SG11201807651TA - Marine dna polymerases - Google Patents

Marine dna polymerases

Info

Publication number
SG11201807651TA
SG11201807651TA SG11201807651TA SG11201807651TA SG11201807651TA SG 11201807651T A SG11201807651T A SG 11201807651TA SG 11201807651T A SG11201807651T A SG 11201807651TA SG 11201807651T A SG11201807651T A SG 11201807651TA SG 11201807651T A SG11201807651T A SG 11201807651TA
Authority
SG
Singapore
Prior art keywords
tromso
international
dna polymerase
pct
dna polymerases
Prior art date
Application number
SG11201807651TA
Inventor
Atle Noralf Larsen
Yvonne Piotrowski
Netsanet Gizaw Assefa
Olav Lanes
Original Assignee
Univ I Tromsoe Norges Arktiske Univ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ I Tromsoe Norges Arktiske Univ filed Critical Univ I Tromsoe Norges Arktiske Univ
Publication of SG11201807651TA publication Critical patent/SG11201807651TA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/101—Linear amplification, i.e. non exponential
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/162765 Al 28 September 2017 (28.09.2017) WIPO I PCT (51) International Patent Classification: C12N 9/12 (2006.01) (21) International Application Number: PCT/EP2017/056865 (22) International Filing Date: 22 March 2017 (22.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1604876.1 22 March 2016 (22.03.2016) GB (71) Applicant: UNIVERSITETET I TROMSO - NORGES ARKTISKE UNIVERSITET [NO/NO]; PO Box 6050, Langnes, N-9037 Tromso (NO). (72) Inventors: LARSEN, Atle Noralf; Sykehusveien 23, 9019 Tromso (NO). PIOTROWSKI, Yvonne; Sykehusveien 23, 9019 Tromso (NO). ASSEFA, Netsanet Gizaw; Syke- husveien 23, 9019 Tromso (NO). LANES, Olav; Sykehus- veien 23, 9019 Tromso (NO). (74) Agent: GARDNER, Rebecca; DEHNS, St Bride's House, 10 Salisbury Square, London, Greater London EC4Y 8JD (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) = (54) Title: MARINE DNA POLYMERASES Figure 5 120 1rD Temperature eIC) (57) : The present invention relates to DNA polymerases. In particular, the present invention relates to DNA polymerases based on a DNA polymerase from a Psychrobacillus sp. The present invention provides an isolated DNA polymerase or an enzymat- ically active fragment thereof, said DNA polymerase comprising the amino acid sequence of SEQ ID NO:1 or comprising an amino acid sequence which is at least 70% identical to SEQ ID NO:l. The invention also provides nucleic acid molecules comprising a nucleotide sequence that encodes the DNA polymerase. The invention also provides a method of nucleotide polymerisation and a method of amplifying a nucleic acid in which the DNA polymerase or an enzymatically active fragment thereof is used. 111111111111110111011111111111010111110111011111111010111111111111111111111110111111 W O 20 17 / 16 27 65 Al
SG11201807651TA 2016-03-22 2017-03-22 Marine dna polymerases SG11201807651TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1604876.1A GB201604876D0 (en) 2016-03-22 2016-03-22 Marine DNA polymerases
PCT/EP2017/056865 WO2017162765A1 (en) 2016-03-22 2017-03-22 Marine dna polymerases

Publications (1)

Publication Number Publication Date
SG11201807651TA true SG11201807651TA (en) 2018-10-30

Family

ID=55968721

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201807651TA SG11201807651TA (en) 2016-03-22 2017-03-22 Marine dna polymerases

Country Status (14)

Country Link
US (1) US10787653B2 (en)
EP (1) EP3433357B1 (en)
JP (1) JP6986286B2 (en)
KR (1) KR102377213B1 (en)
CN (1) CN109072202B (en)
BR (1) BR112018068890A2 (en)
CA (1) CA3018433A1 (en)
ES (1) ES2917190T3 (en)
GB (1) GB201604876D0 (en)
LT (1) LT3433357T (en)
PL (1) PL3433357T3 (en)
SG (1) SG11201807651TA (en)
WO (1) WO2017162765A1 (en)
ZA (1) ZA201806872B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
GB201721053D0 (en) * 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
CA3155624A1 (en) 2019-10-01 2021-04-08 Universitetet I Tromso - Norges Arktiske Universitet Dna polymerase and dna polymerase derived 3'-5'exonuclease
US20230220449A1 (en) 2019-10-01 2023-07-13 Universitetet I Tromsø - Norges Arktiske Universitet Marine dna polymerase i
WO2023043951A2 (en) * 2021-09-16 2023-03-23 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001136965A (en) * 1999-11-12 2001-05-22 Takara Shuzo Co Ltd Method for amplifying nucleic acid sequence
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
AU2004203649B2 (en) * 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US8993298B1 (en) * 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
CN104854237A (en) * 2012-12-13 2015-08-19 霍夫曼-拉罗奇有限公司 Dna polymerases with improved activity
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases

Also Published As

Publication number Publication date
EP3433357B1 (en) 2022-04-20
JP6986286B2 (en) 2021-12-22
PL3433357T3 (en) 2022-10-24
EP3433357A1 (en) 2019-01-30
ES2917190T3 (en) 2022-07-07
ZA201806872B (en) 2019-07-31
BR112018068890A2 (en) 2019-01-22
US20190106686A1 (en) 2019-04-11
US10787653B2 (en) 2020-09-29
CA3018433A1 (en) 2017-09-28
CN109072202B (en) 2023-02-03
JP2019509766A (en) 2019-04-11
KR20180132711A (en) 2018-12-12
CN109072202A (en) 2018-12-21
KR102377213B1 (en) 2022-03-22
LT3433357T (en) 2022-06-27
WO2017162765A1 (en) 2017-09-28
GB201604876D0 (en) 2016-05-04

Similar Documents

Publication Publication Date Title
SG11201810179RA (en) Novel crispr enzymes and systems
SG11201807651TA (en) Marine dna polymerases
SG11201805805VA (en) Recombinogenic nucleic acid strands in situ
SG11201808721YA (en) Cell-free production of ribonucleic acid
SG11201808751SA (en) T cell receptors
SG11201810003UA (en) Using programmable dna binding proteins to enhance targeted genome modification
SG11201909918XA (en) Compositions and methods for improving sample identification in indexed nucleic acid libraries
SG11201809522WA (en) Method of nucleic acid sequence determination
SG11201808709VA (en) T cell receptors
SG11201808622SA (en) Chimeric receptors to flt3 and methods of use thereof
SG11201807252QA (en) Anti-lag-3 antibodies
SG11201809912UA (en) Hybrid carriers for nucleic acid cargo
SG11201810605UA (en) Selectively altering microbiota for immune modulation
SG11201808750PA (en) T cell receptors
SG11201909432SA (en) Compounds for increasing genome editing efficiency
SG11201806663TA (en) Closed-ended linear duplex dna for non-viral gene transfer
SG11201808552XA (en) T-cell modulatory multimeric polypeptides and methods of use thereof
SG11201803169WA (en) Cellulase variants and polynucleotides encoding same
SG11201810048VA (en) Tri-segmented pichinde viruses as vaccine vectors
SG11201809530PA (en) Method for recovery of phosphate
SG11201901371XA (en) Methods for the detection of genomic copy changes in dna samples
SG11201903456WA (en) Piperidine derivatives as inhibitors of ubiquitin specific protease 7
SG11201807636XA (en) Process for producing a polyacrylamide solution with increased viscosity
SG11201806398YA (en) Optimized factor viii genes
SG11201407505XA (en) Aptamer-based multiplexed assays