BR9508053A - Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga - Google Patents

Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga

Info

Publication number
BR9508053A
BR9508053A BR9508053A BR9508053A BR9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A
Authority
BR
Brazil
Prior art keywords
construction
production
heterologous protein
yeast strain
vector dna
Prior art date
Application number
BR9508053A
Other languages
English (en)
Portuguese (pt)
Inventor
Thomas Borglum Kjeldsen
Knud Vad
Jokob Brandt
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9508053A publication Critical patent/BR9508053A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
BR9508053A 1994-06-17 1995-06-16 Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga BR9508053A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (1)

Publication Number Publication Date
BR9508053A true BR9508053A (pt) 1997-08-12

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9508053A BR9508053A (pt) 1994-06-17 1995-06-16 Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga

Country Status (16)

Country Link
EP (1) EP0765395A1 (sv)
JP (1) JP3730255B2 (sv)
CN (1) CN1131312C (sv)
AU (1) AU2733595A (sv)
BR (1) BR9508053A (sv)
CA (1) CA2192943A1 (sv)
CZ (1) CZ363796A3 (sv)
FI (1) FI965030A (sv)
HU (1) HUT75840A (sv)
IL (1) IL114160A (sv)
MX (1) MX9606536A (sv)
NO (1) NO965411L (sv)
PL (1) PL317761A1 (sv)
SG (1) SG47883A1 (sv)
WO (1) WO1995035384A1 (sv)
ZA (1) ZA954983B (sv)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP1365028B1 (en) * 1996-12-13 2009-03-18 Novartis Vaccines and Diagnostics, Inc. Method for expression of PDGF or IGF proteins in yeast
EP0946735B1 (en) * 1996-12-20 2006-02-08 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
JP2003525570A (ja) 1998-01-23 2003-09-02 ノボ ノルディスク アクティーゼルスカブ 酵母において所望のポリペプチドを産生する方法
PL356005A1 (en) 1999-12-29 2004-05-31 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
EP1421102A2 (en) * 2001-07-24 2004-05-26 Novo Nordisk A/S Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
ATE517119T1 (de) 2003-12-03 2011-08-15 Novo Nordisk As Einzelketteninsulin
WO2007020256A1 (en) 2005-08-16 2007-02-22 Novo Nordisk A/S Method for making mature insulin polypeptides
JP5550338B2 (ja) * 2006-07-31 2014-07-16 ノボ・ノルデイスク・エー／エス ペグ化持続型インスリン
CN101541830A (zh) 2006-09-22 2009-09-23 诺沃-诺迪斯克有限公司 蛋白酶抗性的胰岛素类似物
WO2008037735A1 (en) 2006-09-27 2008-04-03 Novo Nordisk A/S Method for making maturated insulin polypeptides
ES2425413T3 (es) 2006-11-22 2013-10-15 Novo Nordisk A/S Método para preparar carboxipeptidasa activada
EP2170945A1 (en) 2007-07-16 2010-04-07 Novo Nordisk A/S Protease stabilized, pegylated insulin analogues
EP2178909B1 (en) 2007-08-13 2015-10-21 Novo Nordisk A/S Rapid acting insulin analogues
CN101784562B (zh) 2007-08-15 2016-07-13 诺沃-诺迪斯克有限公司 具有酰基和亚烷基二醇部分的胰岛素类似物
WO2009022006A1 (en) 2007-08-15 2009-02-19 Novo Nordisk A/S Insulins with an acyl moiety comprising repeating units of alkylene glycol containing amino acids
EP2910571B1 (en) 2008-03-18 2016-10-05 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
JP5818681B2 (ja) 2008-04-01 2015-11-18 ノボ・ノルデイスク・エー／エス インスリンアルブミンコンジュゲート
PL2340033T3 (pl) 2009-06-26 2014-03-31 Novo Nordisk As Preparat zawierający insulinę, nikotynamid i argininę
ES2550761T3 (es) 2009-11-25 2015-11-12 Novo Nordisk A/S Método para producción de polipéptidos
WO2011161083A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Human insulin containing additional disulfide bonds
BR112012033107A2 (pt) 2010-06-23 2016-10-11 Novo Nordisk As derivados de insulina contendo ligações dissulfeto adicionais.
JP5973427B2 (ja) 2010-06-23 2016-08-23 ノヴォ ノルディスク アー／エス 追加のジスルフィド結合を含有するインスリン類似体
KR20140030125A (ko) 2010-12-14 2014-03-11 노보 노르디스크 에이/에스 인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
US20130331320A1 (en) 2010-12-14 2013-12-12 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
WO2012171994A1 (en) 2011-06-15 2012-12-20 Novo Nordisk A/S Multi substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
EP2925345B1 (en) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
WO2014195452A1 (en) 2013-06-07 2014-12-11 Novo Nordisk A/S Method for making mature insulin polypeptides
WO2015128507A1 (en) 2014-02-28 2015-09-03 Novo Nordisk A/S Mating factor alpha pro-peptide variants
EP3303380B1 (en) 2015-06-02 2020-01-15 Novo Nordisk A/S Insulins with polar recombinant extensions
US20180244743A1 (en) 2015-08-25 2018-08-30 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
JP2018531900A (ja) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス 新規インスリン誘導体及びその医学的使用
EP3341401A1 (en) 2015-08-25 2018-07-04 Novo Nordisk A/S Novel insulin derivatives and the medical uses hereof
JP7013390B2 (ja) * 2016-05-24 2022-01-31 ノヴォ ノルディスク アー／エス Ｍｉｃ－１化合物及びその使用
EP3668892A1 (en) 2017-08-17 2020-06-24 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
JP2021532730A (ja) * 2018-06-18 2021-12-02 ユニケム ラボラトリーズ リミテッド 組換えタンパク質の発現レベルを高めるためのリーダー配列
BR112022009510A2 (pt) 2019-12-11 2022-08-16 Novo Nordisk As Análogo de insulina, composição farmacêutica, e, método de tratamento, prevenção ou alívio de uma doença ou distúrbio ou condição
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
JPH10501695A (ja) 1998-02-17
CN1131312C (zh) 2003-12-17
PL317761A1 (en) 1997-04-28
IL114160A (en) 2006-12-31
NO965411D0 (no) 1996-12-16
FI965030A (sv) 1997-02-14
MX9606536A (es) 1997-03-29
CN1152942A (zh) 1997-06-25
ZA954983B (en) 1996-02-14
NO965411L (no) 1997-02-14
CZ363796A3 (en) 1997-05-14
FI965030A0 (sv) 1996-12-16
HU9603476D0 (en) 1997-02-28
CA2192943A1 (en) 1995-12-28
AU2733595A (en) 1996-01-15
IL114160A0 (en) 1995-10-31
WO1995035384A1 (en) 1995-12-28
JP3730255B2 (ja) 2005-12-21
HUT75840A (en) 1997-05-28
SG47883A1 (en) 1998-04-17
EP0765395A1 (en) 1997-04-02

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law