et produits programmes d'ordinateur de microscopie numérique |
DE102014200911A1 (de) * | 2013-10-09 | 2015-04-09 | Siemens Aktiengesellschaft | In-Vitro-Verfahren zum markierungsfreien Bestimmen eines Zelltyps einer Zelle |
US9430829B2 (en) * | 2014-01-30 | 2016-08-30 | Case Western Reserve University | Automatic detection of mitosis using handcrafted and convolutional neural network features |
ES2959506T3 (es) | 2014-03-18 | 2024-02-26 | Univ California | Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método |
DE102014205535A1 (de) * | 2014-03-25 | 2015-10-01 | Siemens Aktiengesellschaft | Vorrichtung und in-vitro Verfahren zur markierungsfreien Darstellung und Analyse von Zellen und Kristallen in einer biologischen Probe |
WO2015195642A1 (fr) * | 2014-06-16 | 2015-12-23 | Siemens Healthcare Diagnostics Inc. | Analyse de données de microscopie holographique numérique pour des applications d'hématologie |
US10614287B2 (en) | 2014-06-16 | 2020-04-07 | Siemens Healthcare Diagnostics Inc. | Virtual staining of cells in digital holographic microscopy images using general adversarial networks |
WO2016030897A1 (fr) | 2014-08-27 | 2016-03-03 | S.D. Sight Diagnostics Ltd | Système et procédé de calcul de variation de focalisation pour un microscope numérique |
FR3027107B1 (fr) * | 2014-10-09 | 2019-09-13 | Espci Paristech | Methode et dispositif de detection optique de nanoparticules dans un echantillon fluide |
FR3030749B1 (fr) * | 2014-12-19 | 2020-01-03 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Methode d'identification de particules biologiques par piles d'images holographiques defocalisees |
FR3034196B1 (fr) * | 2015-03-24 | 2019-05-31 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Procede d'analyse de particules |
US10304188B1 (en) | 2015-03-27 | 2019-05-28 | Caleb J. Kumar | Apparatus and method for automated cell analysis |
BR112017021318A2 (pt) * | 2015-04-09 | 2018-06-26 | Koninklijke Philips N.V. | método implementado por computador, e mídia legível por computador |
AU2016322966B2 (en) | 2015-09-17 | 2021-10-14 | S.D. Sight Diagnostics Ltd | Methods and apparatus for detecting an entity in a bodily sample |
US10078045B2 (en) | 2015-10-13 | 2018-09-18 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
EP3159690A1 (fr) | 2015-10-22 | 2017-04-26 | Universität Basel | Profilage nanomécanique de sous-types moléculaire de cancer du sein |
CN108603876A (zh) * | 2015-12-30 | 2018-09-28 | 维森盖特有限公司 | 用于自动检测和监测发育异常和施用化学预防的系统和方法 |
EP3196631A1 (fr) | 2016-01-19 | 2017-07-26 | Ovizio Imaging Systems NV/SA | Microscope holographique numérique avec système électrofluidique, ledit système électrofluidique et procédés d'utilisation |
CA3018065A1 (fr) | 2016-03-17 | 2017-09-21 | Bd Biosciences | Tri de cellules a l'aide d'un cytometre de flux a fluorescence a haut debit |
EP3436864B1 (fr) | 2016-03-30 | 2021-04-28 | S.D. Sight Diagnostics Ltd. | Dispositif de traitement d'image pour l'identification des parasites du sang |
CA3022770A1 (fr) | 2016-05-11 | 2017-11-16 | S.D. Sight Diagnostics Ltd | Conduite de mesures optiques sur un echantillon |
EP3455610B1 (fr) | 2016-05-11 | 2023-01-04 | S.D. Sight Diagnostics Ltd. | Porte-échantillon pour mesures optiques |
EP3252455A1 (fr) * | 2016-05-30 | 2017-12-06 | Biomérieux | Dispositif et procede d'acquisition d'une particule presente dans un echantillon |
