BR112017016791A2 - métodos e sistemas para validação de instrumento - Google Patents

métodos e sistemas para validação de instrumento

Info

Publication number
BR112017016791A2
BR112017016791A2 BR112017016791A BR112017016791A BR112017016791A2 BR 112017016791 A2 BR112017016791 A2 BR 112017016791A2 BR 112017016791 A BR112017016791 A BR 112017016791A BR 112017016791 A BR112017016791 A BR 112017016791A BR 112017016791 A2 BR112017016791 A2 BR 112017016791A2
Authority
BR
Brazil
Prior art keywords
amplification
values
amplification curves
fluorescence
systems
Prior art date
Application number
BR112017016791A
Other languages
English (en)
Other versions
BR112017016791B1 (pt
Inventor
Woo David
Freudenthal Jacob
Wessel Thomas
Chu Yong
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of BR112017016791A2 publication Critical patent/BR112017016791A2/pt
Publication of BR112017016791B1 publication Critical patent/BR112017016791B1/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Artificial Intelligence (AREA)
  • Public Health (AREA)
  • Biophysics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Data Mining & Analysis (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Evolutionary Computation (AREA)
  • Software Systems (AREA)
  • Bioethics (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mathematical Physics (AREA)
  • Signal Processing (AREA)
  • Molecular Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

é fornecido um método para validação de um instrumento. o método inclui receber dados de amplificação a partir de uma placa de validação para gerar uma pluralidade de curvas de amplificação (102, 202). a placa de validação inclui uma amostra de uma primeira quantidade e de uma segunda quantidade, e cada curva de amplificação inclui uma região exponencial. o método inclui ainda determinar um conjunto de limites de fluorescência com base nas regiões exponenciais da pluralidade de curvas de amplificação (104, 204) e determinar, para cada limite de fluorescência do conjunto, um primeiro conjunto de valores limite de ciclo (ct) de curvas de amplificação geradas a partir das amostras da primeira quantidade e um segundo conjunto de valores ct de curvas de amplificação gerados a partir das amostras da segunda quantidade (106, 206). o método inclui calcular se a primeira e a segunda quantidades são suficientemente discerníveis com base em valores ct em cada uma das pluralidades de limites de fluorescência (108, 208 a 218).
BR112017016791-3A 2015-02-06 2016-02-05 Método, sistema e mídia de armazenamento legível por computador para validar um instrumento BR112017016791B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562113077P 2015-02-06 2015-02-06
US62/113,077 2015-02-06
PCT/US2016/016730 WO2016127032A1 (en) 2015-02-06 2016-02-05 Methods and systems for instrument validation

Publications (2)

Publication Number Publication Date
BR112017016791A2 true BR112017016791A2 (pt) 2018-04-17
BR112017016791B1 BR112017016791B1 (pt) 2023-02-14

Family

ID=55411745

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017016791-3A BR112017016791B1 (pt) 2015-02-06 2016-02-05 Método, sistema e mídia de armazenamento legível por computador para validar um instrumento

Country Status (9)

Country Link
US (1) US10648912B2 (pt)
EP (1) EP3254080B1 (pt)
JP (1) JP6782244B2 (pt)
KR (1) KR102434235B1 (pt)
CN (1) CN107430066B (pt)
BR (1) BR112017016791B1 (pt)
RU (1) RU2017131048A (pt)
SG (1) SG11201706250VA (pt)
WO (1) WO2016127032A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019066238A2 (ko) * 2017-09-28 2019-04-04 주식회사 씨젠 증폭 데이터 표시 방법 및 장치
CN107622185B (zh) * 2017-10-27 2020-08-21 领航基因科技（杭州）有限公司 一种数字pcr浓度计算方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1485712A2 (en) * 2002-02-26 2004-12-15 Pharmacia Corporation Sequence detection system calculator
ES2357956T3 (es) * 2003-12-06 2011-05-04 Abbott Laboratories Método y sistema para analizar reacciones usando un sistema de información.
US7630849B2 (en) * 2005-09-01 2009-12-08 Applied Biosystems, Llc Method of automated calibration and diagnosis of laboratory instruments
EP2499262A4 (en) * 2009-11-12 2015-01-07 Esoterix Genetic Lab Llc ANALYSIS OF THE NUMBER OF COPIES OF GENETIC LOCUS
WO2011130184A2 (en) * 2010-04-11 2011-10-20 Life Technologies Corporation SYSTEMS AND METHODS FOR MODEL-BASED qPCR
WO2012068276A2 (en) * 2010-11-16 2012-05-24 Life Technologies Corporation Systems and methods for the analysis of proximity binding assay data
CN103688264B (zh) * 2011-02-01 2017-09-15 生命技术公司 用于蛋白熔解曲线数据分析的系统和方法
KR20120103496A (ko) * 2011-03-09 2012-09-19 삼성테크윈 주식회사 실시간 중합효소 연쇄 반응에 있어서 표준 곡선 및 임계값을 획득하는 방법
CN102787121B (zh) * 2012-06-14 2014-04-30 浙江大学 一种转录因子基因功能验证的方法
JP2014029282A (ja) * 2012-07-31 2014-02-13 Shimadzu Corp 分析装置バリデーションシステム及び該システム用プログラム
EP2976434B1 (en) 2013-03-18 2017-08-02 Life Technologies Corporation Methods and systems for analyzing biological reaction systems
CN103667439B (zh) * 2013-08-02 2015-05-13 福建医科大学附属第一医院 可区分hbv b型和非b型的双探针实时定量pcr法
CN104099370A (zh) * 2014-07-16 2014-10-15 中国人民解放军南京军区福州总医院 肿瘤靶向治疗的非整合慢病毒载体系统及制备方法和应用

Also Published As

Publication number Publication date
JP6782244B2 (ja) 2020-11-11
KR20170134361A (ko) 2017-12-06
CN107430066A (zh) 2017-12-01
EP3254080A1 (en) 2017-12-13
WO2016127032A1 (en) 2016-08-11
US10648912B2 (en) 2020-05-12
BR112017016791B1 (pt) 2023-02-14
US20160231245A1 (en) 2016-08-11
KR102434235B1 (ko) 2022-08-22
SG11201706250VA (en) 2017-08-30
CN107430066B (zh) 2021-07-13
EP3254080B1 (en) 2023-10-04
JP2018510619A (ja) 2018-04-19
RU2017131048A (ru) 2019-03-06

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 05/02/2016, OBSERVADAS AS CONDICOES LEGAIS