CA2811888A1 - Integrated cartridge - Google Patents
Integrated cartridge Download PDFInfo
- Publication number
- CA2811888A1 CA2811888A1 CA2811888A CA2811888A CA2811888A1 CA 2811888 A1 CA2811888 A1 CA 2811888A1 CA 2811888 A CA2811888 A CA 2811888A CA 2811888 A CA2811888 A CA 2811888A CA 2811888 A1 CA2811888 A1 CA 2811888A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- chamber
- cartridge
- integrated cartridge
- integrated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000002493 microarray Methods 0.000 claims abstract description 67
- 238000000746 purification Methods 0.000 claims abstract description 63
- 238000002360 preparation method Methods 0.000 claims abstract description 56
- 238000004458 analytical method Methods 0.000 claims abstract description 49
- 238000000605 extraction Methods 0.000 claims abstract description 32
- 239000012530 fluid Substances 0.000 claims abstract description 32
- 238000012545 processing Methods 0.000 claims abstract description 20
- 238000004891 communication Methods 0.000 claims abstract description 17
- 230000003287 optical effect Effects 0.000 claims description 52
- 239000011324 bead Substances 0.000 claims description 31
- 239000002699 waste material Substances 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 27
- 230000006037 cell lysis Effects 0.000 claims description 26
- 238000010828 elution Methods 0.000 claims description 25
- 238000005286 illumination Methods 0.000 claims description 23
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 238000003384 imaging method Methods 0.000 claims description 20
- 108090000623 proteins and genes Proteins 0.000 claims description 20
- 230000008569 process Effects 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 230000003321 amplification Effects 0.000 claims description 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 150000007523 nucleic acids Chemical group 0.000 claims description 15
- 238000003760 magnetic stirring Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 8
- 230000033001 locomotion Effects 0.000 claims description 7
- 238000000799 fluorescence microscopy Methods 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000000523 sample Substances 0.000 description 168
- 238000003752 polymerase chain reaction Methods 0.000 description 49
- 210000004027 cell Anatomy 0.000 description 37
- 238000013022 venting Methods 0.000 description 25
- 239000012298 atmosphere Substances 0.000 description 22
- 108020004414 DNA Proteins 0.000 description 21
- -1 poly(vinylpyrrolidone) Polymers 0.000 description 16
- 239000000872 buffer Substances 0.000 description 15
- 239000000203 mixture Substances 0.000 description 15
- 238000010438 heat treatment Methods 0.000 description 13
- 238000003756 stirring Methods 0.000 description 13
- 239000006285 cell suspension Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 239000000835 fiber Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 238000001742 protein purification Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 239000012491 analyte Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 239000011534 wash buffer Substances 0.000 description 9
- 238000003491 array Methods 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 238000001821 nucleic acid purification Methods 0.000 description 8
- 241000193996 Streptococcus pyogenes Species 0.000 description 7
