CN115261258A - 一种降解持久霉素的微生物菌剂 - Google Patents
一种降解持久霉素的微生物菌剂 Download PDFInfo
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Abstract
本发明涉及微生物菌剂技术领域，提供了一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉。本发明通过该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
Description
技术领域
本发明涉及微生物菌剂技术领域，具体涉及一种降解持久霉素的微生物菌剂。
背景技术
持久霉素(Enramycin)一种多肽类抗生素，又名恩霉素、安来霉素、恩拉霉素，是白色或微黄色粉末，分子量约为2500，融点为225～240℃，持久霉素易溶于稀盐酸、二甲基甲酰胺，可溶于甲醇、含水乙醇，难溶于丙酮，不溶于醋酸、苯、氯仿。持久霉素是一个巨大内酯环，由十二种氨基酸的十七个单体组成，脂肪酸侧链通过酰胺键与内酯环相连。
持久霉素是多肽类抗生素的一种，对葡萄球菌等革兰氏阳性菌有较强的抑菌能力，对其他抗生素产生耐药性的菌株仍有效，对支原体有较强抗菌力，因此这些硫肽霉素难以自动降解，会给周边环境和动植物的健康构成很大危害。
相关论文文献《多肽类抗生素的研究进展及其应用》第7页，查询网址为：https://www.docin.com/p-2028040684.html提到了通过蛋白酶来降解多肽类抗生素的方法，但这一方法目前尚不成熟。并且蛋白酶的提取工艺非常复杂、提取成本高，因此这种方法不适于大面积推广。
相关论文文献《抗生素在环境中降解的研究进展》第2-6页，查询网址为：https://wenku.so.com/d/5748e37b79e8f9a2332cce93289aedbd提到了通过水解、光降解、生物降解、微电解等四种方法来降解抗生素。其中水解方法多用于β-内酰胺类、大环内酯类和磺胺类抗生素；光降解方法多用于有吸收光子基团的抗生素；微电解多用于环状结构的抗生素；因此这些降解方法不适用于持久霉素。生物降解主要有植物降解和微生物降解两种方式，但是目前这两种方式在降解后得到的物质都存在着一定的污染性，无法实现绿色无害化降解。
发明内容
针对现有技术中的缺陷，本发明的目的是提供一种降解持久霉素的微生物菌剂，使其能够快速将养殖及农业产品及废弃物中的持久霉素残留降解。
为了实现上述目的，本发明通过如下的技术方案来实现：一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌(Bacillus amyloliquefaciens)、枯草芽孢杆菌(Bacillussubtilis)、凝结芽胞杆菌(Bacillus coagulans)、植物乳杆菌(lactobacillusplantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillus nige)。
进一步地，各组分菌粉的质量百分比为：解淀粉芽胞杆菌(Bacillusamyloliquefaciens)13％、枯草芽孢杆菌(Bacillus subtilis)9％、凝结芽胞杆菌(Bacillus coagulans)19％、植物乳杆菌(lactobacillus plantarum)17％、啤酒酵母菌(Beer yeast)14％、清酒酵母(Saka yeast)11％、保加利亚乳杆菌(Lactobacillusbulgaricus)9％、黑曲霉(Aspergillus nige)8％。
进一步地，各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillusamyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacillus coagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
为实现上述技术效果，本发明还提供了一种用于降解维吉尼亚霉素的微生物菌剂的应用，微生物菌剂应用于养殖、农业产品及废弃物中的持久霉素残留的降解。
本发明的有益效果：本发明提供的一种降解持久霉素的微生物菌剂，通过解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉几种特异性菌种组合，有效活菌的含量高，特别是针对性强，该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
具体实施方式
为使本发明的目的、技术方案和优点更加清楚明白，下面结合实施例，对本发明作进一步的详细说明，本发明的示意性实施方式及其说明仅用于解释本发明，并不作为对本发明的限定。
实施例1：
一种降解持久霉素的微生物菌剂，包括解淀粉芽胞杆菌(Bacillusamyloliquefaciens)、枯草芽孢杆菌(Bacillus subtilis)、凝结芽胞杆菌(Bacilluscoagulans)、植物乳杆菌(lactobacillus plantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillusnige)。
在本实施例中，通过解淀粉芽胞杆菌、枯草芽孢杆菌、凝结芽胞杆菌、植物乳杆菌、啤酒酵母菌、清酒酵母、保加利亚乳杆菌和黑曲霉几种特异性菌种组合，有效活菌的含量高，特别是针对性强，该组菌种之间相互协同，通过分泌产生多种特异性的降解酶，这些酶类进一步通过直接或间接修饰及水解作用破坏持久霉素的分子结构而导致降解，最终使持久霉素分解为二氧化碳、水或其它无毒无害小分子的物质，从而快速将养殖、农业产品及废弃物中的持久霉素降解，为充分实现其绿色无害化和资源化奠定基础。
实施例2：
各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacilluscoagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
如上即为本发明的实施例。上述实施例以及实施例中的具体参数仅是为了清楚表述发明验证过程，并非用以限制本发明的专利保护范围，本发明的专利保护范围仍然以其权利要求书为准，凡是运用本发明的说明书内容所作的等同结构变化，同理均应包含在本发明的保护范围内。
Claims (4)
1.一种降解持久霉素的微生物菌剂，其特征在于：包括解淀粉芽胞杆菌(Bacillusamyloliquefaciens)、枯草芽孢杆菌(Bacillus subtilis)、凝结芽胞杆菌(Bacilluscoagulans)、植物乳杆菌(lactobacillus plantarum)、啤酒酵母菌(Beer yeast)、清酒酵母(Saka yeast)、保加利亚乳杆菌(Lactobacillus bulgaricus)和黑曲霉(Aspergillusnige)。
2.根据权利要求1所述的一种降解持久霉素的微生物菌剂，其特征在于：各组分菌粉的质量百分比为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)13％、枯草芽孢杆菌(Bacillus subtilis)9％、凝结芽胞杆菌(Bacillus coagulans)19％、植物乳杆菌(lactobacillus plantarum)17％、啤酒酵母菌(Beer yeast)14％、清酒酵母(Saka yeast)11％、保加利亚乳杆菌(Lactobacillus bulgaricus)9％、黑曲霉(Aspergillus nige)8％。
3.根据权利要求1所述的一种降解持久霉素的微生物菌剂，其特征在于：各组分菌种活细胞数量为：解淀粉芽胞杆菌(Bacillus amyloliquefaciens)≧2亿/克、枯草芽孢杆菌(Bacillus subtilis)≧1亿/克、凝结芽胞杆菌(Bacillus coagulans)≧4亿/克、植物乳杆菌(lactobacillus plantarum)≧3.5亿/克、啤酒酵母菌(Beer yeast)≧7亿/克、清酒酵母(Saka yeast)≧1.5亿/克、保加利亚乳杆菌(Lactobacillus bulgaricus)≧6亿/克、黑曲霉(Aspergillus nige)≧1.3亿/克。
4.根据权利要求1-4任意一项所述的降解持久霉素的微生物菌剂的应用，其特征在于：微生物菌剂应用于养殖、农业产品及废弃物中的持久霉素残留的降解。
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