BR122020012639B8 - Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho - Google Patents

Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho

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Publication number
BR122020012639B8
BR122020012639B8 BR122020012639A BR122020012639A BR122020012639B8 BR 122020012639 B8 BR122020012639 B8 BR 122020012639B8 BR 122020012639 A BR122020012639 A BR 122020012639A BR 122020012639 A BR122020012639 A BR 122020012639A BR 122020012639 B8 BR122020012639 B8 BR 122020012639B8
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BR
Brazil
Prior art keywords
type
gene
plant
methods
male sterility
Prior art date
Application number
BR122020012639A
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English (en)
Other versions
BR122020012639B1 (pt
Inventor
Ren Ruihua
A Nagel Bruce
P Kumpatla Siva
Zheng Peizhong
L Cutter Gary
W Greene Thomas
A Thompson Steven
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Publication of BR122020012639B1 publication Critical patent/BR122020012639B1/pt
Publication of BR122020012639B8 publication Critical patent/BR122020012639B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A01H1/023—Male sterility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Output Control And Ontrol Of Special Type Engine (AREA)

Abstract

A presente invenção refere-se ao mapeamento de alta reso-lução e clonagem de gene candidato de Rf4, um gene restaurador de fertilidade do milho que restaura a fertilidade à esterilidade masculina citoplasmática de tipo C. A descrição também se refere a marcadores moleculares que estão firmemente ligados a ou residem dentro do ge-ne Rf4. Em algumas modalidades, apresentam-se métodos pelos quais sementes híbridas podem ser produzidas a partir de cruzamen-tos de uma planta masculina compreendendo marcadores moleculares de ácido nucleico que estejam ligados ou que residam dentro do gene Rf4 e uma planta feminina portadora de CMS tipo C.
BR122020012639A 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho BR122020012639B8 (pt)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US39052610P 2010-10-06 2010-10-06
US61/390,526 2010-10-06
US13/244,049 2011-09-23
US13/244,049 US10117411B2 (en) 2010-10-06 2011-09-23 Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use
BR112013008387A BR112013008387B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho
PCT/US2011/053304 WO2012047595A2 (en) 2010-10-06 2011-09-26 Maize cytoplasmic male sterility (cms) c-type restorer rf4 gene, molecular markers and their use

Publications (2)

Publication Number Publication Date
BR122020012639B1 BR122020012639B1 (pt) 2021-04-27
BR122020012639B8 true BR122020012639B8 (pt) 2022-10-11

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BR122020012639A BR122020012639B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho
BR112013008387A BR112013008387B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho

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Country Status (18)

Country Link
US (1) US10117411B2 (pt)
EP (2) EP3018217B1 (pt)
CN (2) CN103249845B (pt)
AR (1) AR083309A1 (pt)
AU (2) AU2011312559B2 (pt)
BR (2) BR122020012639B8 (pt)
CA (1) CA2812576C (pt)
CL (2) CL2013000911A1 (pt)
ES (1) ES2744675T3 (pt)
HK (2) HK1219299A1 (pt)
HU (1) HUE045078T2 (pt)
MX (1) MX349350B (pt)
NZ (2) NZ608572A (pt)
RU (3) RU2747979C2 (pt)
UA (1) UA114886C2 (pt)
UY (1) UY33654A (pt)
WO (1) WO2012047595A2 (pt)
ZA (1) ZA201302204B (pt)

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UY35927A (es) 2013-12-31 2015-07-31 Dow Agrosciences Llc ?gen restaurador rf3 de tipo s de la esterilidad masculina citoplasmática del maíz (cms), marcadores moleculares y sus usos?.
UY35928A (es) 2013-12-31 2015-07-31 Dow Agrosciences Llc ?GEN Rf3 RESTAURADOR DE LA ANDROESTERILIDAD CITOPLASMÁTICA (CMS) TIPO S?.
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CN107699631B (zh) * 2017-11-17 2021-09-10 青海省农林科学院 甘蓝型油菜雌雄不育系Bnmfs利用分子标记筛选的引物及方法
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US11472991B2 (en) 2019-07-31 2022-10-18 King Fahd University Of Petroleum And Minerals Method of producing alumina ceramics reinforced with oil fly ash
KR102199454B1 (ko) * 2019-12-23 2021-01-06 충북대학교 산학협력단 배의 세포질 웅성불임 판별용 분자마커 및 이의 용도
CN111575398B (zh) * 2020-05-28 2022-05-03 北京市农林科学院 玉米第4号染色体雄性不育相关基因的分子标记及其应用
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ZA201302204B (en) 2014-05-28
EP2625291A4 (en) 2014-04-23
NZ707345A (en) 2017-01-27
AU2011312559B2 (en) 2016-05-19
EP2625291A2 (en) 2013-08-14
RU2603005C2 (ru) 2016-11-20
RU2747979C2 (ru) 2021-05-18
CN103249845B (zh) 2015-09-16
US10117411B2 (en) 2018-11-06
RU2016114532A (ru) 2018-11-28
ES2744675T3 (es) 2020-02-25
MX2013003917A (es) 2013-06-05
EP3018217B1 (en) 2019-06-05
BR112013008387B1 (pt) 2021-04-20
US20120090047A1 (en) 2012-04-12
EP3018217A3 (en) 2016-08-17
UA114886C2 (uk) 2017-08-28
WO2012047595A3 (en) 2012-08-16
RU2729107C2 (ru) 2020-08-04
AR083309A1 (es) 2013-02-13
CA2812576A1 (en) 2012-04-12
CL2013000911A1 (es) 2014-05-16
RU2016136834A (ru) 2018-12-11
RU2016114532A3 (pt) 2019-12-24
CL2016000546A1 (es) 2016-09-02
AU2011312559A1 (en) 2013-04-11
BR112013008387A2 (pt) 2016-06-14
RU2016136834A3 (pt) 2020-08-10
MX349350B (es) 2017-07-24
HK1219299A1 (zh) 2017-03-31
HUE045078T2 (hu) 2019-12-30
AU2016216734B2 (en) 2018-01-25
HK1188258A1 (zh) 2014-04-25
RU2013119981A (ru) 2014-11-20
WO2012047595A2 (en) 2012-04-12
AU2016216734A1 (en) 2016-09-08
BR112013008387B8 (pt) 2022-10-11
BR122020012639B1 (pt) 2021-04-27
NZ608572A (en) 2015-07-31
CN105177122A (zh) 2015-12-23
UY33654A (es) 2012-04-30
CN103249845A (zh) 2013-08-14
EP3018217A2 (en) 2016-05-11
CA2812576C (en) 2020-04-28

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