CA2803933C - Method for analyzing biological specimens by spectral imaging - Google Patents
Method for analyzing biological specimens by spectral imaging Download PDFInfo
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- CA2803933C CA2803933C CA2803933A CA2803933A CA2803933C CA 2803933 C CA2803933 C CA 2803933C CA 2803933 A CA2803933 A CA 2803933A CA 2803933 A CA2803933 A CA 2803933A CA 2803933 C CA2803933 C CA 2803933C
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Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9025850B2 (en) * | 2010-06-25 | 2015-05-05 | Cireca Theranostics, Llc | Method for analyzing biological specimens by spectral imaging |
| US9129371B2 (en) | 2010-06-25 | 2015-09-08 | Cireca Theranostics, Llc | Method for analyzing biological specimens by spectral imaging |
| AU2012309158B2 (en) | 2011-06-24 | 2016-06-30 | Northeastern University | Phase correction to compensate for reflective distortions of optical spectra |
| US8725237B2 (en) | 2012-03-19 | 2014-05-13 | Genetic Innovations, Inc. | Devices, systems, and methods for virtual staining |
| JP5861225B2 (ja) * | 2012-06-07 | 2016-02-16 | 国立大学法人鳥取大学 | 被検サンプル組織体中の検体細胞の有無判別方法および装置 |
| JP6006036B2 (ja) * | 2012-08-17 | 2016-10-12 | オリンパス株式会社 | 分光スペクトル解析方法 |
| US9798918B2 (en) | 2012-10-05 | 2017-10-24 | Cireca Theranostics, Llc | Method and system for analyzing biological specimens by spectral imaging |
| WO2014153423A2 (en) * | 2013-03-19 | 2014-09-25 | Cireca Theranostics, Llc | Method and system for analyzing biological specimens by spectral imaging |
| US9364071B2 (en) * | 2013-09-19 | 2016-06-14 | Spectral Sensors Inc. | Systems and methods for measuring spectra of skin and other objects and materials and making predictions based thereon |
| US9269014B2 (en) | 2013-09-24 | 2016-02-23 | Corning Incorporated | Hyperspectral detector systems and methods using context-image fusion |
| CN103489194A (zh) * | 2013-09-30 | 2014-01-01 | 河海大学 | 基于安全半监督支持向量机的遥感影像变化检测方法 |
| EP3053139B1 (en) | 2013-10-01 | 2020-10-21 | Ventana Medical Systems, Inc. | Line-based image registration and cross-image annotation devices, systems and methods |
| WO2015128946A1 (ja) | 2014-02-25 | 2015-09-03 | オリンパス株式会社 | 分光スペクトル解析方法 |
| JP2015178986A (ja) * | 2014-03-19 | 2015-10-08 | 株式会社島津製作所 | 赤外顕微鏡 |
| JP6451741B2 (ja) * | 2014-07-11 | 2019-01-16 | 株式会社ニコン | 画像解析装置、撮像システム、手術支援システム、及び画像解析プログラム |
| RU2705389C2 (ru) * | 2014-07-21 | 2019-11-07 | 7386819 Манитоба Лтд. | Способ и устройство для сканирования костей в мясе |
| JP2016035394A (ja) * | 2014-08-01 | 2016-03-17 | パイオニア株式会社 | テラヘルツ波撮像装置及びテラヘルツ波撮像方法 |
| CA2968347A1 (en) * | 2014-11-21 | 2016-05-26 | Kyoto Stem Cell Innovation, Inc. | Quality evaluation method and quality evaluation device |
| KR101735464B1 (ko) * | 2014-12-17 | 2017-05-15 | 한밭대학교 산학협력단 | 유기물의 존재 또는 생물의 생사 측정 장치 및 방법 |
| US10460439B1 (en) | 2015-08-12 | 2019-10-29 | Cireca Theranostics, Llc | Methods and systems for identifying cellular subtypes in an image of a biological specimen |
| GB2545877B (en) * | 2015-09-10 | 2021-09-15 | Sierra Medical Ltd | ATR-FTIR computational analysis of Barrett's esophagus and esophageal cancers |
