CN115261258A - 一种降解持久霉素的微生物菌剂 - Google Patents
一种降解持久霉素的微生物菌剂 Download PDFInfo
- Publication number
- CN115261258A CN115261258A CN202210642070.1A CN202210642070A CN115261258A CN 115261258 A CN115261258 A CN 115261258A CN 202210642070 A CN202210642070 A CN 202210642070A CN 115261258 A CN115261258 A CN 115261258A
- Authority
- CN
- China
- Prior art keywords
- duramycin
- bacillus
- equal
- degrading
- gram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- SFWLDKQAUHFCBS-WWXQEMPQSA-N lancovutide Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H]2C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@@H](CCCCNC[C@H]4C(=O)N[C@@H](CC=5C=CC=CC=5)C(=O)NCC(=O)N5CCC[C@H]5C(=O)N[C@@H](CC=5C=CC=CC=5)C(=O)N[C@H]([C@@H](SC[C@H](NC(=O)[C@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CSC3C)CSC2)C(=O)N4)C)C(=O)N1)C(O)=O)[C@@H](O)C(O)=O)=O)C(C)C)C1=CC=CC=C1 SFWLDKQAUHFCBS-WWXQEMPQSA-N 0.000 title claims abstract description 27
- 108010067071 duramycin Proteins 0.000 title claims abstract description 26
- 230000000593 degrading effect Effects 0.000 title claims abstract description 20
- 230000000813 microbial effect Effects 0.000 title claims abstract description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 35
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 35
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 18
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 18
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 18
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims abstract description 18
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 18
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 18
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 17
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 17
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 15
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 15
- 230000015556 catabolic process Effects 0.000 claims abstract description 12
- 238000006731 degradation reaction Methods 0.000 claims abstract description 12
- 229940081969 saccharomyces cerevisiae Drugs 0.000 claims abstract description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 230000002085 persistent effect Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 241000235070 Saccharomyces Species 0.000 claims 1
- 239000002054 inoculum Substances 0.000 claims 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 230000007062 hydrolysis Effects 0.000 abstract description 5
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 239000001569 carbon dioxide Substances 0.000 abstract description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 3
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 abstract description 3
- 230000004048 modification Effects 0.000 abstract description 3
- 238000012986 modification Methods 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract description 3
- 235000019992 sake Nutrition 0.000 abstract description 3
- 230000028327 secretion Effects 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 8
