BR112012025820A2 - método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico - Google Patents

método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico

Info

Publication number
BR112012025820A2
BR112012025820A2 BR112012025820A BR112012025820A BR112012025820A2 BR 112012025820 A2 BR112012025820 A2 BR 112012025820A2 BR 112012025820 A BR112012025820 A BR 112012025820A BR 112012025820 A BR112012025820 A BR 112012025820A BR 112012025820 A2 BR112012025820 A2 BR 112012025820A2
Authority
BR
Brazil
Prior art keywords
analyzing
cell
nucleic acid
biological material
atom
Prior art date
Application number
BR112012025820A
Other languages
English (en)
Other versions
BR112012025820B1 (pt
Inventor
Rachel J Errington
Patterson Laurence Hylton
Smith Paul James
Original Assignee
Biostatus Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biostatus Ltd filed Critical Biostatus Ltd
Publication of BR112012025820A2 publication Critical patent/BR112012025820A2/pt
Publication of BR112012025820B1 publication Critical patent/BR112012025820B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
    • C09B1/16—Amino-anthraquinones
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
    • C09B1/16—Amino-anthraquinones
    • C09B1/20—Preparation from starting materials already containing the anthracene nucleus
    • C09B1/207—Dyes with amino groups and with onium groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1011—Condensed systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions

Abstract

método de analisar uma célula ou outro material biológical compreendendo um ácido nucleico. de acordo com a invenção, é fornecido um composto de f´romula (i) em que a é um grupo alquileno c~ 2~-~ 8~; r^ 1^, r^ 2^, r^ 3^ e r^ 4^ são independentemente selecionados de hidrogênio, alquila c~ 1~-~ 4~ e di-hidroxialquila c~ 2~-~ 4~, em que o átomo de carbono ligado ao átomo de hidrogênio não é substituído com um grupo hidroxila e nenhum átomo de carbono é subsbtituído com dois grupos hidroxila, ou r^ 2^ e r^ 3^ juntos formam um grupo alquileno c~ 2~-~ 6~, que com o átomo de nitrogênio ao qual r^ 2^ e r^ 3^estão ligados formam um anel heterocíclico; x~ 1~, x~ 2~ e x~ 3~ são independentemente selecionados de hidrogênio, hidroxila, nr^ 1^-a- nr^ 2^r^ 3^r^ 4^ + (z^ m-^) 1/m, amino halogênio, alcóxi c~ 1~-~ 4~ ou alcanoiloxi c~ 2~-~ 8~; e (z^ m-^) 1/m é um ânion de carga m; ou um derivado cujo grupo nr^ 1^ é queternizado.
BR112012025820-6A 2010-04-09 2011-04-08 Composto, composição e complexo fluorescente compreendendo o mesmo, método de fabricação do composto e método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico BR112012025820B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1005939.2 2010-04-09
GBGB1005939.2A GB201005939D0 (en) 2010-04-09 2010-04-09 Method of analysing a cell or other biological material containing nucleic acid
PCT/GB2011/050702 WO2011124927A1 (en) 2010-04-09 2011-04-08 Method of analysing a cell or other biological material containing a nucleic acid

Publications (2)

Publication Number Publication Date
BR112012025820A2 true BR112012025820A2 (pt) 2016-06-28
BR112012025820B1 BR112012025820B1 (pt) 2020-05-12

Family

ID=42236073

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012025820-6A BR112012025820B1 (pt) 2010-04-09 2011-04-08 Composto, composição e complexo fluorescente compreendendo o mesmo, método de fabricação do composto e método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico

Country Status (16)

