BR112016006285A2 - método implementado usando um sistema de computador, produto de programa de computador, e, sistema - Google Patents

método implementado usando um sistema de computador, produto de programa de computador, e, sistema

Info

Publication number
BR112016006285A2
BR112016006285A2 BR112016006285A BR112016006285A BR112016006285A2 BR 112016006285 A2 BR112016006285 A2 BR 112016006285A2 BR 112016006285 A BR112016006285 A BR 112016006285A BR 112016006285 A BR112016006285 A BR 112016006285A BR 112016006285 A2 BR112016006285 A2 BR 112016006285A2
Authority
BR
Brazil
Prior art keywords
methods
computer program
computer
program product
screening
Prior art date
Application number
BR112016006285A
Other languages
English (en)
Other versions
BR112016006285B1 (pt
Inventor
Scott Baskerville Donald
W Huisman Gjait
Javiniar Sarmiento Russell
Zhang Xiyun
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of BR112016006285A2 publication Critical patent/BR112016006285A2/pt
Publication of BR112016006285B1 publication Critical patent/BR112016006285B1/pt

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C99/00—Subject matter not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/20—Screening of libraries
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60—In silico combinatorial chemistry
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60—In silico combinatorial chemistry
    • G16C20/64—Screening of libraries

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Library & Information Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Computing Systems (AREA)
  • Ecology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

são descritos métodos para identificar biomoléculas com propriedades desejadas (ou que são a maioria adequadas para uma rodada de evolução direcionada) a partir de bibliotecas de biomolécula complexas ou conjuntos de tais bibliotecas. algumas modalidades da presente descrição provêm métodos para virtualmente triar proteínas para propriedades benéficas. algumas modalidades da presente descrição provêm métodos para virtualmente triar enzimas para atividade desejada e/ou seletividade para reações catalíticas que envolvem substratos particulares. algumas modalidades combinam triagem e evolução direcionada para projetar e desenvolver proteínas e enzimas tendo propriedades desejadas. sistemas e produtos de programa de computador que implementam os métodos também são providos.
BR112016006285-0A 2013-09-27 2014-09-26 Método implementado usando um sistema de computador, meio de armazenamento legível por computador, e, sistema BR112016006285B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361883838P 2013-09-27 2013-09-27
US61/883,838 2013-09-27
PCT/US2014/057899 WO2015048572A1 (en) 2013-09-27 2014-09-26 Automated screening of enzyme variants

Publications (2)

Publication Number Publication Date
BR112016006285A2 true BR112016006285A2 (pt) 2017-08-01
BR112016006285B1 BR112016006285B1 (pt) 2022-09-06

Family

ID=51662390

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016006285-0A BR112016006285B1 (pt) 2013-09-27 2014-09-26 Método implementado usando um sistema de computador, meio de armazenamento legível por computador, e, sistema

Country Status (15)

