AR093289A1 - Polinucleotidos, polipeptidos mejorados de aciltransferasa y sus metodos de uso - Google Patents

Polinucleotidos, polipeptidos mejorados de aciltransferasa y sus metodos de uso

Info

Publication number
AR093289A1
AR093289A1 ARP130103955A ARP130103955A AR093289A1 AR 093289 A1 AR093289 A1 AR 093289A1 AR P130103955 A ARP130103955 A AR P130103955A AR P130103955 A ARP130103955 A AR P130103955A AR 093289 A1 AR093289 A1 AR 093289A1
Authority
AR
Argentina
Prior art keywords
modified
proteins
cells
methods
dgat1
Prior art date
Application number
ARP130103955A
Other languages
English (en)
Original Assignee
Agresearch Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agresearch Ltd filed Critical Agresearch Ltd
Publication of AR093289A1 publication Critical patent/AR093289A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats

Abstract

La solicitud proporciona proteínas DGAT1 modificadas que están modificadas en la región N-terminal corriente arriba del sitio de unión de acil-CoA. Las proteínas DGAT modificadas muestran actividad diferente, sin reducción de la acumulación de proteína cuando se expresan en las células. Las proteínas DGAT1 modificadas de la solicitud se pueden expresar en las células para aumentar la acumulación de lípidos celulares y/o modificar el perfil de los lípidos celulares. También proporciona polinucleótidos que codifican las proteínas DGAT1 modificadas, células y composiciones que comprenden los polinucleótidos o proteínas DGAT1 modificadas, y métodos de usar las proteínas DGAT1 modificadas para producir aceite.
ARP130103955A 2012-10-30 2013-10-30 Polinucleotidos, polipeptidos mejorados de aciltransferasa y sus metodos de uso AR093289A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261720069P 2012-10-30 2012-10-30

Publications (1)

Publication Number Publication Date
AR093289A1 true AR093289A1 (es) 2015-05-27

Family

ID=50628188

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130103955A AR093289A1 (es) 2012-10-30 2013-10-30 Polinucleotidos, polipeptidos mejorados de aciltransferasa y sus metodos de uso

Country Status (11)

Country Link
US (1) US11312971B2 (es)
EP (1) EP2914727A4 (es)
CN (1) CN105473718A (es)
AR (1) AR093289A1 (es)
AU (1) AU2013340443B2 (es)
BR (1) BR112015009455A2 (es)
CA (1) CA2889800A1 (es)
IN (1) IN2015DN04034A (es)
MX (1) MX363271B (es)
NZ (1) NZ706832A (es)
WO (1) WO2014068437A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX354445B (es) 2012-10-30 2018-03-06 Agresearch Ltd Polinucleotidos, polipeptidos y metodos de uso novedoso de aciltransferasa.
WO2014068438A2 (en) 2012-10-30 2014-05-08 Agresearch Limited Novel acyltransferase polynucleotides, polypeptides, and methods of use
CN104884622B (zh) 2012-12-21 2018-11-13 新西兰植物和食品研究院有限公司 基因表达的调控
US10472587B2 (en) 2014-07-07 2019-11-12 Commonwealth Scientific And Industrial Research Organisation Processes for producing industrial products from plant lipids
MX2019002483A (es) 2016-09-02 2019-09-09 Commw Scient Ind Res Org Plantas con rasgos modificados.
US11479759B2 (en) 2018-06-01 2022-10-25 Pioneer Hi-Bred International, Inc. Modified seed oil content by gene editing
WO2023111871A1 (en) * 2021-12-16 2023-06-22 Agresearch Limited Modified acyltransferase polynucleotides, polypeptides, and methods of use

