BRPI0812538B1 - processo para a síntese de uma proteína de interesse com fucosilação e xilosilação reduzidas, ácido nucleico, e proteína híbrida gnt1-galt - Google Patents

processo para a síntese de uma proteína de interesse com fucosilação e xilosilação reduzidas, ácido nucleico, e proteína híbrida gnt1-galt Download PDF

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Publication number
BRPI0812538B1
BRPI0812538B1 BRPI0812538-4A BRPI0812538A BRPI0812538B1 BR PI0812538 B1 BRPI0812538 B1 BR PI0812538B1 BR PI0812538 A BRPI0812538 A BR PI0812538A BR PI0812538 B1 BRPI0812538 B1 BR PI0812538B1
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BR
Brazil
Prior art keywords
protein
plant
interest
nucleotide sequence
galt
Prior art date
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BRPI0812538-4A
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English (en)
Portuguese (pt)
Inventor
Marc-Andre D'Aoust
Louis-Philippe Vézina
Stephanie Aquin
Estelle Marquet-Blouin
Muriel Bardor
Carole Burel
Loic Faye
Patrice Lerouge
Veronique Gomord
Christophe Rihouey
Thomas Paccalet
Christophe Sourrouille
Original Assignee
Medicago Inc.
Centre National De La Recherche Scientifique
Université de Rouen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Medicago Inc., Centre National De La Recherche Scientifique, Université de Rouen filed Critical Medicago Inc.
Publication of BRPI0812538A2 publication Critical patent/BRPI0812538A2/pt
Publication of BRPI0812538B1 publication Critical patent/BRPI0812538B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cultivation Of Plants (AREA)
BRPI0812538-4A 2007-06-15 2008-06-13 processo para a síntese de uma proteína de interesse com fucosilação e xilosilação reduzidas, ácido nucleico, e proteína híbrida gnt1-galt BRPI0812538B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94434407P 2007-06-15 2007-06-15
US60/944,344 2007-06-15
PCT/CA2008/001139 WO2008151440A1 (en) 2007-06-15 2008-06-13 Modifying glycoprotein production in plants

Publications (2)

Publication Number Publication Date
BRPI0812538A2 BRPI0812538A2 (pt) 2014-09-30
BRPI0812538B1 true BRPI0812538B1 (pt) 2021-03-16

Family

ID=40129187

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0812538-4A BRPI0812538B1 (pt) 2007-06-15 2008-06-13 processo para a síntese de uma proteína de interesse com fucosilação e xilosilação reduzidas, ácido nucleico, e proteína híbrida gnt1-galt

Country Status (24)

Country Link
US (1) US20110008837A1 (https=)
EP (1) EP2155880B1 (https=)
JP (1) JP5783720B2 (https=)
KR (2) KR101385050B1 (https=)
CN (3) CN104962579A (https=)
AU (1) AU2008261527C1 (https=)
BR (1) BRPI0812538B1 (https=)
CA (2) CA2795379A1 (https=)
DK (1) DK2155880T3 (https=)
EG (1) EG26600A (https=)
ES (1) ES2624776T3 (https=)
HU (1) HUE031698T2 (https=)
IL (1) IL202568A (https=)
MA (1) MA31516B1 (https=)
MX (1) MX2009013665A (https=)
NZ (2) NZ598417A (https=)
PL (1) PL2155880T3 (https=)
PT (1) PT2155880T (https=)
RU (2) RU2499053C2 (https=)
SG (1) SG185249A1 (https=)
SI (1) SI2155880T1 (https=)
TN (1) TN2009000512A1 (https=)
WO (1) WO2008151440A1 (https=)
ZA (1) ZA201000207B (https=)

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CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
AU2009267769B2 (en) 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
NZ590351A (en) 2008-07-18 2012-11-30 Medicago Inc HA1 domain totally or partially free of N-linked glycosylation operatively linked to a regulatory region
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
KR101773431B1 (ko) 2009-09-22 2017-09-12 메디카고 인코포레이티드 식물-유래 ｖｌｐ의 제조방법
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
WO2012170678A1 (en) * 2011-06-07 2012-12-13 Fraunhofer Usa, Inc. In vivo de-glycosylation of recombinant proteins by co-expression with pngase f
US9181532B2 (en) 2011-07-29 2015-11-10 Icon Genetics Gmbh Production of galactosylated N-glycans in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
KR20130125337A (ko) * 2012-05-08 2013-11-18 경상대학교산학협력단 고-만노오스형 n-글라이칸을 생산하는 식물체 및 이를 이용한 고-만노오스형 n-글라이칸의 생산방법
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
WO2016079739A2 (en) * 2014-11-20 2016-05-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
US11499160B2 (en) 2016-12-01 2022-11-15 Plantform Corporation Transgenic plant with reduced fucosyltransferase and xylosyltransferase activity
US11649465B2 (en) * 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
WO2022153973A1 (ja) * 2021-01-15 2022-07-21 デンカ株式会社 栽培条件を決定する方法、及び目的タンパク質又は目的ペプチドの製造方法

