CA1321364C - Phosphinothricin-resistance gene active in plants, and its use - Google Patents
Phosphinothricin-resistance gene active in plants, and its useInfo
- Publication number
- CA1321364C CA1321364C CA000556972A CA556972A CA1321364C CA 1321364 C CA1321364 C CA 1321364C CA 000556972 A CA000556972 A CA 000556972A CA 556972 A CA556972 A CA 556972A CA 1321364 C CA1321364 C CA 1321364C
- Authority
- CA
- Canada
- Prior art keywords
- gene
- plants
- vector
- ptc
- resistance gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 57
- 239000012634 fragment Substances 0.000 claims description 31
- 239000013598 vector Substances 0.000 claims description 20
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 230000033228 biological regulation Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000002773 nucleotide Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 108700010070 Codon Usage Proteins 0.000 claims description 3
- 150000001413 amino acids Chemical group 0.000 claims description 3
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 claims 3
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 claims 3
- 230000001131 transforming effect Effects 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 description 31
- 239000013612 plasmid Substances 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 12
- 229920001817 Agar Polymers 0.000 description 8
- 239000008272 agar Substances 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 108020004705 Codon Proteins 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000010348 incorporation Methods 0.000 description 4
- 229930027917 kanamycin Natural products 0.000 description 4
- 229960000318 kanamycin Drugs 0.000 description 4
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 4
- 229930182823 kanamycin A Natural products 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 108020002494 acetyltransferase Proteins 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 229960002337 magnesium chloride Drugs 0.000 description 3
- 235000011147 magnesium chloride Nutrition 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 230000009261 transgenic effect Effects 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000701489 Cauliflower mosaic virus Species 0.000 description 2
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 108020005089 Plant RNA Proteins 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 241001655322 Streptomycetales Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
- 238000006640 acetylation reaction Methods 0.000 description 2
- 102000005421 acetyltransferase Human genes 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229960004261 cefotaxime Drugs 0.000 description 2
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000021615 conjugation Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000005289 controlled pore glass Substances 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- SRVJKTDHMYAMHA-WUXMJOGZSA-N thioacetazone Chemical compound CC(=O)NC1=CC=C(\C=N\NC(N)=S)C=C1 SRVJKTDHMYAMHA-WUXMJOGZSA-N 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 description 1
- 101150034533 ATIC gene Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- XUKUURHRXDUEBC-KAYWLYCHSA-N Atorvastatin Chemical compound C=1C=CC=CC=1C1=C(C=2C=CC(F)=CC=2)N(CC[C@@H](O)C[C@@H](O)CC(O)=O)C(C(C)C)=C1C(=O)NC1=CC=CC=C1 XUKUURHRXDUEBC-KAYWLYCHSA-N 0.000 description 1
- 241000859095 Bero Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000511343 Chondrostoma nasus Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108091033380 Coding strand Proteins 0.000 description 1
- 108091027551 Cointegrate Proteins 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 102100034866 Kallikrein-6 Human genes 0.000 description 1
- 208000034693 Laceration Diseases 0.000 description 1
