CA2842248C - Glucoamylase variants and polynucleotides encoding same - Google Patents
Glucoamylase variants and polynucleotides encoding same Download PDFInfo
- Publication number
- CA2842248C CA2842248C CA2842248A CA2842248A CA2842248C CA 2842248 C CA2842248 C CA 2842248C CA 2842248 A CA2842248 A CA 2842248A CA 2842248 A CA2842248 A CA 2842248A CA 2842248 C CA2842248 C CA 2842248C
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- CA
- Canada
- Prior art keywords
- glucoamylase
- seq
- alpha
- amylase
- variant
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- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161531189P | 2011-09-06 | 2011-09-06 | |
| US61/531,189 | 2011-09-06 | ||
| US201161566046P | 2011-12-02 | 2011-12-02 | |
| US61/566,046 | 2011-12-02 | ||
| PCT/US2012/053779 WO2013036526A1 (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2842248A1 CA2842248A1 (en) | 2013-03-14 |
| CA2842248C true CA2842248C (en) | 2021-02-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CA2842248A Active CA2842248C (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9534239B2 (forum.php) |
| EP (1) | EP2748315B1 (forum.php) |
| CN (1) | CN103930543B (forum.php) |
| CA (1) | CA2842248C (forum.php) |
| DK (1) | DK2748315T3 (forum.php) |
| EA (1) | EA201490568A1 (forum.php) |
| ES (1) | ES2656556T3 (forum.php) |
| IN (1) | IN2014CN02469A (forum.php) |
| MX (1) | MX351154B (forum.php) |
| WO (1) | WO2013036526A1 (forum.php) |
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| CA2915336C (en) | 2013-07-17 | 2024-03-12 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| EP3712274A1 (en) | 2013-09-11 | 2020-09-23 | Novozymes A/S | Processes for producing fermentation products |
| CN105960457B (zh) | 2014-01-22 | 2021-09-03 | 诺维信公司 | 普鲁兰酶变体以及编码它们的多核苷酸 |
| RU2706297C2 (ru) | 2014-02-07 | 2019-11-15 | Новозимс А/С | Композиции для получения глюкозных сиропов |
| WO2015200659A1 (en) | 2014-06-25 | 2015-12-30 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
| CN106795505A (zh) * | 2014-10-23 | 2017-05-31 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| MX2017007102A (es) | 2014-12-01 | 2017-08-24 | Novozymes As | Produccion mejorada de jarabes de glucosa. |
| WO2016205127A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| CN108699573A (zh) | 2015-12-22 | 2018-10-23 | 诺维信公司 | 用于生产发酵产物的方法 |
| WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
| EP3592849A1 (en) * | 2017-03-07 | 2020-01-15 | Danisco US Inc. | Thermostable glucoamylase and methods of use, thereof |
| MX2019014142A (es) | 2017-06-02 | 2020-02-07 | Novozymes As | Levadura mejorada para produccion de etanol. |
| CA3065246A1 (en) | 2017-06-28 | 2019-01-03 | Novozymes A/S | Polypeptides having trehalase activity and polynucleotides encoding same |
| US11220679B2 (en) | 2017-08-08 | 2022-01-11 | Novozymes A/S | Polypeptides having trehalase activity |
| EP3682014A1 (en) | 2017-09-15 | 2020-07-22 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
| EP3701037A1 (en) | 2017-10-23 | 2020-09-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
| CA3089135A1 (en) | 2018-01-29 | 2019-08-01 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
| AU2019222480B9 (en) | 2018-02-15 | 2024-11-28 | Microbiogen Pty. Ltd. | Improved yeast for ethanol production |
| MX2020012754A (es) | 2018-05-31 | 2021-02-26 | Novozymes As | Procesos para mejorar el crecimiento y la productividad de la levadura. |
| US20210269833A1 (en) | 2018-07-11 | 2021-09-02 | Novozymes A/S | Processes for producing fermentation products |
| WO2020023411A1 (en) | 2018-07-25 | 2020-01-30 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| AU2019359187A1 (en) | 2018-10-08 | 2021-04-29 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| EP3918060A1 (en) | 2019-01-31 | 2021-12-08 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
| AU2020321930A1 (en) | 2019-07-26 | 2022-01-06 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| CN114423296A (zh) | 2019-08-05 | 2022-04-29 | 诺维信公司 | 从全酒糟副产物生产高蛋白饲料成分的酶共混物和工艺 |
| EP4010469A1 (en) | 2019-08-06 | 2022-06-15 | Novozymes A/S | Fusion proteins for improved enzyme expression |
| EP4073089A1 (en) | 2019-12-10 | 2022-10-19 | Novozymes A/S | Microorganism for improved pentose fermentation |
| CA3158832A1 (en) | 2019-12-16 | 2021-06-24 | Chee-Leong Soong | Processes for producing fermentation products |
| WO2022261003A1 (en) | 2021-06-07 | 2022-12-15 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| CN120380139A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有乙酰木聚糖酯酶活性的碳水化合物酯酶家族3（ce3）多肽和编码其的多核苷酸 |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| WO2024258820A2 (en) | 2023-06-13 | 2024-12-19 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| WO1989001969A1 (en) | 1987-09-04 | 1989-03-09 | Novo-Nordisk A/S | Process for the production of protein products in aspergillus and promoters for use in aspergillus |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DK81293D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| JP3649338B2 (ja) | 1994-06-03 | 2005-05-18 | ノボザイムス バイオテック，インコーポレイティド | 精製されたマイセリオフトラ・ラッカーゼ及びそれをコードする核酸 |