| KR101793609B1 (ko) * | 2015-09-11 | 2017-11-06 | 연세대학교 산학협력단 | 다중 광학 융합영상 기반 실시간으로 뇌종양을 진단하는 방법 및 장치 |
| JP6676160B2 (ja) * | 2015-10-28 | 2020-04-08 | ベンタナ メディカル システムズ， インコーポレイテッド | 中赤外分光法を用いて組織品質を分析するための方法およびシステム |
| JP6776672B2 (ja) | 2016-07-08 | 2020-10-28 | 住友電気工業株式会社 | 品質評価方法及び品質評価装置 |
| JP6422616B1 (ja) * | 2016-12-22 | 2018-11-14 | 国立大学法人 筑波大学 | データ作成方法及びデータ使用方法 |
| JP6975418B2 (ja) * | 2017-03-24 | 2021-12-01 | 株式会社Ｓｃｒｅｅｎホールディングス | 領域判定方法 |
| US11506881B2 (en) | 2017-05-22 | 2022-11-22 | La Trobe University | Method of imaging an object and a sample holder for use in an optical microscope |
| AU2018313841B2 (en) * | 2017-08-09 | 2023-10-26 | Allen Institute | Systems, devices, and methods for image processing to generate an image having predictive tagging |
| EP3685144A4 (en) * | 2017-09-21 | 2021-07-07 | Vital Biosciences Inc. | IMAGING OF BIOLOGICAL TISSUE OR OTHER SUBJECTS |
| JPWO2019073666A1 (ja) * | 2017-10-11 | 2020-12-03 | 株式会社ニコン | 判定装置、判定方法、および判定プログラム |
| US11321881B2 (en) | 2017-12-01 | 2022-05-03 | Sony Corporation | Image coloring apparatus, image coloring method, image learning apparatus, image learning method, computer program, and image coloring system |
| CN117958872A (zh) | 2017-12-05 | 2024-05-03 | Devicor医疗产业收购公司 | 具有应用成像的活检装置 |
| JP6951753B2 (ja) * | 2018-03-27 | 2021-10-20 | エバ・ジャパン 株式会社 | 情報探索システム及びプログラム |
| JP6669189B2 (ja) * | 2018-04-09 | 2020-03-18 | 株式会社島津製作所 | 赤外顕微鏡 |
| JP7235272B2 (ja) * | 2018-05-16 | 2023-03-08 | 株式会社アドダイス | 画像処理装置及び検査システム |
| CN109087341B (zh) * | 2018-06-07 | 2022-07-05 | 华南农业大学 | 一种近距离高光谱相机与测距传感器的融合方法 |
| GB2577349B (en) * | 2018-09-18 | 2023-06-14 | Univ Oxford Innovation Ltd | Radiomic signature of adipose |
| GB201817092D0 (en) * | 2018-10-19 | 2018-12-05 | Cancer Research Tech Ltd | Apparatus and method for wide-field hyperspectral imaging |
| SG11202105294PA (en) * | 2018-11-29 | 2021-06-29 | Univ La Trobe | Automated method of identifying a structure |
| JP7473223B2 (ja) * | 2019-07-24 | 2024-04-23 | 学校法人 埼玉医科大学 | 推定器学習装置、推定器学習方法、及び、推定器学習プログラム |
| KR102091832B1 (ko) * | 2019-08-05 | 2020-03-20 | 주식회사 에프앤디파트너스 | 멀티미디어 정보를 이용한 휴대용 체외진단키트 해석장치 |
| CN114270174A (zh) * | 2019-08-28 | 2022-04-01 | 文塔纳医疗系统公司 | 使用振动光谱对生物标志物表达进行无标记评估 |
| KR102347442B1 (ko) * | 2019-11-08 | 2022-01-05 | 한국광기술원 | 중적외선을 이용한 암종 컬러검출장치 및 방법 |
| KR102676218B1 (ko) * | 2019-12-16 | 2024-06-19 | (주)미래컴퍼니 | 테라헤르츠파를 이용한 검사 시스템 |
| KR102648081B1 (ko) * | 2019-12-13 | 2024-03-18 | (주)미래컴퍼니 | 테라헤르츠파를 이용한 검사 시스템 |
| KR102648099B1 (ko) * | 2019-12-16 | 2024-03-18 | (주)미래컴퍼니 | 테라헤르츠파를 이용한 검사 시스템 |
| KR102648092B1 (ko) * | 2019-12-16 | 2024-03-18 | (주)미래컴퍼니 | 테라헤르츠파를 이용한 검사 시스템 |
| KR102697403B1 (ko) * | 2019-12-16 | 2024-08-22 | (주)미래컴퍼니 | 테라헤르츠파를 이용한 검사 시스템 |
| GB202006494D0 (en) * | 2020-05-01 | 2020-06-17 | Imperial College Innovations Ltd | Processing 1h-nmr spectral data |
| JP2020204624A (ja) * | 2020-09-18 | 2020-12-24 | パイオニア株式会社 | テラヘルツ波撮像装置及びテラヘルツ波撮像方法 |
| KR102441907B1 (ko) * | 2021-07-08 | 2022-09-08 | 가톨릭대학교 산학협력단 | 암 진단을 위한 이미지 분류 장치 및 이미지 분류 방법 |