- NJCUSQKMYNTYOW-MWUYRYRWSA-N enramicina Chemical compound O.N1C(=O)NC(=O)C(C=2C=C(Cl)C(O)=C(Cl)C=2)NC(=O)C(CO)NC(=O)C(C=2C=CC(O)=CC=2)NC(=O)C(CC2N=C(N)NC2)NC(=O)C(CCCNC(N)=O)NC(=O)C(C(C)O)NC(=O)C(C=2C=CC(O)=CC=2)NC(=O)C(C=2C=CC(O)=CC=2)NC(=O)C(C(C)O)NC(=O)N(CCCCN)C(=O)C(C=2C=CC(O)=CC=2)NC(=O)C(NC(=O)C(CC(O)=O)NC(=O)/C=C/C=C/CCCCC(C)CC)C(C)OC(=O)C(C=2C=CC(O)=CC=2)NC(=O)C(C)NC(=O)C1CC1CNC(N)=N1 NJCUSQKMYNTYOW-MWUYRYRWSA-N 0.000 description 5
- 108700041171 enramycin Proteins 0.000 description 5
- 229950003984 enramycin Drugs 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 150000002596 lactones Chemical group 0.000 description 2
- 238000001782 photodegradation Methods 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- MVTQIFVKRXBCHS-SMMNFGSLSA-N N-[(3S,6S,12R,15S,16R,19S,22S)-3-benzyl-12-ethyl-4,16-dimethyl-2,5,11,14,18,21,24-heptaoxo-19-phenyl-17-oxa-1,4,10,13,20-pentazatricyclo[20.4.0.06,10]hexacosan-15-yl]-3-hydroxypyridine-2-carboxamide (10R,11R,12E,17E,19E,21S)-21-hydroxy-11,19-dimethyl-10-propan-2-yl-9,26-dioxa-3,15,28-triazatricyclo[23.2.1.03,7]octacosa-1(27),6,12,17,19,25(28)-hexaene-2,8,14,23-tetrone Chemical compound CC(C)[C@H]1OC(=O)C2=CCCN2C(=O)c2coc(CC(=O)C[C@H](O)\C=C(/C)\C=C\CNC(=O)\C=C\[C@H]1C)n2.CC[C@H]1NC(=O)[C@@H](NC(=O)c2ncccc2O)[C@@H](C)OC(=O)[C@@H](NC(=O)[C@@H]2CC(=O)CCN2C(=O)[C@H](Cc2ccccc2)N(C)C(=O)[C@@H]2CCCN2C1=O)c1ccccc1 MVTQIFVKRXBCHS-SMMNFGSLSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 229940123317 Sulfonamide antibiotic Drugs 0.000 description 1
- 108010080702 Virginiamycin Proteins 0.000 description 1
- 239000004188 Virginiamycin Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 125000005313 fatty acid group Chemical group 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229960003842 virginiamycin Drugs 0.000 description 1
- 235000019373 virginiamycin Nutrition 0.000 description 1
- 150000003952 β-lactams Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Business, Economics & Management (AREA)
- General Chemical & Material Sciences (AREA)
- Emergency Management (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明涉及微生物菌剂技术领域，提供了一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉。本发明通过该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
Description
技术领域
本发明涉及微生物菌剂技术领域，具体涉及一种降解持久霉素的微生物菌剂。
背景技术
持久霉素(Enramycin)一种多肽类抗生素，又名恩霉素、安来霉素、恩拉霉素，是白色或微黄色粉末，分子量约为2500，融点为225～240℃，持久霉素易溶于稀盐酸、二甲基甲酰胺，可溶于甲醇、含水乙醇，难溶于丙酮，不溶于醋酸、苯、氯仿。持久霉素是一个巨大内酯环，由十二种氨基酸的十七个单体组成，脂肪酸侧链通过酰胺键与内酯环相连。
持久霉素是多肽类抗生素的一种，对葡萄球菌等革兰氏阳性菌有较强的抑菌能力，对其他抗生素产生耐药性的菌株仍有效，对支原体有较强抗菌力，因此这些硫肽霉素难以自动降解，会给周边环境和动植物的健康构成很大危害。
相关论文文献《多肽类抗生素的研究进展及其应用》第7页，查询网址为：https://www.docin.com/p-2028040684.html提到了通过蛋白酶来降解多肽类抗生素的方法，但这一方法目前尚不成熟。并且蛋白酶的提取工艺非常复杂、提取成本高，因此这种方法不适于大面积推广。
相关论文文献《抗生素在环境中降解的研究进展》第2-6页，查询网址为：https://wenku.so.com/d/5748e37b79e8f9a2332cce93289aedbd提到了通过水解、光降解、生物降解、微电解等四种方法来降解抗生素。其中水解方法多用于β-内酰胺类、大环内酯类和磺胺类抗生素；光降解方法多用于有吸收光子基团的抗生素；微电解多用于环状结构的抗生素；因此这些降解方法不适用于持久霉素。生物降解主要有植物降解和微生物降解两种方式，但是目前这两种方式在降解后得到的物质都存在着一定的污染性，无法实现绿色无害化降解。
发明内容
针对现有技术中的缺陷，本发明的目的是提供一种降解持久霉素的微生物菌剂，使其能够快速将养殖及农业产品及废弃物中的持久霉素残留降解。
