CN102443030B - 光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法 - Google Patents
光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法 Download PDFInfo
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- CN102443030B CN102443030B CN201110376726.1A CN201110376726A CN102443030B CN 102443030 B CN102443030 B CN 102443030B CN 201110376726 A CN201110376726 A CN 201110376726A CN 102443030 B CN102443030 B CN 102443030B
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- AFQIYTIJXGTIEY-UHFFFAOYSA-N hydrogen carbonate;triethylazanium Chemical compound OC(O)=O.CCN(CC)CC AFQIYTIJXGTIEY-UHFFFAOYSA-N 0.000 description 27
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Landscapes
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- Biotechnology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
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| US11/567,193 US7893227B2 (en) | 2006-12-05 | 2006-12-05 | 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing |
| US11/567,189 | 2006-12-05 | ||
| US11/567,189 US7897737B2 (en) | 2006-12-05 | 2006-12-05 | 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing |
| US11/567,193 | 2006-12-05 | ||
| CN200780049437.2A CN101636406A (zh) | 2006-12-05 | 2007-12-05 | 光可断裂的标记核苷酸和核苷与标记的核苷酸和核苷以及其在dna测序中的使用方法 |
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| US7897737B2 (en) | 2006-12-05 | 2011-03-01 | Lasergen, Inc. | 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing |
| NZ590265A (en) | 2008-06-11 | 2012-11-30 | Lasergen Inc | Nucleotides and nucleosides and methods for their use in dna sequencing |
| EP2321429B1 (en) | 2008-09-03 | 2016-08-17 | Quantumdx Group Limited | Methods and kits for nucleic acid sequencing |
| US10759824B2 (en) | 2008-09-03 | 2020-09-01 | Quantumdx Group Limited | Design, synthesis and use of synthetic nucleotides comprising charge mass tags |
| WO2013040257A1 (en) | 2011-09-13 | 2013-03-21 | Lasergen, Inc. | 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing |
| WO2013105025A1 (en) * | 2012-01-13 | 2013-07-18 | Koninklijke Philips N.V. | Dna sequencing with reagent recycling on wiregrid |
| CN103602719B (zh) * | 2013-04-07 | 2016-06-08 | 北京迈基诺基因科技有限责任公司 | 一种基因测序方法 |
| WO2015015913A1 (ja) * | 2013-07-31 | 2015-02-05 | 株式会社 日立ハイテクノロジーズ | 核酸分析用フローセル、及び核酸分析装置 |
| GB201414098D0 (en) | 2014-08-08 | 2014-09-24 | Illumina Cambridge Ltd | Modified nucleotide linkers |
| RU2582198C1 (ru) * | 2014-11-20 | 2016-04-20 | Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) | Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии |
| CN105001292A (zh) * | 2015-07-14 | 2015-10-28 | 深圳市瀚海基因生物科技有限公司 | 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途 |
| EP3400444A4 (en) | 2016-01-04 | 2019-10-16 | QuantuMDx Group Limited | DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS |
| GB201711219D0 (en) | 2017-07-12 | 2017-08-23 | Illumina Cambridge Ltd | Short pendant arm linkers for nucleotides in sequencing applications |
| CN113272443B (zh) | 2019-01-07 | 2025-01-10 | 安捷伦科技有限公司 | 用于单细胞中的基因组dna和基因表达分析的组合物和方法 |
| CN110568039B (zh) * | 2019-09-06 | 2021-05-04 | 中国科学院生物物理研究所 | 使用新型电化学传感器的氨基酸特异实时检测方法 |
| WO2021194640A1 (en) * | 2020-03-26 | 2021-09-30 | Agilent Technologies, Inc. | Methods and compositions for multistage primer extension reactions |
| GB2605404A (en) * | 2021-03-30 | 2022-10-05 | Sumitomo Chemical Co | Sequencing method |
| WO2022263489A1 (en) * | 2021-06-17 | 2022-12-22 | F. Hoffmann-La Roche Ag | Nucleoside-5 -oligophosphates having a cationically-modified nucleobase |
