MX2009005277A - Ablacion genetica de celulas del gen prp utilizando una estrategia de trampa promotora dirigida para la produccion de proteinas recombinates sin suero como terapeuticos. - Google Patents

Ablacion genetica de celulas del gen prp utilizando una estrategia de trampa promotora dirigida para la produccion de proteinas recombinates sin suero como terapeuticos.

Info

Publication number
MX2009005277A
MX2009005277A MX2009005277A MX2009005277A MX2009005277A MX 2009005277 A MX2009005277 A MX 2009005277A MX 2009005277 A MX2009005277 A MX 2009005277A MX 2009005277 A MX2009005277 A MX 2009005277A MX 2009005277 A MX2009005277 A MX 2009005277A
Authority
MX
Mexico
Prior art keywords
production
recombinant proteins
therapeuticals
serum
prp gene
Prior art date
Application number
MX2009005277A
Other languages
English (en)
Inventor
Carola Schroeder
Elisabeth Casademunt
Kim Bjoernstrup
Original Assignee
Octagene Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octagene Gmbh filed Critical Octagene Gmbh
Publication of MX2009005277A publication Critical patent/MX2009005277A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención proporciona una línea celular humana somática inmortalizada sin proteína priónica (PrP) en donde ambos alelos del gen PrP han sido completamente eliminados mediante recombinación homóloga. La invención además proporciona un método para la producción de dicha línea celular y su uso para la producción de proteínas recombinantes humanas que son adecuadas como biofarmacéuticos.
MX2009005277A 2006-11-20 2007-11-20 Ablacion genetica de celulas del gen prp utilizando una estrategia de trampa promotora dirigida para la produccion de proteinas recombinates sin suero como terapeuticos. MX2009005277A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06124427 2006-11-20
PCT/EP2007/062597 WO2008061995A2 (en) 2006-11-20 2007-11-20 Genetic ablation of the prp gene cells using a targeted promoter trap strategy for production of serum-free recombinant proteins as therapeuticals

Publications (1)

Publication Number Publication Date
MX2009005277A true MX2009005277A (es) 2009-05-28

Family

ID=37685785

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009005277A MX2009005277A (es) 2006-11-20 2007-11-20 Ablacion genetica de celulas del gen prp utilizando una estrategia de trampa promotora dirigida para la produccion de proteinas recombinates sin suero como terapeuticos.

Country Status (12)

Country Link
EP (1) EP2084269A2 (es)
KR (1) KR20090106474A (es)
CN (1) CN101605891A (es)
AU (1) AU2007324530A1 (es)
BR (1) BRPI0718989A2 (es)
CA (1) CA2670003A1 (es)
IL (1) IL198770A0 (es)
MX (1) MX2009005277A (es)
NO (1) NO20091875L (es)
RU (1) RU2009123458A (es)
WO (1) WO2008061995A2 (es)
ZA (1) ZA200903429B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2569419B1 (en) * 2010-05-12 2019-03-20 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN102277379B (zh) * 2011-08-18 2013-07-24 中国科学院遗传与发育生物学研究所 表达凝血因子viii的表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
ATE288484T1 (de) * 1991-11-14 2005-02-15 Charles Weissmann Transgene nicht-menschliche tiere ohne prionprotein
AU767893B2 (en) * 1998-12-22 2003-11-27 Regents Of The University Of California, The Somatic cells with ablated Prp gene and methods of use
IL151758A0 (en) * 2000-03-24 2003-04-10 Univ Massachusetts Prion-free transgenic ungulates
AU2002242854A1 (en) * 2001-03-21 2002-10-03 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals

Also Published As

Publication number Publication date
WO2008061995A2 (en) 2008-05-29
NO20091875L (no) 2009-07-28
IL198770A0 (en) 2011-08-01
WO2008061995A3 (en) 2008-07-10
CN101605891A (zh) 2009-12-16
AU2007324530A1 (en) 2008-05-29
EP2084269A2 (en) 2009-08-05
KR20090106474A (ko) 2009-10-09
BRPI0718989A2 (pt) 2014-02-11
ZA200903429B (en) 2010-04-28
CA2670003A1 (en) 2008-05-29
RU2009123458A (ru) 2010-12-27

Similar Documents

Publication Publication Date Title
CY1121516T1 (el) ΣΤΕΡΟΕΙΔΕΣ ΠΑΡΑΓΩΓΟ ΓΙΑ ΠΑΡΟΧΗ mRNA ΣΕ ΠΕΡΙΠΤΩΣΕΙΣ ΑΝΘΡΩΠΙΝΩΝ ΓΕΝΕΤΙΚΩΝ ΝΟΣΩΝ
US20210187006A1 (en) Talen-based gene correction
JP6806822B2 (ja) Ｐｐｒモチーフを利用したｄｎａ結合性タンパク質およびその利用
Franz et al. Create and preserve: proteostasis in development and aging is governed by Cdc48/p97/VCP
MX2007003906A (es) Metodos y composiciones para mejorar la produccion de proteinas recombinantes.
MX2007001638A (es) Proteinas de fusion del dominio de unión.
MY156588A (en) Systems using cell culture for production of isoprene
WO2006044492A3 (en) Methods for generating rat embryo-derived cell lines and genetic modification of rat genome
WO2011091350A3 (en) Methods & compositions for improving protein production
NO20072719L (no) Serumfritt cellekulturmedium for pattedyrceller
WO2010051049A3 (en) Thermophilic and thermoacidophilic glycosylation genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
WO2008064306A3 (en) Methods and compositions relating to islet cell neogenesis
WO2005090393A3 (en) Multimeric protein toxins to target cells having multiple identifying characteristics
EP2935319A2 (en) Production of therapeutic proteins in genetically modified mammalian cells
WO2008054514A3 (en) Differential expression profiling analysis of cell culture phenotypes and the uses thereof
EA201070229A1 (ru) Нуклеотидные последовательности msca1, влияющие на мужскую фертильность растений, и способ их применения
ES2901128T3 (es) Proteína no fucosilada y métodos de la misma
EA201170025A1 (ru) Способы повышения жизнеспособности и продукции культуры клеток
WO2007015691A3 (en) Methods and compositions for increasing longevity and protein yield from a cell culture
ES2688727T3 (es) Reducción de la formación de aminoácidos amidados en líneas celulares para la expresión de proteína
MX2009005277A (es) Ablacion genetica de celulas del gen prp utilizando una estrategia de trampa promotora dirigida para la produccion de proteinas recombinates sin suero como terapeuticos.
WO2007053207A3 (en) Methods for controlling stem cell differentiation
TW200738752A (en) Modulation of MDL-1 activity for treatment of inflammatory disease
Wan et al. Identification and characterization of Sl VKOR, a disulfide bond formation protein from Solanum lycopersicum, and bioinformatic analysis of plant VKORs
Miller Mechanisms of Size Control in Pipid Frogs

Legal Events

Date Code Title Description
FA Abandonment or withdrawal