EP3519828A4 (fr) | 2016-09-29 | 2020-06-03 | Animantis, LLC | Procédés et appareil d'évaluation de l'activité du système immunitaire et de l'efficacité thérapeutique |
JP7130630B2 (ja) * | 2016-09-30 | 2022-09-05 | モレキュラー デバイシーズ, エルエルシー | 最適候補化合物を検出するためのコンピュータ装置およびその方法 |
CN108537092B (zh) * | 2017-03-02 | 2020-11-10 | 爱威科技股份有限公司 | 变异红细胞识别方法及装置 |
JPWO2018207361A1 (ja) * | 2017-05-12 | 2020-03-12 | オリンパス株式会社 | 細胞画像取得装置 |
US9870615B2 (en) | 2017-07-19 | 2018-01-16 | Schwalb Consulting, LLC | Morphology identification in tissue samples based on comparison to named feature vectors |
CN109697450B (zh) * | 2017-10-20 | 2023-04-07 | 曦医生技股份有限公司 | 细胞分类方法 |
TWI637146B (zh) * | 2017-10-20 | 2018-10-01 | 曦醫生技股份有限公司 | Cell classification method |
WO2019089855A1 (fr) | 2017-11-01 | 2019-05-09 | Juno Therapeutics, Inc. | Procédé de génération de compositions thérapeutiques de cellules modifiées |
CA3081669A1 (fr) | 2017-11-14 | 2019-05-23 | S.D. Sight Diagnostics Ltd | Porte-echantillon pour mesures optiques |
SG11202005272SA (en) | 2017-12-08 | 2020-07-29 | Juno Therapeutics Inc | Process for producing a composition of engineered t cells |
CN108364266B (zh) * | 2018-02-09 | 2021-11-30 | 重庆大学 | 使用微流控芯片进行细胞全息重建观测的流路结构去除方法 |
WO2019176427A1 (fr) * | 2018-03-12 | 2019-09-19 | 富士フイルム株式会社 | Procédé de détermination |
BE1026111B1 (nl) * | 2018-03-15 | 2019-10-16 | Ovizio Imaging Systems Nv | Digitale holografische microscopie voor het bepalen van de status van een virale infectie |
CN116088158A (zh) * | 2018-07-13 | 2023-05-09 | 深圳迈瑞生物医疗电子股份有限公司 | 一种细胞图像处理系统、方法、自动读片装置与存储介质 |
EP3859006B1 (fr) | 2018-09-28 | 2024-07-03 | FUJIFILM Corporation | Procédé de détermination |
CN113227358A (zh) | 2018-10-31 | 2021-08-06 | 朱诺治疗学有限公司 | 选择并刺激细胞的方法及用于所述方法的设备 |
JP7392411B2 (ja) * | 2018-11-16 | 2023-12-06 | ソニーグループ株式会社 | 情報処理装置、情報処理方法及びプログラム |
CN111337696B (zh) * | 2018-12-18 | 2024-03-29 | 深圳迈瑞生物医疗电子股份有限公司 | 一种体外诊断设备及其样本分析方法 |
US20200200671A1 (en) * | 2018-12-20 | 2020-06-25 | Sony Corporation | Information processing apparatus, information processing method, and program |
US20220156561A1 (en) * | 2019-03-13 | 2022-05-19 | Tomocube, Inc. | Identifying microorganisms using three-dimensional quantitative phase imaging |
CN110060729B (zh) * | 2019-03-28 | 2020-02-28 | 广州序科码生物技术有限责任公司 | 一种基于单细胞转录组聚类结果注释细胞身份的方法 |
EP3997439A4 (fr) | 2019-07-10 | 2023-07-19 | Becton, Dickinson and Company | Circuits intégrés reconfigurables pour ajuster une classification de tri de cellules |
CN112305245B (zh) * | 2019-07-30 | 2024-04-19 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析方法及样本分析系统 |
JP2022546396A (ja) * | 2019-08-30 | 2022-11-04 | ジュノー セラピューティクス インコーポレイテッド | 細胞を分類するための機械学習方法 |
US20220412954A1 (en) | 2019-11-05 | 2022-12-29 | Juno Therapeutics, Inc. | Methods of determining attributes of therapeutic t cell compositions |
EP4073566A1 (fr) | 2019-12-12 | 2022-10-19 | S.D. Sight Diagnostics Ltd. | Génération artificielle d'image couleur de frottis sanguin |
WO2021205483A1 (fr) * | 2020-04-08 | 2021-10-14 | Alifax S.R.L. | Méthode et système de détection et de classification des bactéries et autres cellules dans un échantillon d'urine |
CN111524132B (zh) * | 2020-05-09 | 2022-10-18 | 腾讯医疗健康(深圳)有限公司 | 识别待检测样本中异常细胞的方法、装置和存储介质 |
EP4150640A1 (fr) | 2020-05-13 | 2023-03-22 | Juno Therapeutics, Inc. | Procédés d'identification de caractéristiques associées à une réponse clinique et leurs utilisations |
KR102639140B1 (ko) | 2020-08-14 | 2024-02-20 | 연세대학교 산학협력단 | 조직 검사 방법 및 조직 검사 장치 |
