NZ577303A - Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing - Google Patents

Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing

Info

Publication number
NZ577303A
NZ577303A NZ577303A NZ57730307A NZ577303A NZ 577303 A NZ577303 A NZ 577303A NZ 577303 A NZ577303 A NZ 577303A NZ 57730307 A NZ57730307 A NZ 57730307A NZ 577303 A NZ577303 A NZ 577303A
Authority
NZ
New Zealand
Prior art keywords
compound
mmol
triphosphate
nucleic acid
solution
Prior art date
Application number
NZ577303A
Other languages
English (en)
Inventor
Vladislav A Litosh
Brian P Stupi
Michael L Metzker
Weidong Wu
Original Assignee
Lasergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/567,193 external-priority patent/US7893227B2/en
Priority claimed from US11/567,189 external-priority patent/US7897737B2/en
Application filed by Lasergen Inc filed Critical Lasergen Inc
Publication of NZ577303A publication Critical patent/NZ577303A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
NZ577303A 2006-12-05 2007-12-05 Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing NZ577303A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/567,193 US7893227B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
US11/567,189 US7897737B2 (en) 2006-12-05 2006-12-05 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
PCT/US2007/086559 WO2008070749A2 (en) 2006-12-05 2007-12-05 Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing

Publications (1)

Publication Number Publication Date
NZ577303A true NZ577303A (en) 2011-12-22

Family

ID=39493724

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ577303A NZ577303A (en) 2006-12-05 2007-12-05 Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing

Country Status (12)

Country Link
EP (1) EP2125856B1 (https=)
JP (1) JP5513122B2 (https=)
KR (1) KR101504640B1 (https=)
CN (1) CN102443030B (https=)
AU (1) AU2007329361B2 (https=)
BR (1) BRPI0719923B1 (https=)
CA (1) CA2670937C (https=)
IL (1) IL199051A (https=)
MX (2) MX338089B (https=)
NZ (1) NZ577303A (https=)
RU (1) RU2009121089A (https=)
WO (1) WO2008070749A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
NZ590265A (en) 2008-06-11 2012-11-30 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing
EP2321429B1 (en) 2008-09-03 2016-08-17 Quantumdx Group Limited Methods and kits for nucleic acid sequencing
US10759824B2 (en) 2008-09-03 2020-09-01 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising charge mass tags
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013105025A1 (en) * 2012-01-13 2013-07-18 Koninklijke Philips N.V. Dna sequencing with reagent recycling on wiregrid
CN103602719B (zh) * 2013-04-07 2016-06-08 北京迈基诺基因科技有限责任公司 一种基因测序方法
WO2015015913A1 (ja) * 2013-07-31 2015-02-05 株式会社 日立ハイテクノロジーズ 核酸分析用フローセル、及び核酸分析装置
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
RU2582198C1 (ru) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
CN105001292A (zh) * 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
EP3400444A4 (en) 2016-01-04 2019-10-16 QuantuMDx Group Limited DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES WITH LOADING MASS MARKINGS
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
CN113272443B (zh) 2019-01-07 2025-01-10 安捷伦科技有限公司 用于单细胞中的基因组dna和基因表达分析的组合物和方法
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
WO2021194640A1 (en) * 2020-03-26 2021-09-30 Agilent Technologies, Inc. Methods and compositions for multistage primer extension reactions
GB2605404A (en) * 2021-03-30 2022-10-05 Sumitomo Chemical Co Sequencing method
WO2022263489A1 (en) * 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
IL309368A (en) * 2021-06-29 2024-02-01 Agilent Technologies Inc polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS
CN114250283B (zh) * 2021-10-15 2022-10-04 深圳铭毅智造科技有限公司 基于环境敏感染料的单色荧光mrt基因测序试剂及方法

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
YU187991A (sh) * 1990-12-11 1994-09-09 Hoechst Aktiengesellschaft 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba
US6211165B1 (en) * 1997-05-09 2001-04-03 The Trustees Of The University Of Pennsylvania Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
US6586413B2 (en) * 1999-11-05 2003-07-01 The United States Of America As Represented By The Department Of Health And Human Services Methods and compositions for reducing ischemic injury of the heart by administering adenosine receptor agonists and antagonists
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
DE60212642T2 (de) * 2001-04-10 2007-06-14 ECOLE POLYTECHNIQUE FéDéRALE DE LAUSANNE Verfahren zur verwendung von o6-alkylguanin-dna-alkyltransferasen
GB0128526D0 (en) * 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
PT3438116T (pt) 2002-08-23 2021-03-23 Illumina Cambridge Ltd Nucleótidos identificados
CZ294538B6 (cs) * 2002-12-30 2005-01-12 Ústav Experimentální Botaniky Akademie Vědčeské Re Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující
EP2436778A3 (en) * 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry

Also Published As

Publication number Publication date
IL199051A (en) 2013-11-28
AU2007329361A1 (en) 2008-06-12
JP5513122B2 (ja) 2014-06-04
CA2670937C (en) 2016-07-26
RU2009121089A (ru) 2011-01-20
MX338089B (es) 2016-04-01
CN102443030A (zh) 2012-05-09
BRPI0719923A2 (pt) 2014-03-04
EP2125856A2 (en) 2009-12-02
CN102443030B (zh) 2015-12-16
AU2007329361B2 (en) 2013-07-18
CA2670937A1 (en) 2008-06-12
KR101504640B1 (ko) 2015-03-20
BRPI0719923B1 (pt) 2021-08-17
MX2009005936A (es) 2010-01-15
WO2008070749A2 (en) 2008-06-12
JP2011506265A (ja) 2011-03-03
HK1169413A1 (zh) 2013-01-25
EP2125856B1 (en) 2016-09-14
WO2008070749A3 (en) 2008-11-27
KR20090101451A (ko) 2009-09-28
EP2125856A4 (en) 2013-12-25

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Free format text: THE TITLE HAS BEEN CORRECTED TO TITLE: PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHODS FOR THEIR USE IN DNA SEQUENCING; FILING DATE: 01 JUN 2009; STATUS: REJECTED; TITLE: PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHODS FOR THEIR USE IN DNA SEQUENCING; FILING DATE: 26 JUL 2010; STATUS: REJECTED; TITLE: PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHODS FOR THEIR USE IN DNA SEQUENCING; FILING DATE: 09 NOV 2011; STATUS: GRANTED; TITLE: PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHOD

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