CA2374009A1 - Glucoamylase variant - Google Patents

Glucoamylase variant Download PDF

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Publication number
CA2374009A1
CA2374009A1 CA002374009A CA2374009A CA2374009A1 CA 2374009 A1 CA2374009 A1 CA 2374009A1 CA 002374009 A CA002374009 A CA 002374009A CA 2374009 A CA2374009 A CA 2374009A CA 2374009 A1 CA2374009 A1 CA 2374009A1
Authority
CA
Canada
Prior art keywords
glucoamylase
variant
ser
amino acid
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002374009A
Other languages
English (en)
French (fr)
Inventor
Bjarne Ronfeldt Nielsen
Allan Svendsen
Henrik Pedersen
Jesper Vind
Hanne Vang Hendriksen
Torben Peter Frandsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2374009A1 publication Critical patent/CA2374009A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/20Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002374009A 1999-07-09 2000-07-07 Glucoamylase variant Abandoned CA2374009A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199900999 1999-07-09
DKPA199900999 1999-07-09
PCT/DK2000/000373 WO2001004273A2 (en) 1999-07-09 2000-07-07 Glucoamylase variant

Publications (1)

Publication Number Publication Date
CA2374009A1 true CA2374009A1 (en) 2001-01-18

Family

ID=8099879

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002374009A Abandoned CA2374009A1 (en) 1999-07-09 2000-07-07 Glucoamylase variant

Country Status (8)

