CA2803940C - Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping - Google Patents
Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping Download PDFInfo
- Publication number
- CA2803940C CA2803940C CA2803940A CA2803940A CA2803940C CA 2803940 C CA2803940 C CA 2803940C CA 2803940 A CA2803940 A CA 2803940A CA 2803940 A CA2803940 A CA 2803940A CA 2803940 C CA2803940 C CA 2803940C
- Authority
- CA
- Canada
- Prior art keywords
- hla
- pcr
- dqb1
- dna
- sequencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010213721 CN101921842B (zh) | 2010-06-30 | 2010-06-30 | Hla-a,b基因分型用pcr引物及其使用方法 |
| CN201010213719.5A CN101921841B (zh) | 2010-06-30 | 2010-06-30 | 基于Illumina GA测序技术的HLA基因高分辨率分型方法 |
| CN201010213721.2 | 2010-06-30 | ||
| CN201010213717.6 | 2010-06-30 | ||
| CN201010213717.6A CN101921840B (zh) | 2010-06-30 | 2010-06-30 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
| CN201010213719.5 | 2010-06-30 | ||
| CNPCT/CN2010/002150 | 2010-12-24 | ||
| CNPCT/CN2010/002149 | 2010-12-24 | ||
| PCT/CN2010/002150 WO2012083506A1 (zh) | 2010-12-24 | 2010-12-24 | Hla-dqb1基因分型的方法及其相关引物 |
| PCT/CN2010/002149 WO2012083505A1 (zh) | 2010-12-24 | 2010-12-24 | Hla-c基因分型的方法及其相关引物 |
| PCT/CN2011/076688 WO2012000445A1 (zh) | 2010-06-30 | 2011-06-30 | 新的的pcr测序方法及其在hla基因分型中的应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2803940A1 CA2803940A1 (en) | 2012-01-05 |
| CA2803940C true CA2803940C (en) | 2019-07-02 |
Family
ID=45401405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2803940A Active CA2803940C (en) | 2010-06-30 | 2011-06-30 | Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9957564B2 (OSRAM) |
| EP (1) | EP2599877B1 (OSRAM) |
| JP (1) | JP5968879B2 (OSRAM) |
| KR (1) | KR101709826B1 (OSRAM) |
| AU (1) | AU2011274090B2 (OSRAM) |
| BR (1) | BR112012032586B1 (OSRAM) |
| CA (1) | CA2803940C (OSRAM) |
| DK (1) | DK2599877T3 (OSRAM) |
| MY (1) | MY173793A (OSRAM) |
| RU (1) | RU2587606C2 (OSRAM) |
| SG (1) | SG186876A1 (OSRAM) |
| WO (1) | WO2012000445A1 (OSRAM) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11859171B2 (en) | 2013-04-17 | 2024-01-02 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| JP6308724B2 (ja) | 2013-05-09 | 2018-04-11 | ジェノダイブファーマ株式会社 | Ｈｌａ遺伝子のマルチプレックスｄｎａタイピング方法及びキット |
| JP2016528913A (ja) * | 2013-08-26 | 2016-09-23 | ザ・トランスレーショナル・ジェノミクス・リサーチ・インスティチュート | 病原体試料におけるマイナー変異体突然変異検出のための単一分子重複読み取り分析 |
| GB201409282D0 (en) | 2014-05-23 | 2014-07-09 | Univ Sydney Tech | Sequencing process |
| JP2017525369A (ja) * | 2014-09-05 | 2017-09-07 | キアゲン ゲーエムベーハー | アダプター連結アンプリコンの調製 |
| WO2016037358A1 (zh) * | 2014-09-12 | 2016-03-17 | 深圳华大基因科技有限公司 | 分离的寡核苷酸及其在核酸测序中的用途 |
| CN105400864B (zh) * | 2014-09-12 | 2020-04-14 | 深圳华大基因股份有限公司 | 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途 |
| US10233490B2 (en) | 2014-11-21 | 2019-03-19 | Metabiotech Corporation | Methods for assembling and reading nucleic acid sequences from mixed populations |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| WO2016100049A1 (en) | 2014-12-18 | 2016-06-23 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
