BR112013008387B8 - Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho - Google Patents

Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho

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Publication number
BR112013008387B8
BR112013008387B8 BR112013008387A BR112013008387A BR112013008387B8 BR 112013008387 B8 BR112013008387 B8 BR 112013008387B8 BR 112013008387 A BR112013008387 A BR 112013008387A BR 112013008387 A BR112013008387 A BR 112013008387A BR 112013008387 B8 BR112013008387 B8 BR 112013008387B8
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Prior art keywords
gene
plant
corn
type
methods
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BR112013008387A
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English (en)
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BR112013008387B1 (pt
BR112013008387A2 (pt
Inventor
Ren Ruihua
A Nagel Bruce
Zheng Peizhong
L Cutter Gary
W Greene Thomas
A Thompson Steven
P Kumpatla Siva
Original Assignee
Dow Agrosciences Llc
Corteva Agriscience Llc
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Publication date
Application filed by Dow Agrosciences Llc, Corteva Agriscience Llc filed Critical Dow Agrosciences Llc
Priority to BR122020012639A priority Critical patent/BR122020012639B8/pt
Publication of BR112013008387A2 publication Critical patent/BR112013008387A2/pt
Publication of BR112013008387B1 publication Critical patent/BR112013008387B1/pt
Publication of BR112013008387B8 publication Critical patent/BR112013008387B8/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/022—Genic fertility modification, e.g. apomixis
    • A01H1/023—Male sterility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Output Control And Ontrol Of Special Type Engine (AREA)

Abstract

GENE RF4 RESTAURADOR DA ESTERILIDADE MASCULINA CITOPLASMÁTICA (CMS) DE TIPO C DO MILHO, ÁCIDO NUCLEICO E MÉTODOS PARA IDENTIFICAÇÃO DE UMA PLANTA COMPREENDENDO O REFERIDO GENE, ISOLAMENTO DO MESMO, RESTAURAR A FERTILIDADE NO MILHO, PRODUÇÃO DE SEMENTES DE MILHO HÍBRIDAS, PRODUÇÃO DE UM ORGANISMO GENETICAMENTE MANIPULADO, ISOLAMENTO DE UM HOMÓLOGO DE RF4 DE UMA PLANTA E INTRODUÇÃO DO GENE RF4-BHLH DE MILHO EM UM ORGANISMO HOSPEDEIRO. A presente invenção refere-se ao mapeamento de alta resolução e clonagem de gene candidato de Rf4, um gene restaurador de fertilidade do milho que restaura a fertilidade à esterilidade masculina citoplasmática de tipo C. A descrição também se refere a marcadores moleculares que estão firmemente ligados a ou residem dentro do gene Rf4. Em algumas modalidades, apresentam-se métodos pelos quais sementes híbridas podem ser produzidas a partir de cruzamentos de uma planta masculina compreendendo marcadores moleculares de ácido nucleico que estejam ligados ou que residam dentro do gene Rf4 e uma planta feminina portadora de CMS tipo C.
BR112013008387A 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho BR112013008387B8 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122020012639A BR122020012639B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US39052610P 2010-10-06 2010-10-06
US61/390,526 2010-10-06
US13/244,049 2011-09-23
US13/244,049 US10117411B2 (en) 2010-10-06 2011-09-23 Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use
PCT/US2011/053304 WO2012047595A2 (en) 2010-10-06 2011-09-26 Maize cytoplasmic male sterility (cms) c-type restorer rf4 gene, molecular markers and their use

Publications (3)

Publication Number Publication Date
BR112013008387A2 BR112013008387A2 (pt) 2016-06-14
BR112013008387B1 BR112013008387B1 (pt) 2021-04-20
BR112013008387B8 true BR112013008387B8 (pt) 2022-10-11

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BR122020012639A BR122020012639B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho
BR112013008387A BR112013008387B8 (pt) 2010-10-06 2011-09-26 Métodos para a identificação de uma planta compreendendo um gene restaurador funcional para esterilidade masculina citoplasmática de tipo c do milho

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Country Status (18)

Country Link
US (1) US10117411B2 (pt)
EP (2) EP3018217B1 (pt)
CN (2) CN103249845B (pt)
AR (1) AR083309A1 (pt)
AU (2) AU2011312559B2 (pt)
BR (2) BR122020012639B8 (pt)
CA (1) CA2812576C (pt)
CL (2) CL2013000911A1 (pt)
ES (1) ES2744675T3 (pt)
HK (2) HK1219299A1 (pt)
HU (1) HUE045078T2 (pt)
MX (1) MX349350B (pt)
NZ (2) NZ608572A (pt)
RU (3) RU2747979C2 (pt)
UA (1) UA114886C2 (pt)
UY (1) UY33654A (pt)
WO (1) WO2012047595A2 (pt)
ZA (1) ZA201302204B (pt)

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CN111575398B (zh) * 2020-05-28 2022-05-03 北京市农林科学院 玉米第4号染色体雄性不育相关基因的分子标记及其应用
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ZA201302204B (en) 2014-05-28
EP2625291A4 (en) 2014-04-23
NZ707345A (en) 2017-01-27
AU2011312559B2 (en) 2016-05-19
EP2625291A2 (en) 2013-08-14
RU2603005C2 (ru) 2016-11-20
RU2747979C2 (ru) 2021-05-18
CN103249845B (zh) 2015-09-16
US10117411B2 (en) 2018-11-06
RU2016114532A (ru) 2018-11-28
ES2744675T3 (es) 2020-02-25
MX2013003917A (es) 2013-06-05
EP3018217B1 (en) 2019-06-05
BR112013008387B1 (pt) 2021-04-20
US20120090047A1 (en) 2012-04-12
EP3018217A3 (en) 2016-08-17
UA114886C2 (uk) 2017-08-28
WO2012047595A3 (en) 2012-08-16
RU2729107C2 (ru) 2020-08-04
AR083309A1 (es) 2013-02-13
CA2812576A1 (en) 2012-04-12
CL2013000911A1 (es) 2014-05-16
RU2016136834A (ru) 2018-12-11
RU2016114532A3 (pt) 2019-12-24
CL2016000546A1 (es) 2016-09-02
AU2011312559A1 (en) 2013-04-11
BR112013008387A2 (pt) 2016-06-14
RU2016136834A3 (pt) 2020-08-10
MX349350B (es) 2017-07-24
HK1219299A1 (zh) 2017-03-31
HUE045078T2 (hu) 2019-12-30
BR122020012639B8 (pt) 2022-10-11
AU2016216734B2 (en) 2018-01-25
HK1188258A1 (zh) 2014-04-25
RU2013119981A (ru) 2014-11-20
WO2012047595A2 (en) 2012-04-12
AU2016216734A1 (en) 2016-09-08
BR122020012639B1 (pt) 2021-04-27
NZ608572A (en) 2015-07-31
CN105177122A (zh) 2015-12-23
UY33654A (es) 2012-04-30
CN103249845A (zh) 2013-08-14
EP3018217A2 (en) 2016-05-11
CA2812576C (en) 2020-04-28

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