Country Link
US (3) US7354753B2 (https=)
EP (2) EP2009098A1 (https=)
JP (1) JP2003504046A (https=)
CN (2) CN100510067C (https=)
AR (2) AR029759A1 (https=)
AU (1) AU5805700A (https=)
CA (1) CA2374009A1 (https=)
WO (1) WO2001004273A2 (https=)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6352851B1 (en) 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
CN100510067C (zh) * 1999-07-09 2009-07-08 诺维信公司 葡糖淀粉酶变体
EP2308980A3 (en) 2000-08-01 2011-04-27 Novozymes A/S Alpha-amylase mutants with altered properties
ATE449840T1 (de) 2001-05-15 2009-12-15 Novozymes As Alpha-amylasevariante mit veränderten eigenschaften
WO2003029449A2 (en) * 2001-10-01 2003-04-10 Novozymes A/S Glucoamylase variants
JP4897186B2 (ja) * 2002-03-27 2012-03-14 花王株式会社 変異アルカリセルラーゼ
DE60310264T2 (de) 2002-12-17 2007-07-05 Novozymes A/S Thermostabile alpha-amylase
EP1633878A1 (en) 2003-05-30 2006-03-15 Novozymes A/S Alcohol product processes
US8143048B2 (en) 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
CA2536376A1 (en) 2003-07-07 2005-01-27 Genencor International, Inc. Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
EP1687419B1 (en) 2003-10-28 2010-02-03 Novozymes North America, Inc. Hybrid enzymes
DE102004026152A1 (de) * 2004-05-28 2005-12-15 Basf Ag Fermentative Herstellung von Feinchemikalien
KR101336659B1 (ko) * 2004-07-07 2013-12-04 다니스코 유에스 인크. 폴리펩타이드
EP1831384B1 (en) 2004-12-22 2015-08-12 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US8030050B2 (en) 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species
CN101268195A (zh) 2005-09-20 2008-09-17 诺维信北美公司 产生发酵产物的方法
US20080318284A1 (en) 2005-12-22 2008-12-25 Novozymes North America, Inc. Processes for Producing a Fermentation Product
CN100443589C (zh) * 2005-12-30 2008-12-17 湖南鸿鹰祥生物工程股份有限公司 高转化率高纯度糖化酶浓缩液的生产方法
CN101405397A (zh) 2006-03-22 2009-04-08 诺维信北美公司 发酵方法
CN101528769B (zh) 2006-06-19 2015-09-30 杜邦营养生物科学有限公司 多肽
EP2061882A2 (en) 2006-10-10 2009-05-27 Danisco US, INC., Genencor Division Glucoamylase variants with altered properties
DK3219804T3 (da) 2007-04-24 2019-09-30 Novozymes North America Inc Afgiftning af forbehandlede lignocelluloseholdige materialer
US9695549B2 (en) 2007-09-03 2017-07-04 Norozymes Als Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
ES2502743T3 (es) * 2007-10-09 2014-10-06 Danisco Us Inc. Variantes de glucoamilasa
ES2527586T3 (es) * 2007-11-20 2015-01-27 Danisco Us Inc. Variantes de glucoamilasa con propiedades modificadas
WO2009129086A2 (en) * 2008-04-18 2009-10-22 New England Biolabs, Inc. Endoglycosidases that cleave o-linked glycans
MX2010013113A (es) 2008-06-06 2010-12-21 Danisco Inc Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas.
US20110097779A1 (en) 2008-06-23 2011-04-28 Chee-Leong Soong Processes for Producing Fermentation Products
DK2344650T3 (da) 2008-09-30 2014-07-14 Novozymes North America Inc Forbedring af enzymatisk hydrolyse af forbehandlet lignocelluloseholdigt materiale med distillers dried grain med solubles (ddg/s)
EP2857515B1 (en) 2008-11-20 2018-02-21 Novozymes Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
WO2010078391A2 (en) 2008-12-30 2010-07-08 Novozymes North America, Inc. Improvement of enzymatic hydrolysis of pretreated lignocellulose-containing material with dissolved air flotation sludge
WO2010078392A2 (en) 2008-12-31 2010-07-08 Novozymes North America, Inc. Processes of producing fermentation products
EP2451961A1 (en) 2009-07-07 2012-05-16 Novozymes A/S Process for treating a substrate with an enzyme
MX2012000322A (es) 2009-07-17 2012-02-08 Novozymes As Metodo de analisis del decaimiento de la celulosa en la hidrolisis del material celulosico.
AU2010284963C1 (en) * 2009-08-19 2014-11-27 Danisco Us Inc. Variants of glucoamylase
WO2011039324A1 (en) 2009-09-30 2011-04-07 Novozymes A/S Steamed bread preparation methods and steamed bread improving compositions
CN102933227A (zh) 2009-12-22 2013-02-13 诺维信公司 包含增强性多肽和淀粉降解酶的组合物及其用途
BR112012018422A2 (pt) 2010-01-29 2015-09-15 Novozymes As processo para produção de biogás com pré-tratamento enzimático.
WO2011100161A1 (en) 2010-02-09 2011-08-18 Novozymes North America, Inc. Addition of alpha - glucosidase and cobalt for producing fermentation products from starch
EP3070171B1 (en) 2010-03-30 2018-06-13 Novozymes A/S Process for enhancing by-products from fermentation processes
WO2011127802A1 (en) 2010-04-14 2011-10-20 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
AU2011263706B2 (en) 2010-06-11 2014-07-24 Novozymes A/S Enzymatic flour correction
US9308064B2 (en) 2010-07-26 2016-04-12 Johnson & Johnson Consumer Inc. Devices and methods for collecting and analyzing fluid samples from the oral cavity
CA2807312A1 (en) 2010-08-02 2012-02-09 Novozymes North America, Inc. Process of producing a fermentation product
CA2817224A1 (en) 2010-11-19 2012-05-24 Novozymes North America, Inc. Processes of producing a fermentation product
US9816112B2 (en) 2010-12-22 2017-11-14 Novozymes A/S Processes for producing fermentation products
CN103339260A (zh) 2011-01-04 2013-10-02 诺维信公司 从含果胶和木素纤维素的材料产生生物气的方法
US9677094B2 (en) 2011-02-07 2017-06-13 Novozymes A/S Process of producing a fermentation product
BR112013032543A2 (pt) 2011-06-28 2017-01-17 Novozymes As processo de produção de biogás
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
CA2840962C (en) 2011-07-06 2021-04-13 Novozymes A/S Alpha amylase variants and polynucleotides encoding same
EP2734633B1 (en) 2011-07-22 2019-05-01 Novozymes North America, Inc. Processes for pretreating cellulosic material and improving hydrolysis thereof
US9128079B2 (en) 2011-08-08 2015-09-08 The Coca-Cola Company Methods of using lung or bronchial epithelial cells to identify bitter taste modulators