| WO2016124069A1 (zh) * | 2015-02-04 | 2016-08-11 | 深圳华大基因研究院 | 一种构建长片段测序文库的方法 |
| KR101651817B1 (ko) | 2015-10-28 | 2016-08-29 | 대한민국 | Ngs 라이브러리 제작용 프라이머 세트 및 이를 이용한 ngs 라이브러리 제작방법 및 키트 |
| CN108699600A (zh) * | 2016-02-23 | 2018-10-23 | 诺维信公司 | 改进的新一代测序 |
| EP3459115A4 (en) | 2016-05-16 | 2020-04-08 | Agilome, Inc. | GRAPHENE FETING DEVICES, SYSTEMS, AND METHODS OF USE THEREOF FOR NUCLEIC ACID SEQUENCING |
| CN110312825A (zh) | 2016-10-24 | 2019-10-08 | 吉内恩福赛克公司 | 隐藏核酸内存在的信息 |
| US11441192B2 (en) | 2016-11-15 | 2022-09-13 | Quest Diagnostics Investments Llc | Methods for detecting DNA mutations using mitra tip extraction |
| US11473144B2 (en) | 2016-11-15 | 2022-10-18 | Quest Diagnostics Investments Llc | Methods for detecting cystic fibrosis mutations using mitra tip extraction |
| EP3626835A1 (en) | 2018-09-18 | 2020-03-25 | Sistemas Genómicos, S.L. | Method for genotypically identifying both alleles of at least one locus of a subject's hla gene |
| RU2703401C1 (ru) * | 2018-10-24 | 2019-10-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Тест-система для выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней |
| RU2703394C1 (ru) * | 2018-10-24 | 2019-10-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Способ выявления и генотипирования РНК вируса репродуктивно-респираторного синдрома свиней |
| FR3100820B1 (fr) | 2019-09-12 | 2024-06-28 | Bionobis | Procédé de génotypage adapté au traitement d’un grand nombre d’échantillons, notamment en cas de polymorphisme élevé |
| FR3106596B1 (fr) | 2020-01-27 | 2022-12-02 | Bionobis | Procede de genotypage hla simple et rapide |
| CN111235266B (zh) * | 2020-03-10 | 2023-12-01 | 广州医科大学附属第二医院 | Hla亚型检测试剂盒及其应用 |
| FR3110178B1 (fr) * | 2020-05-18 | 2022-05-27 | Bionobis | Procédé de génotypage adapté au traitement simultané d’un grand nombre de patients |
| CN112553313A (zh) * | 2020-12-30 | 2021-03-26 | 武汉康圣达医学检验所有限公司 | 用于Sanger法的测序反应产物的变性方法 |
| KR102507110B1 (ko) * | 2022-02-15 | 2023-03-07 | 주식회사 네오젠티씨 | 주조직 적합성 복합체의 타입들을 분석하기 위한 방법 및 장치 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0656065A1 (en) | 1991-10-31 | 1995-06-07 | University Of Pittsburgh | Reverse dot blot hybridization using tandem head-to-tail monomers containing probes synthesized by staggered complementary primers |
| US5580730A (en) * | 1994-08-19 | 1996-12-03 | Olympus America, Inc. | Enzyme digestion method for the detection of amplified DNA |
| CA2280156C (en) * | 1997-02-11 | 2007-10-02 | William H. Hildebrand | Class i sequence based typing of hla-a, -b, and -c alleles by direct dna sequencing |
| EP0953650A1 (en) * | 1998-04-20 | 1999-11-03 | Innogenetics N.V. | Method for typing of HLA alleles |
| EP1594980B2 (en) * | 2003-01-29 | 2017-08-16 | 454 Life Sciences Corporation | Bead emulsion nucleic acid amplification |
