CA2797440C - Method of analysing a cell or other biological material containing a nucleic acid - Google Patents

Method of analysing a cell or other biological material containing a nucleic acid Download PDF

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Publication number
CA2797440C
CA2797440C CA2797440A CA2797440A CA2797440C CA 2797440 C CA2797440 C CA 2797440C CA 2797440 A CA2797440 A CA 2797440A CA 2797440 A CA2797440 A CA 2797440A CA 2797440 C CA2797440 C CA 2797440C
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Canada
Prior art keywords
compound
formula
cell
cells
fluorochrome
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CA2797440A
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English (en)
French (fr)
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CA2797440A1 (en
Inventor
Paul James Smith
Rachel J. Errington
Laurence Hylton Patterson
Original Assignee
Biostatus Ltd
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Publication of CA2797440A1 publication Critical patent/CA2797440A1/en
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Publication of CA2797440C publication Critical patent/CA2797440C/en
Active legal-status Critical Current
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
    • C09B1/16—Amino-anthraquinones
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
    • C09B1/16—Amino-anthraquinones
    • C09B1/20—Preparation from starting materials already containing the anthracene nucleus
    • C09B1/207—Dyes with amino groups and with onium groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent materials, e.g. electroluminescent or chemiluminescent
    • C09K11/06—Luminescent materials, e.g. electroluminescent or chemiluminescent containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1011—Condensed systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Materials Engineering (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2797440A 2010-04-09 2011-04-08 Method of analysing a cell or other biological material containing a nucleic acid Active CA2797440C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1005939.2A GB201005939D0 (en) 2010-04-09 2010-04-09 Method of analysing a cell or other biological material containing nucleic acid
GB1005939.2 2010-04-09
PCT/GB2011/050702 WO2011124927A1 (en) 2010-04-09 2011-04-08 Method of analysing a cell or other biological material containing a nucleic acid

Publications (2)

Publication Number Publication Date
CA2797440A1 CA2797440A1 (en) 2011-10-13
CA2797440C true CA2797440C (en) 2020-01-28

Family

ID=42236073

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2797440A Active CA2797440C (en) 2010-04-09 2011-04-08 Method of analysing a cell or other biological material containing a nucleic acid

Country Status (16)