- 230000009089 cytolysis Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 108700028369 Alleles Proteins 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 108091093088 Amplicon Proteins 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000009396 hybridization Methods 0.000 description 5
- 229920003023 plastic Polymers 0.000 description 5
- 239000004033 plastic Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 239000000654 additive Substances 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 238000003032 molecular docking Methods 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000003260 vortexing Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000193738 Bacillus anthracis Species 0.000 description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000002457 bidirectional effect Effects 0.000 description 3
- 239000012149 elution buffer Substances 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 210000001236 prokaryotic cell Anatomy 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 101100458361 Drosophila melanogaster SmydA-8 gene Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000005441 aurora Substances 0.000 description 2
- 229940065181 bacillus anthracis Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229960000686 benzalkonium chloride Drugs 0.000 description 2
- 229960001950 benzethonium chloride Drugs 0.000 description 2
- UREZNYTWGJKWBI-UHFFFAOYSA-M benzethonium chloride Chemical compound [Cl-].C1=CC(C(C)(C)CC(C)(C)C)=CC=C1OCCOCC[N+](C)(C)CC1=CC=CC=C1 UREZNYTWGJKWBI-UHFFFAOYSA-M 0.000 description 2
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical compound [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 2
- 229960001927 cetylpyridinium chloride Drugs 0.000 description 2
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- SMVRDGHCVNAOIN-UHFFFAOYSA-L disodium;1-dodecoxydodecane;sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCCOCCCCCCCCCCCC SMVRDGHCVNAOIN-UHFFFAOYSA-L 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 238000007403 mPCR Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229920001451 polypropylene glycol Polymers 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000003134 recirculating effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical class OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- QZTKDVCDBIDYMD-UHFFFAOYSA-N 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid Chemical compound NC(=O)CN(CC(O)=O)CC(O)=O QZTKDVCDBIDYMD-UHFFFAOYSA-N 0.000 description 1
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 description 1
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 description 1
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 1
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 description 1
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 description 1
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 description 1
- VTOWJTPBPWTSMK-UHFFFAOYSA-N 4-morpholin-4-ylbutane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCCN1CCOCC1 VTOWJTPBPWTSMK-UHFFFAOYSA-N 0.000 description 1
- 239000007991 ACES buffer Substances 0.000 description 1
- 239000007988 ADA buffer Substances 0.000 description 1
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 description 1
- FOXXZZGDIAQPQI-XKNYDFJKSA-N Asp-Pro-Ser-Ser Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O FOXXZZGDIAQPQI-XKNYDFJKSA-N 0.000 description 1
- 239000007992 BES buffer Substances 0.000 description 1
- 239000007989 BIS-Tris Propane buffer Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000001543 Corylus americana Nutrition 0.000 description 1
