BR112018068890A2 - dna polimerases marinhas - Google Patents
dna polimerases marinhasInfo
- Publication number
- BR112018068890A2 BR112018068890A2 BR112018068890A BR112018068890A BR112018068890A2 BR 112018068890 A2 BR112018068890 A2 BR 112018068890A2 BR 112018068890 A BR112018068890 A BR 112018068890A BR 112018068890 A BR112018068890 A BR 112018068890A BR 112018068890 A2 BR112018068890 A2 BR 112018068890A2
- Authority
- BR
- Brazil
- Prior art keywords
- dna
- polymerases
- dna polymerase
- sequence
- present
- Prior art date
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 6
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 241000059282 Psychrobacillus sp. Species 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/101—Linear amplification, i.e. non exponential
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/119—Strand displacement amplification [SDA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
a presente invenção refere-se a dna polimerases. em particular, a presente invenção refere-se a dna polimerases baseadas em uma dna polimerase de um psicrobacillus sp. a presente invenção fornece uma dna polimerase isolada ou um fragmento enzimaticamente ativo da mesma, a referida dna polimerase compreendendo a sequência de aminoácidos da seq id no: 1 ou compreendendo uma sequência de aminoácidos que é pelo menos 70% idêntica à seq id no: 1. a invenção também fornece moléculas de ácido nucleico compreendendo uma sequências de nucleotídeos que codifica a dna polimerase. a invenção também fornece um método de polimerização de nucleotídeos e um método de amplificação de um ácido nucleico em que a dna polimerase ou um fragmento enzimaticamente ativo da mesma é usada(o).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1604876.1A GB201604876D0 (en) | 2016-03-22 | 2016-03-22 | Marine DNA polymerases |
| PCT/EP2017/056865 WO2017162765A1 (en) | 2016-03-22 | 2017-03-22 | Marine dna polymerases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112018068890A2 true BR112018068890A2 (pt) | 2019-01-22 |
Family
ID=55968721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112018068890A BR112018068890A2 (pt) | 2016-03-22 | 2017-03-22 | dna polimerases marinhas |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10787653B2 (pt) |
| EP (1) | EP3433357B1 (pt) |
| JP (1) | JP6986286B2 (pt) |
| KR (1) | KR102377213B1 (pt) |
| CN (1) | CN109072202B (pt) |
| BR (1) | BR112018068890A2 (pt) |
| CA (1) | CA3018433A1 (pt) |
| ES (1) | ES2917190T3 (pt) |
| GB (1) | GB201604876D0 (pt) |
| LT (1) | LT3433357T (pt) |
| PL (1) | PL3433357T3 (pt) |
| SG (1) | SG11201807651TA (pt) |
| WO (1) | WO2017162765A1 (pt) |
| ZA (1) | ZA201806872B (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201604876D0 (en) * | 2016-03-22 | 2016-05-04 | Uni I Tromsø Norges Arktiske Uni | Marine DNA polymerases |
| GB201721053D0 (en) | 2017-12-15 | 2018-01-31 | Univ I Tromsø - Norges Arktiske Univ | DNA polymerases |
| EP4041878A1 (en) | 2019-10-01 | 2022-08-17 | Universitetet i Tromsø - Norges Arktiske Universitet | Marine dna polymerase i |
| CA3155624A1 (en) | 2019-10-01 | 2021-04-08 | Universitetet I Tromso - Norges Arktiske Universitet | Dna polymerase and dna polymerase derived 3'-5'exonuclease |
| AU2022346850A1 (en) * | 2021-09-16 | 2024-03-28 | Cepheid | Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001136965A (ja) * | 1999-11-12 | 2001-05-22 | Takara Shuzo Co Ltd | 核酸配列の増幅方法 |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| AU2004203649B2 (en) * | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| EP2481815B1 (en) | 2006-05-11 | 2016-01-27 | Raindance Technologies, Inc. | Microfluidic devices |
| US8993298B1 (en) | 2012-08-31 | 2015-03-31 | New England Biolabs, Inc. | DNA polymerases |
| CA2888148C (en) * | 2012-12-13 | 2019-01-15 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| GB201604876D0 (en) * | 2016-03-22 | 2016-05-04 | Uni I Tromsø Norges Arktiske Uni | Marine DNA polymerases |
-
2016
- 2016-03-22 GB GBGB1604876.1A patent/GB201604876D0/en not_active Ceased
-
2017
- 2017-03-22 BR BR112018068890A patent/BR112018068890A2/pt active Search and Examination
- 2017-03-22 US US16/087,242 patent/US10787653B2/en active Active
- 2017-03-22 WO PCT/EP2017/056865 patent/WO2017162765A1/en active Application Filing
- 2017-03-22 ES ES17713609T patent/ES2917190T3/es active Active
- 2017-03-22 LT LTEPPCT/EP2017/056865T patent/LT3433357T/lt unknown
- 2017-03-22 SG SG11201807651TA patent/SG11201807651TA/en unknown
- 2017-03-22 JP JP2019501756A patent/JP6986286B2/ja active Active
- 2017-03-22 EP EP17713609.0A patent/EP3433357B1/en active Active
- 2017-03-22 PL PL17713609.0T patent/PL3433357T3/pl unknown
- 2017-03-22 CA CA3018433A patent/CA3018433A1/en active Pending
- 2017-03-22 KR KR1020187030155A patent/KR102377213B1/ko active IP Right Grant
- 2017-03-22 CN CN201780019082.6A patent/CN109072202B/zh active Active
-
2018
- 2018-10-15 ZA ZA2018/06872A patent/ZA201806872B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3433357A1 (en) | 2019-01-30 |
| JP2019509766A (ja) | 2019-04-11 |
| LT3433357T (lt) | 2022-06-27 |
| EP3433357B1 (en) | 2022-04-20 |
| ES2917190T3 (es) | 2022-07-07 |
| KR102377213B1 (ko) | 2022-03-22 |
| KR20180132711A (ko) | 2018-12-12 |
| WO2017162765A1 (en) | 2017-09-28 |
| CN109072202B (zh) | 2023-02-03 |
| GB201604876D0 (en) | 2016-05-04 |
| CN109072202A (zh) | 2018-12-21 |
| PL3433357T3 (pl) | 2022-10-24 |
| SG11201807651TA (en) | 2018-10-30 |
| ZA201806872B (en) | 2019-07-31 |
| CA3018433A1 (en) | 2017-09-28 |
| JP6986286B2 (ja) | 2021-12-22 |
| US20190106686A1 (en) | 2019-04-11 |
| US10787653B2 (en) | 2020-09-29 |
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Legal Events
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| B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
| B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] |