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 240000008881 Oenanthe javanica Species 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 108091081548 Palindromic sequence Proteins 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- CIUQDSCDWFSTQR-UHFFFAOYSA-N [C]1=CC=CC=C1 Chemical class [C]1=CC=CC=C1 CIUQDSCDWFSTQR-UHFFFAOYSA-N 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 125000004103 aminoalkyl group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 208000037516 chromosome inversion disease Diseases 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229960003276 erythromycin Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
- 108010052968 leupeptin Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 1
- 229960001225 rifampicin Drugs 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3701624 | 1987-01-21 | ||
| DEP3701624.5 | 1987-01-21 | ||
| DEP3737918.6 | 1987-11-07 | ||
| DE19873737918 DE3737918A1 (de) | 1986-08-23 | 1987-11-07 | In pflanzen wirksames resistenzgen gegen phosphinothricin und seine verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1321364C true CA1321364C (en) | 1993-08-17 |
Family
ID=25851724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000556972A Expired - Lifetime CA1321364C (en) | 1987-01-21 | 1988-01-20 | Phosphinothricin-resistance gene active in plants, and its use |
Country Status (13)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8912124B2 (en) | 2010-04-09 | 2014-12-16 | Bayer Cropscience Ag | Use of derivatives of (1-cyanocyclopropyl)phenylphosphinic acid, esters thereof and/or salts thereof for enhancing tolerance in plants to abiotic stress |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3716309A1 (de) * | 1987-05-15 | 1988-11-24 | Hoechst Ag | Resistenzgen gegen phosphinothricin |
| DE3732972A1 (de) * | 1987-07-02 | 1989-01-12 | Hoechst Ag | Resistenzgen gegen phosphinothricin und seine verwendung |
| JP3364616B2 (ja) * | 1989-02-24 | 2003-01-08 | モンサント テクノロジー エルエルシー | 合成植物遺伝子と調製方法 |
| US20010003849A1 (en) | 1989-08-07 | 2001-06-14 | Kenneth A. Barton | Expression of genes in plants |
| US5550318A (en) * | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| WO1991010725A1 (en) | 1990-01-22 | 1991-07-25 | Dekalb Plant Genetics | Fertile transgenic corn plants |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| EP0511979B1 (en) * | 1990-01-26 | 1994-08-10 | Hoechst Schering AgrEvo GmbH | Transgenic plants expressing a prokaryotic ammonium dependent asparagine synthetase |
| DE4003045A1 (de) * | 1990-02-02 | 1991-08-08 | Hoechst Ag | Virus/herbizidresistenz-gene, verfahren zu ihrer herstellung und ihre verwendung |
| US5739082A (en) * | 1990-02-02 | 1998-04-14 | Hoechst Schering Agrevo Gmbh | Method of improving the yield of herbicide-resistant crop plants |
| DE4013099A1 (de) * | 1990-04-25 | 1991-10-31 | Hoechst Ag | Verfahren zur transformation somatischer embryonen von nutzpflanzen |
| US5792936A (en) * | 1990-06-23 | 1998-08-11 | Hoechst Aktiengesellschaft | Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation |
| US5767367A (en) | 1990-06-23 | 1998-06-16 | Hoechst Aktiengesellschaft | Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation |
| IL101119A0 (en) * | 1992-03-03 | 1992-11-15 | Univ Ramot | Transgenic wheat |
| DE4222407C1 (de) * | 1992-07-08 | 1993-10-07 | Max Planck Gesellschaft | Modulartiges Promotor-Konstrukt |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| IL122270A0 (en) * | 1997-11-20 | 1998-04-05 | Yeda Res & Dev | DNA molecules conferring to plants resistance to a herbicide and plants transformed thereby |
| DE19836700A1 (de) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Herbizide Mittel für tolerante oder resistente Getreidekulturen |
| PL215315B1 (pl) * | 1998-08-13 | 2013-11-29 | Bayer Cropscience Ag | Zastosowanie kompozycji zawierajacej glifosat i diflufenzopir, sposób zwalczania szkodliwych roslin w uprawach kukurydzy oraz kompozycja herbicydów |
| DE19836659A1 (de) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Herbizide Mittel für tolerante oder resistente Baumwollkulturen |
| DE19836684A1 (de) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Herbizide Mittel für tolerante oder resistente Reiskulturen |