Country Link
US (3) US10696964B2 (pt)
EP (2) EP3418929B1 (pt)
JP (3) JP6857029B2 (pt)
KR (1) KR102342205B1 (pt)
CN (1) CN105765592B (pt)
AU (1) AU2014324669B2 (pt)
BR (1) BR112016006285B1 (pt)
CA (1) CA2923755C (pt)
DK (2) DK3049973T3 (pt)
ES (2) ES2693150T3 (pt)
HU (2) HUE053049T2 (pt)
IL (1) IL244457B (pt)
RU (2) RU2019140645A (pt)
SG (1) SG11201601695WA (pt)
WO (1) WO2015048572A1 (pt)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102341026B1 (ko) 2013-09-27 2021-12-21 코덱시스, 인코포레이티드 구조에 기반한 예측 모델링
KR102342205B1 (ko) 2013-09-27 2021-12-21 코덱시스, 인코포레이티드 효소 변이체의 자동화 스크리닝
WO2015073971A1 (en) * 2013-11-15 2015-05-21 InfiniteBio Computer-assisted modeling for treatment design
CA2968275C (en) 2014-11-25 2023-09-12 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
PL3237621T3 (pl) 2014-12-22 2023-10-30 Codexis, Inc. Warianty ludzkiej alfa-galaktozydazy
US10542961B2 (en) 2015-06-15 2020-01-28 The Research Foundation For The State University Of New York System and method for infrasonic cardiac monitoring
WO2017155945A1 (en) * 2016-03-09 2017-09-14 President And Fellows Of Harvard College Methods and systems of cell-free enzyme discovery and optimization
SG11201906880VA (en) 2017-02-13 2019-08-27 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
JP6917050B2 (ja) * 2017-03-06 2021-08-11 学校法人早稲田大学 最適特性を有する非天然型タンパク質の製造方法
US11021729B2 (en) 2017-04-27 2021-06-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
CA3062550A1 (en) * 2017-05-08 2018-11-15 Codexis, Inc. Engineered ligase variants
EP3404567A1 (en) * 2017-05-19 2018-11-21 Fujitsu Limited A system and a method for discovery of predicted site-specific protein phosphorylation candidates
EP3642748A4 (en) * 2017-06-19 2021-03-10 Jungla LLC INTERPRETATION OF GENETIC AND GENOMIC VARIANTS VIA AN INTEGRATED COMPUTING AND EXPERIMENTAL FRAMEWORK FOR DEEP MUTATION LEARNING
RU2020103727A (ru) 2017-06-30 2021-07-30 Кодексис, Инк. Варианты рнк-полимеразы т7
EP3645711A4 (en) 2017-06-30 2021-04-21 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
WO2019012095A1 (en) * 2017-07-14 2019-01-17 C-Lecta Gmbh TCO-reductase
CN107832577B (zh) * 2017-10-30 2021-07-13 中国农业大学 一种筛选几丁质酶OfChtⅠ抑制剂的方法
CN107974484A (zh) * 2017-11-10 2018-05-01 嘉兴欣贝莱生物科技有限公司 根皮素生物合成过程中查尔酮合成酶建模方法
KR102604438B1 (ko) * 2018-03-05 2023-11-21 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 머신 러닝 및 분자 시뮬레이션에 기반한 결합 및 활성 예측 향상 방법
EP3613855A1 (en) * 2018-08-23 2020-02-26 Clariant Produkte (Deutschland) GmbH Method for the production of a nucleic acid library
WO2020055246A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
EP3640864A1 (en) * 2018-10-18 2020-04-22 Fujitsu Limited A computer-implemented method and apparatus for inferring a property of a biomedical entity
MX2021004925A (es) 2018-10-29 2021-06-15 Codexis Inc Variantes de adn-polimerasa modificadas.
SG11202105668PA (en) 2018-12-14 2021-06-29 Codexis Inc Engineered tyrosine ammonia lyase
JP2022515742A (ja) 2018-12-20 2022-02-22 コデクシス， インコーポレイテッド α－ガラクトシダーゼバリアント
CN109841263B (zh) * 2019-02-22 2023-08-15 成都分迪科技有限公司 蛋白降解药物分子库及其构建方法
JP7344509B2 (ja) * 2019-09-20 2023-09-14 公立大学法人 富山県立大学 光学活性フルオロアルコールおよび光学活性クロロフルオロアルコールの製造方法
US20230227797A1 (en) * 2019-10-28 2023-07-20 Asymchem Laboratories (Tianjin) Co., Ltd. Transaminase mutant and use thereof
JP2023507575A (ja) 2019-12-20 2023-02-24 コデクシス， インコーポレイテッド 操作された酸性アルファ－グルコシダーゼバリアント
CN111681703A (zh) * 2020-05-09 2020-09-18 北京纽伦智能科技有限公司 一种蛋白结构的对接方法及分布式蛋白结构对接系统
CN114822717A (zh) * 2021-01-28 2022-07-29 腾讯科技（深圳）有限公司 基于人工智能的药物分子处理方法、装置、设备及存储介质
WO2023022783A1 (en) * 2021-08-17 2023-02-23 University Of Southern California System and method for computational enzyme design based on maximum entropy
CN113921082B (zh) * 2021-10-27 2023-04-07 云舟生物科技(广州)股份有限公司 基因搜索权重调整方法、计算机存储介质及电子设备
CN117511725B (zh) * 2023-12-21 2024-09-20 北京康润诚业生物科技有限公司 一种酶分子定向进化生物信息分析装置及方法

