BR112015025489A2 - fosfocetolases para produção melhorada de metabolitos derivados de acetil coenzima a, isopreno, precursores de isoprenoides, e isoprenoides - Google Patents

fosfocetolases para produção melhorada de metabolitos derivados de acetil coenzima a, isopreno, precursores de isoprenoides, e isoprenoides

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Publication number
BR112015025489A2
BR112015025489A2 BR112015025489A BR112015025489A BR112015025489A2 BR 112015025489 A2 BR112015025489 A2 BR 112015025489A2 BR 112015025489 A BR112015025489 A BR 112015025489A BR 112015025489 A BR112015025489 A BR 112015025489A BR 112015025489 A2 BR112015025489 A2 BR 112015025489A2
Authority
BR
Brazil
Prior art keywords
isoprene
acetyl coenzyme
precursors
production
phosphocetolases
Prior art date
Application number
BR112015025489A
Other languages
English (en)
Inventor
H Wells Derek
W Munos Jeffrey
Yao Jian
Q Beck Zachary
Original Assignee
Danisco Us Inc
Goodyear Tire & Rubber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
Publication of BR112015025489A2 publication Critical patent/BR112015025489A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/02009—Phosphoketolase (4.1.2.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

abstract this present invention relates to cultured recombinant cells comprising a heterologous phosphoketolase (pkl) polypeptide that are capable of increased production of acetyl coenzyme a-derived metabolites, as well as methods for producing and using the same. in some embodiments, the recombinant cells further comprise one or more mevalonate (mva) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids. tradução do resumo resumo patente de invenção para "fosfocetolases para produção melhorada de metabolitos derivados de acetil coenzima a, isopreno, precursores de isoprenoides, e isoprenoides" esta presente invenção se relaciona com células recombinantes cultivadas compreendendo um polipeptídeo de fosfocetolase (pkl) heterólogo que é capaz de produção aumentada de metabolitos derivados de acetil coenzima a, bem como métodos para produção e uso do mesmo. em algumas formas de realização, as células recombinantes compreendem adicionalmente um ou mais polipeptídeos da via do mevalonato (mva) para a produção de precursores de isoprenoides, isopreno e isoprenoides.
BR112015025489A 2013-04-10 2014-04-10 fosfocetolases para produção melhorada de metabolitos derivados de acetil coenzima a, isopreno, precursores de isoprenoides, e isoprenoides BR112015025489A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361810696P 2013-04-10 2013-04-10
US201361834359P 2013-06-12 2013-06-12
PCT/US2014/033688 WO2014169144A2 (en) 2013-04-10 2014-04-10 Phosphoketolases foe improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precurosors, and isoprenoids

Publications (1)

Publication Number Publication Date
BR112015025489A2 true BR112015025489A2 (pt) 2017-10-10

Family

ID=50877659

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015025489A BR112015025489A2 (pt) 2013-04-10 2014-04-10 fosfocetolases para produção melhorada de metabolitos derivados de acetil coenzima a, isopreno, precursores de isoprenoides, e isoprenoides

Country Status (8)

Country Link
US (3) US10246694B2 (pt)
EP (2) EP3425049A1 (pt)
JP (2) JP6530375B2 (pt)
CN (1) CN105555952A (pt)
BR (1) BR112015025489A2 (pt)
ES (1) ES2735331T3 (pt)
SG (1) SG11201508186XA (pt)
WO (1) WO2014169144A2 (pt)

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US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
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EP3720944A1 (en) 2017-12-07 2020-10-14 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation
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WO2021063958A1 (en) * 2019-09-30 2021-04-08 Global Bioenergies Improved means and methods for increasing the yield of acetyl-coa from glucose

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Also Published As

Publication number Publication date
WO2014169144A3 (en) 2014-12-04
SG11201508186XA (en) 2015-11-27
US20190367896A1 (en) 2019-12-05
JP6530375B2 (ja) 2019-06-12
ES2735331T3 (es) 2019-12-18
US10246694B2 (en) 2019-04-02
US20160068831A1 (en) 2016-03-10
WO2014169144A8 (en) 2015-10-15
EP2984105B1 (en) 2019-05-22
EP3425049A1 (en) 2019-01-09
US11371035B2 (en) 2022-06-28
EP2984105A2 (en) 2016-02-17
CN105555952A (zh) 2016-05-04
US20210139878A1 (en) 2021-05-13
JP2016519577A (ja) 2016-07-07
WO2014169144A2 (en) 2014-10-16
JP2019162130A (ja) 2019-09-26
US10988750B2 (en) 2021-04-27

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B154 Notification of filing of divisional application [chapter 15.50 patent gazette]

Free format text: O PEDIDO FOI DIVIDIDO NO BR122023000309-8 PROTOCOLO 870230001753 EM 06/01/2023 15:34.

B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]