BR112021020608A8 - Rna recrutador de desaminase, construção, método para editar um rna alvo em uma célula hospedeira, e, construção, composição, célula, biblioteca ou kit - Google Patents

Rna recrutador de desaminase, construção, método para editar um rna alvo em uma célula hospedeira, e, construção, composição, célula, biblioteca ou kit

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Publication number
BR112021020608A8
BR112021020608A8 BR112021020608A BR112021020608A BR112021020608A8 BR 112021020608 A8 BR112021020608 A8 BR 112021020608A8 BR 112021020608 A BR112021020608 A BR 112021020608A BR 112021020608 A BR112021020608 A BR 112021020608A BR 112021020608 A8 BR112021020608 A8 BR 112021020608A8
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BR
Brazil
Prior art keywords
construction
rna
recruiter
deaminase
cell
Prior art date
Application number
BR112021020608A
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English (en)
Other versions
BR112021020608A2 (pt
Inventor
Yuan Pengfei
Zhao Yanxia
Liu Nengyin
Yi Zexuan
Tang Gangbin
Wei Wensheng
Qu Liang
Yi Zongyi
Zhu Shiyou
Wang Chunhui
Cao Zhongzheng
Zhou Zhuo
Original Assignee
Univ Beijing
Edigene Inc
Edigene Guangzhou Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Beijing, Edigene Inc, Edigene Guangzhou Inc filed Critical Univ Beijing
Publication of BR112021020608A2 publication Critical patent/BR112021020608A2/pt
Publication of BR112021020608A8 publication Critical patent/BR112021020608A8/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
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    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Compositions Of Macromolecular Compounds (AREA)

Abstract

RNA RECRUTADOR DE DESAMINASE, CONSTRUÇÃO, MÉTODO PARA EDITAR UM RNA ALVO EM UMA CÉLULA HOSPEDEIRA, E, CONSTRUÇÃO, COMPOSIÇÃO, CÉLULA, BIBLIOTECA OU KIT. São providos métodos para editar RNA, introduzindo um RNA recrutador de desaminase em uma célula hospedeira para desaminação de uma adenosina em um RNA alvo. São providos adicionalmente RNAs recrutadores de desaminase usados nos métodos para editar RNA e composições compreendendo os mesmos.
BR112021020608A 2019-04-15 2020-04-15 Rna recrutador de desaminase, construção, método para editar um rna alvo em uma célula hospedeira, e, construção, composição, célula, biblioteca ou kit BR112021020608A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019082713 2019-04-15
CN2019129952 2019-12-30
PCT/CN2020/084922 WO2020211780A1 (en) 2019-04-15 2020-04-15 Methods and compositions for editing rnas

Publications (2)

Publication Number Publication Date
BR112021020608A2 BR112021020608A2 (pt) 2022-02-22
BR112021020608A8 true BR112021020608A8 (pt) 2023-03-21

Family

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BR112021020608A BR112021020608A8 (pt) 2019-04-15 2020-04-15 Rna recrutador de desaminase, construção, método para editar um rna alvo em uma célula hospedeira, e, construção, composição, célula, biblioteca ou kit

Country Status (18)

Country Link
US (2) US20220307020A1 (pt)
EP (1) EP3956449A4 (pt)
JP (1) JP2022526455A (pt)
KR (1) KR20220004674A (pt)
CN (1) CN113939591A (pt)
AU (1) AU2020259548B2 (pt)
BR (1) BR112021020608A8 (pt)
CA (1) CA3136735A1 (pt)
CL (1) CL2021002695A1 (pt)
CO (1) CO2021015214A2 (pt)
CR (1) CR20210572A (pt)
EC (1) ECSP21080618A (pt)
IL (1) IL287248A (pt)
MX (1) MX2021012645A (pt)
PE (1) PE20212214A1 (pt)
SG (1) SG11202111401RA (pt)
TW (1) TW202043249A (pt)
WO (1) WO2020211780A1 (pt)

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MX2021012645A (es) 2021-11-12
CR20210572A (es) 2022-04-07
ECSP21080618A (es) 2021-12-30
US20220307020A1 (en) 2022-09-29
CO2021015214A2 (es) 2022-02-07
AU2020259548A1 (en) 2021-12-09
KR20220004674A (ko) 2022-01-11
SG11202111401RA (en) 2021-11-29
IL287248A (en) 2021-12-01
US20220098587A1 (en) 2022-03-31
CA3136735A1 (en) 2020-10-22
BR112021020608A2 (pt) 2022-02-22
EP3956449A1 (en) 2022-02-23
EP3956449A4 (en) 2023-03-29
AU2020259548B2 (en) 2023-10-12
CL2021002695A1 (es) 2022-07-01
PE20212214A1 (es) 2021-11-19
TW202043249A (zh) 2020-12-01
US11702658B2 (en) 2023-07-18
WO2020211780A1 (en) 2020-10-22
CN113939591A (zh) 2022-01-14
JP2022526455A (ja) 2022-05-24

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