- 240000007582 Corylus avellana Species 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102100034745 E3 ubiquitin-protein ligase HERC2 Human genes 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 description 1
- 239000007996 HEPPS buffer Substances 0.000 description 1
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 description 1
- 101000872516 Homo sapiens E3 ubiquitin-protein ligase HERC2 Proteins 0.000 description 1
- 101001011441 Homo sapiens Interferon regulatory factor 4 Proteins 0.000 description 1
- 102100030126 Interferon regulatory factor 4 Human genes 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 102100037258 Membrane-associated transporter protein Human genes 0.000 description 1
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 description 1
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 description 1
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 description 1
- 102100034574 P protein Human genes 0.000 description 1
- 239000007990 PIPES buffer Substances 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 229920002732 Polyanhydride Polymers 0.000 description 1
- 108091006702 SLC24A4 Proteins 0.000 description 1
- 108091007563 SLC45A2 Proteins 0.000 description 1
- 102100032003 Sodium/potassium/calcium exchanger 4 Human genes 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 206010041925 Staphylococcal infections Diseases 0.000 description 1
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 description 1
- 239000007994 TES buffer Substances 0.000 description 1
- 239000007997 Tricine buffer Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 150000004996 alkyl benzenes Chemical class 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 1
- 229940063953 ammonium lauryl sulfate Drugs 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 229940077388 benzenesulfonate Drugs 0.000 description 1
- 239000007998 bicine buffer Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- HHKZCCWKTZRCCL-UHFFFAOYSA-N bis-tris propane Chemical compound OCC(CO)(CO)NCCCNC(CO)(CO)CO HHKZCCWKTZRCCL-UHFFFAOYSA-N 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000008004 cell lysis buffer Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 description 1
- 229940073507 cocamidopropyl betaine Drugs 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 229910052809 inorganic oxide Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910001507 metal halide Inorganic materials 0.000 description 1
- 150000005309 metal halides Chemical class 0.000 description 1
- 208000015688 methicillin-resistant staphylococcus aureus infectious disease Diseases 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000010363 phase shift Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000009428 plumbing Methods 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001987 poloxamine Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 229920001289 polyvinyl ether Polymers 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- ASHGTUMKRVIOLH-UHFFFAOYSA-L potassium;sodium;hydrogen phosphate Chemical compound [Na+].[K+].OP([O-])([O-])=O ASHGTUMKRVIOLH-UHFFFAOYSA-L 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 102220001747 rs16891982 Human genes 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000003746 surface roughness Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Micromachines (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27239709P | 2009-09-21 | 2009-09-21 | |
