BR112016017398A2 - Material de proteína imobilizado, carreador, métodos para preparação de um material de proteína imobilizado e para catalisação de um reação, e, uso do material de proteína imobilizado - Google Patents

Material de proteína imobilizado, carreador, métodos para preparação de um material de proteína imobilizado e para catalisação de um reação, e, uso do material de proteína imobilizado

Info

Publication number
BR112016017398A2
BR112016017398A2 BR112016017398A BR112016017398A BR112016017398A2 BR 112016017398 A2 BR112016017398 A2 BR 112016017398A2 BR 112016017398 A BR112016017398 A BR 112016017398A BR 112016017398 A BR112016017398 A BR 112016017398A BR 112016017398 A2 BR112016017398 A2 BR 112016017398A2
Authority
BR
Brazil
Prior art keywords
immobilized protein
protein material
immobilized
catalyzing
preparing
Prior art date
Application number
BR112016017398A
Other languages
English (en)
Other versions
BR112016017398B1 (pt
Inventor
Engelmark CASSIMJEE Karim
Bäckvall Jan-Erling
Original Assignee
Enginzyme Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enginzyme Ab filed Critical Enginzyme Ab
Publication of BR112016017398A2 publication Critical patent/BR112016017398A2/pt
Publication of BR112016017398B1 publication Critical patent/BR112016017398B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

MATERIAL DE PROTEÍNA IMOBILIZADO, CARREADOR, MÉTODOS PARA PREPARAÇÃO DE UM MATERIAL DE PROTEÍNA IMOBILIZADO E PARA CATALISAÇÃO DE UM REAÇÃO, E, USO DO MATERIAL DE PROTEÍNA IMOBILIZADO. A invenção refere-se a um material de proteína imobilizado que compreende uma proteína que é imobilizada em um material vítreo ou polímero orgânico através da ligação por rótulo de afinidade. O material vítreo pode ser um material vítreo poroso, tal como vidro de porosidade controlada (híbrido). A invenção também refere-se ao uso de um material de enzima imobilizado como um biocatalisador heterogêneo na síntese química. A invenção ainda refere-se a um método para a imobilização de proteína rotuladas por afinidade em um material vítreo ou polímero orgânico e a um método para a purificação e isolamento de proteínas rotuladas por afinidade pela imobilização de tais proteínas em um material vítreo ou polímero orgânico.
BR112016017398-8A 2014-01-31 2015-01-30 Material de enzima imobilizado, uso de um carreador, e, métodos para preparação de um material de enzima imobilizado e para catalisação de uma reação BR112016017398B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE1450105-0 2014-01-31
SE1450105 2014-01-31
SE1450822 2014-07-02
SE1450822-0 2014-07-02
PCT/SE2015/050108 WO2015115993A1 (en) 2014-01-31 2015-01-30 Immobilized proteins and use thereof

Publications (2)

Publication Number Publication Date
BR112016017398A2 true BR112016017398A2 (pt) 2017-08-08
BR112016017398B1 BR112016017398B1 (pt) 2023-12-05

Family

ID=52649088

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016017398-8A BR112016017398B1 (pt) 2014-01-31 2015-01-30 Material de enzima imobilizado, uso de um carreador, e, métodos para preparação de um material de enzima imobilizado e para catalisação de uma reação

Country Status (17)

