WO2006044492A3 - Methods for generating rat embryo-derived cell lines and genetic modification of rat genome - Google Patents

Methods for generating rat embryo-derived cell lines and genetic modification of rat genome Download PDF

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Publication number
WO2006044492A3
WO2006044492A3 PCT/US2005/036742 US2005036742W WO2006044492A3 WO 2006044492 A3 WO2006044492 A3 WO 2006044492A3 US 2005036742 W US2005036742 W US 2005036742W WO 2006044492 A3 WO2006044492 A3 WO 2006044492A3
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WO
WIPO (PCT)
Prior art keywords
rat
genome
methods
cell lines
genetic modification
Prior art date
Application number
PCT/US2005/036742
Other languages
French (fr)
Other versions
WO2006044492A2 (en
Inventor
Wei He
Wei Weng
Original Assignee
Ingenious Targeting Lab Inc
Wei He
Wei Weng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingenious Targeting Lab Inc, Wei He, Wei Weng filed Critical Ingenious Targeting Lab Inc
Publication of WO2006044492A2 publication Critical patent/WO2006044492A2/en
Publication of WO2006044492A3 publication Critical patent/WO2006044492A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Abstract

The present invention provides novel methods for isolating rat embryo-derived cells and modifying the genome of the rat cells which typically comprise the steps of: constructing a DNA molecule in which desired sequence modifications are contained in a segment of DNA (a 'targeting DNA); introducing the targeting DNA construct into the cell (e.g., by microinjection, electroporation, transfection, or calcium phosphate precipitation); and selecting cells in which the desired sequence modifications have been introduced into the genome via homologous recombination.
PCT/US2005/036742 2004-10-14 2005-10-14 Methods for generating rat embryo-derived cell lines and genetic modification of rat genome WO2006044492A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61883804P 2004-10-14 2004-10-14
US60/618,838 2004-10-14

Publications (2)

Publication Number Publication Date
WO2006044492A2 WO2006044492A2 (en) 2006-04-27
WO2006044492A3 true WO2006044492A3 (en) 2006-08-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/036742 WO2006044492A2 (en) 2004-10-14 2005-10-14 Methods for generating rat embryo-derived cell lines and genetic modification of rat genome

Country Status (1)

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WO (1) WO2006044492A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8722708B2 (en) 2005-06-14 2014-05-13 Merck Sharp & Dohme Inc. Substituted isoindolines as aspartyl protease inhibitors
CA2609582A1 (en) 2005-06-14 2006-12-28 Schering Corporation Aspartyl protease inhibitors
ATE489370T1 (en) 2005-06-14 2010-12-15 Schering Corp PREPARATION AND USE OF COMPOUNDS AS ASPARTYL PROTEASE INHIBITORS
AU2007332754A1 (en) 2006-12-12 2008-06-19 Schering Corporation Aspartyl protease inhibitors
US20120204278A1 (en) 2009-07-08 2012-08-09 Kymab Limited Animal models and therapeutic molecules
US9445581B2 (en) 2012-03-28 2016-09-20 Kymab Limited Animal models and therapeutic molecules
SI3241435T1 (en) 2009-07-08 2021-11-30 Kymab Limited, Animal models and therapeutic molecules
AU2012311288B2 (en) 2011-09-19 2017-08-17 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
EP2761008A1 (en) 2011-09-26 2014-08-06 Kymab Limited Chimaeric surrogate light chains (slc) comprising human vpreb
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
US10251377B2 (en) 2012-03-28 2019-04-09 Kymab Limited Transgenic non-human vertebrate for the expression of class-switched, fully human, antibodies
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
US9788534B2 (en) 2013-03-18 2017-10-17 Kymab Limited Animal models and therapeutic molecules
US9783618B2 (en) 2013-05-01 2017-10-10 Kymab Limited Manipulation of immunoglobulin gene diversity and multi-antibody therapeutics
US11707056B2 (en) 2013-05-02 2023-07-25 Kymab Limited Animals, repertoires and methods
US9783593B2 (en) 2013-05-02 2017-10-10 Kymab Limited Antibodies, variable domains and chains tailored for human use
JP7133902B2 (en) 2013-10-01 2022-09-09 カイマブ・リミテッド Animal models and therapeutic molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VASSILIEVA S.: "Establishment of SSEA-1 and Oct-4-Expressing rat embryonic stem-like cell lines and effects of cytokines of the IL-6 family on clonal growth", EXPERIMENTAL CELL RESEARCH, vol. 258, 2000, pages 361 - 373, XP002232587 *

Also Published As

Publication number Publication date
WO2006044492A2 (en) 2006-04-27

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