| US61/272,397 | 2009-09-21 | ||
| PCT/US2010/002568 WO2011034620A2 (en) | 2009-09-21 | 2010-09-20 | Integrated cartridge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2811888A1 true CA2811888A1 (en) | 2011-03-24 |
Family
ID=43757137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2811888A Abandoned CA2811888A1 (en) | 2009-09-21 | 2010-09-20 | Integrated cartridge |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US8623789B2 (enExample) |
| EP (1) | EP2480896A4 (enExample) |
| JP (4) | JP5671540B2 (enExample) |
| CN (2) | CN105675496B (enExample) |
| CA (1) | CA2811888A1 (enExample) |
| IN (1) | IN2012DN03430A (enExample) |
| WO (1) | WO2011034620A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108499410A (zh) * | 2018-04-28 | 2018-09-07 | 王书华 | 一种可搅拌试剂盒 |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10125388B2 (en) | 2007-10-31 | 2018-11-13 | Akonni Biosystems, Inc. | Integrated sample processing system |
| US9791386B2 (en) * | 2009-01-20 | 2017-10-17 | Spectro Scientific, Inc. | Integrated, portable sample analysis system and method |
| ITTO20100068U1 (it) * | 2010-04-20 | 2011-10-21 | Eltek Spa | Dispositivi microfluidici e/o attrezzature per dispositivi microfluidici |
| CN103403545B (zh) * | 2010-12-09 | 2018-06-22 | 阿科尼生物系统公司 | 样本分析系统 |
| WO2012142397A2 (en) | 2011-04-13 | 2012-10-18 | Akonni Biosystems, Inc. | Microarray based sample detection system |
| JP5892589B2 (ja) * | 2011-11-24 | 2016-03-23 | マイクロ化学技研株式会社 | マイクロデバイス及びバイオアッセイシステム |
| WO2013086486A1 (en) | 2011-12-09 | 2013-06-13 | President And Fellows Of Harvard College | Integrated human organ-on-chip microphysiological systems |
| CA3191015A1 (en) | 2012-02-07 | 2013-08-15 | Vibrant Holdings, Llc | Substrates, peptide arrays, and methods |
| NL2008737C2 (nl) * | 2012-05-01 | 2013-11-04 | Consultatie Implementatie Tech Beheer B V | Afsluitelement voor het afsluiten van een houder voor monsters. |
| KR102041217B1 (ko) * | 2012-05-30 | 2019-11-07 | 주식회사 미코바이오메드 | 다-채널 하향 액체 주입 장치, 이를 포함하는 핵산 추출 장치, 및 이를 이용한 핵산 추출 방법 |
| US10139322B2 (en) | 2012-07-03 | 2018-11-27 | Merck Patent Gmbh | Sample preparation device |
| EP2901159B1 (en) * | 2012-09-28 | 2020-02-26 | Vibrant Holdings, LLC | Methods, systems, and arrays for biomolecular analysis |
| US10006909B2 (en) | 2012-09-28 | 2018-06-26 | Vibrant Holdings, Llc | Methods, systems, and arrays for biomolecular analysis |
| DE102012109317A1 (de) * | 2012-10-01 | 2014-04-03 | Astrium Gmbh | Vorrichtung zur Durchführung einer biochemischen Analyse, insbesondere im Weltraum |
| US10286376B2 (en) | 2012-11-14 | 2019-05-14 | Vibrant Holdings, Llc | Substrates, systems, and methods for array synthesis and biomolecular analysis |
| EP2956771B1 (en) | 2013-02-15 | 2020-04-08 | Vibrant Holdings, LLC | Methods and compositions for amplified electrochemiluminescence detection |
| US10058866B2 (en) | 2013-03-13 | 2018-08-28 | Abbott Laboratories | Methods and apparatus to mitigate bubble formation in a liquid |
| US9535082B2 (en) | 2013-03-13 | 2017-01-03 | Abbott Laboratories | Methods and apparatus to agitate a liquid |
| USD978375S1 (en) | 2013-03-13 | 2023-02-14 | Abbott Laboratories | Reagent container |
| USD962471S1 (en) | 2013-03-13 | 2022-08-30 | Abbott Laboratories | Reagent container |
| CN107991479B (zh) | 2013-05-20 | 2021-10-22 | 硅生物装置有限公司 | 片上磁粒子检测的数字控制 |
| JP6178161B2 (ja) * | 2013-08-21 | 2017-08-09 | 株式会社日立ハイテクノロジーズ | フローセル固定装置及び核酸分析装置 |