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DK1137789T3 (da) * 1998-12-09 2010-11-08 Phyton Holdings Llc Fremgangsmåde til fremstilling af et glycoprotein med glycosylering af human type
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
KR100817660B1 (ko) * 1999-10-04 2008-03-27 메디카고 인코포레이티드 외래 유전자의 발현을 조절하는 프로모터
BR0014945A (pt) * 1999-10-21 2004-08-31 Monsanto Co Modificação pós-traducional de proteìnas recombinantes produzidas em plantas
ES2349427T3 (es) * 1999-10-26 2011-01-03 Stichting Dienst Landbouwkundig Onderzoek Glicosilación de tipo mamífero en plantas.
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CN1665934B (zh) * 2002-03-19 2010-05-26 国际植物研究所 GNTIII(UDP-N乙酰葡萄糖胺:β-D-甘露糖苷β(1,4)-N-乙酰葡萄糖胺基-转移酶III)在植物中的表达
CN101613705A (zh) * 2002-03-19 2009-12-30 国际植物研究所 在植物中优化聚糖生成
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EP2248892B1 (en) * 2003-01-22 2015-04-22 Roche Glycart AG Fusion constructs and use of same to produce antibodies with increased FC receptor binding affinity and effector function
KR20060035596A (ko) * 2003-05-05 2006-04-26 보이스 톰슨 인스터튜트 포 플랜트 리서치 외래유전자도입 식물로부터 유래된 면역보호성 조성물을제조하기 위한 벡터와 세포
JP2006212019A (ja) * 2004-04-30 2006-08-17 National Institute Of Agrobiological Sciences 植物を用いたユビキノン−１０の製造方法

Also Published As

Publication number Publication date
WO2008151440A4 (en) 2009-02-26
CA2795379A1 (en) 2008-12-18
MX2009013665A (es) 2010-06-02
AU2008261527B8 (en) 2014-04-24
JP5783720B2 (ja) 2015-09-24
WO2008151440A8 (en) 2010-03-04
HUE031698T2 (en) 2017-07-28
CA2700180A1 (en) 2008-12-18
NZ598417A (en) 2013-06-28
US20110008837A1 (en) 2011-01-13
CN101918559A (zh) 2010-12-15
IL202568A0 (en) 2011-08-01
SG185249A1 (en) 2012-11-29
KR101385050B1 (ko) 2014-05-13
CN103382487A (zh) 2013-11-06
MA31516B1 (fr) 2010-07-01
ZA201000207B (en) 2013-09-25
BRPI0812538A2 (pt) 2014-09-30
AU2008261527A8 (en) 2010-01-21
ES2624776T3 (es) 2017-07-17
PT2155880T (pt) 2016-11-18
EG26600A (en) 2014-03-23
RU2013137208A (ru) 2015-02-20
CN104962579A (zh) 2015-10-07
EP2155880A4 (en) 2010-08-04
SI2155880T1 (sl) 2017-03-31
AU2008261527B2 (en) 2014-04-10
WO2008151440A1 (en) 2008-12-18
IL202568A (en) 2015-02-26
AU2008261527C1 (en) 2014-09-04
DK2155880T3 (en) 2016-12-05
PL2155880T3 (pl) 2017-06-30
EP2155880B1 (en) 2016-08-10
TN2009000512A1 (en) 2011-03-31
NZ581944A (en) 2012-03-30
AU2008261527A1 (en) 2008-12-18
KR20100037602A (ko) 2010-04-09
RU2499053C2 (ru) 2013-11-20
KR20130075787A (ko) 2013-07-05
JP2010531136A (ja) 2010-09-24
EP2155880A1 (en) 2010-02-24
CA2700180C (en) 2013-01-29
RU2010101024A (ru) 2011-07-20

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