CA2670937C - Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing - Google Patents

Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing Download PDF

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Publication number
CA2670937C
CA2670937C CA2670937A CA2670937A CA2670937C CA 2670937 C CA2670937 C CA 2670937C CA 2670937 A CA2670937 A CA 2670937A CA 2670937 A CA2670937 A CA 2670937A CA 2670937 C CA2670937 C CA 2670937C
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Canada
Prior art keywords
compound
mmol
nucleic acid
triphosphate
incorporation
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CA2670937A
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English (en)
French (fr)
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CA2670937A1 (en
Inventor
Vladislav A. Litosh
Brian P. Stupi
Michael L. Metzker
Weidong Wu
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Agilent Technologies Inc
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LaserGen Inc
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Priority claimed from US11/567,193 external-priority patent/US7893227B2/en
Priority claimed from US11/567,189 external-priority patent/US7897737B2/en
Application filed by LaserGen Inc filed Critical LaserGen Inc
Publication of CA2670937A1 publication Critical patent/CA2670937A1/en
Application granted granted Critical
Publication of CA2670937C publication Critical patent/CA2670937C/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA2670937A 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing Active CA2670937C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11/567,193 US7893227B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US11/567,189 2006-12-05
US11/567,189 US7897737B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US11/567,193 2006-12-05
PCT/US2007/086559 WO2008070749A2 (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Publications (2)

Publication Number Publication Date
CA2670937A1 CA2670937A1 (en) 2008-06-12
CA2670937C true CA2670937C (en) 2016-07-26

Family

ID=39493724

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2670937A Active CA2670937C (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Country Status (12)

Country Link
EP (1) EP2125856B1 (https=)
JP (1) JP5513122B2 (https=)
KR (1) KR101504640B1 (https=)
CN (1) CN102443030B (https=)
AU (1) AU2007329361B2 (https=)
BR (1) BRPI0719923B1 (https=)
CA (1) CA2670937C (https=)
IL (1) IL199051A (https=)
MX (2) MX338089B (https=)
NZ (1) NZ577303A (https=)
RU (1) RU2009121089A (https=)
WO (1) WO2008070749A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
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US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013105025A1 (en) * 2012-01-13 2013-07-18 Koninklijke Philips N.V. Dna sequencing with reagent recycling on wiregrid
CN103602719B (zh) * 2013-04-07 2016-06-08 北京迈基诺基因科技有限责任公司 一种基因测序方法
WO2015015913A1 (ja) * 2013-07-31 2015-02-05 株式会社 日立ハイテクノロジーズ 核酸分析用フローセル、及び核酸分析装置
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
RU2582198C1 (ru) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
CN105001292A (zh) * 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN113272443B (zh) 2019-01-07 2025-01-10 安捷伦科技有限公司 用于单细胞中的基因组dna和基因表达分析的组合物和方法
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
WO2021194640A1 (en) * 2020-03-26 2021-09-30 Agilent Technologies, Inc. Methods and compositions for multistage primer extension reactions
GB2605404A (en) * 2021-03-30 2022-10-05 Sumitomo Chemical Co Sequencing method
WO2022263489A1 (en) * 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
IL309368A (en) * 2021-06-29 2024-02-01 Agilent Technologies Inc polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS
CN114250283B (zh) * 2021-10-15 2022-10-04 深圳铭毅智造科技有限公司 基于环境敏感染料的单色荧光mrt基因测序试剂及方法

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US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
YU187991A (sh) * 1990-12-11 1994-09-09 Hoechst Aktiengesellschaft 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba
US6211165B1 (en) * 1997-05-09 2001-04-03 The Trustees Of The University Of Pennsylvania Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
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AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
DE60212642T2 (de) * 2001-04-10 2007-06-14 ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
GB0128526D0 (en) * 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
PT3438116T (pt) 2002-08-23 2021-03-23 Illumina Cambridge Ltd Nucleótidos identificados
CZ294538B6 (cs) * 2002-12-30 2005-01-12 Ústav Experimentální Botaniky Akademie Vědčeské Re Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující
EP2436778A3 (en) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry

Also Published As

Publication number Publication date
IL199051A (en) 2013-11-28
AU2007329361A1 (en) 2008-06-12
JP5513122B2 (ja) 2014-06-04
RU2009121089A (ru) 2011-01-20
MX338089B (es) 2016-04-01
CN102443030A (zh) 2012-05-09
BRPI0719923A2 (pt) 2014-03-04
EP2125856A2 (en) 2009-12-02
CN102443030B (zh) 2015-12-16
AU2007329361B2 (en) 2013-07-18
CA2670937A1 (en) 2008-06-12
KR101504640B1 (ko) 2015-03-20
BRPI0719923B1 (pt) 2021-08-17
MX2009005936A (es) 2010-01-15
WO2008070749A2 (en) 2008-06-12
JP2011506265A (ja) 2011-03-03
HK1169413A1 (zh) 2013-01-25
EP2125856B1 (en) 2016-09-14
NZ577303A (en) 2011-12-22
WO2008070749A3 (en) 2008-11-27
KR20090101451A (ko) 2009-09-28
EP2125856A4 (en) 2013-12-25

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