| US9568404B2 (en) | 2014-05-16 | 2017-02-14 | Junyu Mai | Method and apparatus for biomolecule analysis |
| EP2950076B1 (en) * | 2014-05-26 | 2017-06-28 | CSEM Centre Suisse d'Electronique et de Microtechnique SA - Recherche et Développement | Portable system for automated preparation of liquid and/or solid samples |
| EP3151965B1 (en) | 2014-06-04 | 2021-02-24 | Edan Instruments, Inc. | Sample collection and analysis devices |
| WO2015195918A1 (en) | 2014-06-19 | 2015-12-23 | Akonni Biosystems, Inc. | Molecular analysis system and use thereof |
| WO2016053621A1 (en) * | 2014-09-16 | 2016-04-07 | Sri International | Affinity reagent and catalyst discovery though fiber-optic array scanning technology |
| US9885352B2 (en) | 2014-11-25 | 2018-02-06 | Genia Technologies, Inc. | Selectable valve of a delivery system |
| USD815752S1 (en) * | 2014-11-28 | 2018-04-17 | Randox Laboratories Ltd. | Biochip well |
| US11377651B2 (en) * | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| CA3222708A1 (en) | 2015-06-02 | 2016-12-08 | Biofire Defense Llc | Sample to sequence |
| EP3349899A4 (en) * | 2015-09-14 | 2019-03-27 | Singulex, Inc. | SINGLE MOLECULE DETECTION ON A CHIP |
| CN105301267A (zh) * | 2015-11-16 | 2016-02-03 | 常州大学 | 一种烟煤灼烧物自动过滤清洗的操作端转动齿传设备 |
| CN105733927A (zh) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | 一种用于核酸提取的耗材及多联耗材 |
| EP3301431B1 (en) | 2016-09-29 | 2019-08-28 | Roche Diagniostics GmbH | Multi-chamber analysis device and method for analyzing |
| JP7048586B6 (ja) * | 2016-09-30 | 2022-06-01 | コーニンクレッカ フィリップス エヌ ヴェ | サンプルを調製するシステム |
| IL290157B2 (en) * | 2016-10-14 | 2023-09-01 | Illumina Inc | cartridge kit |
| GB201704768D0 (en) * | 2017-01-05 | 2017-05-10 | Illumina Inc | Flow cell liquid degassing systema and method |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| CN110998333B (zh) * | 2017-05-16 | 2023-08-25 | Sk电信有限公司 | 使用盒的核酸分析设备 |
| WO2018218250A2 (en) | 2017-05-26 | 2018-11-29 | Vibrant Holdings, Llc | Photoactive compounds and methods for biomolecule detection and sequencing |
| CN107192714B (zh) * | 2017-06-01 | 2020-03-27 | 首钢集团有限公司 | 一种测量浸入式水口堵塞程度的方法及系统 |
| WO2019043779A1 (ja) * | 2017-08-29 | 2019-03-07 | 横河電機株式会社 | 核酸抽出方法および核酸抽出装置 |
| KR102043502B1 (ko) * | 2017-09-04 | 2019-11-11 | 한국과학기술원 | 바이옵시 장치 |
| CN107385103B (zh) * | 2017-09-18 | 2023-04-07 | 星源智(珠海)生物科技有限公司 | 一种非纯化核酸扩增方法及装置 |
| CN107727425A (zh) * | 2017-10-25 | 2018-02-23 | 哈尔滨师范大学 | 一种小型哺乳动物代谢物收集分析器 |
| US11110457B2 (en) | 2017-12-28 | 2021-09-07 | Stmicroelectronics S.R.L. | Analysis unit for a transportable microfluidic device, in particular for sample preparation and molecule analysis |
| US11278897B2 (en) | 2017-12-28 | 2022-03-22 | Stmicroelectronics S.R.L. | Cartridge for sample preparation and molecule analysis, cartridge control machine, sample preparation system and method using the cartridge |
| US11511278B2 (en) | 2017-12-28 | 2022-11-29 | Stmicroelectronics S.R.L. | Solid reagent containment unit, in particular for a portable microfluidic device for sample preparation and molecule analysis |
| US11491489B2 (en) * | 2017-12-28 | 2022-11-08 | Stmicroelectronics S.R.L. | Microfluidic connector group, microfluidic device and manufacturing process thereof, in particular for a cartridge for sample preparation and molecule analysis |
| US11717825B2 (en) | 2017-12-28 | 2023-08-08 | Stmicroelectronics S.R.L. | Magnetically controllable valve and portable microfluidic device having a magnetically controllable valve, in particular cartridge for sample preparation and molecule analysis |