Country Link
US (2) US9051467B2 (pt)
EP (2) EP3366667B1 (pt)
JP (2) JP6033767B2 (pt)
KR (2) KR101846491B1 (pt)
CN (1) CN102947266B (pt)
AU (2) AU2011236610B2 (pt)
BR (1) BR112012025820B1 (pt)
CA (1) CA2797440C (pt)
DK (2) DK2556047T3 (pt)
ES (1) ES2676351T3 (pt)
GB (1) GB201005939D0 (pt)
HU (1) HUE038706T2 (pt)
NO (1) NO2556047T3 (pt)
PL (1) PL2556047T3 (pt)
PT (1) PT2556047T (pt)
WO (1) WO2011124927A1 (pt)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US8790892B2 (en) 2011-04-04 2014-07-29 Biotium, Inc. Substituted anthraquinone dyes for cellular stains and enzyme detection
JP2013094135A (ja) * 2011-11-02 2013-05-20 Waseda Univ 人工ナノ材料の安全性を評価する方法
US9645057B2 (en) 2012-04-05 2017-05-09 Becton, Dickiinson and Company Method for improving analysis of microorganisms in complex matrices
IN2014DN08957A (pt) * 2012-04-05 2015-05-22 Becton Dickinson Co
US8993260B2 (en) * 2012-05-02 2015-03-31 Charles River Laboratories, Inc. Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
US10324036B2 (en) 2012-05-02 2019-06-18 Charles River Laboratories, Inc. Porous planar cell capture system
GB201214169D0 (en) 2012-08-08 2012-09-19 Biostatus Ltd New compounds and uses thereof
WO2015095152A1 (en) * 2013-12-17 2015-06-25 The Penn State Research Foundation Manipulation of light spectral quality to reduce parasitism by cuscuta and other plant parasites
GB201610971D0 (en) 2016-06-23 2016-08-10 Biostatus Ltd Compounds
CN111117284B (zh) * 2019-11-21 2021-08-06 苏州优逸兰迪生物科技有限公司 RedNucleusⅡ染料及其制备方法与基于该染料的细胞凋亡检测试剂盒
CN112679543B (zh) * 2020-12-29 2022-07-22 天津全和诚科技有限责任公司 一种亲脂性荧光素探针及其制备方法
CN113072937B (zh) * 2021-04-12 2022-06-07 郑州大学 一种脂滴靶向碳点、制备方法及应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3123605A (en) * 1960-01-08 1964-03-03 Basic anthraquinone dyestuffs
NL126022C (pt) * 1962-10-29
NL299891A (pt) * 1962-10-29
FR1379649A (fr) * 1963-06-20 1964-11-27 Oreal Nouveaux colorants anthraquinoniques et leur utilisation pour la teinture des cheveux
JPS503337B1 (pt) * 1964-08-06 1975-02-03
US3646072A (en) * 1970-07-27 1972-02-29 Du Pont Turquoise biscationic anthraquinone dyes
JPS503337A (pt) 1973-05-11 1975-01-14
US4123222A (en) 1976-03-23 1978-10-31 Ciba-Geigy Corporation Process for the dyeing or printing of polyacrylonitrile material
FR2431128A1 (fr) * 1978-07-13 1980-02-08 Oreal Procede d'evaluation de l'etat ionique des cheveux
US4353708A (en) * 1978-07-13 1982-10-12 L'oreal Evaluation of the state of change of keratin fibers
US5057413A (en) * 1988-06-13 1991-10-15 Becton, Dickinson And Company Method for discriminating between intact and damaged cells in a sample
GB8923075D0 (en) 1989-10-13 1989-11-29 Patterson Laurence H Anti-cancer compounds
JPH04136802A (ja) * 1990-09-28 1992-05-11 Nippon Kayaku Co Ltd 着色フィルター
US5520707A (en) 1995-08-07 1996-05-28 Clairol, Inc. Methods for dyeing hair with anthraquinone hair dyes having a quaternary ammonium side chain
GB9813062D0 (en) * 1998-06-18 1998-08-19 Univ Wales Medicine An anthraquinone and its derivatives
WO2000034379A1 (en) * 1998-12-07 2000-06-15 Eastman Chemical Company A colorant composition, a polymer nanocomposite comprising the colorant composition and articles produced therefrom
US6562785B1 (en) * 1999-03-23 2003-05-13 Howard M. Shapiro Method for overcoming bacterial antibiotic resistance
EP1260510B1 (en) * 2000-02-28 2006-10-18 Daiichi Pure Chemicals Co., Ltd. Fluorescent probes for the quantitation of zinc
US6949250B2 (en) 2002-12-16 2005-09-27 The United States Of America As Represented By The Secretary Of Agriculture Selective algaecides for control of cyanochloronta
US20070082377A1 (en) 2005-10-07 2007-04-12 Olin Michael R Cytotoxicity assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid

