MX362337B - Ensayo especifico al gene harinoso-2 en maiz para la introgresion del rasgo harinoso (fl2). - Google Patents

Ensayo especifico al gene harinoso-2 en maiz para la introgresion del rasgo harinoso (fl2).

Info

Publication number
MX362337B
MX362337B MX2014014629A MX2014014629A MX362337B MX 362337 B MX362337 B MX 362337B MX 2014014629 A MX2014014629 A MX 2014014629A MX 2014014629 A MX2014014629 A MX 2014014629A MX 362337 B MX362337 B MX 362337B
Authority
MX
Mexico
Prior art keywords
floury
gene
maize
plants
specific assay
Prior art date
Application number
MX2014014629A
Other languages
English (en)
Other versions
MX2014014629A (es
Inventor
Chen Wei
Chaidir Nadia
P Kumpatla Siva
Vanopdorp Nathan
Friedemann Peter
Plehn Steve
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of MX2014014629A publication Critical patent/MX2014014629A/es
Publication of MX362337B publication Critical patent/MX362337B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La descripción se refiere a composiciones y métodos para determinar la cigocidad de las plantas de maíz que contiene una o más mutaciones a harinoso-2 (f12). En modalidades, la descripción se refiere a un ensayo molecular a base de PCR específico al gen (KASPar(r)) para identificar el rasgo harinoso-2 en plantas. En ciertas modalidades, se describen composiciones y métodos para determinar la cigocidad de las plantas de maíz con respecto al alelo fl2. En modalidades particulares el ensayo puede ser usado para la identificación del germoplasma fl2, acelerando la introgresión y los programas de cultivo molecular en maíz y otras plantas.
MX2014014629A 2012-05-30 2013-05-29 Ensayo especifico al gene harinoso-2 en maiz para la introgresion del rasgo harinoso (fl2). MX362337B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653287P 2012-05-30 2012-05-30
PCT/US2013/042997 WO2015012783A2 (en) 2012-05-30 2013-05-29 Floury 2 gene-specific assay in maize for floury (fl2) trait introgression

Publications (2)

Publication Number Publication Date
MX2014014629A MX2014014629A (es) 2015-10-12
MX362337B true MX362337B (es) 2019-01-11

Family

ID=52393922

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014014629A MX362337B (es) 2012-05-30 2013-05-29 Ensayo especifico al gene harinoso-2 en maiz para la introgresion del rasgo harinoso (fl2).

Country Status (11)

Country Link
US (1) US9994919B2 (es)
EP (1) EP2855709A4 (es)
CN (1) CN104837985B (es)
AR (1) AR091214A1 (es)
BR (1) BR112014029941A2 (es)
CA (1) CA2882079A1 (es)
MX (1) MX362337B (es)
NZ (1) NZ703141A (es)
RU (1) RU2661110C2 (es)
UA (1) UA117346C2 (es)
WO (1) WO2015012783A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018102674A1 (en) * 2016-12-02 2018-06-07 Agrigenetics, Inc. Silage produced from a corn hybrid comprising brown midrib and floury traits, and animal feed compositions comprising same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2181418A1 (en) * 1996-07-17 1998-01-18 Larry Beach Transgenes with floury2 gene signal peptide
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
CN101124333A (zh) * 2003-10-28 2008-02-13 佰尔瑞溶液有限公司 使用固定化的捕获探针优化基因表达分析
CA2899287A1 (en) * 2003-10-28 2005-05-12 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
US7157281B2 (en) * 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
EP2147012A4 (en) * 2007-05-17 2011-03-02 Monsanto Technology Llc MAISPOYLMORPHISMS AND METHOD FOR THEIR GENOTYPIZATION
WO2009123946A1 (en) * 2008-03-31 2009-10-08 Monsanto Technology Llc Novel opaque modifiers in corn and related methods
EP2569448B1 (en) 2010-05-12 2017-01-04 Agrigenetics, Inc. Use of brown midrib-3 gene specific markers in maize for trait introgression

Also Published As

Publication number Publication date
UA117346C2 (uk) 2018-07-25
CN104837985A (zh) 2015-08-12
BR112014029941A2 (pt) 2017-09-12
RU2661110C2 (ru) 2018-07-11
RU2014152692A (ru) 2016-07-20
MX2014014629A (es) 2015-10-12
EP2855709A4 (en) 2016-04-20
NZ703141A (en) 2017-06-30
WO2015012783A2 (en) 2015-01-29
EP2855709A2 (en) 2015-04-08
AR091214A1 (es) 2015-01-21
US9994919B2 (en) 2018-06-12
WO2015012783A3 (en) 2015-03-26
US20160153055A1 (en) 2016-06-02
CA2882079A1 (en) 2015-01-29
CN104837985B (zh) 2019-10-25

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