BR112019011642A2 - sistema e métodos para o biocontrole de patógenos de planta - Google Patents

sistema e métodos para o biocontrole de patógenos de planta

Info

Publication number
BR112019011642A2
BR112019011642A2 BR112019011642A BR112019011642A BR112019011642A2 BR 112019011642 A2 BR112019011642 A2 BR 112019011642A2 BR 112019011642 A BR112019011642 A BR 112019011642A BR 112019011642 A BR112019011642 A BR 112019011642A BR 112019011642 A2 BR112019011642 A2 BR 112019011642A2
Authority
BR
Brazil
Prior art keywords
methods
pathogen
deliver
preferred
innovative
Prior art date
Application number
BR112019011642A
Other languages
English (en)
Inventor
Yin Karen
Costa Nunes Pedro
Sayre Richard
Original Assignee
Pebble Labs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pebble Labs Inc filed Critical Pebble Labs Inc
Publication of BR112019011642A2 publication Critical patent/BR112019011642A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H17/00—Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

Abstract

a tecnologia inventiva se refere a sistemas, métodos e estratégias inovadoras para o controle de patógenos de planta e herbívoros. a tecnologia inventiva pode incluir especificamente composições, sistemas e métodos inovadores configurados para entregar moléculas de rna inibitórias a uma planta infectada por patógeno. em uma modalidade preferencial, a invenção pode incluir bactérias endofíticas geneticamente modificadas configuradas para entregar uma ou mais moléculas de rna inibitórias a uma planta infectada por patógeno. uma modalidade preferencial pode incluir uma entrega trans-reino inovadora de rna grampo de cabelo que alveja proteínas virais codificadas resultando na redução de níveis de acúmulo de proteína viral.
BR112019011642A 2016-12-06 2017-12-06 sistema e métodos para o biocontrole de patógenos de planta BR112019011642A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662430671P 2016-12-06 2016-12-06
PCT/US2017/064977 WO2018106847A1 (en) 2016-12-06 2017-12-06 System and methods for the biocontrol of plant pathogens

Publications (1)

Publication Number Publication Date
BR112019011642A2 true BR112019011642A2 (pt) 2019-10-22

Family

ID=62491339

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019011642A BR112019011642A2 (pt) 2016-12-06 2017-12-06 sistema e métodos para o biocontrole de patógenos de planta

Country Status (11)

Country Link
US (1) US20200071713A1 (pt)
EP (1) EP3550960A4 (pt)
JP (1) JP2020511472A (pt)
KR (1) KR20190122647A (pt)
CN (1) CN110402085A (pt)
AU (1) AU2017370683A1 (pt)
BR (1) BR112019011642A2 (pt)
CA (1) CA3046081A1 (pt)
IL (1) IL267133A (pt)
MX (1) MX2019006510A (pt)
WO (1) WO2018106847A1 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210254058A1 (en) * 2018-02-22 2021-08-19 Dow Agrosciences Llc Short/small hairpin rna molecules
SG11202101777VA (en) * 2018-08-24 2021-03-30 Flagship Pioneering Innovations Vi Llc Methods and compositions for the modification of plants
CN109266580B (zh) * 2018-09-29 2022-06-14 新疆师范大学 一种共生抗干旱促种子萌发内生菌及其应用
CN109511587B (zh) * 2018-11-01 2021-03-30 浙江海洋大学 用于网箱孵化麦穗鱼的消毒剂
CN110241114B (zh) * 2019-03-20 2023-09-22 中国热带农业科学院热带生物技术研究所 一种可防控蚊虫的双链rna及其应用
CN110042115A (zh) * 2019-04-30 2019-07-23 贵州大学 一种马铃薯长管蚜效应蛋白Me23基因的重组载体和表达方法
CN110592101B (zh) * 2019-10-18 2020-09-25 河南大学 油菜转录因子BnWRKY184、克隆方法、载体、宿主细胞及应用
WO2021173953A1 (en) * 2020-02-26 2021-09-02 Pebble Labs, Inc. Novel plant endophytic bacteria and methods to control plant pathogens and pests
CN111771911B (zh) * 2020-06-30 2021-06-04 武汉合缘绿色生物股份有限公司 一种防治烟草花叶病毒的微生物制剂及其制备方法
CN112063623B (zh) * 2020-09-10 2022-03-25 南京农业大学 一种抑制水稻纹枯病菌的dsRNA及其应用
CN112458087B (zh) * 2020-11-06 2021-06-08 华南师范大学 基于美洲大蠊HDAC1基因设计的dsRNA、其制备方法、编码基因及其应用
WO2022117658A1 (en) * 2020-12-01 2022-06-09 Redbiotec Ag Bacterial delivery of gene silencing tools into eukaryotic cells
CN112940994B (zh) * 2021-04-25 2022-08-23 金禾佳农（北京）生物技术有限公司 一种枯草芽孢杆菌、培养方法及应用
CN113151281A (zh) * 2021-05-30 2021-07-23 中国农业大学 靶向沉默辣椒疫霉纤维素合酶3的植物表达载体及其应用
US20230115861A1 (en) * 2021-08-19 2023-04-13 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
CN114292316B (zh) * 2021-12-22 2023-03-24 西北农林科技大学 一种小麦条锈菌分泌蛋白、鉴定方法、转基因植株及应用
CN114806895B (zh) * 2022-05-20 2023-02-28 浙江大学 内生真菌m-b927在提高水稻谷粒产量中的应用
CN115058449B (zh) * 2022-06-24 2023-05-16 西南大学 一种利用CsWRKY43干扰以提高柑橘溃疡病抗性的方法
CN116064586B (zh) * 2022-11-01 2024-04-02 广东省农业科学院果树研究所 一种番木瓜CpWRKY50基因及其提高番木瓜炭疽病抗性的用途

