BRPI0719923B1 - Métodos para sequenciamento de um ácido nucléico alvo, para conversão de um componente de ocorrência não natural em uma molécula de ácido nucléico em um componente de ocorrência natural, para terminar uma síntese de ácido nucléico, e para realizar o sequenciamento de sanger ou tipo sanger, e compostos - Google Patents

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Publication number

BRPI0719923B1

BRPI0719923B1 BRPI0719923-6A BRPI0719923A BRPI0719923B1 BR PI0719923 B1 BRPI0719923 B1 BR PI0719923B1 BR PI0719923 A BRPI0719923 A BR PI0719923A BR PI0719923 B1 BRPI0719923 B1 BR PI0719923B1

Authority

BR

Brazil

Prior art keywords

mmol

compound

triphosphate

solution

polymerase

Prior art date

2006-12-05

Links

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• 238000012163 sequencing technique Methods 0.000 title claims description 45
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• 239000007850 fluorescent dye Substances 0.000 claims abstract description 23
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• 239000001226 triphosphate Substances 0.000 claims description 125
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• -1 2-nitrophenyl Chemical group 0.000 claims description 100
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims description 95
• 238000006243 chemical reaction Methods 0.000 claims description 84
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• 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 17
• 125000005647 linker group Chemical group 0.000 claims description 12
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• ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 4
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• 125000003456 2,6-dinitrophenyl group Chemical group [H]C1=C([H])C(=C(*)C(=C1[H])[N+]([O-])=O)[N+]([O-])=O 0.000 claims description 3
• GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 claims description 3
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• XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 104
• HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 102
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• VKIGAWAEXPTIOL-UHFFFAOYSA-N 2-hydroxyhexanenitrile Chemical compound CCCCC(O)C#N VKIGAWAEXPTIOL-UHFFFAOYSA-N 0.000 description 79
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