CA2861678C (en) 2011-10-11 2021-02-16 Novozymes North America, Inc. Processes for producing fermentation products
IN2014CN04905A (https=) 2011-12-02 2015-09-18 Novozymes As
WO2013083801A2 (en) 2011-12-09 2013-06-13 Novozymes A/S Biogas from substrates comprising animal manure and enzymes
EP2794899A1 (en) 2011-12-21 2014-10-29 Novozymes, Inc. Methods for determining the degradation of a biomass material
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
US9856498B2 (en) 2012-03-30 2018-01-02 Novozymes A/S Processes of producing fermentation products
MX2015001969A (es) 2012-08-17 2015-05-15 Novozymes As Variantes de asparaginasa termoestables y polinucleotidos que las codifican.
WO2014039773A1 (en) * 2012-09-07 2014-03-13 Novozymes A/S Glucoamylase variants and polynucleotides encoding same and uses thereof
CN104870631B (zh) * 2012-12-11 2025-03-25 丹尼斯科美国公司 表达来自烟曲霉的葡糖淀粉酶的里氏木霉宿主细胞和其使用方法
EP3022300B1 (en) 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN105934518A (zh) 2013-09-11 2016-09-07 诺维信公司 用于生产发酵产品的方法
EP3097192B1 (en) 2014-01-22 2018-07-25 Novozymes A/S Pullulanase variants and polynucleotides encoding same
WO2015118123A1 (en) 2014-02-07 2015-08-13 Novozymes A/S Compositions for producing glucose syrups
WO2015143144A1 (en) 2014-03-19 2015-09-24 Novozymes A/S Method for enhancing activity of an x143 polypeptide
EP3129478B1 (en) 2014-04-10 2019-03-27 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN106659168A (zh) 2014-07-08 2017-05-10 卡拉万成分股份有限公司 产生糖和结构改善的焙烤方法和由该方法形成的产品
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
BR112017011275A2 (pt) 2014-12-01 2018-04-03 Novozymes A/S composição, e, processo para a produção de xarope de glicose.
CA2981342A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US10597645B2 (en) 2015-12-22 2020-03-24 Novozymes A/S Process of extracting oil from thin stillage
US10067091B2 (en) 2016-07-29 2018-09-04 Saudi Arabian Oil Company Integrated sediment and water analysis device and method
CN110088287A (zh) 2016-10-17 2019-08-02 诺维信公司 在乙醇发酵过程中减少泡沫的方法
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
BR112019021223A2 (pt) * 2017-04-11 2020-04-28 Novozymes A/S variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
JP7325335B2 (ja) * 2017-05-05 2023-08-14 シーレクター ゲーエムベーハー グルコースイソメラーゼ
CN110730824A (zh) 2017-06-02 2020-01-24 诺维信公司 用于乙醇生产的改善的酵母
US11584783B2 (en) 2017-06-28 2023-02-21 Novozymes A/S Processes for producing a fermentation product
US11220679B2 (en) 2017-08-08 2022-01-11 Novozymes A/S Polypeptides having trehalase activity
CA3070730A1 (en) 2017-09-15 2019-03-21 Novozymes A/S Enzyme blends and processes for improving the nutritional quality of animal feed
CA3075907A1 (en) 2017-10-23 2019-05-02 Novozymes A/S Processes for reducing lactic acid in a biofuel fermentation system
EP3746545A1 (en) 2018-01-29 2020-12-09 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
AU2019222480B9 (en) 2018-02-15 2024-11-28 Microbiogen Pty. Ltd. Improved yeast for ethanol production
WO2019231944A2 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
US20210269833A1 (en) 2018-07-11 2021-09-02 Novozymes A/S Processes for producing fermentation products
MX2021000893A (es) 2018-07-25 2021-03-31 Novozymes As Levadura que expresa enzimas para la producción de etanol.
CN113286889A (zh) 2018-10-08 2021-08-20 诺维信公司 用于生产乙醇的表达酶的酵母
US11622751B2 (en) 2018-12-19 2023-04-11 Johnson & Johnson Consumer Inc. Devices and methods for collecting saliva samples from the oral cavity
BR112021014873A2 (pt) 2019-01-31 2021-10-05 Novozymes A/S Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação
BR112021017085A2 (pt) 2019-04-02 2022-02-08 Novozymes As Processo de produção de um produto de fermentação
BR112022000351A2 (pt) 2019-07-09 2022-05-10 Dupont Nutrition Biosci Aps Composições de enzima particuladas revestidas de gordura
WO2021021458A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
WO2021026201A1 (en) 2019-08-05 2021-02-11 Novozymes A/S Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
MX2021015818A (es) 2019-08-06 2022-02-03 Novozymes As Proteinas de fusion para mejorar la expresion de enzimas.
WO2021046073A1 (en) 2019-09-05 2021-03-11 Dupont Nutrition Biosciences Aps Feed composition
EP4031661A1 (en) 2019-09-16 2022-07-27 Novozymes A/S Polypeptides having beta-glucanase activity and polynucleotides encoding same
CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
PY2084892A (es) 2019-12-16 2022-07-26 Novozymes As Proceso para producir productos de fermentación
EP4103709A2 (en) 2020-02-10 2022-12-21 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
CN116438309A (zh) 2020-11-02 2023-07-14 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
AU2022288057A1 (en) 2021-06-07 2023-12-21 Novozymes A/S Engineered microorganism for improved ethanol fermentation
JP2024536931A (ja) 2021-09-27 2024-10-08 インターナショナル エヌ アンド エイチ デンマーク エーピーエス 飼料添加組成物及びそれを使用する方法
US20250236858A1 (en) * 2021-10-15 2025-07-24 Danisco Us Inc. Glucoamylase variants and methods for use thereof
CN119546193A (zh) 2022-05-17 2025-02-28 国际N&H丹麦有限公司 包含酶组合的饲料添加剂
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
EP4638724A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
JP2025541374A (ja) 2022-12-19 2025-12-18 ノボザイムス アクティーゼルスカブ アラビノフラノシダーゼ及びキシラナーゼを含む組成物、並びにヘミセルロース系繊維の可溶化を増加させるためのその使用
WO2024227153A1 (en) 2023-04-28 2024-10-31 International N&H Denmark Aps Ruminant feed additive compositions
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2026008449A2 (en) 2024-07-04 2026-01-08 Novozymes A/S A process for producing a fermentation product and a concentrated protein co-product

