BR112013027031A2 - transformação melhorada de monocotiledôneas recalcitrantes - Google Patents
transformação melhorada de monocotiledôneas recalcitrantesInfo
- Publication number
- BR112013027031A2 BR112013027031A2 BR112013027031A BR112013027031A BR112013027031A2 BR 112013027031 A2 BR112013027031 A2 BR 112013027031A2 BR 112013027031 A BR112013027031 A BR 112013027031A BR 112013027031 A BR112013027031 A BR 112013027031A BR 112013027031 A2 BR112013027031 A2 BR 112013027031A2
- Authority
- BR
- Brazil
- Prior art keywords
- recalcitrant
- monocotyledons
- improved
- improved transformation
- transformation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
- C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01008—Mannose-6-phosphate isomerase (5.3.1.8)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
resumo patente de invenção: "transformação melhorada de monocotiledôneas recalcitrantes". a presente invenção refere-se a métodos aqui que são melhorados para transformar plantas monocotiledôneas, assim como uma região codificando a proteína fosfomanose-isomerase (pmi) e vetores de transformação incluindo as mesmas.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161479131P | 2011-04-26 | 2011-04-26 | |
US61/479,131 | 2011-04-26 | ||
PCT/US2012/034982 WO2012149011A1 (en) | 2011-04-26 | 2012-04-25 | Enhanced transformation of recalcitrant monocots |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112013027031A2 true BR112013027031A2 (pt) | 2016-12-27 |
BR112013027031B1 BR112013027031B1 (pt) | 2021-02-02 |
Family
ID=47069075
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112013027031-4A BR112013027031B1 (pt) | 2011-04-26 | 2012-04-25 | método para aumentar a frequência de transformação de um tecido de plantas monocotiledôneas, vetor recombinante e ácido nucleico |
Country Status (7)
Country | Link |
---|---|
US (1) | US9121027B2 (pt) |
CN (1) | CN103620040A (pt) |
AU (1) | AU2012249818B2 (pt) |
BR (1) | BR112013027031B1 (pt) |
CA (1) | CA2829974C (pt) |
WO (1) | WO2012149011A1 (pt) |
ZA (1) | ZA201308023B (pt) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014200842A2 (en) * | 2013-06-11 | 2014-12-18 | Syngenta Participations Ag | Methods for generating transgenic plants |
CN105330742A (zh) * | 2014-08-08 | 2016-02-17 | 北京华大蛋白质研发中心有限公司 | 抗pmi蛋白的单克隆抗体及其应用 |
BR112019018059A2 (pt) | 2017-03-07 | 2020-08-04 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, método para produzir um polipeptídeo, método de controle de ervas daninhas, uso do ácido nucleico e produto de utilidade |
BR112019018175A2 (pt) | 2017-03-07 | 2020-04-07 | BASF Agricultural Solutions Seed US LLC | molécula, célula, planta, sementes, polipeptídeos recombinantes, método para produzir um polipeptídeo, planta, método para controlar ervas, uso do ácido nucleico e produto de utilidade |
CN110607323A (zh) * | 2019-09-24 | 2019-12-24 | 四川育良生物科技有限公司 | 一种农杆菌介导水稻遗传转化方法 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4940835A (en) | 1985-10-29 | 1990-07-10 | Monsanto Company | Glyphosate-resistant plants |
DE3687682T2 (de) | 1985-08-07 | 1993-08-19 | Monsanto Co | Glyphosat resistente pflanzen. |
US5994629A (en) * | 1991-08-28 | 1999-11-30 | Novartis Ag | Positive selection |
GB9304200D0 (en) | 1993-03-02 | 1993-04-21 | Sandoz Ltd | Improvements in or relating to organic compounds |
UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
US6037522A (en) | 1998-06-23 | 2000-03-14 | Rhone-Poulenc Agro | Agrobacterium-mediated transformation of monocots |
US6531648B1 (en) | 1998-12-17 | 2003-03-11 | Syngenta Participations Ag | Grain processing method and transgenic plants useful therein |
EP1311696B1 (en) | 2000-08-11 | 2012-07-25 | Syngenta Participations AG | Methods for stable transformation of plants |
AU2006225290B2 (en) * | 2001-08-27 | 2008-07-24 | Syngenta Participations Ag | Self-processing plants and plant parts |
WO2005086813A2 (en) * | 2004-03-08 | 2005-09-22 | Syngenta Participations Ag | Glutamine-rich maize seed protein and promoter |
US8076534B2 (en) * | 2005-07-22 | 2011-12-13 | Syngenta Participations Ag | Chlamydomonas glucan dikinase gene, enzyme and modified starch, uses, methods for production thereof |
US8404930B2 (en) * | 2009-01-26 | 2013-03-26 | Pioneer Hi-Bred International, Inc. | Methods for improving monocot transformation |
AU2010264445A1 (en) | 2009-06-25 | 2012-02-09 | Queensland University Of Technology | Methods for agrobacterium-mediated transformation of sugar cane |
-
2012
- 2012-04-25 AU AU2012249818A patent/AU2012249818B2/en active Active
- 2012-04-25 CN CN201280019351.6A patent/CN103620040A/zh active Pending
- 2012-04-25 BR BR112013027031-4A patent/BR112013027031B1/pt active IP Right Grant
- 2012-04-25 US US13/455,751 patent/US9121027B2/en active Active
- 2012-04-25 CA CA2829974A patent/CA2829974C/en active Active
- 2012-04-25 WO PCT/US2012/034982 patent/WO2012149011A1/en active Application Filing
-
2013
- 2013-10-29 ZA ZA2013/08023A patent/ZA201308023B/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN103620040A (zh) | 2014-03-05 |
US20120278950A1 (en) | 2012-11-01 |
AU2012249818B2 (en) | 2014-11-20 |
US9121027B2 (en) | 2015-09-01 |
AU2012249818A1 (en) | 2013-03-28 |
CA2829974C (en) | 2023-08-01 |
CA2829974A1 (en) | 2012-11-01 |
WO2012149011A1 (en) | 2012-11-01 |
ZA201308023B (en) | 2015-03-25 |
BR112013027031B1 (pt) | 2021-02-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 25/04/2012, OBSERVADAS AS CONDICOES LEGAIS. |