| US7300755B1 (en) * | 2003-05-12 | 2007-11-27 | Fred Hutchinson Cancer Research Center | Methods for haplotyping genomic DNA |
| EP1711621A4 (en) * | 2003-10-28 | 2008-06-18 | Dynal Biotech Llc | PRIMERS, METHODS AND KITS FOR THE AMPLIFICATION OR DETECTION OF HUMAN LEUKOCYTE ANTIGEN ALLELES |
| EP1885882B1 (en) * | 2005-05-10 | 2011-01-26 | State of Oregon acting by & through the State Board of Higher Eduction on behalf of the University of Oregon | Methods of mapping polymorphisms and polymorphism microarrays |
| EP1915618A4 (en) * | 2005-06-02 | 2009-09-30 | Fluidigm Corp | ANALYSIS USING MICROFLUIDIC SEPARATION DEVICES |
| JP5237099B2 (ja) * | 2005-09-29 | 2013-07-17 | キージーン ナムローゼ フェンノートシャップ | 変異させた集団のハイスループットスクリーニング |
| ES2422288T3 (es) * | 2005-11-14 | 2013-09-10 | Keygene Nv | Método para el cribado ultrarrápido de poblaciones de transposón etiquetado e identificación masiva de la secuencia en paralelo de los sitios de inserción |
| EP2363504A1 (en) * | 2005-12-22 | 2011-09-07 | Keygene N.V. | Method for high-throughtput AFLP-based polymorphism detection |
| EP2035578A4 (en) * | 2006-06-09 | 2010-01-27 | Conexio 4 Pty Ltd | IDENTIFICATION OF A NUCLEIC ACID MOLECULE |
| US20100086914A1 (en) * | 2008-10-03 | 2010-04-08 | Roche Molecular Systems, Inc. | High resolution, high throughput hla genotyping by clonal sequencing |
| JP2011500041A (ja) * | 2007-10-16 | 2011-01-06 | エフ．ホフマン−ラ ロシュ アーゲー | クローナルシークエンシングによる高分解能かつ高効率のｈｌａ遺伝子型決定法 |
| RU2393231C1 (ru) * | 2008-12-29 | 2010-06-27 | Федеральное государственное учреждение здравоохранения "Российский научно-исследовательский противочумный институт "Микроб" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека ("РосНИПЧИ "Микроб") | Способ определения генетического родства штаммов холерных вибрионов методом секвенирования генов, фланкирующих кластер генов биосинтеза о-антигена |
| CN101921840B (zh) * | 2010-06-30 | 2014-06-25 | 深圳华大基因科技有限公司 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
| CN101921842B (zh) * | 2010-06-30 | 2013-08-07 | 深圳华大基因科技有限公司 | Hla-a,b基因分型用pcr引物及其使用方法 |
| CN101921841B (zh) | 2010-06-30 | 2014-03-12 | 深圳华大基因科技有限公司 | 基于Illumina GA测序技术的HLA基因高分辨率分型方法 |
-
2011
- 2011-06-30 KR KR1020137002332A patent/KR101709826B1/ko active Active
- 2011-06-30 BR BR112012032586-8A patent/BR112012032586B1/pt active IP Right Grant
- 2011-06-30 SG SG2012096616A patent/SG186876A1/en unknown
- 2011-06-30 EP EP11800190.8A patent/EP2599877B1/en active Active
- 2011-06-30 JP JP2013516983A patent/JP5968879B2/ja active Active
- 2011-06-30 CA CA2803940A patent/CA2803940C/en active Active
- 2011-06-30 DK DK11800190.8T patent/DK2599877T3/da active
- 2011-06-30 WO PCT/CN2011/076688 patent/WO2012000445A1/zh not_active Ceased
- 2011-06-30 RU RU2013103795/10A patent/RU2587606C2/ru active IP Right Revival
- 2011-06-30 US US13/807,660 patent/US9957564B2/en active Active
- 2011-06-30 MY MYPI2012005591A patent/MY173793A/en unknown