| JP7422087B2 (ja) | 2018-05-09 | 2024-01-25 | ヴィブラント ホールディングス リミテッド ライアビリティ カンパニー | 光活性化された作用物質を使用してポリヌクレオチドアレイを合成する方法 |
| CN108796038B (zh) * | 2018-06-26 | 2019-10-18 | 杭州优思达生物技术有限公司 | 一种核酸一体化检测方法及检测试剂管 |
| CN109112060B (zh) * | 2018-09-13 | 2021-09-14 | 青岛大学附属医院 | 一种微生物样本涂片笔 |
| AU2019388865B2 (en) | 2018-11-30 | 2025-05-29 | Illumina Singapore Pte. Ltd. | Temperature-controllable reagent cartridge and temperature control system for the same |
| CN109647552B (zh) * | 2018-12-27 | 2021-09-28 | 南京祥中生物科技有限公司 | 集成式的微阵列生物芯片及识别方法 |
| US12233415B2 (en) | 2019-04-29 | 2025-02-25 | Hewlett-Packard Development Company, L.P. | Microfluidic reaction chamber for amplification of nucleic acids |
| US20220362767A1 (en) * | 2019-10-25 | 2022-11-17 | Fabrico Technology, Inc. | Systems and methods for measuring colorimetric reactions |
| US11060138B1 (en) * | 2020-01-17 | 2021-07-13 | Element Biosciences, Inc. | Nucleic acid sequencing systems |
| JP7290345B2 (ja) * | 2020-02-25 | 2023-06-13 | Blue Industries株式会社 | 遺伝子解析用前処理キット、核酸分析用チップ、解析システム、生体物質分析用チップ |
| CN113528317A (zh) * | 2020-04-15 | 2021-10-22 | 拓原合壹(宁波)生物技术有限公司 | 核酸提取方法 |
| GB2594980B (en) * | 2020-05-14 | 2025-01-01 | Agilent Tech Lda Uk Limited | Spectral analysis of a sample |
| GB202009823D0 (en) * | 2020-06-26 | 2020-08-12 | Stimson William Howard | Automated quantitative assay device and a method of performing the quantitative assays |
| KR102569853B1 (ko) * | 2020-11-23 | 2023-08-24 | (주)마이크로디지탈 | 반응웰 카트리지 장치 및 반응웰 카트리지 시스템 |
| US20220162585A1 (en) * | 2020-11-23 | 2022-05-26 | Frank Leo Spangler | System and method of nucleic acid extraction |
| US11806713B2 (en) * | 2020-11-30 | 2023-11-07 | Nexogen, Inc. | Microfluidic system based on active control of flow resistance in microfluidic channels and methods of use thereof |
| CN113416629B (zh) * | 2021-06-03 | 2023-09-22 | 中南大学湘雅三医院 | 一种肿瘤化疗药物敏感性检测试剂盒和检测方法 |
| EP4363112A1 (en) * | 2021-07-01 | 2024-05-08 | Cepheid | Universal assay cartridge and methods of use |
| IL310281A (en) | 2021-07-21 | 2024-03-01 | Element Biosciences Inc | Optical systems for nucleic acid sequencing and methods thereof |
| TWI815219B (zh) * | 2021-11-19 | 2023-09-11 | 台達電子工業股份有限公司 | 核酸處理裝置及其樣本前處理模組 |
| KR102814100B1 (ko) * | 2022-08-02 | 2025-05-28 | 주식회사 아이젠텍 | 질병의 진단을 위한 올인원 카트리지 및 진단기기 |
| CN118425050B (zh) * | 2024-04-25 | 2024-12-17 | 北京师范大学 | 多反气室 |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5230866A (en) * | 1991-03-01 | 1993-07-27 | Biotrack, Inc. | Capillary stop-flow junction having improved stability against accidental fluid flow |
| US5305139A (en) * | 1991-04-19 | 1994-04-19 | Unimat (Usa) Corporation, Formerly United Holdings, Inc. | Illumination system and method for a high definition 3-D light microscope |
| US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
| JP3326881B2 (ja) * | 1993-06-25 | 2002-09-24 | 株式会社ニコン | 走査型光学顕微鏡 |
| RU2041261C1 (ru) | 1993-08-11 | 1995-08-09 | Институт молекулярной биологии им.В.А.Энгельгардта РАН | Способ изготовления матрицы для детектирования мисматчей |
| RU2041262C1 (ru) | 1993-08-11 | 1995-08-09 | Институт молекулярной биологии им.В.А.Энгельгардта РАН | Способ иммобилизации водорастворимых биоорганических соединений на капиллярно-пористый носитель |
| WO1999001221A1 (de) * | 1997-06-30 | 1999-01-14 | INSTITUT FüR MIKROTECHNIK MAINZ GMBH | Mikroreaktionsgefäss, anordnung von mikroreaktionsgefässen sowie verfahren zur abgabe einer flüssigkeit aus den selben |