| DE19836660A1 (de) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Herbizide Mittel für tolerante oder resistente Sojakulturen |
| US6333449B1 (en) | 1998-11-03 | 2001-12-25 | Plant Genetic Systems, N.V. | Glufosinate tolerant rice |
| US6395485B1 (en) | 2000-01-11 | 2002-05-28 | Aventis Cropscience N.V. | Methods and kits for identifying elite event GAT-ZM1 in biological samples |
| US7517975B2 (en) | 2000-09-26 | 2009-04-14 | Pioneer Hi-Bred International, Inc. | Nucleotide sequences mediating male fertility and method of using same |
| ATE510015T1 (de) | 2001-02-08 | 2011-06-15 | Hexima Ltd | Aus pflanzen stammende moleküle und diese codierende genetische sequenzen sowie verwendungen dafür |
| EP1279737A1 (en) | 2001-07-27 | 2003-01-29 | Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. | Transformation method for obtaining marker-free plants |
| CA2517675A1 (en) * | 2003-03-26 | 2004-10-07 | Cytos Biotechnology Ag | Packaging of immunostimulatory oligonucleotides into virus-like particles: method of preparation and use |
| AU2003902253A0 (en) | 2003-05-12 | 2003-05-29 | The University Of Queensland | Method for increasing product yield |
| CA2586241C (en) | 2004-11-17 | 2013-07-30 | Hokko Chemical Industry Co., Ltd. | Herbicide-resistance gene and utilization thereof |
| US8993846B2 (en) | 2005-09-06 | 2015-03-31 | Monsanto Technology Llc | Vectors and methods for improved plant transformation efficiency |
| CA2625031C (en) | 2005-10-13 | 2016-07-19 | Monsanto Technology Llc | Methods for producing hybrid seed |
| JP2009537150A (ja) | 2006-05-16 | 2009-10-29 | モンサント テクノロジー エルエルシー | 植物形質転換用の非−アグロバクテリウム細菌種の使用 |
| US7939721B2 (en) | 2006-10-25 | 2011-05-10 | Monsanto Technology Llc | Cropping systems for managing weeds |
| EP1949785A1 (en) | 2007-01-26 | 2008-07-30 | Coöperatie AVEBE U.A. | Use of R-genes as a selection marker in plant transformation and use of cisgenes in plant transformation |
| EP2450448B1 (en) | 2007-03-09 | 2014-09-17 | Monsanto Technology LLC | Methods for plant transformation using spectinomycin selection |
| EP2173883A1 (en) | 2007-08-03 | 2010-04-14 | Pioneer Hi-Bred International Inc. | Msca1 nucleotide sequences impacting plant male fertility and method of using same |
| MX2010005937A (es) | 2007-11-27 | 2010-06-15 | Commw Scient Ind Res Org | Plantas con metabolismo de almidon modificado. |
| US8338665B2 (en) | 2008-07-16 | 2012-12-25 | Monsanto Technology Llc | Methods and vectors for producing transgenic plants |
| CN102202498B (zh) | 2008-07-21 | 2016-09-07 | 澳大利亚联邦科学与工业研究组织 | 改良的棉籽油及应用 |
| BRPI0921467B1 (pt) | 2008-11-18 | 2021-12-14 | Commonwealth Scientific And Industrial Research Organisation | Processo para a produção de óleo compreendendo ácido eicosapentaenóico (epa), ácido docosapentaenóico (dpa) e ácido docosahexaenóico (dha) |
| UA110021C2 (uk) | 2009-09-18 | 2015-11-10 | Спосіб забезпечення принаймні часткової резистентності чи підвищення резистентності у рослини проти ооміцетної інфекції | |
| CN103025166A (zh) | 2010-05-21 | 2013-04-03 | 拜耳知识产权有限责任公司 | 用于耐受性或抗性谷物作物的除草剂 |
| CA2799690A1 (en) | 2010-05-21 | 2011-11-24 | Bayer Intellectual Property Gmbh | Herbicidal agents for tolerant or resistant rape cultures |
| US20110287934A1 (en) | 2010-05-21 | 2011-11-24 | Bayer Cropscience Ag | Herbicidal composition for tolerant or resistant rice crops |
| CN103025168A (zh) | 2010-05-21 | 2013-04-03 | 拜耳知识产权有限责任公司 | 用于耐受性或抗性玉米栽培种的除草剂 |
| EP2390332A1 (en) | 2010-05-31 | 2011-11-30 | Coöperatie Avebe U.A. | Cloning and exploitation of a functional R-gene from Solanum x edinense |
| AR083323A1 (es) | 2010-06-28 | 2013-02-21 | Commw Scient Ind Res Org | Metodos para producir lipidos |
| RU2611188C2 (ru) | 2010-07-29 | 2017-02-21 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Штаммы agrobacterium, модифицированные для увеличения частоты трансформации растений |
| NL2006378C2 (en) | 2011-03-11 | 2012-09-12 | Univ Wageningen | Tyclv resistance. |