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US4795855A (en) 1985-11-14 1989-01-03 Joanne Fillatti Transformation and foreign gene expression with woody species
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5750871A (en) 1986-05-29 1998-05-12 Calgene, Inc. Transformation and foreign gene expression in Brassica species
US5187073A (en) 1986-06-30 1993-02-16 The University Of Toledo Process for transforming gramineae and the products thereof
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5639952A (en) 1989-01-05 1997-06-17 Mycogen Plant Science, Inc. Dark and light regulated chlorophyll A/B binding protein promoter-regulatory system
US5086169A (en) 1989-04-20 1992-02-04 The Research Foundation Of State University Of New York Isolated pollen-specific promoter of corn
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
DE69132124T2 (de) 1990-11-23 2000-11-23 Aventis Cropscience Nv Verfahren zur transformation monokotyler pflanzen
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
EP0651814B1 (en) 1992-07-09 1997-01-08 Pioneer Hi-Bred International, Inc. Maize pollen-specific polygalacturonase gene
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5545169A (en) 1993-04-01 1996-08-13 Yarger; Richard J. Laparoscopic delivery device
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
US5792935A (en) 1993-12-09 1998-08-11 Texas A&M University Agrobacterium tumefaciens transformation of Musa species
GB9421286D0 (en) 1994-10-21 1994-12-07 Danisco Promoter
US5536653A (en) 1994-11-04 1996-07-16 Monsanto Company Tomato fruit promoters
US5846797A (en) 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
US6127179A (en) 1996-04-17 2000-10-03 Dellapenna; Dean Gene promoter for tomato fruit
DE19644478A1 (de) 1996-10-25 1998-04-30 Basf Ag Blattspezifische Expression von Genen in transgenen Pflanzen
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5952543A (en) 1997-02-25 1999-09-14 Dna Plant Technology Corporation Genetically transformed pineapple plants and methods for their production
US5968830A (en) 1997-03-28 1999-10-19 Mississippi State University Soybean transformation and regeneration methods
WO1998055631A1 (en) 1997-06-05 1998-12-10 Calgene Llc Diacylglycerol acyl transferase proteins
US6037522A (en) 1998-06-23 2000-03-14 Rhone-Poulenc Agro Agrobacterium-mediated transformation of monocots
US6344548B1 (en) 1998-06-24 2002-02-05 The Regents Of The University Of California Diacylglycerol o-acyltransferase
US7135617B2 (en) 1998-07-02 2006-11-14 Calgene Llc Diacylglycerol acyl transferase proteins
JP4514952B2 (ja) 1998-07-02 2010-07-28 カルジーン エルエルシー ジアシルグリセロールアシルトランスフェラーゼタンパク質
US6100077A (en) 1998-10-01 2000-08-08 The Trustees Of Columbia University In The City Of New York Isolation of a gene encoding diacylglycerol acyltransferase
EP1135473A2 (en) * 1998-12-02 2001-09-26 E.I. Dupont De Nemours And Company Sequenzes of a putative plant diacylglycerol acyltransferases
US6342657B1 (en) 1999-05-06 2002-01-29 Rhone-Poulenc Agro Seed specific promoters
US7560542B2 (en) 1999-05-07 2009-07-14 Monsanto Technology Llc Nucleic acid molecule SEQ ID NO. 68811 and other molecules associated with plants
US7642346B2 (en) 1999-08-27 2010-01-05 Sembiosys Genetics Inc. Flax seed specific promoters
MXPA02007130A (es) 2000-01-21 2002-12-13 Pioneer Hi Bred Int Elementos promotores novedosos preferidos de raiz y metodos de uso..
AU2001291656A1 (en) 2000-06-30 2002-01-08 Willem Broekaert Gene silencing vector
CA2430642A1 (en) 2000-12-01 2003-02-20 John B. Ohlrogge Plant seed specific promoters
US20040067506A1 (en) 2000-12-04 2004-04-08 Ben Scheres Novel root specific promoter driving the expression of a novel lrr receptor-like kinase
EP1256629A1 (en) 2001-05-11 2002-11-13 Société des Produits Nestlé S.A. Leaf specifc gene promoter of coffee
WO2004011671A2 (en) 2002-07-31 2004-02-05 Monsanto Technology, Llc Diacylglycerol acyltransferase nucleic acid sequences and associated products
WO2004044205A1 (en) 2002-11-11 2004-05-27 Korea Chungang Educational Foundation Plant seed-specific expression promoter derived from sesame and seed-specific expression vector comprising the promoter
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
EP1528104A1 (en) 2003-11-03 2005-05-04 Biogemma MEG1 endosperm-specific promoters and genes
WO2005100575A2 (en) 2004-04-14 2005-10-27 Bayer Bioscience N.V. Rice pollen-specific promoters and uses thereof
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
MY187548A (en) 2005-06-13 2021-09-29 Government Of Malaysia As Represented By The Mini Of Science Tehnology And Innovation Malaysia Leaf-specific chlorophyll a/b binding protein promoter
US8847010B2 (en) * 2007-06-15 2014-09-30 Biotechnology Foundation, Inc. Engineered tobacco biomass with increased oil production
US8299318B2 (en) 2007-07-05 2012-10-30 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
US8362325B2 (en) 2007-10-03 2013-01-29 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
US20110190165A1 (en) * 2008-05-16 2011-08-04 Randall Weselake High throughput methods of identifying neutral lipid synthases
EP2297310A2 (en) 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
BR112012011464A2 (pt) 2009-10-30 2015-09-15 Agres Ltd proteínas encapsuladas de óleo modificadas e seu uso
CN101824416B (zh) * 2010-04-30 2011-05-11 湖南农业大学 猪肉质性状相关基因dgat1及其在猪标记辅助选择中的应用
EP2823042A1 (en) * 2012-03-09 2015-01-14 Matrix Genetics, LLC Modified diacylglycerol acyltransferase proteins and methods of use thereof
WO2014068438A2 (en) * 2012-10-30 2014-05-08 Agresearch Limited Novel acyltransferase polynucleotides, polypeptides, and methods of use
MX354445B (es) 2012-10-30 2018-03-06 Agresearch Ltd Polinucleotidos, polipeptidos y metodos de uso novedoso de aciltransferasa.

Also Published As

Publication number Publication date
WO2014068437A3 (en) 2014-06-26
US11312971B2 (en) 2022-04-26
US20150275223A1 (en) 2015-10-01
IN2015DN04034A (es) 2015-10-02
BR112015009455A2 (pt) 2017-11-14
CA2889800A1 (en) 2014-05-08
AU2013340443B2 (en) 2019-06-06
AU2013340443A1 (en) 2015-05-07
NZ706832A (en) 2019-04-26
EP2914727A4 (en) 2016-04-20
EP2914727A2 (en) 2015-09-09
MX363271B (es) 2019-03-19
WO2014068437A2 (en) 2014-05-08
CN105473718A (zh) 2016-04-06
MX2015005260A (es) 2015-07-17

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