JP5513122B2 - 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法 - Google Patents

光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法 Download PDF

Info

Publication number
JP5513122B2
JP5513122B2 JP2009540462A JP2009540462A JP5513122B2 JP 5513122 B2 JP5513122 B2 JP 5513122B2 JP 2009540462 A JP2009540462 A JP 2009540462A JP 2009540462 A JP2009540462 A JP 2009540462A JP 5513122 B2 JP5513122 B2 JP 5513122B2
Authority
JP
Japan
Prior art keywords
compound
mmol
triphosphate
solution
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2009540462A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011506265A5 (https=
JP2011506265A (ja
Inventor
ブラディスラブ エー． リトシュ
ブライアン ピー． ステュピ
マイケル エル． メッツカー
ウェイドン ウー
Original Assignee
レーザーゲン インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/567,193 external-priority patent/US7893227B2/en
Priority claimed from US11/567,189 external-priority patent/US7897737B2/en
Application filed by レーザーゲン インコーポレイテッド filed Critical レーザーゲン インコーポレイテッド
Publication of JP2011506265A publication Critical patent/JP2011506265A/ja
Publication of JP2011506265A5 publication Critical patent/JP2011506265A5/ja
Application granted granted Critical
Publication of JP5513122B2 publication Critical patent/JP5513122B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2009540462A 2006-12-05 2007-12-05 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法 Active JP5513122B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/567,193 US7893227B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US11/567,189 US7897737B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
PCT/US2007/086559 WO2008070749A2 (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2013263830A Division JP5781589B2 (ja) 2013-12-20 2013-12-20 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法

Publications (3)

Publication Number Publication Date
JP2011506265A JP2011506265A (ja) 2011-03-03
JP2011506265A5 JP2011506265A5 (https=) 2012-06-07
JP5513122B2 true JP5513122B2 (ja) 2014-06-04

Family

ID=39493724

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009540462A Active JP5513122B2 (ja) 2006-12-05 2007-12-05 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法

Country Status (12)

Country Link
EP (1) EP2125856B1 (https=)
JP (1) JP5513122B2 (https=)
KR (1) KR101504640B1 (https=)
CN (1) CN102443030B (https=)
AU (1) AU2007329361B2 (https=)
BR (1) BRPI0719923B1 (https=)
CA (1) CA2670937C (https=)
IL (1) IL199051A (https=)
MX (2) MX338089B (https=)
NZ (1) NZ577303A (https=)
RU (1) RU2009121089A (https=)
WO (1) WO2008070749A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013105025A1 (en) * 2012-01-13 2013-07-18 Koninklijke Philips N.V. Dna sequencing with reagent recycling on wiregrid
CN103602719B (zh) * 2013-04-07 2016-06-08 北京迈基诺基因科技有限责任公司 一种基因测序方法
WO2015015913A1 (ja) * 2013-07-31 2015-02-05 株式会社 日立ハイテクノロジーズ 核酸分析用フローセル、及び核酸分析装置
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
RU2582198C1 (ru) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
CN105001292A (zh) * 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN113272443B (zh) 2019-01-07 2025-01-10 安捷伦科技有限公司 用于单细胞中的基因组dna和基因表达分析的组合物和方法
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
WO2021194640A1 (en) * 2020-03-26 2021-09-30 Agilent Technologies, Inc. Methods and compositions for multistage primer extension reactions
GB2605404A (en) * 2021-03-30 2022-10-05 Sumitomo Chemical Co Sequencing method
WO2022263489A1 (en) * 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
IL309368A (en) * 2021-06-29 2024-02-01 Agilent Technologies Inc polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS
CN114250283B (zh) * 2021-10-15 2022-10-04 深圳铭毅智造科技有限公司 基于环境敏感染料的单色荧光mrt基因测序试剂及方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
YU187991A (sh) * 1990-12-11 1994-09-09 Hoechst Aktiengesellschaft 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba
US6211165B1 (en) * 1997-05-09 2001-04-03 The Trustees Of The University Of Pennsylvania Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
US6586413B2 (en) * 1999-11-05 2003-07-01 The United States Of America As Represented By The Department Of Health And Human Services Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
DE60212642T2 (de) * 2001-04-10 2007-06-14 ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
GB0128526D0 (en) * 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
PT3438116T (pt) 2002-08-23 2021-03-23 Illumina Cambridge Ltd Nucleótidos identificados
CZ294538B6 (cs) * 2002-12-30 2005-01-12 Ústav Experimentální Botaniky Akademie Vědčeské Re Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující
EP2436778A3 (en) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry

Also Published As

Publication number Publication date
IL199051A (en) 2013-11-28
AU2007329361A1 (en) 2008-06-12
CA2670937C (en) 2016-07-26
RU2009121089A (ru) 2011-01-20
MX338089B (es) 2016-04-01
CN102443030A (zh) 2012-05-09
BRPI0719923A2 (pt) 2014-03-04
EP2125856A2 (en) 2009-12-02
CN102443030B (zh) 2015-12-16
AU2007329361B2 (en) 2013-07-18
CA2670937A1 (en) 2008-06-12
KR101504640B1 (ko) 2015-03-20
BRPI0719923B1 (pt) 2021-08-17
MX2009005936A (es) 2010-01-15
WO2008070749A2 (en) 2008-06-12
JP2011506265A (ja) 2011-03-03
HK1169413A1 (zh) 2013-01-25
EP2125856B1 (en) 2016-09-14
NZ577303A (en) 2011-12-22
WO2008070749A3 (en) 2008-11-27
KR20090101451A (ko) 2009-09-28
EP2125856A4 (en) 2013-12-25

Similar Documents

Publication Publication Date Title
JP5513122B2 (ja) 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
US7897737B2 (en) 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US8198029B2 (en) 3'-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
JP6196972B2 (ja) ５−メトキシ３’−ｏｈ非遮断高速光開裂性末端ヌクレオチドおよび核酸配列決定のための方法
EP1337541B1 (en) Massive parallel method for decoding DNA and RNA
EP2089343B1 (en) Click chemistry for the production of reporter molecules
JP5143953B2 (ja) ヌクレオチドおよびヌクレオシドならびにｄｎａ配列決定におけるその使用の方法
JPH085908B2 (ja) アルキニルアミノヌクレオチド及びその製造方法
JP2005516595A (ja) ヘテロ配座ポリヌクレオチドおよび使用方法
JP5781589B2 (ja) 光開裂性標識ヌクレオチドとヌクレオシドおよび標識ヌクレオチドとヌクレオシドならびにｄｎａ配列決定におけるそれらの使用法
HK1169413B (en) Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing
HK1163184B (en) Nucleotides and nucleosides and methods for their use in dna sequencing
HK1195076A (en) Nucleotides and nucleosides and methods for their use in dna sequencing
HK1195076B (en) Nucleotides and nucleosides and methods for their use in dna sequencing
CA2754196A1 (en) Massive parallel method for decoding dna and rna
HK1200838B (en) 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101203

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20101203

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120412

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130130

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20130426

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130508

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130730

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20130826

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131220

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140117

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20140212

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140305

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140327

R150 Certificate of patent or registration of utility model

Ref document number: 5513122

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313111

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250