| EP2524602A1 (de) | 2011-05-20 | 2012-11-21 | Bayer CropScience AG | Herbizide Mittel für tolerante oder resistente Sojakulturen |
| AU2012327162A1 (en) | 2011-12-27 | 2013-07-11 | Commonwealth Scientific And Industrial Research Organisation | Production of dihydrosterculic acid and derivatives thereof |
| AU2012324003B2 (en) | 2011-12-27 | 2016-05-26 | Commonwealth Scientific And Industrial Research Organisation | Simultaneous gene silencing and supressing gene silencing in the same cell |
| MX2014007964A (es) | 2011-12-27 | 2014-10-06 | Commw Scient Ind Res Org | Procesos para producir lipidos. |
| WO2013176548A1 (en) | 2012-05-25 | 2013-11-28 | Wageningen Universiteit | New plant resistance gene |
| MY187101A (en) | 2012-06-15 | 2021-08-31 | Commw Scient Ind Res Org | Production of long chain polyunsaturated fatty acids in plant cells |
| UA117816C2 (uk) | 2012-11-06 | 2018-10-10 | Байєр Кропсайєнс Акцієнгезелльшафт | Гербіцидна комбінація для толерантних соєвих культур |
| WO2014112875A1 (en) | 2013-01-17 | 2014-07-24 | Wageningen Universiteit | A new method to provide resistance to bacterial soft rot in plants |
| AU2014308558B2 (en) | 2013-08-21 | 2020-11-12 | Commonwealth Scientific And Industrial Research Organisation | Rust resistance gene |
| KR102386838B1 (ko) | 2013-12-18 | 2022-04-15 | 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 | 장쇄 다중불포화 지방산을 포함하는 지질 |
| SG11201610596PA (en) | 2014-06-27 | 2017-01-27 | Commw Scient Ind Res Org | Lipid comprising docosapentaenoic acid |
| PH12016502588B1 (en) | 2014-07-07 | 2024-01-17 | Commw Scient Ind Res Org | Processes for producing industrial products from plant lipids |
| CN104721835A (zh) * | 2014-11-26 | 2015-06-24 | 浙江医药高等专科学校 | 抗黑色素瘤dna质粒疫苗及其制备方法 |
| NL2014107B1 (en) | 2015-01-09 | 2016-09-29 | Limgroup B V | New methods and products for breeding of asparagus. |
| US11236347B2 (en) | 2015-08-28 | 2022-02-01 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| US11732269B2 (en) | 2015-10-02 | 2023-08-22 | Monsanto Technology Llc | Recombinant maize B chromosome sequence and uses thereof |
| AU2016355920B2 (en) | 2015-11-18 | 2020-11-12 | Commonwealth Scientific And Industrial Research Organisation | Rice grain with thickened aleurone |
| US11732268B2 (en) | 2016-06-28 | 2023-08-22 | Monsanto Technology Llc | Methods and compositions for use in genome modification in plants |
| EP3935169A4 (en) | 2019-03-08 | 2022-12-21 | Commonwealth Scientific and Industrial Research Organisation | EXPRESSION OF NITROGENASE POLYPEPTIDES IN PLANT CELLS |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3765449D1 (de) * | 1986-03-11 | 1990-11-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| EP0257542B1 (de) * | 1986-08-23 | 1992-05-06 | Hoechst Aktiengesellschaft | Resistenzgen gegen Phosphinothricin und seine Verwendung |
| DE3716309A1 (de) * | 1987-05-15 | 1988-11-24 | Hoechst Ag | Resistenzgen gegen phosphinothricin |
| DE3732972A1 (de) * | 1987-07-02 | 1989-01-12 | Hoechst Ag | Resistenzgen gegen phosphinothricin und seine verwendung |
-
1987
- 1987-02-10 CN CN198787100603A patent/CN87100603A/zh active Pending
-
1988
- 1988-01-19 NZ NZ223227A patent/NZ223227A/xx unknown
- 1988-01-19 EP EP88100631A patent/EP0275957B1/de not_active Expired - Lifetime
- 1988-01-19 FI FI880216A patent/FI116067B/fi not_active IP Right Cessation
- 1988-01-19 ES ES88100631T patent/ES2054708T3/es not_active Expired - Lifetime
- 1988-01-19 DE DE8888100631T patent/DE3878699D1/de not_active Expired - Lifetime
- 1988-01-20 AU AU10619/88A patent/AU609082B2/en not_active Expired
- 1988-01-20 IL IL85143A patent/IL85143A0/xx active IP Right Grant
- 1988-01-20 HU HU88217A patent/HU215079B/hu unknown
- 1988-01-20 CA CA000556972A patent/CA1321364C/en not_active Expired - Lifetime
- 1988-01-20 CN CN88100322A patent/CN1057120C/zh not_active Expired - Lifetime
- 1988-01-20 DK DK198800239A patent/DK175800B1/da not_active IP Right Cessation