为了实现上述目的，本发明通过如下的技术方案来实现：一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌(Bacillus amyloliquefaciens)、枯草芽孢杆菌(Bacillussubtilis)、凝结芽胞杆菌(Bacillus coagulans)、植物乳杆菌(lactobacillusplantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillus nige)。
进一步地，各组分菌粉的质量百分比为：解淀粉芽胞杆菌(Bacillusamyloliquefaciens)13％、枯草芽孢杆菌(Bacillus subtilis)9％、凝结芽胞杆菌(Bacillus coagulans)19％、植物乳杆菌(lactobacillus plantarum)17％、啤酒酵母菌(Beer yeast)14％、清酒酵母(Saka yeast)11％、保加利亚乳杆菌(Lactobacillusbulgaricus)9％、黑曲霉(Aspergillus nige)8％。
进一步地，各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillusamyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacillus coagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
为实现上述技术效果，本发明还提供了一种用于降解维吉尼亚霉素的微生物菌剂的应用，微生物菌剂应用于养殖、农业产品及废弃物中的持久霉素残留的降解。
本发明的有益效果：本发明提供的一种降解持久霉素的微生物菌剂，通过解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉几种特异性菌种组合，有效活菌的含量高，特别是针对性强，该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
具体实施方式
为使本发明的目的、技术方案和优点更加清楚明白，下面结合实施例，对本发明作进一步的详细说明，本发明的示意性实施方式及其说明仅用于解释本发明，并不作为对本发明的限定。
实施例1：
一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌(Bacillusamyloliquefaciens)、枯草芽孢杆菌(Bacillus subtilis)、凝结芽胞杆菌(Bacilluscoagulans)、植物乳杆菌(lactobacillus plantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillusnige)。
在本实施例中，通过解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉几种特异性菌种组合，有效活菌的含量高，特别是针对性强，该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
实施例2：
各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacilluscoagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
如上即为本发明的实施例。上述实施例以及实施例中的具体参数仅是为了清楚表述发明验证过程，并非用以限制本发明的专利保护范围，本发明的专利保护范围仍然以其权利要求书为准，凡是运用本发明的说明书内容所作的等同结构变化，同理均应包含在本发明的保护范围内。
Claims (4)
1.一种降解持久霉素的微生物菌剂，其特征在于：包括解淀粉芽胞杆菌(Bacillusamyloliquefaciens)、枯草芽孢杆菌(Bacillus subtilis)、凝结芽胞杆菌(Bacilluscoagulans)、植物乳杆菌(lactobacillus plantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillusnige)。
2.根据权利要求1所述的一种降解持久霉素的微生物菌剂，其特征在于：各组分菌粉的质量百分比为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)13％、枯草芽孢杆菌(Bacillus subtilis)9％、凝结芽胞杆菌(Bacillus coagulans)19％、植物乳杆菌(lactobacillus plantarum)17％、啤酒酵母菌(Beer yeast)14％、清酒酵母(Saka yeast)11％、保加利亚乳杆菌(Lactobacillus bulgaricus)9％、黑曲霉(Aspergillus nige)8％。
3.根据权利要求1所述的一种降解持久霉素的微生物菌剂，其特征在于：各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacillus coagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
4.根据权利要求1-4任意一项所述的降解持久霉素的微生物菌剂的应用，其特征在于：微生物菌剂应用于养殖、农业产品及废弃物中的持久霉素残留的降解。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210642070.1A CN115261258A (zh) | 2022-06-07 | 2022-06-07 | 一种降解持久霉素的微生物菌剂 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210642070.1A CN115261258A (zh) | 2022-06-07 | 2022-06-07 | 一种降解持久霉素的微生物菌剂 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115261258A true CN115261258A (zh) | 2022-11-01 |
Family
ID=83760207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210642070.1A Pending CN115261258A (zh) | 2022-06-07 | 2022-06-07 | 一种降解持久霉素的微生物菌剂 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115261258A (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434430A (zh) * | 2016-09-07 | 2017-02-22 | 中山市润泽生物科技有限公司 | 一种降解抗生素与农药残留的复合微生物菌剂及其制备与应用 |