| US5981734A (en) | 1997-07-17 | 1999-11-09 | University Of Chicago | Methods for immobilizing nucleic acids on a gel substrate |
| JP4503716B2 (ja) * | 1997-08-29 | 2010-07-14 | オリンパス株式会社 | 顕微鏡透過照明装置 |
| US6656725B2 (en) | 2001-08-15 | 2003-12-02 | The University Of Chicago | Method of fabrication of microarray of gel-immobilized compounds on a chip by copolymerization |
| AU2003237340A1 (en) * | 2002-05-28 | 2003-12-12 | Autogenomics, Inc. | Level-controlled pipette for automated analytic devices |
| US7148057B2 (en) * | 2002-08-13 | 2006-12-12 | Gongin Precision Industries, Co., Ltd | Array biochip workstation |
| EP2315027A1 (en) * | 2002-08-16 | 2011-04-27 | Decision Biomarkers Incorporated | Substrates for isolating, reacting and microscopically analyzing materials |
| JP2004078690A (ja) * | 2002-08-20 | 2004-03-11 | Olympus Corp | 画像比較装置、画像比較方法および画像比較をコンピュータにて実行させるためのプログラム |
| US20060094108A1 (en) * | 2002-12-20 | 2006-05-04 | Karl Yoder | Thermal cycler for microfluidic array assays |
| JP4198982B2 (ja) * | 2002-12-26 | 2008-12-17 | オリンパス株式会社 | 波長分離素子および顕微鏡 |
| WO2004063731A1 (ja) * | 2003-01-16 | 2004-07-29 | Olympus Corporation | 光検出装置 |
| DE10315074A1 (de) * | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren |
| JP2004309426A (ja) * | 2003-04-10 | 2004-11-04 | Denso Corp | 透光性基板の評価方法及び透光性基板の評価装置 |
| JP4354207B2 (ja) * | 2003-04-15 | 2009-10-28 | オリンパス株式会社 | 顕微鏡装置 |
| US20050042146A1 (en) * | 2003-08-19 | 2005-02-24 | Fuji Photo Film Co., Ltd. | Rack for extracting apparatuses |
| JP4993653B2 (ja) * | 2003-10-03 | 2012-08-08 | キヤノン株式会社 | 記録材判別装置および画像形成装置並びにその方法 |
| WO2005079543A2 (en) * | 2004-02-19 | 2005-09-01 | Waters Investments Limited | Pin valve assembly |
| DE102005052713A1 (de) * | 2005-11-04 | 2007-05-16 | Clondiag Chip Tech Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| DE102004053730B4 (de) * | 2004-11-06 | 2014-04-03 | Carl Zeiss Jena Gmbh | Verfahren und Anordnung zur Unterdrückung von Falschlicht |
| US20070031862A1 (en) | 2005-08-08 | 2007-02-08 | The University Of Chicago | Preparation of plastic supports for biochips |
| JP4632899B2 (ja) * | 2005-08-17 | 2011-02-16 | 丸茂電機株式会社 | Ledスポットライト |
| JP4687413B2 (ja) * | 2005-11-16 | 2011-05-25 | コニカミノルタエムジー株式会社 | マイクロチップにおける2種類以上の液体の混合方法およびマイクロ総合分析システム |
| JP2007171144A (ja) * | 2005-12-26 | 2007-07-05 | Toray Ind Inc | カバーを有するマイクロアレイ |
| AU2007254191B2 (en) * | 2006-05-16 | 2013-01-10 | Fluidigm Corporation | PCR-free sample preparation and detection systems for high speed biologic analysis and identification |
| US20080153078A1 (en) * | 2006-06-15 | 2008-06-26 | Braman Jeffrey C | System for isolating biomolecules from a sample |
| JP4909672B2 (ja) * | 2006-08-08 | 2012-04-04 | 株式会社日本マイクロニクス | 液晶パネル検査方法及び装置 |
| JP2008180578A (ja) * | 2007-01-24 | 2008-08-07 | Toppan Printing Co Ltd | 周期性パターンのムラ検査装置 |
| JP4869129B2 (ja) * | 2007-03-30 | 2012-02-08 | Hoya株式会社 | パターン欠陥検査方法 |
| JP2009002899A (ja) * | 2007-06-25 | 2009-01-08 | Akita Prefecture | 検出装置 |
| US7955841B2 (en) | 2007-08-23 | 2011-06-07 | Akonni Biosystems | Temperature control device with a flexible temperature control surface |
| US7955840B2 (en) | 2007-08-23 | 2011-06-07 | Akonni Biosystems | Thermal cycler for PCR including temperature control bladder |
| JP5523327B2 (ja) * | 2007-10-12 | 2014-06-18 | レオニックス,インコーポレイテッド | 統合型マイクロ流体デバイスおよび方法 |
| WO2009054254A1 (ja) * | 2007-10-26 | 2009-04-30 | Konica Minolta Medical & Graphic, Inc. | マイクロチップとそれを用いた検査装置 |
