CA2484336C - Pulsed-multiline excitation for color-blind fluorescence detection - Google Patents

Pulsed-multiline excitation for color-blind fluorescence detection Download PDF

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Publication number
CA2484336C
CA2484336C CA2484336A CA2484336A CA2484336C CA 2484336 C CA2484336 C CA 2484336C CA 2484336 A CA2484336 A CA 2484336A CA 2484336 A CA2484336 A CA 2484336A CA 2484336 C CA2484336 C CA 2484336C
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CA
Canada
Prior art keywords
excitation
laser
sample
lasers
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2484336A
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English (en)
French (fr)
Other versions
CA2484336A1 (en
Inventor
Graham B. I. Scott
Carter Kittrell
Robert F. Curl
Michael L. Metzker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
William Marsh Rice University
Original Assignee
Baylor College of Medicine
William Marsh Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baylor College of Medicine, William Marsh Rice University filed Critical Baylor College of Medicine
Priority to CA2774152A priority Critical patent/CA2774152A1/en
Publication of CA2484336A1 publication Critical patent/CA2484336A1/en
Application granted granted Critical
Publication of CA2484336C publication Critical patent/CA2484336C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
    • G01J3/021—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using plane or convex mirrors, parallel phase plates, or particular reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2484336A 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection Expired - Fee Related CA2484336C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2774152A CA2774152A1 (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/941,165 US6995841B2 (en) 2001-08-28 2001-08-28 Pulsed-multiline excitation for color-blind fluorescence detection
US09/941,165 2001-08-28
PCT/US2002/027406 WO2003021212A1 (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2774152A Division CA2774152A1 (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection

Publications (2)

Publication Number Publication Date
CA2484336A1 CA2484336A1 (en) 2003-03-13
CA2484336C true CA2484336C (en) 2012-06-26

Family

ID=25476036

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2774152A Abandoned CA2774152A1 (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection
CA2484336A Expired - Fee Related CA2484336C (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2774152A Abandoned CA2774152A1 (en) 2001-08-28 2002-08-28 Pulsed-multiline excitation for color-blind fluorescence detection

Country Status (6)

Country Link
US (4) US6995841B2 (enExample)
EP (1) EP1432966A4 (enExample)
JP (3) JP2005524051A (enExample)
AU (1) AU2002323452C1 (enExample)
CA (2) CA2774152A1 (enExample)
WO (1) WO2003021212A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230324662A1 (en) * 2020-08-05 2023-10-12 Leica Microsystems Cms Gmbh Method for adjusting the illumination in a fluorescence microscope, and corresponding fluorescence microscope

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