BR112014029941A2 - ensaio específico de gene floury 2 em milho para introgressão de traço de floury (fl2) - Google Patents

ensaio específico de gene floury 2 em milho para introgressão de traço de floury (fl2)

Info

Publication number
BR112014029941A2
BR112014029941A2 BR112014029941A BR112014029941A BR112014029941A2 BR 112014029941 A2 BR112014029941 A2 BR 112014029941A2 BR 112014029941 A BR112014029941 A BR 112014029941A BR 112014029941 A BR112014029941 A BR 112014029941A BR 112014029941 A2 BR112014029941 A2 BR 112014029941A2
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BR
Brazil
Prior art keywords
floury
maize
introgression
trace
assay
Prior art date
Application number
BR112014029941A
Other languages
English (en)
Inventor
Chaidir Nadia
Vanopdorp Nathan
Friedemann Peter (Falecido)
Prasad Kumpatla Siva
J Plehn Steve
Chen Wei
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112014029941A2 publication Critical patent/BR112014029941A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

resumo patente de invenção: "ensaio específico de gene floury 2 em milho para introgressão de traço de floury (fl2)". a revelação refere-se a composições e métodos para determinação da zigosidade de plantas de milho contendo uma ou mais mutações de floury2 (fl2). nas concretizações, a revelação se refere a um ensaio molecular à base de pcr específico de gene (kaspar®) para identificação do traço de floury2 em plantas. em certas concretizações, as composições e métodos são revelados para determinação da zigosidade de plantas de milho com relação ao alelo de fl2. em concretizações particulares, o ensaio pode ser usado para a identificação do germoplasma de fl2, aceleração da introgressão e programas de reprodução molecular em milho e outras plantas.
BR112014029941A 2012-05-30 2013-05-29 ensaio específico de gene floury 2 em milho para introgressão de traço de floury (fl2) BR112014029941A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653287P 2012-05-30 2012-05-30
PCT/US2013/042997 WO2015012783A2 (en) 2012-05-30 2013-05-29 Floury 2 gene-specific assay in maize for floury (fl2) trait introgression

Publications (1)

Publication Number Publication Date
BR112014029941A2 true BR112014029941A2 (pt) 2017-09-12

Family

ID=52393922

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014029941A BR112014029941A2 (pt) 2012-05-30 2013-05-29 ensaio específico de gene floury 2 em milho para introgressão de traço de floury (fl2)

Country Status (11)

Country Link
US (1) US9994919B2 (pt)
EP (1) EP2855709A4 (pt)
CN (1) CN104837985B (pt)
AR (1) AR091214A1 (pt)
BR (1) BR112014029941A2 (pt)
CA (1) CA2882079A1 (pt)
MX (1) MX362337B (pt)
NZ (1) NZ703141A (pt)
RU (1) RU2661110C2 (pt)
UA (1) UA117346C2 (pt)
WO (1) WO2015012783A2 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11039628B2 (en) 2016-12-02 2021-06-22 Agrigenetics, Inc. Methods of using silage produced from a corn hybrid comprising brown midrib and floury traits for meat production

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2181418A1 (en) * 1996-07-17 1998-01-18 Larry Beach Transgenes with floury2 gene signal peptide
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
CA2544041C (en) * 2003-10-28 2015-12-08 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
CN101124333A (zh) * 2003-10-28 2008-02-13 佰尔瑞溶液有限公司 使用固定化的捕获探针优化基因表达分析
US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
EP2147012A4 (en) * 2007-05-17 2011-03-02 Monsanto Technology Llc MAISPOYLMORPHISMS AND METHOD FOR THEIR GENOTYPIZATION
US8299335B2 (en) * 2008-03-31 2012-10-30 Monsanto Technology Llc Corn seed comprising novel opaque modifiers and related methods
US9222141B2 (en) 2010-05-12 2015-12-29 Agrigenetics, Inc. Use of brown midrib-3 gene specific markers in maize for trait introgression

Also Published As

Publication number Publication date
EP2855709A2 (en) 2015-04-08
CA2882079A1 (en) 2015-01-29
EP2855709A4 (en) 2016-04-20
RU2661110C2 (ru) 2018-07-11
WO2015012783A3 (en) 2015-03-26
CN104837985A (zh) 2015-08-12
MX362337B (es) 2019-01-11
WO2015012783A2 (en) 2015-01-29
NZ703141A (en) 2017-06-30
AR091214A1 (es) 2015-01-21
RU2014152692A (ru) 2016-07-20
US20160153055A1 (en) 2016-06-02
CN104837985B (zh) 2019-10-25
US9994919B2 (en) 2018-06-12
MX2014014629A (es) 2015-10-12
UA117346C2 (uk) 2018-07-25

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements