BR112015000836B1 - Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras - Google Patents

Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras Download PDF

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BR112015000836B1
BR112015000836B1 BR112015000836-4A BR112015000836A BR112015000836B1 BR 112015000836 B1 BR112015000836 B1 BR 112015000836B1 BR 112015000836 A BR112015000836 A BR 112015000836A BR 112015000836 B1 BR112015000836 B1 BR 112015000836B1
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cells
binding
sequence
antibody
expression
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BR112015000836-4A
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BR112015000836A2 (pt
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Ulf Grawunder
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Nbe-Therapeutics Llc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112015000836-4A 2012-07-18 2013-07-18 Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras BR112015000836B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261673144P 2012-07-18 2012-07-18
US61/673,144 2012-07-18
EP12178529-9 2012-07-30
EP12178529.9A EP2692865B1 (en) 2012-07-30 2012-07-30 Transposition-mediated identification of specific binding or functional proteins
PCT/EP2013/065214 WO2014013026A1 (en) 2012-07-18 2013-07-18 Transposition-mediated identification of specific binding or functional proteins

Publications (2)

Publication Number Publication Date
BR112015000836A2 BR112015000836A2 (pt) 2017-12-12
BR112015000836B1 true BR112015000836B1 (pt) 2021-12-21

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BR112015000836-4A BR112015000836B1 (pt) 2012-07-18 2013-07-18 Método para identificar um polipeptídeo tendo uma funcionalidade ou especificidade de ligação desejadas, biblioteca de moléculas de polinucleotídeo, método para gerar uma biblioteca de polinucleotídeos e método para gerar uma população de células hospedeiras

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Country Link
US (2) US10174309B2 (https=)
EP (2) EP2692865B1 (https=)
JP (1) JP6043431B2 (https=)
CN (1) CN104640985B (https=)
AU (1) AU2013291971B2 (https=)
BR (1) BR112015000836B1 (https=)
CA (1) CA2878982C (https=)
DK (1) DK2692865T3 (https=)
ES (1) ES2528892T3 (https=)
IL (1) IL236771A (https=)
IN (1) IN2015DN01301A (https=)
MX (1) MX338911B (https=)
NZ (1) NZ705074A (https=)
PT (1) PT2692865E (https=)
SG (1) SG11201500280XA (https=)
SI (1) SI2692865T1 (https=)
WO (1) WO2014013026A1 (https=)
ZA (1) ZA201500203B (https=)

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Also Published As

Publication number Publication date
EP2692865B1 (en) 2014-12-10
JP2015522286A (ja) 2015-08-06
HK1209779A1 (en) 2016-04-08
US20190153433A1 (en) 2019-05-23
IN2015DN01301A (https=) 2015-07-03
ZA201500203B (en) 2015-12-23
AU2013291971A1 (en) 2015-02-05
CN104640985B (zh) 2021-05-11
MX2015000885A (es) 2015-07-14
IL236771A (en) 2016-09-29
BR112015000836A2 (pt) 2017-12-12
NZ705074A (en) 2017-04-28
SG11201500280XA (en) 2015-02-27
EP2692865A1 (en) 2014-02-05
EP2687599A1 (en) 2014-01-22
US20150152406A1 (en) 2015-06-04
ES2528892T3 (es) 2015-02-13
WO2014013026A1 (en) 2014-01-23
CN104640985A (zh) 2015-05-20
SI2692865T1 (sl) 2015-03-31
MX338911B (es) 2016-05-05
CA2878982C (en) 2017-01-03
JP6043431B2 (ja) 2016-12-14
US10174309B2 (en) 2019-01-08
PT2692865E (pt) 2015-02-06
CA2878982A1 (en) 2014-01-23
WO2014013026A8 (en) 2014-12-04
DK2692865T3 (en) 2015-02-16
AU2013291971B2 (en) 2016-02-04

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