- 2011-06-30 AU AU2011274090A patent/AU2011274090B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2599877A1 (en) | 2013-06-05 |
| EP2599877B1 (en) | 2017-09-27 |
| KR20130038353A (ko) | 2013-04-17 |
| MY173793A (en) | 2020-02-24 |
| SG186876A1 (en) | 2013-02-28 |
| AU2011274090A1 (en) | 2013-02-07 |
| JP5968879B2 (ja) | 2016-08-10 |
| CA2803940A1 (en) | 2012-01-05 |
| WO2012000445A1 (zh) | 2012-01-05 |
| BR112012032586A2 (pt) | 2019-07-30 |
| AU2011274090B2 (en) | 2015-04-09 |
| BR112012032586B1 (pt) | 2021-08-17 |
| KR101709826B1 (ko) | 2017-02-24 |
| RU2013103795A (ru) | 2014-08-20 |
| DK2599877T3 (da) | 2017-11-20 |
| US9957564B2 (en) | 2018-05-01 |
| US20130237432A1 (en) | 2013-09-12 |
| EP2599877A4 (en) | 2014-01-01 |
| JP2013529472A (ja) | 2013-07-22 |
| RU2587606C2 (ru) | 2016-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2803940C (en) | Application of a pcr sequencing method, based on dna barcoding technique and dna incomplete shearing strategy, in hla genotyping | |
| Petersdorf et al. | A comprehensive approach for typing the alleles of the HLA‐B locus by automated sequencing | |
| CA2841060A1 (en) | Method and kit for dna typing of hla gene | |
| WO2012000150A1 (zh) | Hla-a，b基因分型用pcr引物及其使用方法 | |
| Lank et al. | A novel single cDNA amplicon pyrosequencing method for high-throughput, cost-effective sequence-based HLA class I genotyping | |
| WO2011030159A1 (en) | Method using hla epitope determination | |
| Bettinotti et al. | Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA | |
| CN103270170B (zh) | Hla-dqb1基因分型的方法及其相关引物 | |
| CN120060455B (zh) | 基于三代测序平台的hla基因扩增引物及其应用 | |
| TWI542696B (zh) | HLA - C genotyping and its related primers | |
| JP2009261358A (ja) | Ｈｌａ−ｄｒｂ１遺伝子を含むヒト遺伝子のアレル多型のタイピング方法及びこれに用いるキット | |
| WO2017135396A1 (ja) | Ｐｃｒを用いないキャプチャー法によるｈｌａ遺伝子タイピング用プローブセット及びそれを用いたタイピング方法 | |
| WO1998026091A2 (en) | Method and kit for hla class i typing | |
| Moran et al. | Identification of the MICA∗ 070 allele by sequencing and phasing | |
| KR102393484B1 (ko) | 식방풍 자원의 유전자형 판별을 위한 핵 게놈 서열 기반 분자마커 및 이의 용도 | |
| Quan et al. | Full‐length sequence of the novel HLA‐C* 03: 566 allele by next‐generation sequencing in a Chinese individual. | |
| Gao et al. | The full‐length genomic sequence of a novel HLA‐C* 07 allele, HLA‐C* 07: 63, identified in a Chinese individual. | |
| De Santis et al. | KIR Genotyping | |
| JP2010162020A (ja) | 新規ｈｌａ−ｄｒｂ１遺伝子およびその用途 | |
| HK1185115B (en) | Method for hla-dqb1 genotyping and related primers thereof | |
| HK1185114B (en) | Method for hla-c genotyping and related primers thereof | |
| Zou et al. | Identification of a novel HLA-B* 15 allele, HLA-B* 15: 374, in a Chinese individual. | |
| Chang et al. | Identification of a novel HLA‐A allele, A* 3120 | |
| KR20190021750A (ko) | 중증 열성 혈소판 감소 증후군 바이러스의 감염을 진단하기 위한 프라이머 세트, 이를 포함하는 진단용 키트 및 방법 | |
| JP2011050377A (ja) | 新規ｈｌａ−ｄｒｂ１遺伝子およびその用途 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20160428 |