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4272326A (en) 1980-01-24 1981-06-09 Allied Chemical Corporation Enhanced distillation of cyclohexanol from phenol with additional cyclohexanone feed
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JPS62272987A (ja) 1986-05-22 1987-11-27 Hitachi Plant Eng & Constr Co Ltd ブドウ糖の製造方法
DK463887D0 (da) 1987-09-07 1987-09-07 Novo Industri As Gaerleader
JPH01191693A (ja) 1988-01-25 1989-08-01 Tdk Corp デンプンの連続的糖化方法
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
JP2733330B2 (ja) 1989-08-31 1998-03-30 日本食品化工株式会社 高純度澱粉糖の製造方法
CA2038485A1 (en) 1990-03-23 1991-09-24 Donald K. Hadden Nanofiltration process for making dextrose
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
US5162210A (en) * 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
US5231017A (en) 1991-05-17 1993-07-27 Solvay Enzymes, Inc. Process for producing ethanol
US5665585A (en) * 1992-09-03 1997-09-09 Alko-Yhiot Oy Recombinant production of glucoamylase P in trichoderma
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4422198C2 (de) 1994-06-24 1997-08-28 Audi Ag Verfahren zum Steuern der elektrischen Beheizung eines Katalysators
JP4263241B2 (ja) 1996-03-27 2009-05-13 ノボザイムス アクティーゼルスカブ アルカリプロテアーゼを欠失した糸状真菌
WO1998003639A1 (en) * 1996-07-24 1998-01-29 Iowa State University Research Foundation, Inc. PROTEIN ENGINEERING OF GLUCOAMYLASE TO INCREASE pH OPTIMUM, SUBSTRATE SPECIFICITY AND THERMOSTABILITY
US6255084B1 (en) * 1997-11-26 2001-07-03 Novozymes A/S Thermostable glucoamylase
CN1261567C (zh) 1997-11-26 2006-06-28 诺维信公司 热稳定的葡糖淀粉酶
US6352851B1 (en) * 1998-07-15 2002-03-05 Novozymes A/S Glucoamylase variants
WO2000034452A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
US6329186B1 (en) * 1998-12-07 2001-12-11 Novozymes A/S Glucoamylases with N-terminal extensions
CN100510067C (zh) * 1999-07-09 2009-07-08 诺维信公司 葡糖淀粉酶变体
EP1280817A2 (en) * 2000-04-28 2003-02-05 Novozymes A/S Production and use of protein variants having modified immunogenecity
EP1687419B1 (en) * 2003-10-28 2010-02-03 Novozymes North America, Inc. Hybrid enzymes
US7464662B2 (en) * 2004-01-28 2008-12-16 Tokyo Electron Limited Compact, distributed inductive element for large scale inductively-coupled plasma sources

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JP2003504046A (ja) 2003-02-04
AU5805700A (en) 2001-01-30
AR029759A1 (es) 2003-07-16
EP1200566A2 (en) 2002-05-02
US8722369B2 (en) 2014-05-13
AR069653A2 (es) 2010-02-10
CN100510067C (zh) 2009-07-08
WO2001004273A3 (en) 2001-09-07
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US20080213843A1 (en) 2008-09-04
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EP2009098A1 (en) 2008-12-31
US20120208243A1 (en) 2012-08-16

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