BR112019011378A2 - composições e métodos para a produção potencializada de enduracidina em uma cepa geneticamente modificada de streptomyces fungicidicus - Google Patents

composições e métodos para a produção potencializada de enduracidina em uma cepa geneticamente modificada de streptomyces fungicidicus

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Publication number
BR112019011378A2
BR112019011378A2 BR112019011378A BR112019011378A BR112019011378A2 BR 112019011378 A2 BR112019011378 A2 BR 112019011378A2 BR 112019011378 A BR112019011378 A BR 112019011378A BR 112019011378 A BR112019011378 A BR 112019011378A BR 112019011378 A2 BR112019011378 A2 BR 112019011378A2
Authority
BR
Brazil
Prior art keywords
enduracidin
production
methods
genetically modified
compositions
Prior art date
Application number
BR112019011378A
Other languages
English (en)
Inventor
T Zabriskie Mark
Yin Xihou
Original Assignee
Univ Oregon State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Oregon State filed Critical Univ Oregon State
Publication of BR112019011378A2 publication Critical patent/BR112019011378A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

esta invenção descreve composições e métodos para potencializar a produção de enduracidina em cepas geneticamente modificadas de streptomyces fungicidicus. em particular, a presente invenção descreve a manipulação genética de genes reguladores orf24 e orf18 associados ao cluster de genes da biossíntese de enduracidina (enramicina) de streptomyces fungicidicus para gerar constructos vetoriais e cepas recombinantes produzindo maiores rendimentos de enduracidina.
BR112019011378A 2016-12-06 2017-12-01 composições e métodos para a produção potencializada de enduracidina em uma cepa geneticamente modificada de streptomyces fungicidicus BR112019011378A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662430838P 2016-12-06 2016-12-06
US201762479087P 2017-03-30 2017-03-30
PCT/US2017/064328 WO2018106545A1 (en) 2016-12-06 2017-12-01 Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus

Publications (1)

Publication Number Publication Date
BR112019011378A2 true BR112019011378A2 (pt) 2019-10-15

Family

ID=60788701

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019011378A BR112019011378A2 (pt) 2016-12-06 2017-12-01 composições e métodos para a produção potencializada de enduracidina em uma cepa geneticamente modificada de streptomyces fungicidicus

Country Status (12)

Country Link
US (2) US11447530B2 (pt)
JP (1) JP7086984B2 (pt)
CN (1) CN110997700A (pt)
BR (1) BR112019011378A2 (pt)
CO (1) CO2019005761A2 (pt)
MX (2) MX2019006522A (pt)
MY (1) MY200048A (pt)
PE (1) PE20191767A1 (pt)
PH (1) PH12019501228A1 (pt)
TW (1) TWI828613B (pt)
UA (1) UA127452C2 (pt)
WO (1) WO2018106545A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113980881B (zh) * 2021-11-11 2023-09-19 枣庄市杰诺生物酶有限公司 一种高产恩拉霉素的杀真菌素链霉菌工程菌
CN115261258A (zh) * 2022-06-07 2022-11-01 四川汇邦环保科技有限公司 一种降解持久霉素的微生物菌剂

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1617881C2 (de) 1965-10-13 1984-07-05 Takeda Chemical Industries, Ltd., Osaka Antibiotikum Enduracidin und seine Herstellung
JPS6038120B2 (ja) 1980-10-31 1985-08-30 武田薬品工業株式会社 抗生物質エンジユラサイジンの製造法
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
WO1990001069A1 (en) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
JP3881009B2 (ja) 1994-07-15 2007-02-14 バイオメリオ・ベー・ベー 核酸増幅方法を改良するためのｒｎａポリメラーゼの使用
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US8188245B2 (en) 2006-09-29 2012-05-29 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University Enduracidin biosynthetic gene cluster from streptomyces fungicidicus
CN103374537B (zh) 2012-04-25 2015-01-07 牡丹江佰佳信生物科技有限公司 一种制备恩拉霉素的方法及其生产菌株
CN104131054B (zh) 2014-06-17 2017-02-01 安徽丰原发酵技术工程研究有限公司 一种用于提高恩拉霉素产量的发酵培养基及发酵方法
CN104561048A (zh) * 2015-01-23 2015-04-29 天津科技大学 杀真菌素链霉菌恩拉霉素抗性好且产量高的基因和菌株
BR112019011603A2 (pt) 2016-12-06 2020-08-18 Intervet International B.V. isolados de streptomyces fungicidicus modificados e seu uso

Also Published As

Publication number Publication date
US11447530B2 (en) 2022-09-20
US20230120672A1 (en) 2023-04-20
TW201827591A (zh) 2018-08-01
MY200048A (en) 2023-12-06
UA127452C2 (uk) 2023-08-30
MX2023003316A (es) 2023-03-27
CN110997700A (zh) 2020-04-10
CO2019005761A2 (es) 2019-08-30
WO2018106545A1 (en) 2018-06-14
RU2019120981A (ru) 2021-01-12
MX2019006522A (es) 2020-02-20
US20190359659A1 (en) 2019-11-28
JP7086984B2 (ja) 2022-06-20
PE20191767A1 (es) 2019-12-17
TWI828613B (zh) 2024-01-11
PH12019501228A1 (en) 2019-10-07
RU2019120981A3 (pt) 2021-08-23
JP2020500561A (ja) 2020-01-16
US11858967B2 (en) 2024-01-02

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]