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPH04179495A (ja) 1990-11-14 1992-06-26 M D Res Kk 融合蛋白質、およびペプチド又は蛋白質の製造方法
US5426039A (en) 1993-09-08 1995-06-20 Bio-Rad Laboratories, Inc. Direct molecular cloning of primer extended DNA containing an alkane diol
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
DK1717322T3 (da) 1997-01-17 2012-10-22 Codexis Mayflower Holdings Llc Udvikling af hele celler og organismer ved rekursiv sekvensrekombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1151409A1 (en) 1999-01-18 2001-11-07 Maxygen, Inc. Methods of populating data stuctures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US20070065838A1 (en) 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
AU3210100A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
CA2364997A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Encryption of traits using split gene sequences
US6969763B1 (en) * 1999-05-12 2005-11-29 Isis Pharmaceuticals, Inc. Molecular interaction sites of interleukin-2 RNA and methods of modulating the same
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
WO2001071347A1 (en) * 2000-03-23 2001-09-27 California Institute Of Technology Method and apparatus for predicting ligand binding interactions
WO2001075767A2 (en) 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
US20020133297A1 (en) * 2001-01-17 2002-09-19 Jinn-Moon Yang Ligand docking method using evolutionary algorithm
WO2002083868A2 (en) 2001-04-16 2002-10-24 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
DK2278509T3 (en) 2002-03-01 2014-12-15 Codexis Mayflower Holdings Llc Methods, systems and software for identification of functional biomolecules
US7620500B2 (en) 2002-03-09 2009-11-17 Maxygen, Inc. Optimization of crossover points for directed evolution
AU2003276548A1 (en) * 2002-10-28 2004-05-13 Centre National De La Recherche Scientifique (C.N.R.S.) A method for performing restrained dynamics docking of one or multiple substrates on multi-specific enzymes
US20060121455A1 (en) * 2003-04-14 2006-06-08 California Institute Of Technology COP protein design tool
CN1468959A (zh) 2003-06-02 2004-01-21 复旦大学 非典型性肺炎冠状病毒蛋白质空间构象模型及其应用
US7524664B2 (en) 2003-06-17 2009-04-28 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8005620B2 (en) 2003-08-01 2011-08-23 Dna Twopointo Inc. Systems and methods for biopolymer engineering
EP1660646B1 (en) 2003-08-11 2014-12-31 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
JP2005309877A (ja) 2004-04-22 2005-11-04 National Institute Of Advanced Industrial & Technology 機能性生体分子の配列解析方法
WO2006044378A2 (en) 2004-10-12 2006-04-27 University Of Iowa Research Foundation Rapid computational identification of targets
WO2006121455A1 (en) 2005-05-10 2006-11-16 The Salk Institute For Biological Studies Dynamic signal processing
CA2638905A1 (en) * 2006-01-23 2007-08-02 Joseph P. Errico Methods and compositions of targeted drug development
RU2008140858A (ru) 2006-03-15 2010-04-20 Ксир (Za) Способ скрининга соединений, обладающих активностью ингибитора глутамин синтетазы
CA2667974A1 (en) 2006-07-05 2008-01-10 The Scripps Research Institute Chimeric zinc finger recombinases optimized for catalysis by directed evolution
US7814234B2 (en) 2006-10-30 2010-10-12 Microsoft Corporation Offline execution of web based applications
US7820421B2 (en) 2007-02-08 2010-10-26 Codexis, Inc. Ketoreductases and uses thereof
SG178753A1 (en) 2007-02-12 2012-03-29 Codexis Inc Structure-activity relationships
CN101784669B (zh) 2007-08-24 2015-02-18 科德克希思公司 用于(r)-3-羟基四氢噻吩的立体选择性制备的改善的酮还原酶多肽
JP5973131B2 (ja) 2007-09-13 2016-08-23 コデクシス， インコーポレイテッド アセトフェノンの還元のためのケトレダクターゼポリペプチド
CN101889081B (zh) 2007-09-28 2014-06-18 科德克希思公司 酮还原酶多肽及其用途
JP5646328B2 (ja) 2007-10-01 2014-12-24 コデクシス， インコーポレイテッド アゼチジノンの生産のためのケトレダクターゼポリペプチド
CN101889074A (zh) 2007-10-04 2010-11-17 哈尔西恩莫尔丘勒公司 采用电子显微镜对核酸聚合物测序
EP2216429A4 (en) 2007-11-12 2011-06-15 In Silico Sciences Inc SILICO SCREENING SYSTEM AND SILICO SCREENING METHOD
WO2009152336A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
CN102131813B (zh) 2008-06-24 2014-07-30 科德克希思公司 用于制备基本上立体异构纯的稠合二环脯氨酸化合物的生物催化方法
PT2315773T (pt) 2008-07-25 2016-11-23 Glaxosmithkline Biologicals Sa Polipéptidos, polinucleótidos e composições para utilização no tratamento de tuberculose latente
EP2329013B1 (en) 2008-08-27 2015-10-28 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8247192B2 (en) 2008-11-10 2012-08-21 Codexis, Inc. Penicillin-G acylases
WO2010077470A2 (en) * 2008-11-19 2010-07-08 University Of Washington Enzyme catalysts for diels-alder reactions
WO2010080635A2 (en) 2008-12-18 2010-07-15 Codexis, Inc. Recombinant halohydrin dehalogenase polypeptides
WO2010075574A2 (en) 2008-12-25 2010-07-01 Codexis, Inc. Enone reductases
DK3354727T3 (da) 2009-01-08 2020-11-16 Codexis Inc Transaminasepolypeptider
EP2401366B1 (en) 2009-02-26 2013-12-18 Codexis, Inc. Transaminase biocatalysts
WO2011011630A2 (en) 2009-07-23 2011-01-27 Codexis, Inc. Nitrilase biocatalysts
SI2510089T1 (sl) 2009-12-08 2015-12-31 Codexis, Inc. Sinteza prazolnih spojin
WO2011082310A2 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
HUE037616T2 (hu) 2010-12-08 2018-09-28 Codexis Inc Biokatalizátorok és eljárások armodafinil szintéziséhez
CN102156823B (zh) 2011-02-18 2015-04-22 复旦大学 一种靶向作用于蛋白激酶非活性构象的化合物筛选方法
EP2866811A1 (en) 2012-06-29 2015-05-06 Wisconsin Alumni Research Foundation USE OF 2-METHYLENE-19-NOR-(20S)-1 alpha,25-DIHYDROXYVITAMIN D³ TO TREAT SECONDARY HYPERPARATHYROIDISM
US20140303952A1 (en) 2013-04-08 2014-10-09 City University Of Hong Kong Protein-ligand docking
CN103265635A (zh) 2013-04-28 2013-08-28 中山大学附属第一医院 一种通用的靶向蛋白嵌合型分子化合物的构建方法
CN103324861B (zh) 2013-07-10 2016-07-20 南京大学 基于分子动力学模拟的核受体介导内分泌干扰物质的虚拟筛选方法
KR102341026B1 (ko) 2013-09-27 2021-12-21 코덱시스, 인코포레이티드 구조에 기반한 예측 모델링
KR102342205B1 (ko) 2013-09-27 2021-12-21 코덱시스, 인코포레이티드 효소 변이체의 자동화 스크리닝