- 1988-01-21 JP JP63011851A patent/JP2993964B2/ja not_active Expired - Lifetime
-
1993
- 1993-05-14 GR GR930400667T patent/GR3007859T3/el unknown
-
1997
- 1997-07-22 JP JP9196218A patent/JP3062125B2/ja not_active Expired - Lifetime
- 1997-07-22 JP JP09196219A patent/JP3093686B2/ja not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8912124B2 (en) | 2010-04-09 | 2014-12-16 | Bayer Cropscience Ag | Use of derivatives of (1-cyanocyclopropyl)phenylphosphinic acid, esters thereof and/or salts thereof for enhancing tolerance in plants to abiotic stress |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0275957B1 (de) | 1993-03-03 |
| IL85143A0 (en) | 1988-06-30 |
| FI116067B (fi) | 2005-09-15 |
| CN88100322A (zh) | 1988-08-10 |
| ES2054708T3 (es) | 1994-08-16 |
| JPH1080278A (ja) | 1998-03-31 |
| FI880216A0 (fi) | 1988-01-19 |
| DK23988D0 (da) | 1988-01-20 |
| FI880216L (fi) | 1988-07-22 |
| EP0275957A3 (en) | 1990-02-28 |
| DK23988A (da) | 1988-07-22 |
| AU609082B2 (en) | 1991-04-26 |
| EP0275957A2 (de) | 1988-07-27 |
| AU1061988A (en) | 1988-07-28 |
| HU215079B (hu) | 1998-09-28 |
| JP3062125B2 (ja) | 2000-07-10 |
| DE3878699D1 (de) | 1993-04-08 |
| JPS63273479A (ja) | 1988-11-10 |
| JP3093686B2 (ja) | 2000-10-03 |
| JP2993964B2 (ja) | 1999-12-27 |
| HUT47636A (en) | 1989-03-28 |
| DK175800B1 (da) | 2005-02-28 |
| JPH1080289A (ja) | 1998-03-31 |
| GR3007859T3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1993-08-31 |
| CN87100603A (zh) | 1988-08-10 |
| NZ223227A (en) | 1989-06-28 |
| CN1057120C (zh) | 2000-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1321364C (en) | Phosphinothricin-resistance gene active in plants, and its use | |
| US5276268A (en) | Phosphinothricin-resistance gene, and its use | |
| US6100446A (en) | Microorganisms and plasmids for 2,4-dichlorophenoxyacetic acid (2,4-D)monooxygenase formation and process for the production of these plasmids and strains | |
| De Bruijn et al. | Rhizobium meliloti 1021 has three differentially regulated loci involved in glutamine biosynthesis, none of which is essential for symbiotic nitrogen fixation | |
| US5595889A (en) | Process for integration of a chosen gene on the chromosome of a bacterium using Mu transposons | |
| EP0242246B1 (en) | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering | |
| Deutch et al. | Analysis of the Escherichia coli proBA locus by DNA and protein sequencing | |
| JP2749424B2 (ja) | ホスフィノトリシン耐性遺伝子の使用 | |
| JPH028714B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| EP0205518A1 (en) | Process for preparing genetically stably transformed monocotyledonous plant cells | |
| EP0140556B1 (en) | A t-dna derived vector | |
| ES2308788T3 (es) | Proceso para la produccion de acido (s)- o (r)-3,3,3-trifluoro-2-hidroxi-2-metilpropionico. | |
| US6187996B1 (en) | Plant promoter comprising a G-box element, GCCACGTGCC or GCCACGTGAG, and an application thereof | |
| Hooykaas et al. | Agrobacterium tumor inducing plasmids: potential vectors for the genetic engineering of plants | |
| JPS62155081A (ja) | 新規微生物およびそれを用いる醗酵法によるビオチンの製造法 | |
| Van Montagu et al. | The interaction of Agrobacterium Ti-plasmid DNA and plant cells | |
| IL103811A (en) | Dna sequences coding for betaine utilization, vectors containing the same, microorganisms transformed therewith and uses thereof | |
| US7049097B2 (en) | Antibiotics-independent vector for constant high-expression and method for gene expression using the same | |
| KR102433234B1 (ko) | L-시트룰린 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-시트룰린의 생산 방법 | |
| US6197590B1 (en) | Process for integration of a chosen gene on the chromosome of a bacterium obtained by the said process | |
| JP2000333677A (ja) | 植物の青枯病防除方法 | |
| IL85143A (en) | Resistance gene against phosphinothricin | |
| CN116751759A (zh) | 红果酸合成酶及其相关生物材料和应用 | |
| López-Lara et al. | Nodulation Efficiency nfe Gene Homolog of Agrobacterium Ornithine Cyclodeaminase | |
| HK1000519B (en) | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |
Effective date: 20100817 |