MX2022001420A - Proteinas inmovilizadas y uso de las mismas. - Google Patents

Proteinas inmovilizadas y uso de las mismas.

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Publication number
MX2022001420A
MX2022001420A MX2022001420A MX2022001420A MX2022001420A MX 2022001420 A MX2022001420 A MX 2022001420A MX 2022001420 A MX2022001420 A MX 2022001420A MX 2022001420 A MX2022001420 A MX 2022001420A MX 2022001420 A MX2022001420 A MX 2022001420A
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MX
Mexico
Prior art keywords
glass material
organic polymer
immobilized
relates
proteins
Prior art date
Application number
MX2022001420A
Other languages
English (en)
Inventor
Karim Engelmark Cassimjee
Jan - Erling BÄCKVALL
Original Assignee
Enginzyme Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enginzyme Ab filed Critical Enginzyme Ab
Publication of MX2022001420A publication Critical patent/MX2022001420A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
    • B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La invención se refiere a un material de proteína inmovilizada que comprende una proteína que se inmoviliza sobre un material de vidrio o polímero orgánico a través de ligación de etiqueta de afinidad. El material de vidrio puede ser un material de vidrio poroso tal como vidrio de porosidad controlada (híbrido). La invención también se refiere al uso de un material de enzima inmovilizada como un biocatalizador heterogéneo en la síntesis química. La invención se refiere adicionalmente a un método para la inmovilización de proteínas etiquetadas por afinidad sobre un material de vidrio o polímero orgánico, y a un método para la purificación y aislamiento de proteínas etiquetadas por afinidad mediante la inmovilización de tales proteínas sobre un material de vidrio o polímero orgánico.
MX2022001420A 2014-01-31 2016-07-27 Proteinas inmovilizadas y uso de las mismas. MX2022001420A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE1450105 2014-01-31
SE1450822 2014-07-02

Publications (1)

Publication Number Publication Date
MX2022001420A true MX2022001420A (es) 2022-03-29

Family

ID=52649088

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016009805A MX2016009805A (es) 2014-01-31 2015-01-30 Proteinas inmovilizadas y uso de las mismas.
MX2022001420A MX2022001420A (es) 2014-01-31 2016-07-27 Proteinas inmovilizadas y uso de las mismas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2016009805A MX2016009805A (es) 2014-01-31 2015-01-30 Proteinas inmovilizadas y uso de las mismas.

Country Status (17)

Country Link
US (3) US10793848B2 (es)
EP (3) EP3910337B1 (es)
JP (2) JP6534161B2 (es)
KR (2) KR102465626B1 (es)
CN (2) CN110951720B (es)
AU (1) AU2015211437B2 (es)
BR (1) BR112016017398B1 (es)
CA (2) CA2936690C (es)
CL (1) CL2016001921A1 (es)
DK (3) DK3605100T3 (es)
ES (3) ES2892319T3 (es)
FI (1) FI3910337T3 (es)
MX (2) MX2016009805A (es)
PL (3) PL3910337T3 (es)
RU (1) RU2684619C2 (es)
WO (1) WO2015115993A1 (es)
ZA (1) ZA201605760B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10845276B2 (en) 2016-02-29 2020-11-24 Hewlett-Packard Development, L.P. Enzymatic sample purification
CN109022413A (zh) * 2018-08-10 2018-12-18 暨南大学 一种单胺氧化酶a微反应器及其制备方法和应用
KR20220035460A (ko) * 2019-07-17 2022-03-22 보너모스, 인코포레이티드 육탄당의 생산을 위한 고정화된 효소 조성물
CN111647561B (zh) * 2020-05-28 2022-12-20 大连理工大学 纳米抗体在细胞特异性捕获和细胞释放中的应用
GB202009513D0 (en) * 2020-06-22 2020-08-05 Univ Of Stellenbosch An enzyme-polymer matrix
CN113061158A (zh) * 2021-03-30 2021-07-02 南京工业大学 一种利用二价金属离子与组氨酸标签纯化并固定化蛋白的方法及其应用
CN113122445A (zh) * 2021-05-06 2021-07-16 南京普瑞特生物科技有限公司 固定化酶法拆分制备光学纯1-(1-萘基)乙胺的设备及使用方法
GB2614570A (en) 2022-01-10 2023-07-12 Uab Biomatter Designs C-nucleoside monophosphate synthesis
WO2023229519A1 (en) 2022-05-27 2023-11-30 Bunge Sa Batch process for enzymatic modification of lipids
WO2023227795A1 (en) 2022-05-27 2023-11-30 Enginzyme Ab Biocatalysts for organic synthesis