| IL309368A (en) * | 2021-06-29 | 2024-02-01 | Agilent Technologies Inc | polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS |
| CN114250283B (zh) * | 2021-10-15 | 2022-10-04 | 深圳铭毅智造科技有限公司 | 基于环境敏感染料的单色荧光mrt基因测序试剂及方法 |
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| EP0490281A1 (de) * | 1990-12-11 | 1992-06-17 | Hoechst Aktiengesellschaft | 3'- Amino- oder thiolmodifizierte, Fluoreszenzfarbstoff-gekoppelte Nukleoside, Nukleotide und Oligonukleotide sowie ein Verfahren zur Herstellung und ihre Verwendung |
| US5302509A (en) * | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
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| US6211165B1 (en) * | 1997-05-09 | 2001-04-03 | The Trustees Of The University Of Pennsylvania | Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists |
| US6586413B2 (en) * | 1999-11-05 | 2003-07-01 | The United States Of America As Represented By The Department Of Health And Human Services | Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists |
| AU2001296645A1 (en) | 2000-10-06 | 2002-04-15 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding dna and rna |
| DE60212642T2 (de) * | 2001-04-10 | 2007-06-14 | ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE | Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen |
| GB0128526D0 (en) * | 2001-11-29 | 2002-01-23 | Amersham Pharm Biotech Uk Ltd | Nucleotide analogues |
| US7057026B2 (en) * | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| PT3438116T (pt) | 2002-08-23 | 2021-03-23 | Illumina Cambridge Ltd | Nucleótidos identificados |
| CZ294538B6 (cs) * | 2002-12-30 | 2005-01-12 | Ústav Experimentální Botaniky Akademie Vědčeské Re | Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující |
| EP2436778A3 (en) * | 2004-03-03 | 2012-07-11 | The Trustees of Columbia University in the City of New York | Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry |
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2007
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| US5302509A (en) * | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
| EP0490281A1 (de) * | 1990-12-11 | 1992-06-17 | Hoechst Aktiengesellschaft | 3'- Amino- oder thiolmodifizierte, Fluoreszenzfarbstoff-gekoppelte Nukleoside, Nukleotide und Oligonukleotide sowie ein Verfahren zur Herstellung und ihre Verwendung |
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| Emerging technologies in DNA sequencing;Michael L. Metzker;《Genome Res.》;20051231;第15卷;第1767-1776页 * |
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Also Published As
| Publication number | Publication date |
|---|---|
| IL199051A (en) | 2013-11-28 |
| AU2007329361A1 (en) | 2008-06-12 |
| JP5513122B2 (ja) | 2014-06-04 |
| CA2670937C (en) | 2016-07-26 |
| RU2009121089A (ru) | 2011-01-20 |
| MX338089B (es) | 2016-04-01 |
| CN102443030A (zh) | 2012-05-09 |
| BRPI0719923A2 (pt) | 2014-03-04 |
| EP2125856A2 (en) | 2009-12-02 |
| AU2007329361B2 (en) | 2013-07-18 |
| CA2670937A1 (en) | 2008-06-12 |
| KR101504640B1 (ko) | 2015-03-20 |
| BRPI0719923B1 (pt) | 2021-08-17 |
| MX2009005936A (es) | 2010-01-15 |
| WO2008070749A2 (en) | 2008-06-12 |
| JP2011506265A (ja) | 2011-03-03 |
| HK1169413A1 (zh) | 2013-01-25 |
| EP2125856B1 (en) | 2016-09-14 |
| NZ577303A (en) | 2011-12-22 |
| WO2008070749A3 (en) | 2008-11-27 |
| KR20090101451A (ko) | 2009-09-28 |
| EP2125856A4 (en) | 2013-12-25 |
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