| WO2023096971A1 (en) * | 2021-11-24 | 2023-06-01 | Applied Materials, Inc. | Artificial intelligence-based hyperspectrally resolved detection of anomalous cells |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2519892A (en) * | 1991-08-20 | 1993-03-16 | Douglas C.B. Redd | Optical histochemical analysis, in vivo detection and real-time guidance for ablation of abnormal tissues using a raman spectroscopic detection system |
| US5713364A (en) * | 1995-08-01 | 1998-02-03 | Medispectra, Inc. | Spectral volume microprobe analysis of materials |
| US6826422B1 (en) * | 1997-01-13 | 2004-11-30 | Medispectra, Inc. | Spectral volume microprobe arrays |
| US6037772A (en) * | 1998-01-06 | 2000-03-14 | Arch Development Corp. | Fast spectroscopic imaging system |
| JPH11283019A (ja) * | 1998-03-31 | 1999-10-15 | Fuji Photo Film Co Ltd | 画像解析装置 |
| US6002476A (en) * | 1998-04-22 | 1999-12-14 | Chemicon Inc. | Chemical imaging system |
| US20030026762A1 (en) * | 1999-05-05 | 2003-02-06 | Malmros Mark K. | Bio-spectral imaging system and methods for diagnosing cell disease state |
| US6215554B1 (en) * | 1999-08-26 | 2001-04-10 | Yi Zhang | Laser diagnostic unit for detecting carcinosis |
| IL132687A0 (en) * | 1999-11-01 | 2001-03-19 | Keren Mechkarim Ichilov Pnimit | System and method for evaluating body fluid samples |
| US7755757B2 (en) * | 2007-02-14 | 2010-07-13 | Chemimage Corporation | Distinguishing between renal oncocytoma and chromophobe renal cell carcinoma using raman molecular imaging |
| JP2004053498A (ja) * | 2002-07-23 | 2004-02-19 | Matsushita Electric Ind Co Ltd | 顕微鏡画像解析装置とその画像解析方法 |
| JP2005058378A (ja) * | 2003-08-08 | 2005-03-10 | Olympus Corp | 手術用観察システム |
| US20060281068A1 (en) * | 2005-06-09 | 2006-12-14 | Chemimage Corp. | Cytological methods for detecting a disease condition such as malignancy by Raman spectroscopic imaging |
| JP4673000B2 (ja) * | 2004-05-21 | 2011-04-20 | 株式会社キーエンス | 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器 |
| JP2006026015A (ja) * | 2004-07-14 | 2006-02-02 | Fuji Photo Film Co Ltd | 光断層画像取得システム |
| EP1844426A4 (en) * | 2005-02-01 | 2016-09-07 | Amnis Corp | BLOOD ANALYSIS BY MEANS OF FLOW IMAGING CYTOMETER |
| JP4669330B2 (ja) * | 2005-06-17 | 2011-04-13 | 日本放送協会 | ２次元再生等化器および２次元再生等化方法 |
| US8081304B2 (en) * | 2006-07-31 | 2011-12-20 | Visualant, Inc. | Method, apparatus, and article to facilitate evaluation of objects using electromagnetic energy |
| JP5135882B2 (ja) * | 2007-05-22 | 2013-02-06 | 株式会社豊田中央研究所 | 物体識別装置及びプログラム |
| ES2438093T3 (es) * | 2008-05-29 | 2014-01-15 | Northeastern University | Método de reconstitución de espectros celulares útiles para la detección de trastornos celulares |
| JP5146667B2 (ja) * | 2008-06-13 | 2013-02-20 | 花王株式会社 | 肌のなめらかさの評価方法 |
| US9117133B2 (en) * | 2008-06-18 | 2015-08-25 | Spectral Image, Inc. | Systems and methods for hyperspectral imaging |
| JPWO2010046968A1 (ja) * | 2008-10-21 | 2012-03-15 | 西日本高速道路エンジニアリング四国株式会社 | コンクリート構造物の診断装置および診断方法 |
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| BR112012033200A2 (pt) | 2016-12-06 |
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