Country Link
US (2) US9051467B2 (enExample)
EP (2) EP3366667B1 (enExample)
JP (2) JP6033767B2 (enExample)
KR (2) KR101957288B1 (enExample)
CN (1) CN102947266B (enExample)
AU (2) AU2011236610B2 (enExample)
BR (1) BR112012025820B1 (enExample)
CA (1) CA2797440C (enExample)
DK (2) DK3366667T3 (enExample)
ES (1) ES2676351T3 (enExample)
GB (1) GB201005939D0 (enExample)
HU (1) HUE038706T2 (enExample)
NO (1) NO2556047T3 (enExample)
PL (1) PL2556047T3 (enExample)
PT (1) PT2556047T (enExample)
WO (1) WO2011124927A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
WO2012138805A2 (en) 2011-04-04 2012-10-11 Biotium, Inc. Substituted anthraquinone dyes for cellular stains and enzyme detection
JP2013094135A (ja) * 2011-11-02 2013-05-20 Waseda Univ 人工ナノ材料の安全性を評価する方法
EP2834367A4 (en) * 2012-04-05 2016-03-23 Becton Dickinson Co SAMPLE PREPARATION FOR FLOW CYTOMETRY
US9645057B2 (en) 2012-04-05 2017-05-09 Becton, Dickiinson and Company Method for improving analysis of microorganisms in complex matrices
US10324036B2 (en) 2012-05-02 2019-06-18 Charles River Laboratories, Inc. Porous planar cell capture system
CN104755931B (zh) 2012-05-02 2016-08-31 查尔斯河实验室公司 用于检测细胞样品中的活细胞的方法
JP6285915B2 (ja) * 2012-05-02 2018-02-28 チャールズ リバー ラボラトリーズ， インコーポレイテッド 生死判別染色法
GB201214169D0 (en) 2012-08-08 2012-09-19 Biostatus Ltd New compounds and uses thereof
US20160021830A1 (en) * 2013-12-17 2016-01-28 The Penn State Research Foundation Manipulation of light spectral quality to reduce parasitism by cuscuta and other plant parasites
GB201610971D0 (en) 2016-06-23 2016-08-10 Biostatus Ltd Compounds
CN113667323B (zh) * 2019-11-21 2023-07-14 苏州优逸兰迪生物科技有限公司 一种染料化合物的制备方法
CN112679543B (zh) * 2020-12-29 2022-07-22 天津全和诚科技有限责任公司 一种亲脂性荧光素探针及其制备方法
CN113072937B (zh) * 2021-04-12 2022-06-07 郑州大学 一种脂滴靶向碳点、制备方法及应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3123605A (en) * 1960-01-08 1964-03-03 Basic anthraquinone dyestuffs
NL299891A (enExample) 1962-10-29
FR1379649A (fr) * 1963-06-20 1964-11-27 Oreal Nouveaux colorants anthraquinoniques et leur utilisation pour la teinture des cheveux
GB1053536A (enExample) * 1962-10-29
JPS503337B1 (enExample) * 1964-08-06 1975-02-03
US3646072A (en) * 1970-07-27 1972-02-29 Du Pont Turquoise biscationic anthraquinone dyes
JPS503337A (enExample) 1973-05-11 1975-01-14
US4123222A (en) 1976-03-23 1978-10-31 Ciba-Geigy Corporation Process for the dyeing or printing of polyacrylonitrile material
US4353708A (en) * 1978-07-13 1982-10-12 L'oreal Evaluation of the state of change of keratin fibers
FR2431128A1 (fr) * 1978-07-13 1980-02-08 Oreal Procede d'evaluation de l'etat ionique des cheveux
US5057413A (en) 1988-06-13 1991-10-15 Becton, Dickinson And Company Method for discriminating between intact and damaged cells in a sample
GB8923075D0 (en) 1989-10-13 1989-11-29 Patterson Laurence H Anti-cancer compounds
JPH04136802A (ja) * 1990-09-28 1992-05-11 Nippon Kayaku Co Ltd 着色フィルター
US5520707A (en) 1995-08-07 1996-05-28 Clairol, Inc. Methods for dyeing hair with anthraquinone hair dyes having a quaternary ammonium side chain
GB9813062D0 (en) 1998-06-18 1998-08-19 Univ Wales Medicine An anthraquinone and its derivatives
AU758538B2 (en) * 1998-12-07 2003-03-27 University Of South Carolina Research Foundation A colorant composition, a polymer nanocomposite comprising the colorant composition and articles produced therefrom
US6562785B1 (en) 1999-03-23 2003-05-13 Howard M. Shapiro Method for overcoming bacterial antibiotic resistance
CN1222518C (zh) * 2000-02-28 2005-10-12 第一化学药品株式会社 锌荧光探针
US6949250B2 (en) * 2002-12-16 2005-09-27 The United States Of America As Represented By The Secretary Of Agriculture Selective algaecides for control of cyanochloronta
US20070082377A1 (en) 2005-10-07 2007-04-12 Olin Michael R Cytotoxicity assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid

Also Published As

Publication number Publication date
PL2556047T3 (pl) 2018-10-31
NO2556047T3 (enExample) 2018-11-03
EP3366667B1 (en) 2021-02-17
AU2016250384B2 (en) 2018-05-10
JP2017112988A (ja) 2017-06-29
GB201005939D0 (en) 2010-05-26
BR112012025820B1 (pt) 2020-05-12
CN102947266B (zh) 2016-02-03
KR101846491B1 (ko) 2018-04-06
HUE038706T2 (hu) 2018-11-28
JP2013528571A (ja) 2013-07-11
DK2556047T3 (en) 2018-07-16
CA2797440A1 (en) 2011-10-13
BR112012025820A2 (pt) 2016-06-28
AU2011236610B2 (en) 2016-07-28
KR101957288B1 (ko) 2019-03-12
US9051467B2 (en) 2015-06-09
CN102947266A (zh) 2013-02-27
JP6033767B2 (ja) 2016-11-30
KR20130083836A (ko) 2013-07-23
ES2676351T3 (es) 2018-07-18
DK3366667T3 (da) 2021-05-10
US11255761B2 (en) 2022-02-22
JP6586070B2 (ja) 2019-10-02
KR20180037318A (ko) 2018-04-11
AU2016250384A1 (en) 2016-11-17
EP2556047A1 (en) 2013-02-13
EP3366667A1 (en) 2018-08-29
US20150268142A1 (en) 2015-09-24
US20130101994A1 (en) 2013-04-25
WO2011124927A1 (en) 2011-10-13
PT2556047T (pt) 2018-07-11
EP2556047B1 (en) 2018-06-06

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