| CN110072881A (zh) * | 2016-12-06 | 2019-07-30 | 英特维特国际股份有限公司 | 修饰的杀真菌素链霉菌分离物及其用途 |
| US20190359659A1 (en) * | 2016-12-06 | 2019-11-28 | Oregon State University | Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus |
| CN111286470A (zh) * | 2019-10-09 | 2020-06-16 | 天津科技大学 | 一株可降解红霉素的枯草芽孢杆菌及其应用 |
| CN114107128A (zh) * | 2021-12-15 | 2022-03-01 | 四川汇邦环保科技有限公司 | 一种用于降解硫酸黏杆菌素的微生物菌剂及其应用 |
| CN114214232A (zh) * | 2021-12-15 | 2022-03-22 | 四川汇邦环保科技有限公司 | 一种用于降解维吉尼亚霉素的微生物菌剂及其应用 |
-
2022
- 2022-06-07 CN CN202210642070.1A patent/CN115261258A/zh active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434430A (zh) * | 2016-09-07 | 2017-02-22 | 中山市润泽生物科技有限公司 | 一种降解抗生素与农药残留的复合微生物菌剂及其制备与应用 |
| CN110072881A (zh) * | 2016-12-06 | 2019-07-30 | 英特维特国际股份有限公司 | 修饰的杀真菌素链霉菌分离物及其用途 |
| US20190359659A1 (en) * | 2016-12-06 | 2019-11-28 | Oregon State University | Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus |
| CN111286470A (zh) * | 2019-10-09 | 2020-06-16 | 天津科技大学 | 一株可降解红霉素的枯草芽孢杆菌及其应用 |
| CN114107128A (zh) * | 2021-12-15 | 2022-03-01 | 四川汇邦环保科技有限公司 | 一种用于降解硫酸黏杆菌素的微生物菌剂及其应用 |
| CN114214232A (zh) * | 2021-12-15 | 2022-03-22 | 四川汇邦环保科技有限公司 | 一种用于降解维吉尼亚霉素的微生物菌剂及其应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9708634B2 (en) | Process for making chitin derivatives | |
| CN1840683A (zh) | 利用淀粉液化芽孢杆菌生产抗菌肽的方法及产品 | |
| CN102391950A (zh) | 一种餐厨垃圾除臭复合菌及其制备方法 | |
| Anand et al. | Digestion of cellulose and xylan by symbiotic bacteria in the intestine of the Indian flying fox (Pteropus giganteus) | |
| Xu et al. | Production of ε-poly-lysine by Streptomyces albulus PD-1 via solid-state fermentation | |
| CN102433261A (zh) | 一种餐厨垃圾除油复合菌及其制备方法 | |
| CN102187945A (zh) | 一种平衡动物胃肠道健康的添加剂 | |
| Bruorton et al. | Gut microflora of vervet and samango monkeys in relation to diet | |
| KR20110087469A (ko) | 부레옥잠 추출물에서 배양된 생균제를 주성분으로 포함하는 사료첨가제 | |
| Abirami et al. | Screening and identification of chitin degrading bacteria from shrimp shell waste dumping soil environment and its media optimization for chitinase enzyme production | |
| CN112501152A (zh) | 一种防治土传病的复合微生物菌剂及其制备方法 | |
| CN115261258A (zh) | 一种降解持久霉素的微生物菌剂 | |
| CN111620724A (zh) | 一种猪粪有机肥发酵菌剂及其施用方法 | |
| CN101063094A (zh) | 短短小芽孢杆菌tw及防治水稻稻瘟病的活菌制剂 | |
| Makarios-Laham et al. | Biodegradability of chitin-and chitosan-containing films in soil environment | |
| CN103305225A (zh) | 一种利用芽孢发酵废液制备土壤修复改良剂的方法 | |
| CN102731175A (zh) | 有机物料腐熟用微生物菌剂 | |
| WO2019160365A1 (ko) | 효모를 이용하여 이취가 개선된 발효 조성물의 제조방법 | |
| Wang et al. | Production of antifungal materials by bioconversion of shellfish chitin wastes fermented by Pseudomonas fluorescens K-188 | |
| CN114214232A (zh) | 一种用于降解维吉尼亚霉素的微生物菌剂及其应用 | |
| CN101045903A (zh) | 一种益生菌冻干工艺 | |
| CN114107128A (zh) | 一种用于降解硫酸黏杆菌素的微生物菌剂及其应用 | |
| CN1263824C (zh) | 沙化土壤生物活性保水改良剂及其制作方法 | |
| KR101649476B1 (ko) | 바실러스 아밀로리쿼파시엔스 knu-1 (kctc18343p) 균주 또는 이의 배양액을 포함하는 생육저해제 | |
| CN117247853A (zh) | 一种降解硫肽霉素的微生物菌剂 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20221101 |