US11499815B2 (en) | 2020-12-08 | 2022-11-15 | International Business Machines Corporation | Visual quality assessment augmentation employing holographic interferometry |
JP2024511418A (ja) | 2021-03-22 | 2024-03-13 | ジュノー セラピューティクス インコーポレイテッド | 治療用細胞組成物の効力を決定する方法 |
CN113447410B (zh) * | 2021-06-24 | 2022-12-23 | 桂林理工大学 | 低空无人机探测地面火灾的方法及系统 |
WO2023230548A1 (fr) | 2022-05-25 | 2023-11-30 | Celgene Corporation | Procédé de prédiction d'une réponse à une thérapie par lymphocyte t |
WO2024124132A1 (fr) | 2022-12-09 | 2024-06-13 | Juno Therapeutics, Inc. | Procédés d'apprentissage automatique pour prédire un phénotype cellulaire au moyen d'une imagerie holographique |
Family Cites Families (87)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5089416A (en) | 1984-12-24 | 1992-02-18 | Caribbean Microparticles Corporation | Method of use of non-fluorescent particles to determine fluorescence threshold of a flow cytometer relative to the autofluorescence of samples |
US4786594A (en) | 1986-05-14 | 1988-11-22 | Syntex (U.S.A.) Inc. | Enzyme immunoassay |
US5740270A (en) * | 1988-04-08 | 1998-04-14 | Neuromedical Systems, Inc. | Automated cytological specimen classification system and method |
FR2642522B1 (fr) | 1989-01-31 | 1991-05-10 | Elf Aquitaine | Appareil de mesure de densite optique in situ pour fermenteur |
DE69118295T2 (de) | 1990-10-01 | 1996-09-19 | Canon Kk | Vorrichtung und Verfahren zur Messung einer Probe |
US5256571A (en) | 1991-05-01 | 1993-10-26 | Cytyc Corporation | Cell preservative solution |
US5495333A (en) | 1992-07-24 | 1996-02-27 | Sumitomo Electric Industries, Ltd. | Method and apparatus of detecting impurities in fluid |
US6026174A (en) * | 1992-10-14 | 2000-02-15 | Accumed International, Inc. | System and method for automatically detecting malignant cells and cells having malignancy-associated changes |
US5733721A (en) * | 1992-11-20 | 1998-03-31 | The Board Of Regents Of The University Of Oklahoma | Cell analysis method using quantitative fluorescence image analysis |
US6924094B1 (en) | 1996-02-08 | 2005-08-02 | Affymetrix, Inc. | Chip-based species identification and phenotypic characterization of microorganisms |
CA2293426C (fr) | 1997-06-09 | 2007-05-15 | Guava Technologies, Inc. | Procede et dispositif pour deceler des microparticules dans des echantillons de fluides |
GB2330516A (en) | 1997-10-22 | 1999-04-28 | Elizabeth Acton | Cryopreservation of cell suspensions |
US6235536B1 (en) | 1998-03-07 | 2001-05-22 | Robert A. Levine | Analysis of quiescent anticoagulated whole blood samples |
DE19826470C2 (de) | 1998-06-13 | 2001-10-18 | Eppendorf Ag | Küvettensystem und Küvette |
US8131053B2 (en) | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US6574401B2 (en) | 1999-03-30 | 2003-06-03 | Ceramoptec Industries, Inc. | Optical fiber-handpiece combination for medical laser treatments |
US6651008B1 (en) | 1999-05-14 | 2003-11-18 | Cytokinetics, Inc. | Database system including computer code for predictive cellular bioinformatics |
DE10016838B4 (de) | 2000-04-05 | 2006-10-19 | Jan-Gerd Dipl.-Ing. Frerichs | In-situ Mikroskopvorrichtung für Reaktoren |
US6394966B1 (en) | 2000-09-08 | 2002-05-28 | Diagnostic Cytology Laboratories, Inc. | Apparatus and method for removing cells from an endocervical brush in a liquid-based pap test system |
US20020164825A1 (en) | 2000-09-09 | 2002-11-07 | Wen-Tien Chen | Cell separation matrix |