| US7759112B2 (en) | 2007-10-31 | 2010-07-20 | Akonni Biosystems, Inc. | Apparatus, system, and method for purifying nucleic acids |
| US20090111193A1 (en) * | 2007-10-31 | 2009-04-30 | Cooney Christopher G | Sample preparation device |
| US8680025B2 (en) | 2008-05-09 | 2014-03-25 | Akonni Biosystems, Inc. | Microarray system |
| BRPI1010169A2 (pt) * | 2009-06-05 | 2016-03-29 | Integenx Inc | sistema que se ajusta dentro de um invólucro de não mais que 10 pés3, cartucho, artigo em forma legível por computador, método, sistema configurado para realizar um método, sistema óptico, instrumento e dispositivo. |
-
2010
- 2010-09-20 WO PCT/US2010/002568 patent/WO2011034620A2/en not_active Ceased
- 2010-09-20 EP EP10817589.4A patent/EP2480896A4/en not_active Withdrawn
- 2010-09-20 CA CA2811888A patent/CA2811888A1/en not_active Abandoned
- 2010-09-20 JP JP2012529753A patent/JP5671540B2/ja not_active Expired - Fee Related
- 2010-09-20 CN CN201610024070.XA patent/CN105675496B/zh not_active Expired - Fee Related
- 2010-09-20 CN CN201080051244.2A patent/CN102667489B/zh not_active Expired - Fee Related
- 2010-09-20 IN IN3430DEN2012 patent/IN2012DN03430A/en unknown
- 2010-09-20 US US12/886,201 patent/US8623789B2/en active Active
-
2012
- 2012-07-05 US US13/542,111 patent/US9074245B2/en active Active
-
2014
- 2014-12-20 JP JP2014258146A patent/JP5993928B2/ja not_active Expired - Fee Related
-
2016
- 2016-08-22 JP JP2016161714A patent/JP6370847B2/ja not_active Expired - Fee Related
-
2018
- 2018-07-11 JP JP2018131183A patent/JP2019002933A/ja active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108499410A (zh) * | 2018-04-28 | 2018-09-07 | 王书华 | 一种可搅拌试剂盒 |
| CN108499410B (zh) * | 2018-04-28 | 2020-03-17 | 浙江伊利康生物技术有限公司 | 一种可搅拌试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2017029152A (ja) | 2017-02-09 |
| JP2019002933A (ja) | 2019-01-10 |
| HK1175843A1 (zh) | 2013-07-12 |
| JP2015092175A (ja) | 2015-05-14 |
| US20110071055A1 (en) | 2011-03-24 |
| US9074245B2 (en) | 2015-07-07 |
| CN105675496B (zh) | 2019-07-05 |
| CN102667489B (zh) | 2016-02-10 |
| US20130079253A1 (en) | 2013-03-28 |
| JP6370847B2 (ja) | 2018-08-08 |
| JP5993928B2 (ja) | 2016-09-14 |
| US20120004143A2 (en) | 2012-01-05 |
| WO2011034620A3 (en) | 2011-06-03 |
| JP5671540B2 (ja) | 2015-02-18 |
| CN102667489A (zh) | 2012-09-12 |
| CN105675496A (zh) | 2016-06-15 |
| JP2013505442A (ja) | 2013-02-14 |
| IN2012DN03430A (enExample) | 2015-10-23 |
| EP2480896A2 (en) | 2012-08-01 |
| EP2480896A4 (en) | 2015-12-02 |
| US8623789B2 (en) | 2014-01-07 |
| WO2011034620A2 (en) | 2011-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9074245B2 (en) | Microarray-based sample analysis system | |
| CN112368077B (zh) | 流动池装置及其用途 | |
| CN101415813B (zh) | 微流体装置 | |
| KR101214780B1 (ko) | 미세유동 장치 | |
| EP2032722B1 (en) | Pcr-free sample preparation and detection systems for high speed biologic analysis and identification | |
| US9283563B2 (en) | Systems and methods for real-time PCR | |
| EP1608952B1 (en) | Assay apparatus and method using microfluidic arrays | |
| WO2015160863A1 (en) | Portable nucleic acid analysis systemand high-performance microfluidic electroactive polymer actuators | |
| AU2005240757A1 (en) | Device and method for detecting molecular interactions | |
| HK1225104A1 (en) | Integrated cartridge | |
| HK1175843B (en) | Integrated cartridge | |
| HK1225104B (zh) | 一体化料筒 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20150817 |
|
| FZDE | Discontinued |
Effective date: 20210104 |