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2402441A1 (en) * 2004-04-09 2012-01-04 Monsanto Technology, LLC Compositions and methods for control of insect infestations in plants
JP4249727B2 (ja) * 2005-05-13 2009-04-08 株式会社東芝 非水電解質電池およびリチウムチタン複合酸化物
AU2006329563B2 (en) * 2005-09-16 2014-02-20 Devgen Nv dsRNA as insect control agent
ES2568933T3 (es) * 2005-09-16 2016-05-05 Devgen N.V. ARNbc como agente de control de insectos
EP1963862A4 (en) * 2005-12-05 2010-03-03 Merck & Co Inc METHODS FOR PREDICTING REACTION TO TREATMENT FROM EXPRESSION PROFILES OF PROTEIN AND TRANSCRIPTIONAL BIOMARKERS
EP2347759B1 (en) * 2006-01-12 2017-10-18 deVGen N.V. Methods for controlling pests using RNAi
AU2007214547B2 (en) * 2006-02-13 2012-09-20 Monsanto Technology Llc Selecting and stabilizing dsRNA constructs
WO2008147816A2 (en) * 2007-05-22 2008-12-04 Cornell Research Foundation, Inc. Compositions and methods for the display of proteins on the surface of bacteria and their derived vesicles and uses thereof
US8901373B2 (en) * 2008-04-10 2014-12-02 Monsanto Technology Llc Methods and compositions for root knot nematode control
GB201009601D0 (en) * 2010-06-08 2010-07-21 Devgen Private Ltd Method for down-grading gene expression in fungi
BR112014031789A2 (pt) * 2012-06-22 2017-08-01 Syngenta Participations Ag controle biológico de pragas de coleópteros
BR122023020869A2 (pt) * 2014-09-17 2024-01-30 Spogen Biotech Inc Métodos para a remoção de exosporium a partir de esporos de uma bactéria de bacillus recombinante, para estimular o crescimento e proteger uma planta de um agente patogênico, bem como para aumentar resistência a stress em uma planta
WO2016096923A1 (en) * 2014-12-15 2016-06-23 Paris Sciences Et Lettres - Quartier Latin Methods to monitor post-transcriptional gene silencing activity in plant tissues/cell types relevant for pathogen entry, propagation or replication
ES2726674T3 (es) * 2014-12-23 2019-10-08 Syngenta Participations Ag Control biológico de plagas de coleópteros

Also Published As

Publication number Publication date
IL267133A (en) 2019-08-29
JP2020511472A (ja) 2020-04-16
CA3046081A1 (en) 2018-06-14
AU2017370683A1 (en) 2019-06-27
EP3550960A4 (en) 2020-08-12
WO2018106847A1 (en) 2018-06-14
KR20190122647A (ko) 2019-10-30
US20200071713A1 (en) 2020-03-05
CN110402085A (zh) 2019-11-01
MX2019006510A (es) 2020-08-03
EP3550960A1 (en) 2019-10-16

Similar Documents

Publication Publication Date Title
BR112019011642A2 (pt) sistema e métodos para o biocontrole de patógenos de planta
BR112018010805A2 (pt) mutantes de proteína f de rsv
CO2017012829A2 (es) Composiciones que comprenden cepas bacterianas
PH12017500614A1 (en) Compositions and methods for silencing hepatitis b virus gene expression
BR112019005183A2 (pt) sistema paratransgênico inovador para o biocontrole de mosquitos transmissores de doença
PE20180266A1 (es) Composiciones que comprenden cepas bacterianas
CY1120937T1 (el) Φαρμακευτικη μορφοποιηση αντισωματος anti-tnf-αλφα
WO2017019891A3 (en) Compositions and methods for silencing hepatitis b virus gene expression
WO2016183366A3 (en) Compositions and methods for silencing expression of hepatitis d virus rna
BR112013026929A2 (pt) composição e método para intensificação de uma resposta imune
CL2018002826A1 (es) Proteínas f de prefusión del virus respiratorio sincicial (vrs) solubles y estabilizadas
BR112018007622A2 (pt) ferramenta genética de transformação de bactérias clostridium
MX2020003841A (es) Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos.
BR112016003127A2 (pt) “composições e métodos para modular rna”
BR112016019267A2 (pt) métodos e composições para perfil de dna
EA201792509A1 (ru) Лечение рака
BR112017019625A2 (pt) udp-glicosiltransferases
BR112018070578A2 (pt) composições, sistemas, kits, e métodos para tratar uma infecção
BR112018073238A2 (pt) vírus oncolíticos compreendendo esrage e métodos de tratamento de câncer
BR112015030229A8 (pt) Partícula semelhante a vírus compreendendo um polipeptídeo e um antígeno da malária, vetor, composições farmacêutica e de vacina, bem como molécula de ácido nucleico isolado
EP4053146A3 (en) Molecule sensor systems
BR112018012304A2 (pt) composições e métodos para diminuir expressão de tau
WO2016183009A3 (en) Methods and compositions for the specific inhibition of antithrombin 3 (at3) by double-stranded rna
BR112017013453A2 (pt) novo peptídeo inibidor de pi3ky para o tratamento de doenças do sistema respiratório
PH12016502381A1 (en) Gene expression system

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2699 DE 27-09-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.