US7027633B2 (en) | 2000-11-30 | 2006-04-11 | Foran David J | Collaborative diagnostic systems |
US7050620B2 (en) * | 2001-03-30 | 2006-05-23 | Heckman Carol A | Method of assaying shape and structural features in cells |
US6965793B2 (en) | 2001-06-28 | 2005-11-15 | Chemimage Corporation | Method for Raman chemical imaging of endogenous chemicals to reveal tissue lesion boundaries in tissue |
WO2003002972A2 (fr) | 2001-06-29 | 2003-01-09 | Universite Libre De Bruxelles | Procede et dispositif destines a l'obtention par microscopie d'images en trois dimensions d'un echantillon |
US6943924B2 (en) | 2001-12-04 | 2005-09-13 | Ecole Polytechnique Federale De Lausanne (Epfl) | Apparatus and method for digital holographic imaging |
US20030113832A1 (en) | 2001-12-14 | 2003-06-19 | Lauf Robert J. | Apparatus and method for assaying electrophysiological effects |
US20030199649A1 (en) | 2002-03-26 | 2003-10-23 | Orbison David Robert | Method and apparatus for the controlled dilution of organometallic compounds |
GB0215878D0 (en) * | 2002-07-09 | 2002-08-14 | Smart Holograms Ltd | Cell detection |
DE10237082B4 (de) | 2002-08-09 | 2014-12-31 | Sartorius Stedim Biotech Gmbh | Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen |
US20040123234A1 (en) | 2002-12-18 | 2004-06-24 | Schemalogic, Inc. | Method and system for change control management of shema definition objects |
EP1524491A1 (fr) | 2003-10-16 | 2005-04-20 | Universite Libre De Bruxelles | Appareil associant un interféromètre et un microscope |
PL1631788T3 (pl) | 2003-05-16 | 2007-08-31 | Univ Bruxelles | Cyfrowy mikroskop holograficzny do trójwymiarowego obrazowania i sposób jego stosowania |
US20070065810A1 (en) | 2003-07-18 | 2007-03-22 | Georgetown University | Diagnosis and treatment of cervical cancer |
US7286222B2 (en) | 2003-07-18 | 2007-10-23 | Chemimage Corporation | Sample container and system for a handheld spectrometer and method for using therefor |
US20060088814A1 (en) | 2004-10-26 | 2006-04-27 | Cytyc Corporation | Enhanced cell preservative solution and methods for using same |
WO2006047252A1 (fr) | 2004-10-26 | 2006-05-04 | Cytyc Corporation | Solution de conservation de cellules amelioree, et methodes d'utilisation associees |
US7275682B2 (en) | 2005-03-24 | 2007-10-02 | Varian, Inc. | Sample identification utilizing RFID tags |
CN101151517A (zh) * | 2005-03-29 | 2008-03-26 | 希森美康株式会社 | 鉴别癌症/异型细胞和粒子团的方法及细胞分析仪 |
EP1731099A1 (fr) | 2005-06-06 | 2006-12-13 | Paul Scherrer Institut | Interféromètre pour l'imagerie et la tomographie à contraste de phase avec une source de rayons X incohérente et polychromatique |
WO2007002898A2 (fr) * | 2005-06-29 | 2007-01-04 | University Of South Florida | Balayage tomographique variable avec holographie interferentielle numerique a balayage en longueur d'onde |
US8149416B2 (en) * | 2005-10-17 | 2012-04-03 | Arryx, Inc. | Apparatus and method for dynamic cellular probing and diagnostics using holographic optical forcing array |
US8249326B2 (en) * | 2005-11-25 | 2012-08-21 | British Columbia Cancer Agency Branch | Apparatus and methods for automated assessment of tissue pathology |
EP1963927B1 (fr) | 2005-12-22 | 2020-01-15 | Phase Holographic Imaging PHI AB | Procede et dispositif d'analyse d'un echantillon de cellules |
US7651869B2 (en) | 2006-03-14 | 2010-01-26 | Research International, Inc. | Optical assay apparatus and methods |