Also Published As

Publication number Publication date
IL244457B (en) 2021-05-31
ES2693150T3 (es) 2018-12-07
US11535845B2 (en) 2022-12-27
JP2016537700A (ja) 2016-12-01
DK3418929T3 (da) 2021-02-01
BR112016006285B1 (pt) 2022-09-06
EP3418929A1 (en) 2018-12-26
DK3049973T3 (en) 2018-10-22
JP6857029B2 (ja) 2021-04-14
CN105765592B (zh) 2019-12-17
RU2019140645A (ru) 2020-01-27
US20230048421A1 (en) 2023-02-16
EP3418929B1 (en) 2020-12-09
KR102342205B1 (ko) 2021-12-21
KR20160057482A (ko) 2016-05-23
RU2016116253A (ru) 2017-11-01
US20150133307A1 (en) 2015-05-14
JP2021131901A (ja) 2021-09-09
US20200277597A1 (en) 2020-09-03
CN105765592A (zh) 2016-07-13
AU2014324669A1 (en) 2016-03-24
NZ717658A (en) 2020-11-27
AU2014324669B2 (en) 2020-06-04
ES2857711T3 (es) 2021-09-29
SG11201601695WA (en) 2016-04-28
US10696964B2 (en) 2020-06-30
EP3049973A1 (en) 2016-08-03
EP3049973B1 (en) 2018-08-08
CA2923755A1 (en) 2015-04-02
JP2019083025A (ja) 2019-05-30
HUE053049T2 (hu) 2021-06-28
IL244457A0 (en) 2016-04-21
WO2015048572A1 (en) 2015-04-02
HUE039618T2 (hu) 2019-01-28
CA2923755C (en) 2023-03-14

