CA2598514C - Mutant aox 1 promoters - Google Patents

Mutant aox 1 promoters Download PDF

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Publication number
CA2598514C
CA2598514C CA2598514A CA2598514A CA2598514C CA 2598514 C CA2598514 C CA 2598514C CA 2598514 A CA2598514 A CA 2598514A CA 2598514 A CA2598514 A CA 2598514A CA 2598514 C CA2598514 C CA 2598514C
Authority
CA
Canada
Prior art keywords
cell
promoter
nucleotides
nucleic acid
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2598514A
Other languages
English (en)
French (fr)
Other versions
CA2598514A1 (en
Inventor
Franz Hartner
Anton Glieder
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VTU Holding GmbH
Technische Universitaet Graz
Original Assignee
VTU Holding GmbH
Technische Universitaet Graz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VTU Holding GmbH, Technische Universitaet Graz filed Critical VTU Holding GmbH
Priority to CA2785161A priority Critical patent/CA2785161C/en
Publication of CA2598514A1 publication Critical patent/CA2598514A1/en
Application granted granted Critical
Publication of CA2598514C publication Critical patent/CA2598514C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03013—Alcohol oxidase (1.1.3.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA2598514A 2005-02-23 2006-02-23 Mutant aox 1 promoters Expired - Lifetime CA2598514C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2785161A CA2785161C (en) 2005-02-23 2006-02-23 Mutant aox1 promoters

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ATA304/2005 2005-02-23
AT0030405A AT501955B1 (de) 2005-02-23 2005-02-23 Mutierte aox1-promotoren
PCT/AT2006/000079 WO2006089329A2 (en) 2005-02-23 2006-02-23 Mutant aox 1 promoters

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2785161A Division CA2785161C (en) 2005-02-23 2006-02-23 Mutant aox1 promoters

Publications (2)

Publication Number Publication Date
CA2598514A1 CA2598514A1 (en) 2006-08-31
CA2598514C true CA2598514C (en) 2013-07-09

Family

ID=36927772

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2598514A Expired - Lifetime CA2598514C (en) 2005-02-23 2006-02-23 Mutant aox 1 promoters
CA2785161A Expired - Lifetime CA2785161C (en) 2005-02-23 2006-02-23 Mutant aox1 promoters

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2785161A Expired - Lifetime CA2785161C (en) 2005-02-23 2006-02-23 Mutant aox1 promoters

Country Status (20)

Country Link
US (2) US9012175B2 (enExample)
EP (2) EP2199389B1 (enExample)
JP (2) JP5497986B2 (enExample)
KR (2) KR101352399B1 (enExample)
CN (2) CN101935657B (enExample)
AT (3) AT501955B1 (enExample)
AU (2) AU2006216094B2 (enExample)
BR (1) BRPI0606179B1 (enExample)
CA (2) CA2598514C (enExample)
DE (2) DE602006018113D1 (enExample)
DK (2) DK2199389T3 (enExample)
ES (2) ES2358086T3 (enExample)
HR (2) HRP20110084T2 (enExample)
IN (1) IN2012DN06694A (enExample)
NZ (2) NZ560542A (enExample)
PL (2) PL1851312T3 (enExample)
PT (1) PT1851312E (enExample)
SI (2) SI2199389T1 (enExample)
UA (2) UA94399C2 (enExample)
WO (1) WO2006089329A2 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5444323B2 (ja) * 2008-03-27 2014-03-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 高発現ザイモモナス（Ｚｙｍｏｍｏｎａｓ）プロモーター
EP2300615A2 (en) * 2008-07-11 2011-03-30 Novozymes A/S Pichia pastoris das promoter variants
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012129036A2 (en) * 2011-03-22 2012-09-27 Merck Sharp & Dohme Corp. Promoters for high level recombinant expression in fungal host cells
GB201113419D0 (en) * 2011-08-04 2011-09-21 Fujifilm Diosynth Biotechnologies Uk Ltd Yeast vector
EP2565262A1 (en) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
AU2012320421B2 (en) 2011-10-07 2017-03-02 Lonza Ltd Regulatable promoter
WO2014088563A1 (en) * 2012-12-05 2014-06-12 Exxonmobil Research And Engineering Company Nannochloropsis kozak consensus sequence
PL2964765T3 (pl) 2013-03-08 2019-10-31 Keck Graduate Inst Of Applied Life Sciences Promotory drożdżowe z pichia pastoris
DK2964775T3 (en) 2013-03-08 2019-04-15 Biogrammatics Inc Yeast promoters for protein expression
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
EP2970994B1 (en) 2013-03-15 2019-07-17 Lonza Ltd Constitutive promoter
CN114365792A (zh) 2014-11-11 2022-04-19 克莱拉食品公司 用于生成卵清蛋白的方法和组合物
HRP20220299T1 (hr) 2015-05-11 2022-05-13 Impossible Foods Inc. Ekspresijski konstrukti i postupci za genetsku modifikaciju metilotrofnog kvasca
WO2017021541A1 (en) 2015-08-05 2017-02-09 Lonza Ltd Promoter variants
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR102596200B1 (ko) * 2017-06-30 2023-10-30 다우 글로벌 테크놀로지스 엘엘씨 알데히드 개선을 위한 코팅 및 이의 제조 방법
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
WO2020068018A1 (en) * 2018-09-24 2020-04-02 Orta Dogu Teknik Universitesi Modified aox1 promoter variants
CN112739819A (zh) * 2018-09-24 2021-04-30 中东科技大学 利用启动子工程化设计醇脱氢酶2(adh2)启动子变体
CN109735547B (zh) * 2019-03-19 2022-05-31 青岛蔚蓝生物集团有限公司 一种提高毕赤酵母外源蛋白表达量的启动子及其应用
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
AU2020257270B2 (en) * 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
CN110106168B (zh) * 2019-05-27 2021-07-13 西南大学 基于转录因子间的互作关系构建双激素响应启动子的方法
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
ES3015627T3 (en) 2019-07-11 2025-05-06 Clara Foods Co Beverage composition comprising recombinant ovomucoid protein
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN110724720A (zh) * 2019-11-01 2020-01-24 山东仙普爱瑞科技股份有限公司 一种猪α-干扰素的发酵工艺
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
CN117120618A (zh) 2021-02-12 2023-11-24 勃林格殷格翰 Rcv 两合公司 用于增加蛋白质分泌的信号肽
US11512336B1 (en) * 2021-06-24 2022-11-29 BioBoost Synbio Consulting Inc. Method for methanol free culturing of methylotrophic yeast for the biosynthesis of added value products
CN121263531A (zh) 2023-06-06 2026-01-02 富士胶片株式会社 基因重组酵母、目标蛋白质的制造方法、试剂盒及基因重组酵母的制造方法
MX2025015008A (es) * 2023-06-15 2026-02-03 Danstar Ferment Ag Promotores que reaccionan a la fuente de carbono c2
CN117417934B (zh) * 2023-10-27 2024-09-13 江南大学 跨菌种启动子、多菌种穿梭质粒及其应用
WO2025104126A1 (en) 2023-11-14 2025-05-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2025163019A1 (en) 2024-02-01 2025-08-07 Boehringer Ingelheim Rcv Gmbh & Co Kg Tags for enhanced expression of recombinant proteins
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN118599913B (zh) * 2024-08-07 2024-12-27 南昌大学 醛氧化酶Aox1基因敲除动物模型的构建方法及应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2063890C (en) * 1991-03-27 2007-03-13 Masami Miura Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
JPH06209763A (ja) * 1993-01-13 1994-08-02 Green Cross Corp:The 変異株
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
JP3290108B2 (ja) * 1997-08-29 2002-06-10 麒麟麦酒株式会社 プロモーター活性を増大させるｄｎａ配列
CN1192103C (zh) * 1998-01-27 2005-03-09 三井化学株式会社 生产植酸酶的方法
AU3910000A (en) * 1999-03-22 2000-10-09 Zymogenetics Inc. Improved methods for producing proteins in transformed (pichia)
CN1188524C (zh) * 2001-02-28 2005-02-09 珠江医院 人SP－A1在pichia pastoris中的表达
WO2002081650A2 (en) * 2001-04-05 2002-10-17 The Board Of Regents Of The University Of Nebraska Alcohol oxidase 1 regulatory nucleotide sequences for heterologous gene expression in yeast
EP1431387B1 (en) * 2002-12-20 2008-02-27 Roche Diagnostics GmbH Heat-labile desoxyribonculease I variants

