BRPI0718989A2 - Ablação genética de células com gene prp usando uma estratégia de captura de promotor direcionada para a produção de proteínas recombinantes isentas de soro como produtos terapêuticos. - Google Patents

Ablação genética de células com gene prp usando uma estratégia de captura de promotor direcionada para a produção de proteínas recombinantes isentas de soro como produtos terapêuticos. Download PDF

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Publication number
BRPI0718989A2
BRPI0718989A2 BRPI0718989-3A2A BRPI0718989A BRPI0718989A2 BR PI0718989 A2 BRPI0718989 A2 BR PI0718989A2 BR PI0718989 A BRPI0718989 A BR PI0718989A BR PI0718989 A2 BRPI0718989 A2 BR PI0718989A2
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BR
Brazil
Prior art keywords
prp
cells
human
cell line
free
Prior art date
Application number
BRPI0718989-3A2A
Other languages
English (en)
Portuguese (pt)
Inventor
Carola Schroeder
Elisabeth Casademunt
Kim Bjoernstrup
Original Assignee
Octagene Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octagene Gmbh filed Critical Octagene Gmbh
Publication of BRPI0718989A2 publication Critical patent/BRPI0718989A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0718989-3A2A 2006-11-20 2007-11-20 Ablação genética de células com gene prp usando uma estratégia de captura de promotor direcionada para a produção de proteínas recombinantes isentas de soro como produtos terapêuticos. BRPI0718989A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06124427 2006-11-20
EP06124427.3 2006-11-20
PCT/EP2007/062597 WO2008061995A2 (en) 2006-11-20 2007-11-20 Genetic ablation of the prp gene cells using a targeted promoter trap strategy for production of serum-free recombinant proteins as therapeuticals

Publications (1)

Publication Number Publication Date
BRPI0718989A2 true BRPI0718989A2 (pt) 2014-02-11

Family

ID=37685785

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0718989-3A2A BRPI0718989A2 (pt) 2006-11-20 2007-11-20 Ablação genética de células com gene prp usando uma estratégia de captura de promotor direcionada para a produção de proteínas recombinantes isentas de soro como produtos terapêuticos.

Country Status (12)

Country Link
EP (1) EP2084269A2 (ko)
KR (1) KR20090106474A (ko)
CN (1) CN101605891A (ko)
AU (1) AU2007324530A1 (ko)
BR (1) BRPI0718989A2 (ko)
CA (1) CA2670003A1 (ko)
IL (1) IL198770A0 (ko)
MX (1) MX2009005277A (ko)
NO (1) NO20091875L (ko)
RU (1) RU2009123458A (ko)
WO (1) WO2008061995A2 (ko)
ZA (1) ZA200903429B (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201503652WA (en) * 2010-05-12 2015-08-28 Janssen Biotech Inc Differentiation of human embryonic stem cells
CN102277379B (zh) * 2011-08-18 2013-07-24 中国科学院遗传与发育生物学研究所 表达凝血因子viii的表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
DE69233477T2 (de) * 1991-11-14 2006-02-16 Prionics Ag Transgene nicht-menschliche tiere ohne prionprotein
DE69928284T2 (de) * 1998-12-22 2006-08-10 The Regents Of The University Of California, Oakland Somatische zellen mit funktionsunfähigem prp gen und verwendungsverfahren
JP2003528620A (ja) * 2000-03-24 2003-09-30 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス プリオンのないトランスジェニック有蹄類
AU2002242854A1 (en) * 2001-03-21 2002-10-03 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals

Also Published As

Publication number Publication date
WO2008061995A2 (en) 2008-05-29
MX2009005277A (es) 2009-05-28
NO20091875L (no) 2009-07-28
RU2009123458A (ru) 2010-12-27
AU2007324530A1 (en) 2008-05-29
ZA200903429B (en) 2010-04-28
IL198770A0 (en) 2011-08-01
EP2084269A2 (en) 2009-08-05
CN101605891A (zh) 2009-12-16
CA2670003A1 (en) 2008-05-29
KR20090106474A (ko) 2009-10-09
WO2008061995A3 (en) 2008-07-10

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