| CN1151762A (zh) | 1994-06-30 | 1997-06-11 | 诺沃诺尔迪斯克生物技术有限公司 | 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子 |
| BR9607013B1 (pt) | 1995-02-03 | 2011-03-09 | processo de construção de uma variante de uma alfa-amilase de bacillus original derivada de uma cepa de b. licheniformis, de b. amyloliquefaciens, de b. stearothermophilus ou de um bacillus sp. alcalofìlico. | |
| US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| DK0904360T3 (en) | 1996-04-30 | 2013-10-14 | Novozymes As | Alpha-amylasemutanter |
| EP2302027B1 (en) | 1997-10-13 | 2013-08-28 | Novozymes A/S | Alpha-amylase mutants |
| EP2386629A1 (en) | 1997-10-14 | 2011-11-16 | Darwin Molecular Corporation | Thymidine kinase mutants and fusion proteins having thymidine kinase and guanylate kinase activities |
| AR014402A1 (es) | 1997-11-26 | 2001-02-28 | Novozymes As | Glucoamilasa termoestable proceso para convertir almidon o almidon parcialmente hidrolizado en un jarabe que contiene dextrosa |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| RU2258739C2 (ru) | 1998-02-27 | 2005-08-20 | Новозимс А/С | ВАРИАНТ МАЛЬТОГЕННОЙ α-АМИЛАЗЫ |
| DK1124949T3 (da) | 1998-10-26 | 2006-11-06 | Novozymes As | Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller |
| DK2278016T3 (da) | 1999-03-22 | 2012-11-26 | Novozymes Inc | Promotorsekvenser fra Fusarium Venenatum og anvendelse deraf |
| EP1818396B1 (en) | 1999-03-30 | 2014-06-18 | Novozymes A/S | Alpha-amylase variants |
| US7884066B2 (en) * | 1999-10-05 | 2011-02-08 | The Regents Of The University Of California | NELL-1 enhanced bone mineralization |
| JP4855632B2 (ja) | 2000-08-01 | 2012-01-18 | ノボザイムス アクティーゼルスカブ | 変更された性質を有するα−アミラーゼ突然変異体 |
| ATE375388T1 (de) | 2001-07-27 | 2007-10-15 | Us Gov Health & Human Serv | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
| BR0214653B1 (pt) | 2001-12-07 | 2015-01-06 | Novozymes As | Construção de ácido nucléico, vetor de expressão recombinante, célula de microorganismo hospedeiro recombinante, método para produzir um polipeptídeo, uso de pelo menos uma protease, método para melhorar o valor nutricional de uma ração animal, aditivo para ração animal, composição de ração animal, e, método para o tratamento de proteínas egetais. |
| US7785601B2 (en) * | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| DE602004024964D1 (de) | 2003-03-10 | 2010-02-25 | Novozymes As | Verfahren zur herstellung von alkohol |
| MXPA06000212A (es) | 2003-06-25 | 2006-03-21 | Novozymes As | Enzimas para procesar almidon. |
| ATE510925T1 (de) | 2003-06-25 | 2011-06-15 | Novozymes As | Stärkehydrolyseverfahren |
| ATE441705T1 (de) | 2003-10-30 | 2009-09-15 | Novozymes As | Kohlenhydratbindende module |
| JP5452869B2 (ja) | 2004-12-22 | 2014-03-26 | ノボザイムス アクティーゼルスカブ | デンプン加工法 |
| BRPI0519985B1 (pt) * | 2005-02-23 | 2018-01-30 | University Of Kentucky Research Foundation | Método para redução do nível de nornicotina ou n-nitrosonornicotina em uma planta do gênero nicotiana ou parte desta ou em um produto de tabaco, cassete de expressão e método para a produção de uma planta do gênero nicotiana |
| DE602007003225D1 (de) | 2006-06-15 | 2009-12-24 | Novozymes North America Inc | Verfahren zur herstellung eines stärkehydrolysats |
| WO2011072191A2 (en) | 2009-12-11 | 2011-06-16 | Novozymes A/S | Protease variants |
| EP2521771A1 (en) | 2010-01-04 | 2012-11-14 | Novozymes A/S | Stabilization of alpha-amylases towards calcium depletion and acidic ph |
| ES2565060T3 (es) * | 2010-04-14 | 2016-03-31 | Novozymes A/S | Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos |
| ES2673940T3 (es) | 2010-12-22 | 2018-06-26 | Novozymes North America, Inc. | Proceso para producir productos de fermentación a partir de materiales que contienen almidón |
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Also Published As
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|---|---|
| DK2748315T3 (en) | 2018-02-05 |
| EA201490568A1 (ru) | 2014-06-30 |
| EP2748315B1 (en) | 2017-11-15 |
| CA2842248A1 (en) | 2013-03-14 |
| ES2656556T3 (es) | 2018-02-27 |
| WO2013036526A1 (en) | 2013-03-14 |
| MX2014002415A (es) | 2014-05-28 |
| US9534239B2 (en) | 2017-01-03 |
| CN103930543A (zh) | 2014-07-16 |
| EP2748315A1 (en) | 2014-07-02 |
| MX351154B (es) | 2017-10-04 |
| IN2014CN02469A (forum.php) | 2015-06-19 |
| US20140342422A1 (en) | 2014-11-20 |
| CN103930543B (zh) | 2020-11-27 |
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