Similar Documents

Publication Publication Date Title
BR112016006285A2 (pt) método implementado usando um sistema de computador, produto de programa de computador, e, sistema
BR112015018449A2 (pt) método para identificar moléculas biológicas para afetar uma atividade desejada e sistema de computação
GB2569252A (en) Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
MX2016016713A (es) Procesos y sistemas para el montaje de secuencias de acido nucleico.
EP4219745A3 (en) Spatially encoded biological assays using a microfluidic device
BR112018011567A2 (pt) método de purificação de uma proteína de interesse, e, uso de um método.
BR112016006284A2 (pt) método para conduzir evolução direcionada, produto de programa de computador, e, sistema de computador
MX2021003772A (es) Modulacion de globulos de polimero para procesamiento de adn.
MX2016007541A (es) Moléculas modificadas de ácido nucleico y usos de las mismas.
BR122022007092B8 (pt) Método para construir um perfil de dna, método para construir uma biblioteca de ácido nucléico, biblioteca de ácido nucléico, pluralidade de iniciadores e kit
BR112019001194A2 (pt) sistemas e métodos para preparar e coprocessar óleo biocru
BR112015006255A2 (pt) Métodos para a produção de produtos químicos e derivados dos mesmos
BR112013001421A2 (pt) método para a iluminação controlada de uma área com um sistema de iluminação, produto de programa de computador, sistema de iluminação e sistema de iluminação
MX2015001939A (es) Composiciones y metodos para microcapsulas.
EP3721377A4 (en) SYSTEMS AND PROCEDURES FOR EFFICIENT PERFORMANCE OF BIOLOGICAL TESTS
BR112017004029A2 (pt) produção de poliolefinas com catalisadores à base de cromo
EA201790504A1 (ru) Монослой мононуклеарных клеток периферической крови или клеток костного мозга и его применение
EP4220399A3 (en) Dynamic reconfiguration of applications on a multi-processor embedded system
BR112014018761A8 (pt) Método para reduzir tempos de inicialização de plataforma por proporcionar abstrações lentas de entrada/saída
NZ734644A (en) Chemically encoded spatially addressed library screening platforms
BR112012000273A2 (pt) método de processamento de sinais respiratórios, sistema de processamento de sinais respiratórios, programa de computador ou produto de programa de computador para realizar o método e transportador
BR112013017363A2 (pt) biblioteca de alphabody de cadeia única, mistura de bibliotecas de alphabody, ácido nucleico ou biblioteca de vetor, biblioteca de células hospedeiras, uso de uma biblioteca de alphabody de cadeia única, método para a produção de uma biblioteca de alphabody de cadeia única, método para a produção de um ou mais polipeptídeos de alphabody de cadeia única, polipeptíceo de alphabody de cadeia única, ácido nucleico, vetor e célula hospedeira
MX2015012319A (es) Sistemas de instrumento analitico.
EP3607575A4 (en) SYSTEMS AND METHODS FOR CONDUCTING REACTIONS AND SCREENING REACTION PRODUCTS
BR112015024134A2 (pt) método para classificar a amostra biológica, sistema para classificar a amostra biológica e produto de programa de computador

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: G06F 19/00 , G06F 19/16

Ipc: C12N 15/10 (2006.01), G16B 35/00 (2006.01), G16B 3

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 26/09/2014, OBSERVADAS AS CONDICOES LEGAIS