Country Link
US (3) US10793848B2 (pt)
EP (3) EP3910337B1 (pt)
JP (2) JP6534161B2 (pt)
KR (2) KR102465626B1 (pt)
CN (2) CN110951720B (pt)
AU (1) AU2015211437B2 (pt)
BR (1) BR112016017398B1 (pt)
CA (2) CA2936690C (pt)
CL (1) CL2016001921A1 (pt)
DK (3) DK3605100T3 (pt)
ES (3) ES2892319T3 (pt)
FI (1) FI3910337T3 (pt)
MX (2) MX2016009805A (pt)
PL (3) PL3910337T3 (pt)
RU (1) RU2684619C2 (pt)
WO (1) WO2015115993A1 (pt)
ZA (1) ZA201605760B (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10845276B2 (en) 2016-02-29 2020-11-24 Hewlett-Packard Development, L.P. Enzymatic sample purification
CN109022413A (zh) * 2018-08-10 2018-12-18 暨南大学 一种单胺氧化酶a微反应器及其制备方法和应用
KR20220035460A (ko) * 2019-07-17 2022-03-22 보너모스, 인코포레이티드 육탄당의 생산을 위한 고정화된 효소 조성물
CN111647561B (zh) * 2020-05-28 2022-12-20 大连理工大学 纳米抗体在细胞特异性捕获和细胞释放中的应用
GB202009513D0 (en) * 2020-06-22 2020-08-05 Univ Of Stellenbosch An enzyme-polymer matrix
CN113061158A (zh) * 2021-03-30 2021-07-02 南京工业大学 一种利用二价金属离子与组氨酸标签纯化并固定化蛋白的方法及其应用
CN113122445A (zh) * 2021-05-06 2021-07-16 南京普瑞特生物科技有限公司 固定化酶法拆分制备光学纯1-(1-萘基)乙胺的设备及使用方法
GB2614570A (en) 2022-01-10 2023-07-12 Uab Biomatter Designs C-nucleoside monophosphate synthesis
WO2023229519A1 (en) 2022-05-27 2023-11-30 Bunge Sa Batch process for enzymatic modification of lipids
WO2023227795A1 (en) 2022-05-27 2023-11-30 Enginzyme Ab Biocatalysts for organic synthesis

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56115727A (en) * 1980-02-19 1981-09-11 Kuraray Co Ltd Carrier for immobilizing physiologically active substance
US4632904A (en) * 1983-12-27 1986-12-30 Ciba Corning Diagnostics Corp. Immobilized enzyme composites having carriers derivatized with an organotitanate
US5610274A (en) * 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US6087452A (en) * 1998-06-02 2000-07-11 University Of Utah Metal-chelating surfacant
KR100506646B1 (ko) 2002-07-22 2005-08-03 현대모비스 주식회사 차량 라디에이터 상부 마운팅 구조
US20040115777A1 (en) * 2002-10-15 2004-06-17 Syngenta Participations Ag Identification of protein interactions using in vivo post-translationally modified fusion proteins
PL2170776T3 (pl) * 2007-06-28 2019-07-31 Prime Synthesis, Inc. Sposób wytwarzania powleczonej polimerem szklanej cząstki o regulowanej porowatości
EP2023140A1 (en) * 2007-08-08 2009-02-11 FUJIFILM Corporation Biosensor chip
JP2010190891A (ja) * 2009-01-22 2010-09-02 Fujifilm Corp 担体およびその製造方法並びに抽出操作器具
CN102260662B (zh) 2010-05-24 2017-04-05 中国科学院过程工程研究所 用于固定化酶的载体及其用途和固定有酶的载体
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
CN102998444B (zh) * 2012-07-15 2014-08-06 潍坊医学院 IgG抗体在聚苯乙烯载体表面的立体定向固定方法

Also Published As

Publication number Publication date
JP2017504350A (ja) 2017-02-09
PL3100050T3 (pl) 2020-01-31
AU2015211437A1 (en) 2016-07-21
CN110951720A (zh) 2020-04-03
EP3605100B1 (en) 2021-07-21
CA2936690C (en) 2023-09-05
EP3910337B1 (en) 2023-08-09
AU2015211437B2 (en) 2020-07-23
US20190241884A1 (en) 2019-08-08
WO2015115993A1 (en) 2015-08-06
KR102414887B1 (ko) 2022-07-01
PL3605100T3 (pl) 2021-12-13
PL3910337T3 (pl) 2024-01-29
MX2016009805A (es) 2016-10-28
FI3910337T3 (fi) 2023-10-03
DK3605100T3 (da) 2021-10-04
KR102465626B1 (ko) 2022-11-10
DK3100050T3 (da) 2019-11-04
RU2016134892A (ru) 2018-03-12
ES2958266T3 (es) 2024-02-06
JP6534161B2 (ja) 2019-06-26
ZA201605760B (en) 2019-11-27
EP3100050B1 (en) 2019-08-28
CA2936690A1 (en) 2015-08-06
CN110951720B (zh) 2023-08-18
RU2016134892A3 (pt) 2018-09-26
BR112016017398B1 (pt) 2023-12-05
DK3910337T3 (da) 2023-10-09
MX2022001420A (es) 2022-03-29
US20210139881A1 (en) 2021-05-13
CN105940104B (zh) 2020-01-10
US10793848B2 (en) 2020-10-06
EP3605100A1 (en) 2020-02-05
JP2019162133A (ja) 2019-09-26
CA3205514A1 (en) 2015-08-06
KR20160113705A (ko) 2016-09-30
CN105940104A (zh) 2016-09-14
CL2016001921A1 (es) 2017-10-06
KR20220098259A (ko) 2022-07-11
ES2892319T3 (es) 2022-02-03
EP3100050A1 (en) 2016-12-07
RU2684619C2 (ru) 2019-04-10
JP6908653B2 (ja) 2021-07-28
US20160340667A1 (en) 2016-11-24
ES2756601T3 (es) 2020-04-27
EP3910337A1 (en) 2021-11-17

