UA117346C2 - СПОСІБ ВИЗНАЧЕННЯ НАЯВНОСТІ/ВІДСУТНОСТІ МУТАЦІЇ fl2 В РОСЛИНІ КУКУРУДЗИ - Google Patents

СПОСІБ ВИЗНАЧЕННЯ НАЯВНОСТІ/ВІДСУТНОСТІ МУТАЦІЇ fl2 В РОСЛИНІ КУКУРУДЗИ

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Publication number
UA117346C2
UA117346C2 UAA201414105A UAA201414105A UA117346C2 UA 117346 C2 UA117346 C2 UA 117346C2 UA A201414105 A UAA201414105 A UA A201414105A UA A201414105 A UAA201414105 A UA A201414105A UA 117346 C2 UA117346 C2 UA 117346C2
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UA
Ukraine
Prior art keywords
floury
gene
maize
plants
specific assay
Prior art date
Application number
UAA201414105A
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English (en)
Inventor
Вей Чень
Натан ВАНОПДОРП
Стів Дж. Плен
Пітер Фрідеманн (померлий)
Надія Чаідір
Сіва Прасад Кумпатла
Original Assignee
ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі
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Application filed by ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі filed Critical ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі
Publication of UA117346C2 publication Critical patent/UA117346C2/uk

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Винахід стосується способу визначення наявності/відсутності мутації fl2 із застосуванням рослинної тканини кукурудзи на основі методів гібридизації та ПЛР. Винахід також стосується генетично сконструйованої рослини кукурудзи, що містить SNP C/T та молекули нуклеїнової кислоти, яка кодує білок альфа-зеїн.
UAA201414105A 2012-05-30 2013-05-29 СПОСІБ ВИЗНАЧЕННЯ НАЯВНОСТІ/ВІДСУТНОСТІ МУТАЦІЇ fl2 В РОСЛИНІ КУКУРУДЗИ UA117346C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653287P 2012-05-30 2012-05-30
PCT/US2013/042997 WO2015012783A2 (en) 2012-05-30 2013-05-29 Floury 2 gene-specific assay in maize for floury (fl2) trait introgression

Publications (1)

Publication Number Publication Date
UA117346C2 true UA117346C2 (uk) 2018-07-25

Family

ID=52393922

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201414105A UA117346C2 (uk) 2012-05-30 2013-05-29 СПОСІБ ВИЗНАЧЕННЯ НАЯВНОСТІ/ВІДСУТНОСТІ МУТАЦІЇ fl2 В РОСЛИНІ КУКУРУДЗИ

Country Status (11)

Country Link
US (1) US9994919B2 (uk)
EP (1) EP2855709A4 (uk)
CN (1) CN104837985B (uk)
AR (1) AR091214A1 (uk)
BR (1) BR112014029941A2 (uk)
CA (1) CA2882079A1 (uk)
MX (1) MX362337B (uk)
NZ (1) NZ703141A (uk)
RU (1) RU2661110C2 (uk)
UA (1) UA117346C2 (uk)
WO (1) WO2015012783A2 (uk)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3547844A4 (en) * 2016-12-02 2020-07-29 Agrigenetics, Inc. SILAGE AND ANIMAL FEED COMPOSITIONS MADE THEREOF FROM MAIZE HYBRID WITH BROWN MEDIUM RIB AND MINE CHARACTERISTICS

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2181418A1 (en) 1996-07-17 1998-01-18 Larry Beach Transgenes with floury2 gene signal peptide
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
NZ547492A (en) * 2003-10-28 2009-12-24 Bioarray Solutions Ltd Optimization of gene expression analysis using immobilized capture probes of different lengths and densities
CN101124333A (zh) * 2003-10-28 2008-02-13 佰尔瑞溶液有限公司 使用固定化的捕获探针优化基因表达分析
US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
US20110105347A1 (en) * 2007-05-17 2011-05-05 Monsanto Technology Llc Corn polymorphisms and methods of genotyping
WO2009123946A1 (en) * 2008-03-31 2009-10-08 Monsanto Technology Llc Novel opaque modifiers in corn and related methods
WO2011143344A2 (en) * 2010-05-12 2011-11-17 Agrigenetics, Inc. Use of brown midrib-3 gene specific markers in maize for trait introgression

Also Published As

Publication number Publication date
WO2015012783A3 (en) 2015-03-26
US20160153055A1 (en) 2016-06-02
US9994919B2 (en) 2018-06-12
BR112014029941A2 (pt) 2017-09-12
MX362337B (es) 2019-01-11
CN104837985B (zh) 2019-10-25
EP2855709A4 (en) 2016-04-20
CN104837985A (zh) 2015-08-12
CA2882079A1 (en) 2015-01-29
NZ703141A (en) 2017-06-30
EP2855709A2 (en) 2015-04-08
WO2015012783A2 (en) 2015-01-29
MX2014014629A (es) 2015-10-12
RU2661110C2 (ru) 2018-07-11
AR091214A1 (es) 2015-01-21
RU2014152692A (ru) 2016-07-20

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