US7616320B2 (en) * | 2006-03-15 | 2009-11-10 | Bahram Javidi | Method and apparatus for recognition of microorganisms using holographic microscopy |
CA2650041A1 (fr) | 2006-04-21 | 2007-11-01 | Klaus Michael Joeris | Systeme et methode de mesures sur place |
US8428331B2 (en) * | 2006-08-07 | 2013-04-23 | Northeastern University | Phase subtraction cell counting method |
US8865162B2 (en) | 2008-06-13 | 2014-10-21 | Oncohealth Corp. | Monoclonal antibodies against HPV proteins |
AU2007334343A1 (en) * | 2006-12-15 | 2008-06-26 | Ordway Research Institute, Inc. | Treatments of therapy-resistant diseases comprising drug combinations |
US7478008B2 (en) * | 2007-03-16 | 2009-01-13 | Cordis Corporation | System and method for the non-destructive assessment of the quantitative spatial distribution of components of a medical device |
CA2964611C (fr) | 2007-03-28 | 2021-06-01 | Bionano Genomics, Inc. | Procedes d'analyse macromoleculaire utilisant des reseaux de nanocanaux |
GB0707776D0 (en) | 2007-04-23 | 2007-05-30 | Robio Systems Ltd | A Container for culturing and/or transporting embryos or oocytes. an insert for the container and a method of transporting same |
US20080306200A1 (en) * | 2007-06-11 | 2008-12-11 | Seong Fong Chen | Antistatic gloves and process for making same |
US8785180B2 (en) | 2007-06-18 | 2014-07-22 | Shanghai Guoqiang Bioengineering Equipment Co., Ltd. | Biochemical reactor |
EP2263797B1 (fr) | 2007-06-25 | 2011-09-07 | ibidi GmbH | Chambre d'analyse |
US20090082637A1 (en) * | 2007-09-21 | 2009-03-26 | Michael Galperin | Multi-modality fusion classifier with integrated non-imaging factors |
CN101971188B (zh) | 2007-10-16 | 2013-10-30 | 狄克雷佩特公司 | 处理上位化学结构表征的方法 |
JP5378228B2 (ja) * | 2007-10-29 | 2013-12-25 | シスメックス株式会社 | 細胞分析装置及び細胞分析方法 |
US8791985B2 (en) * | 2007-10-30 | 2014-07-29 | New York University | Tracking and characterizing particles with holographic video microscopy |
US8049814B2 (en) * | 2008-03-27 | 2011-11-01 | The Rockefeller University | Holographic microscope |
JP2011525252A (ja) | 2008-06-19 | 2011-09-15 | フェイズ ホログラフィック イメージング ペーホーイー アーベー | 透明な生体の分析 |
GB2474613A (en) * | 2008-07-10 | 2011-04-20 | Nodality Inc | Methods and apparatus related to management of experiments |
JP2010057902A (ja) | 2008-08-06 | 2010-03-18 | Toshiba Corp | レポート作成支援装置、レポート作成支援システム、及び医用画像参照装置 |
JP2012504956A (ja) | 2008-10-10 | 2012-03-01 | セントレ ナショナル デ ラ レシェルシェ サイエンティフィーク−ディーエーイー | 細胞ソート・デバイス |
US8599383B2 (en) | 2009-05-06 | 2013-12-03 | The Regents Of The University Of California | Optical cytometry |
WO2010148152A2 (fr) | 2009-06-16 | 2010-12-23 | Ikonisys, Inc. | Système et procédé de commande à distance d'un microscope |
US8885035B2 (en) | 2009-06-16 | 2014-11-11 | Lester F. Ludwig | Electronic imaging flow-microscope for environmental remote sensing, bioreactor process monitoring, and optical microscopic tomography |
JP5454875B2 (ja) * | 2009-06-25 | 2014-03-26 | 株式会社東芝 | 画像診断支援システム |
US8610085B2 (en) | 2009-08-20 | 2013-12-17 | Bio-Rad Laboratories, Inc. | High-speed cellular cross sectional imaging |
BR112012007766B1 (pt) | 2009-10-08 | 2021-11-09 | Universite Libre De Bruxelles | Microscópio holográfico digital fora do eixo e método para registrar hologramas digitais fora do eixo |
EP3671176B1 (fr) | 2009-10-20 | 2022-04-13 | The Regents of the University of California | Cellule holographique incohérente sans lentille et microscopie sur une puce |
