CA1309932C - Nucleic acid hybridization technique and kit therefor - Google Patents

Nucleic acid hybridization technique and kit therefor

Info

Publication number
CA1309932C
CA1309932C CA000548798A CA548798A CA1309932C CA 1309932 C CA1309932 C CA 1309932C CA 000548798 A CA000548798 A CA 000548798A CA 548798 A CA548798 A CA 548798A CA 1309932 C CA1309932 C CA 1309932C
Authority
CA
Canada
Prior art keywords
probe
probes
polynucleotide
solid carrier
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA000548798A
Other languages
English (en)
French (fr)
Inventor
Edward Gray Rice
Joan Marlyn Beebe
Jeffry Joseph Leary
Linda Lee Glanville
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Application granted granted Critical
Publication of CA1309932C publication Critical patent/CA1309932C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA000548798A 1986-10-14 1987-10-07 Nucleic acid hybridization technique and kit therefor Expired - Lifetime CA1309932C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91920186A 1986-10-14 1986-10-14
US919,201 1986-10-14

Publications (1)

Publication Number Publication Date
CA1309932C true CA1309932C (en) 1992-11-10

Family

ID=25441695

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000548798A Expired - Lifetime CA1309932C (en) 1986-10-14 1987-10-07 Nucleic acid hybridization technique and kit therefor

Country Status (7)

Country Link
JP (1) JPH01501339A (no)
AU (1) AU8103687A (no)
CA (1) CA1309932C (no)
DK (1) DK324088D0 (no)
FI (1) FI882800A0 (no)
NO (1) NO882593D0 (no)
WO (1) WO1988002785A2 (no)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060814B2 (en) 2000-03-31 2006-06-13 Sanko Junyaku Co., Ltd. Probe for constructing probe-polymer method of constructing probe-polymer and utilization thereof

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU593151B2 (en) * 1986-11-12 1990-02-01 Molecular Diagnostics, Inc. Method for the detection of nucleic acid hybrids
DE3888653T2 (de) * 1987-12-21 1994-07-07 Applied Biosystems Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz.
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
DE68928853T2 (de) * 1988-05-20 1999-08-05 Cetus Corp Befestigung von sequenzspezifischen proben
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
ATE478886T1 (de) 2003-07-25 2010-09-15 Idenix Pharmaceuticals Inc Purin nucleoside für die behandlung von durch flavividrae verursachten krankheiten, einschliesslich hepatitis c
WO2005071401A2 (en) * 2004-01-15 2005-08-04 Chiron Corporation Homogeneous multiplex assay for nucleic acid targets
CN103733047B (zh) 2011-08-11 2016-03-30 奥林巴斯株式会社 目标粒子的检测方法
WO2013031365A1 (ja) 2011-08-30 2013-03-07 オリンパス株式会社 標的粒子の検出方法
CN104115003B (zh) 2012-02-22 2016-03-23 奥林巴斯株式会社 目标粒子的检测方法
JP6095645B2 (ja) 2012-03-21 2017-03-15 オリンパス株式会社 標的核酸分子の検出方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2953610C1 (de) * 1979-03-19 1985-04-11 International Diagnostic Technology, Inc., Santa Clara, Calif. Verfahren zum Bestimmen eines immunologisch aktiven Stoffs
US4487830A (en) * 1982-05-14 1984-12-11 American Hoechst Corporation Enzyme/immunofluorescent assay for autoantibodies
CA1222680A (en) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Testing dna samples for particular nucleotide sequences
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
GB8432118D0 (en) * 1984-12-19 1985-01-30 Malcolm A D B Sandwich hybridisation technique
CA1272443A (en) * 1985-02-22 1990-08-07 Nanibhushan Dattagupta Solution-phase dual hybridization assay for detecting polynucleotide sequences
GB8508431D0 (en) * 1985-04-01 1985-05-09 English Clays Lovering Pochin Paper coating apparatus
GB8509367D0 (en) * 1985-04-12 1985-05-15 Amersham Int Plc Nucleic acid hybridisation
ES8707343A1 (es) * 1985-06-13 1987-07-16 Amgen Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada
AU7015287A (en) * 1986-03-19 1987-09-24 Cetus Corporation Detection of nucleic acid sequence using liquid hybridization method
GB8607101D0 (en) * 1986-03-21 1986-04-30 Serono Diagnostics Ltd Immunoassay

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060814B2 (en) 2000-03-31 2006-06-13 Sanko Junyaku Co., Ltd. Probe for constructing probe-polymer method of constructing probe-polymer and utilization thereof

Also Published As

Publication number Publication date
NO882593L (no) 1988-06-13
FI882800A (fi) 1988-06-13
FI882800A0 (fi) 1988-06-13
WO1988002785A3 (en) 1988-07-14
DK324088D0 (da) 1988-06-14
JPH01501339A (ja) 1989-05-11
AU8103687A (en) 1988-05-06
WO1988002785A2 (en) 1988-04-21
NO882593D0 (no) 1988-06-13

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