Also Published As

Publication number Publication date
AU2016250384B2 (en) 2018-05-10
EP2556047B1 (en) 2018-06-06
JP6586070B2 (ja) 2019-10-02
AU2016250384A1 (en) 2016-11-17
US20130101994A1 (en) 2013-04-25
ES2676351T3 (es) 2018-07-18
CN102947266A (zh) 2013-02-27
CA2797440C (en) 2020-01-28
CA2797440A1 (en) 2011-10-13
US20150268142A1 (en) 2015-09-24
EP3366667A1 (en) 2018-08-29
GB201005939D0 (en) 2010-05-26
AU2011236610B2 (en) 2016-07-28
KR20130083836A (ko) 2013-07-23
HUE038706T2 (hu) 2018-11-28
KR101957288B1 (ko) 2019-03-12
BR112012025820B1 (pt) 2020-05-12
CN102947266B (zh) 2016-02-03
DK2556047T3 (en) 2018-07-16
NO2556047T3 (pt) 2018-11-03
JP2017112988A (ja) 2017-06-29
EP3366667B1 (en) 2021-02-17
WO2011124927A1 (en) 2011-10-13
PL2556047T3 (pl) 2018-10-31
JP2013528571A (ja) 2013-07-11
DK3366667T3 (da) 2021-05-10
US11255761B2 (en) 2022-02-22
PT2556047T (pt) 2018-07-11
EP2556047A1 (en) 2013-02-13
US9051467B2 (en) 2015-06-09
JP6033767B2 (ja) 2016-11-30
KR101846491B1 (ko) 2018-04-06
KR20180037318A (ko) 2018-04-11

Similar Documents

Publication Publication Date Title
BR112012025820A2 (pt) método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico
BR112014010644A2 (pt) novos derivados de aril-quinolina
CO6700850A2 (es) Estabilizadores de luz de amina estéricamente impedida con funcionalización mixta
TR201900038T4 (tr) GPR6'nın tetrahidropiridopirazin modülatörleri.
MX2013006101A (es) Compuestos heterociclicos nitrogenados sustituidos fusionados en posicion 6,6 y usos de los mismos.
BR112014006660A2 (pt) novos derivados de dihidroquinolina-2-ona bicíclicos
MX356028B (es) Compuesto adyuvante.
EA201390633A1 (ru) Новые вещества для повторно перерабатываемых эпоксидных смол
BR112012026950A2 (pt) composto, e, uso de um composto
UA111626C2 (uk) Похідні дигідрохінолін-2-ону
AR083873A1 (es) Derivados de acido amino-fenil-pentanoico sustituidos como inhibidores de nep
MX2013004242A (es) Sulfonamidas nematocidas.
CO7111313A2 (es) Compuestos de n-(tetrazol-5-il)- y n-(triazol-5-il)arilcarboxamida sustituidos y su uso como herbicidas
BR112014015905A2 (pt) composição
AR080596A1 (es) Compuestos alquilamido y composiciones farmaceuticas
BR112012007411B8 (pt) composto, processo para sua preparação e composição farmacêutica contendo o mesmo
MX346746B (es) Compuestos de anillo indeno-fusionados.
BR112015009034A2 (pt) método para a preparação de uma suspensão aquosa, suspensão aquosa, e, uso de uma suspensão aquosa
MX2011007952A (es) Tricianoboratos novedosos.
TR201905537T4 (tr) Antibakteriyel siklopenta[c]pirol sübstitüe 3,4-dihidro-1H-[1,8] naftiridinonlar.
MY161134A (en) Piperazine compound having a pgds inhibitory effect
EA201490436A1 (ru) Антибактериальные 3,4-дигидро-1н-[1,8]нафтиридиноны, замещенные гомопиперидинилом
ECSP13012631A (es) Estabilizadores de luz de amina estéricamente impedida con funcionalización mixta
BR112021026899A2 (pt) Compostos heterocíclicos
BR112013016431A2 (pt) novos derivados de amidas de biarila

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]

Free format text: SOLICITA-SE PAGAMENTE DE TAXA DE EXAME REFERENTE A QUANTIDADE ADICIONAL DE REIVINDICACOES SUBMETIDA A EXAME.

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/04/2011, OBSERVADAS AS CONDICOES LEGAIS.