JP5887275B2 (ja) | 2009-12-04 | 2016-03-16 | ライフ テクノロジーズ コーポレーション | アコースティックフローサイトメトリーのための装置、システム、方法、およびコンピュータ読み取り可能な媒体 |
CN101727059B (zh) * | 2009-12-22 | 2011-11-30 | 暨南大学 | 基于表面等离子体共振的数字全息显微成像方法及显微镜 |
US8937756B2 (en) | 2010-02-09 | 2015-01-20 | Phase Holographic Imaging Phi Ab | Method for and use of digital holographic microscopy and imaging on labelled cell samples |
DE102010023099B3 (de) | 2010-06-09 | 2011-11-17 | Celltool Gmbh | Verfahren und Vorrichtung zum Charakterisieren von biologischen Objekten |
US9569664B2 (en) * | 2010-10-26 | 2017-02-14 | California Institute Of Technology | Methods for rapid distinction between debris and growing cells |
US9085643B2 (en) * | 2010-11-24 | 2015-07-21 | Hamlet Pharma Ab | Biologically active complex and its preparation |
EP2683831B1 (fr) | 2011-03-07 | 2015-09-23 | Accelerate Diagnostics, Inc. | Systèmes de purification rapide de cellules |
CN102122063A (zh) * | 2011-03-09 | 2011-07-13 | 北京工业大学 | 倒置式数字全息显微镜 |
BR112014001238A2 (pt) * | 2011-07-19 | 2017-06-13 | Ovizio Imaging Systems N V | método para a detecção de células cancerosas e/ou classificação de células numa amostra de células de líquido; sistema para a detecção de células e/ou a classificação de células cancerosas numa amostra de células; método de atualização e/ou melhoria de uma base de dados que compreende os limiares ligados á informação holográfica; e base de dados de objetos |
US20140195568A1 (en) * | 2011-07-19 | 2014-07-10 | Ovizio Imaging Systems NV/SA | Object database and object database improving method |
US20140347672A1 (en) * | 2011-07-29 | 2014-11-27 | Ecole Polytechnique Federale De Lausanne (Epfl) | Apparatus and method for quantitive phase tomography through linear scanning with coherent and non-coherent detection |
EP2626686A1 (fr) | 2012-02-13 | 2013-08-14 | Ovizio Imaging Systems NV/SA | Cytomètre de flux avec microscope holographique numérique |
CN202808799U (zh) | 2012-09-14 | 2013-03-20 | 江苏嘉语生物医药技术有限公司 | 一种可控氧环境的微生物培养检测装置 |
EP2898310B1 (fr) | 2012-09-20 | 2019-05-01 | Ovizio Imaging Systems NV/SA | Microscope holographique numérique ayant des systèmes de fluide |
EP3027723A1 (fr) | 2013-07-31 | 2016-06-08 | Ovizio Imaging Systems NV/SA | Couvercle amélioré pour surveiller des objets en suspension |
DE102014200911A1 (de) * | 2013-10-09 | 2015-04-09 | Siemens Aktiengesellschaft | In-Vitro-Verfahren zum markierungsfreien Bestimmen eines Zelltyps einer Zelle |
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- 2012-07-16 JP JP2014520634A patent/JP6092206B2/ja active Active
- 2012-07-16 KR KR1020167022933A patent/KR20160103175A/ko not_active Application Discontinuation
- 2012-07-16 EP EP12742828.2A patent/EP2734955A1/fr not_active Ceased
- 2012-07-16 CN CN201810049277.1A patent/CN108107197B/zh not_active Expired - Fee Related
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CN108107197A (zh) | 2018-06-01 |
US9684281B2 (en) | 2017-06-20 |
EP2734955A1 (fr) | 2014-05-28 |
KR20140049562A (ko) | 2014-04-25 |
KR20160103175A (ko) | 2016-08-31 |
WO2013011001A1 (fr) | 2013-01-24 |
CN103827889A (zh) | 2014-05-28 |
US20140139625A1 (en) | 2014-05-22 |
CN108107197B (zh) | 2020-05-08 |
JP6092206B2 (ja) | 2017-03-08 |
US10025271B2 (en) | 2018-07-17 |
JP2014527622A (ja) | 2014-10-16 |
CA2842377A1 (fr) | 2013-01-24 |
US20170261930A1 (en) | 2017-09-14 |
BR112014001238A2 (pt) | 2017-06-13 |
CN103827889B (zh) | 2018-01-02 |
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