Similar Documents

Publication Publication Date Title
BR112016017398A2 (pt) Material de proteína imobilizado, carreador, métodos para preparação de um material de proteína imobilizado e para catalisação de um reação, e, uso do material de proteína imobilizado
EP4219745A3 (en) Spatially encoded biological assays using a microfluidic device
BR112015009774A2 (pt) polipeptídeos citocromo p450 e citocromo p450 redutase, codificando moléculas de ácido nucléicos e usos das mesmas
GB201020995D0 (en) Biological materials and uses thereof
BR112016003035A2 (pt) método para preparação de rebaudiosídeo m através do uso de método enzimático
BR112016006285A2 (pt) método implementado usando um sistema de computador, produto de programa de computador, e, sistema
NZ731116A (en) Method for the enrichment of circulating tumor dna
MY169244A (en) Process for the preparation of a zeolitic material
BR112015019879A2 (pt) Composições de espécies de variantes moduladas de lisina e métodos para produção e uso das mesmas
WO2011158037A3 (en) Methods for direct pcr using a polymeric material as sample carrier
BR112014007798A2 (pt) implementação de comunicações seguras em um sistema de suporte
CO6480963A2 (es) Aptamero para quimasa y su uso
BR112018010229A2 (pt) sistema catalisador e processo para a produção de glicóis
CO7240396A2 (es) Purificación de iduronato-2-sulfatasa
WO2011109326A3 (en) Crosslinking reagents, methods, and compositions for studying protein-protein interactions
CL2013001621A1 (es) Molecula de acido nucleico reguladora con especificidad definida, construccion de expresion que la comprende, vector; microorganismo, celula vegetal o animal; y metodo para la produccion de dicha molecula.
BR112017028040A2 (pt) métodos e aparelhos para condicionamento de populações de células para terapias celulares
CL2014001338A1 (es) Método para la preparación de hidrogeles de alginato usando hidrolasas, particularmente lipasas; proceso que involucra insertar material biológico, como espermatozoides, en el hidrogel de alginato; hidrogel de alginato así preparado; y kit para la preparación.
GB2542576A (en) Method and kit of detecting the absence of micro-oranisms
BR112018010230A2 (pt) sistema catalisador e processo para a produção de glicóis
FR2958655B1 (fr) Procede de purification des extraits aromatiques contenant des composes polycycliques aromatiques
IN2015DN03035A (pt)
BR112017008897A2 (pt) métodos para produzir combustíveis, aditivos de gasolina, e lubrificantes usando catalisadores de amina
AR070905A1 (es) Procedimiento para la produccion de hidroperoxidos de alquilbenceno bajo condiciones suaves y en presencia de sistemas cataliticos
BR112015031531A2 (pt) produção de solvente

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B154 Notification of filing of divisional application [chapter 15.50 patent gazette]

Free format text: O PEDIDO FOI DIVIDIDO NO BR122023016794-5 PROTOCOLO 870230074001 EM 21/08/2023 16:30.

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 30/01/2015, OBSERVADAS AS CONDICOES LEGAIS