Also Published As

Publication number Publication date
AT501955B1 (de) 2007-08-15
KR20120106979A (ko) 2012-09-27
PT1851312E (pt) 2011-01-05
EP2199389A1 (en) 2010-06-23
HRP20110084T2 (hr) 2011-10-31
CN101935657A (zh) 2011-01-05
PL1851312T3 (pl) 2011-05-31
BRPI0606179A2 (pt) 2009-06-02
KR101358663B1 (ko) 2014-02-20
EP1851312A2 (en) 2007-11-07
WO2006089329A3 (en) 2007-05-31
EP1851312B9 (en) 2011-04-20
US9279129B2 (en) 2016-03-08
US9012175B2 (en) 2015-04-21
CA2785161C (en) 2020-03-31
CN101128581A (zh) 2008-02-20
ATE496125T1 (de) 2011-02-15
AU2006216094B2 (en) 2010-10-21
SI1851312T1 (sl) 2011-03-31
HK1113172A1 (en) 2008-09-26
UA98604C2 (ru) 2012-05-25
HK1148313A1 (en) 2011-09-02
AU2011200187B2 (en) 2012-01-19
CA2598514A1 (en) 2006-08-31
AU2011200187A1 (en) 2011-02-03
DK1851312T3 (da) 2011-02-28
JP5552107B2 (ja) 2014-07-16
AU2006216094A1 (en) 2006-08-31
KR101352399B1 (ko) 2014-02-18
WO2006089329A2 (en) 2006-08-31
AT501955A1 (de) 2006-12-15
BRPI0606179B1 (pt) 2018-05-29
IN2012DN06694A (enExample) 2015-10-23
UA94399C2 (ru) 2011-05-10
DK1851312T5 (da) 2011-04-18
ES2356127T9 (es) 2011-05-30
JP5497986B2 (ja) 2014-05-21
WO2006089329A8 (en) 2006-11-02
US20100196913A1 (en) 2010-08-05
NZ560542A (en) 2010-06-25
CA2785161A1 (en) 2006-08-31
JP2012080886A (ja) 2012-04-26
HRP20110084T1 (hr) 2011-03-31
HRP20110254T1 (hr) 2011-05-31
US20080153126A1 (en) 2008-06-26
ES2356127T3 (es) 2011-04-05
SI2199389T1 (sl) 2011-05-31
DE602006019789D1 (de) 2011-03-03
NZ585821A (en) 2011-02-25
EP2199389B1 (en) 2011-01-19
DK2199389T3 (da) 2011-04-11
EP1851312B1 (en) 2010-11-10
ES2358086T3 (es) 2011-05-05
KR20070107781A (ko) 2007-11-07
JP2008531000A (ja) 2008-08-14
CN101128581B (zh) 2012-04-18
PL2199389T3 (pl) 2011-06-30
ATE487788T1 (de) 2010-11-15
CN101935657B (zh) 2013-02-13
DE602006018113D1 (de) 2010-12-23

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