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JPS56115727A (en) * 1980-02-19 1981-09-11 Kuraray Co Ltd Carrier for immobilizing physiologically active substance
US4632904A (en) * 1983-12-27 1986-12-30 Ciba Corning Diagnostics Corp. Immobilized enzyme composites having carriers derivatized with an organotitanate
US5610274A (en) * 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US6087452A (en) * 1998-06-02 2000-07-11 University Of Utah Metal-chelating surfacant
KR100506646B1 (ko) 2002-07-22 2005-08-03 현대모비스 주식회사 차량 라디에이터 상부 마운팅 구조
US20040115777A1 (en) * 2002-10-15 2004-06-17 Syngenta Participations Ag Identification of protein interactions using in vivo post-translationally modified fusion proteins
PL2170776T3 (pl) * 2007-06-28 2019-07-31 Prime Synthesis, Inc. Sposób wytwarzania powleczonej polimerem szklanej cząstki o regulowanej porowatości
EP2023140A1 (en) * 2007-08-08 2009-02-11 FUJIFILM Corporation Biosensor chip
JP2010190891A (ja) * 2009-01-22 2010-09-02 Fujifilm Corp 担体およびその製造方法並びに抽出操作器具
CN102260662B (zh) 2010-05-24 2017-04-05 中国科学院过程工程研究所 用于固定化酶的载体及其用途和固定有酶的载体
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CN102998444B (zh) * 2012-07-15 2014-08-06 潍坊医学院 IgG抗体在聚苯乙烯载体表面的立体定向固定方法

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JP2017504350A (ja) 2017-02-09
PL3100050T3 (pl) 2020-01-31
BR112016017398A2 (pt) 2017-08-08
AU2015211437A1 (en) 2016-07-21
CN110951720A (zh) 2020-04-03
EP3605100B1 (en) 2021-07-21
CA2936690C (en) 2023-09-05
EP3910337B1 (en) 2023-08-09
AU2015211437B2 (en) 2020-07-23
US20190241884A1 (en) 2019-08-08
WO2015115993A1 (en) 2015-08-06
KR102414887B1 (ko) 2022-07-01
PL3605100T3 (pl) 2021-12-13
PL3910337T3 (pl) 2024-01-29
MX2016009805A (es) 2016-10-28
FI3910337T3 (fi) 2023-10-03
DK3605100T3 (da) 2021-10-04
KR102465626B1 (ko) 2022-11-10
DK3100050T3 (da) 2019-11-04
RU2016134892A (ru) 2018-03-12
ES2958266T3 (es) 2024-02-06
JP6534161B2 (ja) 2019-06-26
ZA201605760B (en) 2019-11-27
EP3100050B1 (en) 2019-08-28
CA2936690A1 (en) 2015-08-06
CN110951720B (zh) 2023-08-18
RU2016134892A3 (es) 2018-09-26
BR112016017398B1 (pt) 2023-12-05
DK3910337T3 (da) 2023-10-09
US20210139881A1 (en) 2021-05-13
CN105940104B (zh) 2020-01-10
US10793848B2 (en) 2020-10-06
EP3605100A1 (en) 2020-02-05
JP2019162133A (ja) 2019-09-26
CA3205514A1 (en) 2015-08-06
KR20160113705A (ko) 2016-09-30
CN105940104A (zh) 2016-09-14
CL2016001921A1 (es) 2017-10-06
KR20220098259A (ko) 2022-07-11
ES2892319T3 (es) 2022-02-03
EP3100050A1 (en) 2016-12-07
RU2684619C2 (ru) 2019-04-10
JP6908653B2 (ja) 2021-07-28
US20160340667A1 (en) 2016-11-24
ES2756601T3 (es) 2020-04-27
EP3910337A1 (en) 2021-11-17

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